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InvisibleIndigoAlchemist
Sunflower
Female


Registered: 08/13/16
Posts: 63
Loc: Mind's Eye
Possible contam? 100% colonized and after 8 days of consolidating
    #23779562 - 10/28/16 09:01 AM (7 years, 3 months ago)

I am supposed to begin pasteurization for my substrate today and spawning to bulk tomorrow. I noticed two spots in one jar of B+. The jar is 100% colonized and has been consolidating for 8 or 9 days. I noticed probably 2 or 3 days ago that there are two green spots on the jars. I know that it's not blue, but could this be bruising? The green does not seem to be spreading... Should I just toss this jar? I lost about 50% of my jars after G2G transfers on a newer batch, most likely because of improper grain sterilization procedures. I was going off the gauge instead of the weight. I am at altitude and didn't account for the pressure change, I am having to PC at around 18.5 PSI according the the gauge, the gauge never goes below 3-4 PSI.

Any opinions would be appreciated.



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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Possible contam? 100% colonized and after 8 days of consolidating [Re: IndigoAlchemist]
    #23782275 - 10/29/16 10:30 AM (7 years, 3 months ago)

:shrug: idk wtf that is lol! It's not increasing in size, and is completely surrounded by myc.. I bet you're fine.


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InvisibleIndigoAlchemist
Sunflower
Female


Registered: 08/13/16
Posts: 63
Loc: Mind's Eye
Re: Possible contam? 100% colonized and after 8 days of consolidating [Re: Mad Season]
    #23794474 - 11/02/16 12:33 PM (7 years, 2 months ago)

I would have to assume at the point that it's trich like the rest of my jars from this batch. Maybe it hasn't spread because the jars were so colonized to begin with. The jars that were at 70-80% were taken over more quickly, thats for sure... When I break up the jars, the mycelium recovers but the trich (or what I believe to be) eventually wins and starts killing off the mycelium over a few days. Something went terribly wrong in this G2G I did. I lost 32/38 jars :eek:


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InvisibleIndigoAlchemist
Sunflower
Female


Registered: 08/13/16
Posts: 63
Loc: Mind's Eye
Re: Possible contam? 100% colonized and after 8 days of consolidating [Re: IndigoAlchemist]
    #23800166 - 11/04/16 09:34 AM (7 years, 2 months ago)

So I was looking back at my old pictures I had taken of an agar plate which I used for A2G. I did MS to agar. I obviously overlooked the fact that the plate was contaminated, based on the pacman shape of the plate... Looks to me like I transferred contaminated pieces into the grain jars, unbeknownst to me at the time based on my lack of knowledge. :confused:



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InvisibleMad Season
hookers and blackjack
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Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Possible contam? 100% colonized and after 8 days of consolidating [Re: IndigoAlchemist]
    #23800174 - 11/04/16 09:38 AM (7 years, 2 months ago)

Yeah that is a nasty ass plate lol


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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