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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825164 - 11/12/16 08:09 AM (7 years, 2 months ago) |
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I have colonized some rye jars with oyster mycelium with agar.
I'm not really sure about one jar.
Do you think its contaminated?
Pictrue 1 - Three days after inoculation with an agar wedge. Unfortunately I wasn't able to get the agar wedge sticking to the glas so I was forced to "roll" the jar a bit so that the agar wedge was covered with rye berries. This white stuff on the top could therefore be mycelium which got there because I had to "roll" the jar a bit, so the berries there were inoculated with mycelium. But it also could be cobweb.

Picture 2 - After Picture one I shook the jar. Picture 2 is 3 days after this shaking.

Picture 3 - After the picture I shook it directly.

What do you think guys? Doesn't look good, hm? (don't forget it's oyster mycelium, not Cubensis) 
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825183 - 11/12/16 08:16 AM (7 years, 2 months ago) |
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Top man, well done. Looks perfectly clean and healthy so far. Keep your eyes peeled
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23825185 - 11/12/16 08:19 AM (7 years, 2 months ago) |
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Hey mate,
thank you very much
I wasn't really sure, thought it could be mold or some bacteria. I still have to learn to see the difference between cubensis myc and oyster myc, if it were cubensis, I'd toss it directly
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825216 - 11/12/16 08:33 AM (7 years, 2 months ago) |
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Pretty normal for a fiber (cellulose, lignin and so on) lover, on naked grain (mostly starch).
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23825261 - 11/12/16 08:50 AM (7 years, 2 months ago) |
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Ah okay, just wondered because of this much of condensation which never occured on any other oyster jar before (same Rye). Thought it could be bacterial or somethin
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825266 - 11/12/16 08:53 AM (7 years, 2 months ago) |
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It's clear, so its just the mycelium producing heat, and the water cools on the glass. If it where yellow or any other colour, or even slime, you have issues.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825271 - 11/12/16 08:55 AM (7 years, 2 months ago) |
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Fine, thank you
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23825285 - 11/12/16 09:01 AM (7 years, 2 months ago) |
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No problem, keep up the good work.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23845091 - 11/18/16 12:06 PM (7 years, 2 months ago) |
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Here a picture of my oyster grow in "bags" with straw pellets.
It's going really well, I think next week I can start fruiting them by cutting some cuts into the bags.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23845111 - 11/18/16 12:10 PM (7 years, 2 months ago) |
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One of the best beginners I have seen this year. I am impressed. How are you going fruiting those straw bags?
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23845135 - 11/18/16 12:23 PM (7 years, 2 months ago) |
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Thank you very much, I really like that hobby and because of that I try to do my best for good results
I wait until the mycelium forms the first bigger nodes and than I put these bags in a slightly cooler room. Then I'm going to cut some slots at the points of these nodes, so that the fresh air exchange forces the mycelium to build some fruits there
I hope I can start doing this next week so in about two weeks I'll eat my first oyster pasta 
Regards from Germany!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23845144 - 11/18/16 12:29 PM (7 years, 2 months ago) |
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Those look great! Good job man
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23845145 - 11/18/16 12:30 PM (7 years, 2 months ago) |
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Sounds about right, Good luck
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dreadhed

Registered: 01/21/11
Posts: 175
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23846483 - 11/18/16 08:03 PM (7 years, 2 months ago) |
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If you haven't already you should put some holes in the bags. They need air or they can go anaerobic. You can use a arrow head or knife to put holes in it.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: dreadhed]
#23847421 - 11/19/16 02:34 AM (7 years, 2 months ago) |
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Thank you of course I had already stabbed some some holes with a needle
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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QueenIsabella
Friend



Registered: 10/31/16
Posts: 13
Loc: North America
Last seen: 6 years, 11 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23851773 - 11/20/16 03:06 PM (7 years, 2 months ago) |
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Do you use a flow hood or glove box?
-------------------- I Don't Think So Tim...
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: QueenIsabella]
#23851801 - 11/20/16 03:17 PM (7 years, 2 months ago) |
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No, only a SAB. Search for Spitball Jedis SAB Tutorial, I'm doing it that way. Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23883870 - 12/01/16 08:27 AM (7 years, 1 month ago) |
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Did quite well eventhough I think they were a bit bacterial

-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23886551 - 12/02/16 04:07 AM (7 years, 1 month ago) |
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Just a question, is it possible to have more than one flush with my open air bag method? If yes, how can I initate the second flush?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23886619 - 12/02/16 05:24 AM (7 years, 1 month ago) |
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Cut the fruits off wait 2-4 weeks. Keep hydrated.
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