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numnum59
Pro-Am Mycologist


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using pf cakes to clean contam'd syringe
#23777581 - 10/27/16 05:35 PM (7 years, 3 months ago) |
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so i have a spore syringe that im having a problem getting clean. kinda what ive gathered is that the BRF cake is more forgiving when it comes to tams, so what if i nocd up a BRF cake to try to get this syringe to germinate mycelium and then xfer it to an agar plate?
thoughts?
when i go Spore syringe to agar i get nothing but trich... (this syringe was from a sponsor, the other ones i got had some bacterial problems but no biggie on agar. not diming them out but just saying i know its gotta have some viable spores in it)
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enlightenment
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Re: using pf cakes to clean contam'd syringe [Re: numnum59] 1
#23777603 - 10/27/16 05:39 PM (7 years, 3 months ago) |
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It should be possible to clean it if you use the agar right. You have to swipe over some spots on your agar dish.
Place some drops of spore liquid on an inoculation loop and try this:

With this method you can get clean sectors transfer them away as fast as possible. (you do not have to do that much swipes. Just do some and it should be enough. Chances are high that you get additional contamination if it takes too long)
Edited by enlightenment (10/27/16 05:49 PM)
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dextr0
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Re: using pf cakes to clean contam'd syringe [Re: enlightenment]
#23777631 - 10/27/16 05:48 PM (7 years, 3 months ago) |
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Enlightenment is there somewhere u can direct me to better explain that method.
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wtfcrazymofo
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Re: using pf cakes to clean contam'd syringe [Re: enlightenment]
#23777632 - 10/27/16 05:48 PM (7 years, 3 months ago) |
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Try to put a drip on the loop (after flame) then touch the agar. I like the spread idea .
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r.lutece
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Re: using pf cakes to clean contam'd syringe [Re: wtfcrazymofo]
#23777648 - 10/27/16 05:52 PM (7 years, 3 months ago) |
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You can also inoculate the agar plate, let it grow a bit, and then pour hot agar on top of the growth. The agar won't slow the mycelium down too much and it can grow upwards through it, but bacteria will have a much harder time recovering. Once the growth comes out the top, scrape some of the clean myc off and transfer it to a new plate.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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numnum59
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Re: using pf cakes to clean contam'd syringe [Re: r.lutece]
#23777736 - 10/27/16 06:11 PM (7 years, 3 months ago) |
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Quote:
r.lutece said: You can also inoculate the agar plate, let it grow a bit, and then pour hot agar on top of the growth. The agar won't slow the mycelium down too much and it can grow upwards through it, but bacteria will have a much harder time recovering. Once the growth comes out the top, scrape some of the clean myc off and transfer it to a new plate.
its not bacteria, but its like they sent me a syringe full of trich
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LogicaL Chaos
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Re: using pf cakes to clean contam'd syringe [Re: numnum59]
#23777749 - 10/27/16 06:14 PM (7 years, 3 months ago) |
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are u touching the agar with the spore syringe needle?
if so, dont do that. just drop a drop of solution on top of agar.
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numnum59
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Re: using pf cakes to clean contam'd syringe [Re: LogicaL Chaos]
#23777766 - 10/27/16 06:20 PM (7 years, 3 months ago) |
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Quote:
LogicaL Chaos said: are u touching the agar with the spore syringe needle?
if so, dont do that. just drop a drop of solution on top of agar.
definitely not,i have about 15 other agar plates from other syringes that are doing just fine. so i know my sterile technique is good. its definitely the syringe, i nocd up a test jar of grains and it went to trich fast as well
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LogicaL Chaos
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Re: using pf cakes to clean contam'd syringe [Re: numnum59]
#23777801 - 10/27/16 06:27 PM (7 years, 3 months ago) |
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ah i see.
I would give the final test and nocc up a PF jar. PF jars seem really resistent to trich in my experience.
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numnum59
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Re: using pf cakes to clean contam'd syringe [Re: LogicaL Chaos]
#23777903 - 10/27/16 07:00 PM (7 years, 3 months ago) |
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Quote:
LogicaL Chaos said: ah i see.
I would give the final test and nocc up a PF jar. PF jars seem really resistent to trich in my experience.
that seems to be the concensus that ive been reading. ill do a write up to maybe shed some light on it
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sinisterminister7
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Re: using pf cakes to clean contam'd syringe [Re: numnum59]
#23777924 - 10/27/16 07:07 PM (7 years, 3 months ago) |
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I'd just toss that shit and move on
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dankington
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Or you can do a warm pour over the spores. Inoculate the plate(s). Make a small batch of agar and when it gets down to the 47ΒΊC or whatever, pour over your inoculations. The mold won't be able to grow through the agar layer, but the mycelium you want can.
You could also try the crack pipe tek
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azur
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Re: using pf cakes to clean contam'd syringe [Re: dankington]
#23778009 - 10/27/16 07:32 PM (7 years, 3 months ago) |
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Sandwiching is for cleaning up bacteria
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dankington
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Re: using pf cakes to clean contam'd syringe [Re: azur]
#23778014 - 10/27/16 07:33 PM (7 years, 3 months ago) |
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That's why I said warm pour. Which is good for mold. no?
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azur
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Re: using pf cakes to clean contam'd syringe [Re: dankington]
#23778062 - 10/27/16 07:42 PM (7 years, 3 months ago) |
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Sandwiching is warm pour. As far as I know, its only purpose is to clean up bacteria. Don't quote me though.
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dankington
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Re: using pf cakes to clean contam'd syringe [Re: azur]
#23778074 - 10/27/16 07:44 PM (7 years, 3 months ago) |
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well, in a sandwich, you cut a slice out of a plate and use that agar over your culture. It's already set, so there's no problem smothering the bacteria. It's fucked.
But if you pour the agar over the plate, you could mix bacteria up with the agar and still fuck your shit up. But mold can't do anything. If you warm pour over the spores, you up your chances IMLE. I know you've grown tons more than me, just sayin'.
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r.lutece
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Re: using pf cakes to clean contam'd syringe [Re: dankington]
#23778110 - 10/27/16 07:50 PM (7 years, 3 months ago) |
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If I remember correctly, the warm pour pseudo-pasteurizes the growth, doesn't it? I could be remembering this wrong. Either way, the bacteria has a much harder time moving through the agar than the myc does. As far as mold spores go, though...not sure.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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azur
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Re: using pf cakes to clean contam'd syringe [Re: dankington]
#23778118 - 10/27/16 07:51 PM (7 years, 3 months ago) |
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Oh. Never heard of that. In my head, if you can successfully cut set agar out of one plate and flip it over into another plate without contamming it, you shouldn't have mold in the first place
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dankington
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Re: using pf cakes to clean contam'd syringe [Re: azur]
#23778141 - 10/27/16 07:55 PM (7 years, 3 months ago) |
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numnum59
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Re: using pf cakes to clean contam'd syringe [Re: dankington]
#23778376 - 10/27/16 08:56 PM (7 years, 3 months ago) |
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Quote:
dankington said: Or you can do a warm pour over the spores. Inoculate the plate(s). Make a small batch of agar and when it gets down to the 47ΒΊC or whatever, pour over your inoculations. The mold won't be able to grow through the agar layer, but the mycelium you want can.
You could also try the crack pipe tek
that crackpipe tek is pretty genius but im getting nothing but straight trich from this syringe. maybe ill try it just to give it a shot, if it doesnt work i have some backup dmt pipes :P
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