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 moment467
Stranger
Registered: 09/22/16
Posts: 209
Last seen: 2 years, 2 months
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 Hello 
#23777073 - 10/27/16 03:02 PM (7 years, 11 months ago) |
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I am reading one of the PF tek's on this site. I am a little confused about spore syringes. Is it smarter to prepare a liquid culture in honey water or to use the spore syringe directly into the brf cakes?
Thanks everyone this site has been a wealth of info.
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 r.lutece
gave Columbia her wings.
Registered: 09/06/15
Posts: 745
Loc: ∅
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Y'know, I don't think I've ever heard of anyone using LC to inoculate cakes, probably because it's a bad idea. LCs are notoriously difficult to detect contams in, and you should really be inoculating the LCs with agar wedges for large bulk grows. Is this your first grow? MS straight into PF cakes is the typical process.
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One goes into an experiment knowing one might fail.
But one does not undertake an experiment knowing one HAS failed.
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:33 PM)
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moment467
Stranger
Registered: 09/22/16
Posts: 209
Last seen: 2 years, 2 months
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Thanks for simplifying it. I want to keep my first time as simple and sterile as possible to provide opportunity for growth. How many grams does each cake produce?
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:33 PM)
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Th3Issu3
Stranger Danger
Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 9 days
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here's a tip too, buy a flow hood or make one.
I went 2 years with out one and now that i have one i don't know why i waited so long.
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:33 PM)
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moment467
Stranger
Registered: 09/22/16
Posts: 209
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Oh wow good for you.
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enlightenment
alchemist
Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 1 year, 5 months
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Of course a flow hood makes things easier but it is not necessary to get successful results. A lot of experienced cultivators around here don't have a flow hood but still show great success.
// edit
wait.
"Oh wow good for you."
... Is this an offense?
People try to help you. Calm down...
Edited by enlightenment (10/27/16 03:41 PM)
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:33 PM)
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moment467
Stranger
Registered: 09/22/16
Posts: 209
Last seen: 2 years, 2 months
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No I meant sincerely that I am glad you have perfected your skills. I hope to become proficient like that someday.
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moment467
Stranger
Registered: 09/22/16
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I should have been more precise in my language. It was meant as a compliment........
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amidogen
see you on the other side
Registered: 05/07/16
Posts: 1,782
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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:34 PM)
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Th3Issu3
Stranger Danger
Registered: 12/30/14
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Last seen: 1 month, 9 days
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Quote:
amidogen said:
Don't ya think a flow hood is a bit of overkill for knocking up PF cakes on your first grow?
yeah for sure, but if OP is anything like us all. he's going to get those first fruits and be addicted.
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moment467
Stranger
Registered: 09/22/16
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Thanks man appreciate it. I am trying to digest as much info as possible.
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moment467
Stranger
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Damn, that is impressive!
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moment467
Stranger
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Posts: 209
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That one one the scale is such a beautiful specimen.
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moment467
Stranger
Registered: 09/22/16
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on*
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Greg
always learning
Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 11 months, 7 days
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Quote:
here's a tip too, buy a flow hood or make one.
I went 2 years with out one and now that i have one i don't know why i waited so long.
A flow hood is extreme overkill unless you're running a mushroom farm or something where you have ridiculous amounts of spawn being made.
A SAB is more than enough.
I maintain over 15 active cultures and many many more gourmet ones with only a SAB.
This is about half of what I have going:
I do plan to start a legit mushroom farm though, so I will build a flow hood eventually.
Also OP, try not to triple post. Condense your writing to a single post at a time.
Otherwise it bumps your thread to the top of the forum constantly and fills pages faster. (Annoying as hell and against the rules.)
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Th3Issu3
Stranger Danger
Registered: 12/30/14
Posts: 671
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Last seen: 1 month, 9 days
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Re: Hello [Re: Greg]
#23777554 - 10/27/16 05:26 PM (7 years, 11 months ago) |
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Maybe, but believe me when I say it makes life so much easier.
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cronicr 
Registered: 08/07/11
Posts: 62,087
Loc: Van Isle 
Last seen: 49 minutes, 5 seconds
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Quote:
amidogen said:
MS syringes are bacterial. Period.
Total crap..they have a tendency but get over it and stop spreading the fear.
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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Hello [Re: cronicr] 1
#23777818 - 10/27/16 06:31 PM (7 years, 11 months ago) |
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MS Syringes are incredibly likely to have some form of contamination.  There.
There's a chance they might not, but overall, a greater chance that they will. This doesn't matter until you're trying to put them to LC, since PF cakes are pretty damn resistant to most of the shit that can ride along in a syringe.
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cronicr
Registered: 08/07/11
Posts: 62,087
Loc: Van Isle
Last seen: 49 minutes, 5 seconds
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Yet we see thousands of success stories with them...
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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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How many fail stories do we see? And what are we talking about specifically here? MS syringe to pf cake, most of that is gonna be success. MS to grain, somewhat less. MS to LC, even less than that. I know you know this, so I'm not sure what exactly is being contested. Are we bringing back syringe noccing?
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dankington
The Stranger
Registered: 03/14/15
Posts: 4,577
Loc: 8te
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MS syringes to grains worked for me for a time, until it didn't anymore. Then I got hit with so much contam, it took forever to start coming back. I'm glad I was able to get a taste for bulk while I was still intimidated by agar work, but agar became a real necessity for me. And, I wish I'd started it earlier, honestly.
I understand where you're coming from, Cron. But you simply cannot say Inoc or Amidogen are wrong. We all know spores have bacteria with them, and this can eventually turn someone off the hobby when they think it's all their fault.
Just a thought.
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cronicr
Registered: 08/07/11
Posts: 62,087
Loc: Van Isle
Last seen: 49 minutes, 5 seconds
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No I would love to see everybody start with agar but to simy say every syringe is dirty is simply garbage fucking info.
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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Inocuole
Scalpel of Evil's Bane
Registered: 11/21/11
Posts: 24,867
Loc: ★
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No I agree. I think plenty of us could plausibly make a clean syringe any time we wanted. But in general I like to discourage entrusting such a task to someone else to do correctly, for the same reason as the pre-made substrates.
Now, I don't have a wide enough sample of cube syringes to definitively say what the percentage is, of course, and I'm sure most people for whom nothing goes wrong probably never post here at all, so there's surely a skewed representation of the actual success rate, meaning we can't use the shroomery as an accurate sample. So the actual truth is that nobody except maybe Workman would have a true-to-reality estimate of those kinds of numbers. That and every situation is unique, so there is no real number. Nothing that happens is ever quite the same as something that happened before it, and that definitely includes microbiology.
But what I do know, is that there are a lot of methods that can work. A fuck ton. But I also know of quite a few can work even better than than those. So, it's like, when I get asked about my past employment in an interview I'm gonna elaborate on the bad ass job. I'll skip talking about Wal Mart. It's experience, sure, but nobody wants to hear about the lowest common denominator. I don't tell my friends about really mediocre places to eat, you know?
That's why I never recommend syringes for anything other than PF tek.
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