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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: HappyCat]
#23789049 - 10/31/16 03:50 PM (7 years, 2 months ago) |
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Thanks everyone for the quick feedback!
Heres todays progress. Doing 24 of these




Edited by xvf (10/31/16 06:16 PM)
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: xvf]
#23789954 - 10/31/16 09:31 PM (7 years, 2 months ago) |
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Inoculated 22 jars (2 got messed up in the PC). Used 10cc's.
And now we wait 
Next time im definitely gonna add food coloring to my agar mix

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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 22 minutes
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Re: [Xvf] First Grow Log [Re: xvf]
#23790363 - 11/01/16 02:54 AM (7 years, 2 months ago) |
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Quote:
xvf said: Thanks everyone for the quick feedback!
Heres todays progress. Doing 24 of these

Did you at least stuff that empty hole in that lid (all) with polyfill tightly?
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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I always make my kids like this
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: [Xvf] First Grow Log [Re: xvf]
#23790658 - 11/01/16 08:30 AM (7 years, 2 months ago) |
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Quote:
xvf said: Inoculated 22 jars (2 got messed up in the PC). Used 10cc's.
And now we wait 
Next time im definitely gonna add food coloring to my agar mix


You inoculated 22 agar plates? Why? You aren't gonna have any to do transfers with! I had one plate inoculated, transferred that to 2 more plates, and will soon be making like 8 transfers from those. That is 8 full plates from a single drop of spore solution. I could do more than that but 8 plates is all I have left prepared.
Also good for you on getting on the agar track before starting. I did PF because I had my heart set on it for 4 years, and immediately wished I had done grains, which basically requires agar before attempting. If you are gonna drop money on a hobby, drop just a little more to do it right!
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Quote:
wtfcrazymofo said:
Quote:
xvf said: Thanks everyone for the quick feedback!
Heres todays progress. Doing 24 of these

Did you at least stuff that empty hole in that lid (all) with polyfill tightly?
I didnt. I was trying to follow the grocery store tek to a T. Guess I gotta cross my fingers and hope it doesnt contam now.
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: MortySmith]
#23790845 - 11/01/16 10:01 AM (7 years, 2 months ago) |
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Quote:
mrknowitall95 said:
Quote:
xvf said: Inoculated 22 jars (2 got messed up in the PC). Used 10cc's.
And now we wait 
Next time im definitely gonna add food coloring to my agar mix


You inoculated 22 agar plates? Why? You aren't gonna have any to do transfers with! I had one plate inoculated, transferred that to 2 more plates, and will soon be making like 8 transfers from those. That is 8 full plates from a single drop of spore solution. I could do more than that but 8 plates is all I have left prepared.
Also good for you on getting on the agar track before starting. I did PF because I had my heart set on it for 4 years, and immediately wished I had done grains, which basically requires agar before attempting. If you are gonna drop money on a hobby, drop just a little more to do it right! 
I'm doing agar simply for the sake of learning. I'm gonna take those 22 agar jars and transfer to ~30-40 WBS jars, let that sit, then spawn to bulk in monotubs. In the future when I get some good genetics I'll have the experience to be able to isloate it.
Edited by xvf (11/01/16 10:02 AM)
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: xvf]
#23790879 - 11/01/16 10:15 AM (7 years, 2 months ago) |
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Should I do something about the agar lids only having tyvek over the hole? Or should I wait it out and see what happens?
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: [Xvf] First Grow Log [Re: xvf]
#23790899 - 11/01/16 10:24 AM (7 years, 2 months ago) |
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Quote:
xvf said: I'm doing agar simply for the sake of learning. I'm gonna take those 22 agar jars and transfer to ~30-40 WBS jars, let that sit, then spawn to bulk in monotubs. In the future when I get some good genetics I'll have the experience to be able to isloate it.
I am not talking about isolation though, I am talking about cleaning. Your mycelium is most likely going to have mold and bacteria growing along with it in your plates. Spore syringes are dirty, my first plates all had some form of contams I think. You will then need to transfer out clean mycelium to another plate and wait for that to colonize to see if its completely clean. Once you have a clean plate, then you can use it to inoculate.
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: MortySmith]
#23790906 - 11/01/16 10:27 AM (7 years, 2 months ago) |
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Quote:
mrknowitall95 said:
Quote:
xvf said: I'm doing agar simply for the sake of learning. I'm gonna take those 22 agar jars and transfer to ~30-40 WBS jars, let that sit, then spawn to bulk in monotubs. In the future when I get some good genetics I'll have the experience to be able to isloate it.
I am not talking about isolation though, I am talking about cleaning. Your mycelium is most likely going to have mold and bacteria growing along with it in your plates. Spore syringes are dirty, my first plates all had some form of contams I think. You will then need to transfer out clean mycelium to another plate and wait for that to colonize to see if its completely clean. Once you have a clean plate, then you can use it to inoculate.
Ahh okay I see what your saying. Damn didnt know spore syringes ran that dirty. I guess I could always buy more jars and transfer whats healthy.
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: [Xvf] First Grow Log [Re: xvf]
#23790955 - 11/01/16 10:49 AM (7 years, 2 months ago) |
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Honestly I wouldn't buy more jars, get glad mini rounds, much cheaper. I don't want to tell you to trash the other plates because agar is expensive, but you are just gonna have more colonies of mycelium growing than you will know what to do with.
Just think how big the colony is once its colonized most of the plate. You only need a tiny little pinhead sized piece to transfer make another full plate. You could easily take 10 transfers from each clean plate, but even with your first transfer from a contaminated plate you could probably find 5 areas to take from. As you do this math, you are gonna realize you should have made less plates lol.
Just to give you an example of what happened with me: I started with just 3 plates, all of different variety cubes (because I thought that was a cool idea lol, wrong). But then I realized I should do multiple transfers from each plate because I could see several strains colonizing the plates at different rates, and I wanted to get a good variety of strains to grow with. What I ended up doing was trashing the 2 most contaminated plates because fuck it, and just made 2 transfers from the one plate I liked the most. My thinking is that once I get a clean plate (which it looks like my 2 transfers are) I can take as many transfers from that as I need to get enough plates to inoculate my jars. Really all you need is 1 clean plate! Well, if you wanna do 40 or so jars, maybe a few clean plates would be better.
Anyways, the whole point of agar, other than strain isolation, is to germinate your spores and clean up your mycelium culture before using for inoculation. You would have been much better off starting with a small handful of plates. Now you are going to have to do 22 transfers, if you want to use every plate, and that is with only 1 transfer per plate!
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SampaJasli
Stranger



