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zero123


Registered: 01/03/12
Posts: 22
Last seen: 7 years, 1 month
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Can someone please tell me things are going well?
#23781509 - 10/28/16 10:49 PM (7 years, 3 months ago) |
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Let's get right down to it. Here's the FC: There's a total of 5 jars, but here's 4 of them looked ok:  Although 'ok' is the word used, there are these spots of discoloration that are upsetting. notice the weird color: 

Here's the jar that's been isolated. Look at the grayish parts. But also look how much faster it's colonizing. Maybe it's just mycelium pushed up against the glass?


Must admit that worry is creeping in. Maybe the isolated jar is okay but the ones that have the strange tint on the uncolonized parts are bad? They're all gonna be left to continue until overwhelming evidence of contam is showing
Opinions and comments are greatly appreciated!
Edited by zero123 (10/28/16 10:55 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781533 - 10/28/16 11:08 PM (7 years, 3 months ago) |
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all the green ones need to be dumped in the trash.
the third one in the first picture the one on the right in the 2nd the one on the right in the 3rd the one in the front of the 4th and everything at least in the angle it's at in the 5th picture looks ok
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zero123


Registered: 01/03/12
Posts: 22
Last seen: 7 years, 1 month
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781552 - 10/28/16 11:23 PM (7 years, 3 months ago) |
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DAMN IT. so heartbreaking. are you sure the mycelium won't fight back? the jars that have the nastyness in them have really healthy growth everywhere else. maybe it will eventually overpower the contam in a few jars? to only have 2 cakes fruiting in this chamber that fits 6 comfortably... shameful.
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freespeech
disciple



Registered: 12/12/08
Posts: 1,745
Loc: PNW
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781560 - 10/28/16 11:28 PM (7 years, 3 months ago) |
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Quote:
zero123 said: DAMN IT. so heartbreaking. are you sure the mycelium won't fight back? the jars that have the nastyness in them have really healthy growth everywhere else. maybe it will eventually overpower the contam in a few jars? to only have 2 cakes fruiting in this chamber that fits 6 comfortably... shameful.
Haha, no dude. Those green jars are not "ok" in any sense of the word - they're done. "Fighting back" is what you hope for when your jars are ill with a touch of bacteria; your jars are straight up dead. Don't even think about trying to fruit any of them. Go bury them in your garden if you want a shot at something happening from them, but don't keep that mold in your house for any longer than you need to.
Figure out where your technique went wrong and correct it for next time. In your case it's either improper sterilization of the substrate, or a dirty syringe. Most likely the former. How did you sterilize your cakes?
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zero123 said: They're all gonna be left to continue until overwhelming evidence of contam is showing
It's staring you right in the face and couldn't be more obvious. Sorry!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can someone please tell me things are going well? [Re: freespeech]
#23781584 - 10/28/16 11:45 PM (7 years, 3 months ago) |
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https://www.shroomery.org/forums/showflat.php/Number/23781472#23781472 https://www.shroomery.org/forums/showflat.php/Number/23776463#23776463
molds die well before boiling temperatures are reached, so unless you completely skipped sterilization it was your sterile technique during inoculation or it was the syringe.
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zero123


Registered: 01/03/12
Posts: 22
Last seen: 7 years, 1 month
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Re: Can someone please tell me things are going well? [Re: freespeech]
#23781596 - 10/28/16 11:54 PM (7 years, 3 months ago) |
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you're right, i'm just...ugh! hopefully the other 2 jars are ok after leaving them with the bad ones for sooo long. they seem fine but the one has the tiniest amount of green tinge in one area. FUCK. gonna separate the remaining 2 just in case one of them is compromised.
took the other 3 contam jars and double wrapped them in garbage bags. never thought about burying them in the yard, that would be so cool if something came from them haha.
there's a few times the process may have been fucked up: - 2 of the jars were slightly crooked in the pot while boiling, due to trying to fit more than i should have in the pot, which may have led to moisture getting into the dry verm layer - did not use a glove box for inoculation. - yellow kitchen gloves were used instead of regular latex (does it matter?) - did not wear a face mask - used a cig lighter for sterilizing needle red hot, but maybe not enough of the needle was made red hot? maybe the cig lighter is a no-no? - did not wipe the needle on alcohol-soaked paper towel between jars, even though i had the damn paper towel sitting right nearby... - had the jars lightly covered with a paper towel to maybe add an extra layer of protection, but then just threw it away when i read that open air and ambient light is good for the mycelium. - should i have left them in a dark place and never touch or see them at all for about 2 or 3 weeks, then check them? i guess its possible that just sitting out in the room exposed to all the dust getting kicked up as i walk around each day is not good? -
wish me luck for next time guys...*sigh* suppose it's still possible to get something assuming these 2 babies don't die on me
Edited by zero123 (10/29/16 12:04 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781601 - 10/28/16 11:58 PM (7 years, 3 months ago) |
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sounds like the syringe was bad or the dry verm barrier wasn't done right
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freespeech
disciple



