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Offlinesavagemessiah115
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Contamination on BRF?
    #23777053 - 10/27/16 02:58 PM (7 years, 3 months ago)

Hello everyone, how are you? I'm here today to see if somebody can help me determine if I have contamination in my mycelium!



Edited by savagemessiah115 (10/27/16 03:03 PM)


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
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Re: Contamination or BRF? [Re: savagemessiah115]
    #23777059 - 10/27/16 02:59 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:31 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? *DELETED* [Re: savagemessiah115]
    #23777062 - 10/27/16 02:59 PM (7 years, 3 months ago)

Post deleted by savagemessiah115

Reason for deletion: Duplicate post



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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777070 - 10/27/16 03:02 PM (7 years, 3 months ago)

I accidentally deleted picture


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Offlineenlightenment
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777076 - 10/27/16 03:03 PM (7 years, 3 months ago)

Bad picture but your BRF jar is contaminated with bacteria.


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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23777079 - 10/27/16 03:04 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:31 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: enlightenment]
    #23777090 - 10/27/16 03:07 PM (7 years, 3 months ago)

What if I use decontaminated precision knives? Mayhaps I could remove the contamination surgically?


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Offlineenlightenment
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777101 - 10/27/16 03:09 PM (7 years, 3 months ago)

:justno:
That's not possible


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777109 - 10/27/16 03:11 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:32 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777119 - 10/27/16 03:13 PM (7 years, 3 months ago)



Previous image was in glove box


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Invisibleamidogen
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Registered: 05/07/16
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777126 - 10/27/16 03:15 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:32 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777132 - 10/27/16 03:16 PM (7 years, 3 months ago)

But what if it's algea?


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777135 - 10/27/16 03:17 PM (7 years, 3 months ago)

I think I'm gonna cry!!!


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Invisibleamidogen
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777159 - 10/27/16 03:23 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:32 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: amidogen]
    #23777358 - 10/27/16 04:20 PM (7 years, 3 months ago)

Ehem! I began by decontamination of a counter top and selected sholderless Mason jars and a crafted glove box, I began with sterilization of jars mixed verm,BRF and water according to tech "someone told me add salt but a day late on his part" so I punctured 4 equally spaced holes with a sterile nail now since I didn't have a pressure cooker I used a canning pot and after 2 hours I removed them to sit over night, my spores arrived the next morning and BRF was immediately inoculated with occasonal needle heating, there are 6 jars by the way and that's the only contaminated specimen which I will refer to as #3 as this was the third jar inoculated,I covered them with sterile aluminium foil and placed them in glove box, tomorrow will be day 7


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777395 - 10/27/16 04:33 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:32 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: amidogen]
    #23777477 - 10/27/16 05:02 PM (7 years, 3 months ago)

I've removed the foil and yes there is a dry layer, the Temp. Fluctuates between 74° and 80° not super stable but rarely above 75°


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23777483 - 10/27/16 05:04 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:32 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: amidogen]
    #23777512 - 10/27/16 05:15 PM (7 years, 3 months ago)

SGFC seems to be the most logical way for a beginner I just wonder if it's to late to restructure my glove box, they are on a shelf with indirect sunlight and occasionally I turn on an overhead florescent for observation purposes!


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23777537 - 10/27/16 05:21 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:33 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: amidogen]
    #23777634 - 10/27/16 05:49 PM (7 years, 3 months ago)

Alright I appreciate the help! So should I only use my glove box for this stage until I switch to SGFC


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23777743 - 10/27/16 06:13 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:35 PM)


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: amidogen]
    #23777807 - 10/27/16 06:30 PM (7 years, 3 months ago)

Just for the mycelium I don't have an incubator cause our walmart caught on fire so I couldn't purchase a tank warmer or anything!


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23777814 - 10/27/16 06:31 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:35 PM)


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Offlineenlightenment
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Re: Contamination on BRF? [Re: savagemessiah115]
    #23777819 - 10/27/16 06:32 PM (7 years, 3 months ago)

You do not need an incubator if your room temp is over 65F.
Even at 60F mycelium would grow.

//edit

too late...


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Offlinesavagemessiah115
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Re: Contamination on BRF? [Re: enlightenment]
    #23777842 - 10/27/16 06:40 PM (7 years, 3 months ago)

Would more pics help?


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Contamination on BRF? [Re: savagemessiah115]
    #23778180 - 10/27/16 08:07 PM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (06/21/18 11:29 AM)


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