Registered: 04/09/07
Posts: 1,396
Loc: Canada
Last seen: 1 year, 8 months
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Re: [Xvf] First Grow Log [Re: MortySmith]
#23791469 - 11/01/16 02:02 PM (7 years, 2 months ago) |
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I must say that I'm following this thread with enjoyment! Thanks for the updates and thanks everybody for the good discussion.
Quote:
mrknowitall95 said: Honestly I wouldn't buy more jars, get glad mini rounds
Those are PC safe?
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Edited by SampaJasli (11/01/16 02:04 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: [Xvf] First Grow Log [Re: SampaJasli] 1
#23791709 - 11/01/16 03:23 PM (7 years, 2 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:36 PM)
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MortySmith



Registered: 03/28/16
Posts: 68
Last seen: 6 years, 4 months
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Re: [Xvf] First Grow Log [Re: amidogen]
#23792084 - 11/01/16 04:59 PM (7 years, 2 months ago) |
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I wouldn't have believed they were either, without reading it in his tek. Seriously such thin plastic, its magic lol.
Edited by MortySmith (11/01/16 04:59 PM)
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 22 minutes
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Re: [Xvf] First Grow Log [Re: xvf]
#23792153 - 11/01/16 05:21 PM (7 years, 2 months ago) |
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The tyvek will work. Not good to re-use though. post future post agar transfer pics!
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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You can just take those that germinate timely/lesser 50% of the termination plates and empty them, refill with agar and make transfers from the best eleven.
I'd buy petris before I got pp5 plastic. I don't think one is easier.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 13 hours, 22 minutes
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I just nocked up 20 petri's in 1/4-1/8 of the time 20 p plates would take( no removing foil and snapping off lids or folding paper towels). It took some more skill, but much less time and more agar square footage . The new hood helped too! I have done pour agar in a sab when I first started way back when. P plates are good to roll with for a beginner IMO. I still love my P plates .
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I use petries and pasty plates. I wouldn't say pasty plates are just for beginners. I really prefer them over petries. Sure pouring plates is slightly faster but but the high walls of pasty plates make them so much easier to handle while keeping your fingers away from the opening. Also I never use paper towels in my pasty plates and I have been reusing the same tin foil for a long time. I can whip up a batch of 20 pasty's in about 48 minutes including the 45 minutes I PC them fore.
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xvf


Registered: 10/21/16
Posts: 59
Loc: Canada
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Re: [Xvf] First Grow Log [Re: MudaFuka]
#23798226 - 11/03/16 04:14 PM (7 years, 2 months ago) |
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Been about 3 days since inoculating agar. Not seeing anything yet (albeit hard to see through the jars). Getting a little fidgety, might do some WBS jars with another syringe I have.
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SampaJasli
Stranger



Registered: 04/09/07
Posts: 1,396
Loc: Canada
Last seen: 1 year, 8 months
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Re: [Xvf] First Grow Log [Re: xvf]
#23798745 - 11/03/16 07:03 PM (7 years, 2 months ago) |
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patience, grasshopper. give it a week or two.
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