Registered: 12/12/08
Posts: 1,745
Loc: PNW
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781619 - 10/29/16 12:11 AM (7 years, 3 months ago) |
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Quote:
zero123 said: - 2 of the jars were slightly crooked in the pot while boiling, due to trying to fit more than i should have in the pot, which may have led to moisture getting into the dry verm layer
Maybe. The dry verm layer is there to protect you from the sloppy technique that accompanies new growers who are trying out the BRF tek. Respect the dry verm layer.
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zero123 said: - did not use a glove box for inoculation.
With the dry verm layer, this is not that big of a deal, IF (big if) you are smart about your technique. If you did this in a breezy, carpeted room, with a dog sniffing around at your heels during the procedure, then you fucked up. That said, use a still air box next time if you are able. You don't need anything fancy; the most basic plastic tote-turned-over will do.
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zero123 said: - yellow kitchen gloves were used instead of regular latex (does it matter?)
Probably fine. Lather up with rubbing alcohol frequently during inoculation. I put 70% iso in a spray bottle and liberally and repeatedly spray my hands and forearms whenever I'm doing something that requires sterility.
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zero123 said: - did not wear a face mask
If you are smart about it, this may not matter that much. As a new grower, I don't count on you to be smart about it. Buy some masks from the drug store if you want to do it right. In general, you're going to see bacteria problems as a result of breathing on your jar, rather than the mold that you have.
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zero123 said: - used a cig lighter for sterilizing needle red hot, but maybe not enough of the needle was made red hot? maybe the cig lighter is a no-no?
Heat source doesn't matter as long as you do what it takes to kill bad stuff that's in the needle. A gas torch works much better, though.
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zero123 said: - had the jars lightly covered with a paper towel to maybe add an extra layer of protection, but then just threw it away when i read that open air and ambient light is good for the mycelium.
Doesn't really matter either way.
Quote:
zero123 said: - should i have left them in a dark place and never touch or see them at all for about 2 or 3 weeks, then check them? i guess its possible that just sitting out in the room exposed to all the dust getting kicked up as i walk around each day is not good?
No, leave them out in the open on a shelf so you can track their growth. If you have a proper dry verm layer, then it's unlikely that this contamination was introduced post-inoculation. Don't keep your jars near the floor though.
Top things for better results next time, in order. You may not be able to do all of these, but do as many as you can. 1. Use a pressure cooker 2. Get more than one syringe to minimize chances of a bad syringe wrecking your whole grow 3. Still air box 4. Mask 5. Check that your dry verm layer is adequate. Maybe try making it thicker than you did this time
Here's some good news. You have fine looking mycelium growth in all the jars. This shows that you did the BRF recipe properly, which is something that a lot of new growers mess up. Try again with a new syringe (or, ideally, a couple new syringes.)
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zero123


Registered: 01/03/12
Posts: 22
Last seen: 7 years, 1 month
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Re: Can someone please tell me things are going well? [Re: zero123]
#23781655 - 10/29/16 12:39 AM (7 years, 3 months ago) |
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thanks very much for the feedback! going to do everything suggested and hope for better result next time. its still possible to get a little fruit out of this round, plus there's still a little left in the syringe, but no needle anymore.
the likely culprits of fuckery:
- one of the jars has almost no dry verm layer at all, its all mixed in with the substrate down one side, which presumably happened when they were boiling. when i inoculated the jars, its likely that jar was already contam and spread the mold between 2 other jars.
- i was indeed in a carpeted room with a fan on (but i might have had the foresight to turn it off) and a dog runs around in my room every so often but not when i was in the process. still, being in the bathroom probably would have been better. i'm sure there was all kinds of things floating around during inoculation lmao
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