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AlCapone2k
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Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok?
#23776285 - 10/27/16 10:51 AM (7 years, 3 months ago) |
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hey guys,
besides my cubensis grow experiences I want to grow some oysters
I bought from a laboratory in my city a liquid mycelium culture in a syringe and put some LC on agar to multiply it - don't want to buy a syringe everytime its empty.
The LC in the syringe is definetely sterile, cause it made under laboratory conditions. The needle was also sterilized before first use.
I put some of the LC on an agar plate (LME-Agar) but unfortunately I dropped more LC than I wanted on the plate.
The result is the following:


As you can see the mycelium grew on every spot on the agar. Can you see any contamninations? The mycelium is quite fluffy, but I think this is due to the fact that the humidty inside the agar plate is quite high because of the "to much dropped" LC.
Is it still possible to transfer from this agar plate to other agar plates for multiplying? If yes, which part would you recommend to tranfer from?
Or shall I use it directly for grain inoculation, because tranferring is not necessary/not possible anymore?
Would appreciate any help
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776304 - 10/27/16 10:59 AM (7 years, 3 months ago) |
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Looks ok? I would take and transfer to another plate. The first pic lower left about 7-8 o'clock !looks good for a transfer. Then you can really tell. just take the leading edge.
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Edited by Quadman (10/27/16 11:01 AM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776345 - 10/27/16 11:14 AM (7 years, 3 months ago) |
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You mean this part?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776369 - 10/27/16 11:20 AM (7 years, 3 months ago) |
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Aerial mycelium growing directionally and freely in O2.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776412 - 10/27/16 11:31 AM (7 years, 3 months ago) |
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Sorry, don't understand what you mean, could you explain it?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776464 - 10/27/16 11:46 AM (7 years, 3 months ago) |
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Sorry, if you look at your image and compare, the long bits. Where you noted, but also either side of it.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776489 - 10/27/16 11:52 AM (7 years, 3 months ago) |
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So you mean this long bits are aerial myelium? Is that good or bad?
Didn't unterstand it yet, maybe it's my english, I'm from germany
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776498 - 10/27/16 11:55 AM (7 years, 3 months ago) |
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It's good in this case, it means it has strong growing genetics. Far too thick and radial to be bacterial avoidance.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776507 - 10/27/16 11:56 AM (7 years, 3 months ago) |
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Ah, ok, fine, sorry for my stupid understanding
So the triangle I drawed on the picture is good for transfer?
Or do you think I should do both:
Transfer the triangle to another agar plate and also take a wedge and directly inoculate some grain jars?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776515 - 10/27/16 12:00 PM (7 years, 3 months ago) |
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Have you checked for bacteria pools or unusual colours in the agar? If you have, and to me it looks healthy, transfer away.
Just keep checking for anything odd.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Ferather]
#23776560 - 10/27/16 12:22 PM (7 years, 3 months ago) |
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Actually I also can't see anything looking strange on the plate and the free of mycelium agar spots also look well.
"Transfer away" on agar (for further cleaning) or also to spawn, what do you recommend?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776603 - 10/27/16 12:31 PM (7 years, 3 months ago) |
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In that case I would do both, the agar transfer will be backup. Good job, I hope you get some strong genetics.
Good luck, Ferather
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776621 - 10/27/16 12:38 PM (7 years, 3 months ago) |
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Thank you Ferather for your help and tribute :P
I'll update here after the next check-point.
Regards from Germany!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
Last seen: 1 year, 3 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776731 - 10/27/16 01:16 PM (7 years, 3 months ago) |
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Yes transfer the triangle. It looks like good plates ,but that triangle is more normal mycelium growth. It should continue looking like that after transfer. Keep the plates and wait and see if anything changes.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Quadman]
#23776758 - 10/27/16 01:25 PM (7 years, 3 months ago) |
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"more normal mycelium growth" = good growth?
So: 1. Transfer Triangle 2. See what happens on new plate 3. Let the first plate colonize further and drop wedges to rye spawn.
Correct?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
Last seen: 1 year, 3 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23776770 - 10/27/16 01:28 PM (7 years, 3 months ago) |
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That would be my plan those two plates are probably usable give them some time . The new transfer should be good for sure , time will tell.
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Edited by Quadman (10/27/16 01:30 PM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Quadman]
#23776804 - 10/27/16 01:40 PM (7 years, 3 months ago) |
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It's the same plate but two pictures 
But thank you, I let it grow another day until dropping it to grain I think. The plate hasn't be fully colobized for rye drop, right?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
Last seen: 1 year, 3 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23777032 - 10/27/16 02:54 PM (7 years, 3 months ago) |
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You can drop on grain anytime. If there should be some mold there you just waste some grain.
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Edited by Quadman (10/27/16 02:54 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: Quadman]
#23777107 - 10/27/16 03:10 PM (7 years, 3 months ago) |
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Just do both, one to grain, one to agar. Leave the original to grow. If it where dirty the longer you leave it the worse it gets.
However, this is scaremongering. It looks clean. He checked for usual signs of bacteria.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Pleurotus ostreatus (Oyster) Grow --> Agarplate contaminated or ok? [Re: AlCapone2k]
#23777197 - 10/27/16 03:34 PM (7 years, 3 months ago) |
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Yes, acutally I can't see any contamn, and I know how bacteria and mold look on an agar plate, since I hade these issues before(eventhough all in all my no-contamn rate is thank god quite good).
So tomorrow I'll do the transfer for multiplication and the agar wedge drop to grain. I think I will also make a second rye jar to inoculate directly from the mycelium syringe of the laboratory. Just to see, if it's also possible without contamn.
Maybe I loose some rye with it, but rye is the less expensive thing in this hobby so I think I can afford it :P
Thank you again for all your help.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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