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OfflineEdmunter
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Re: Mexicana grow [Re: cronicr]
    #23773524 - 10/26/16 02:40 PM (7 years, 4 months ago)

What would you suggest for Psy Mex?

My 'WEDGES' are a bit fluffy on some and I thought it might need something else.

WEDGES = Chunks of culture I cut out in the shape of a wedge.

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InvisibleMycolorado
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Re: Mexicana grow [Re: cronicr]
    #23773544 - 10/26/16 02:51 PM (7 years, 4 months ago)

Quote:

cronicr said:
Well now he is taking a break:hehehe: back on topic people..munter try switch your media up it helps with them.




What's this supposed to mean?  Did C10 really just get banned for this shit?  If so, your "Moderator" status and or qualifications are highly suspect.

The OP was using jargon incorrectly or at the very least using terms in a way that aren't commonly use around here.  Was C10 a bit abrasive?  Maybe.  Was he out of line and deserving to be banned?  No Fucking Way.


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OfflineEdmunter
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Re: Mexicana grow [Re: Mycolorado]
    #23773554 - 10/26/16 02:54 PM (7 years, 4 months ago)

Can somebody tell me what the fuk I said that was so mind bendingly confusing?

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OfflinecronicrFacebook
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Re: Mexicana grow [Re: Edmunter]
    #23773562 - 10/26/16 02:58 PM (7 years, 4 months ago)

Quote:

Edmunter said:
What would you suggest for Psy Mex?

My 'WEDGES' are a bit fluffy on some and I thought it might need something else.

WEDGES = Chunks of culture I cut out in the shape of a wedge.



Any type of a switch up will do.


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OfflineEdmunter
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Re: Mexicana grow [Re: cronicr]
    #23773606 - 10/26/16 03:10 PM (7 years, 4 months ago)

Is rye water/agar any good for these?

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Offlinetombosley8
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Re: Mexicana grow [Re: Mycolorado]
    #23773613 - 10/26/16 03:12 PM (7 years, 4 months ago)

what munter said sound fine to me.

maybe he could have said a culture grown from a wedge cut from another plate of cultured agar but that's a lot more than needed. we all know what growing from wedges means right?

and I think we knew what he meant when he said he is growing out wedges.

Meaning he didn't noc the plate directly with spores, clones, etc.
Meaning he did noc the plate with a wedge from another agar plate.

good enough for me:shrug:

Either c10 was confused or he just wanted to argue about something that was maybe not perfectly grammatically correct

Sure I understand jargon needs to be held to the definition but I truly think munt was using it in close enough to the right context.

Now that I had to rant and go off topic to explain I will say I usually use swabs to noc agar with spores and haven't had much problems after a few transfers.

Maybe loops can help with spreading the spores away from contam and getting single colonies but I've never tried and didn't think that was so necessary...  I have tried with my scalpel a couple times and just seemed to get less growth due to less spores but the colonies were still all mixed together.

Either way you can still get a good organized clean culture with a few transfers.


so back to the point.  Cron is right on switching up the nutes for your agar. It helps a lot.

Dog food agar is super cheap and easy just as easy as MEA just isn't as clear but that shouldn't matter anyway.

Just grind up dog food in a coffee grinder or blender and use in place of malt extract.

I use slightly less nutes in my agar when making transfers for faster more rhizo growth. but when transfering to grain, lc, etc you want the regular nute level for strongest and fastest leap off. And spore germination should be the normal recipe too.

so recipe normally is 10g dog food  10g agar  500ml water

I use 8g dog food 10g agar 500ml water

start a few cultures of the same print so you can choose the strongest cleanest from them and discard the questionable ones.

Edited by tombosley8 (10/26/16 03:21 PM)

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InvisibleMycolorado
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Re: Mexicana grow [Re: cronicr]
    #23773644 - 10/26/16 03:20 PM (7 years, 4 months ago)

So this is how it works around here?  You didn't ask anyone to stay on topic anywhere.  C10 answered his question and gave him some additional and helpful info.  And when I ask what's up, you send me this:


Get off your power trip.

Back on topic so I don't get banned too...a lower nute content might prove beneficial in producing more aggressive mycelial growth.


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OfflineEdmunter
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Re: Mexicana grow [Re: tombosley8]
    #23773681 - 10/26/16 03:31 PM (7 years, 4 months ago)

Quote:

tombosley8 said:
what munter said sound fine to me.

maybe he could have said a culture grown from a wedge cut from another plate of cultured agar but that's a lot more than needed. we all know what growing from wedges means right?

and I think we knew what he meant when he said he is growing out wedges.

Meaning he didn't noc the plate directly with spores, clones, etc.
Meaning he did noc the plate with a wedge from another agar plate.

good enough for me:shrug:

Either c10 was confused or he just wanted to argue about something that was maybe not perfectly grammatically correct

Sure I understand jargon needs to be held to the definition but I truly think munt was using it in close enough to the right context.

Now that I had to rant and go off topic to explain I will say I usually use swabs to noc agar with spores and haven't had much problems after a few transfers.

Maybe loops can help with spreading the spores away from contam and getting single colonies but I've never tried and didn't think that was so necessary...  I have tried with my scalpel a couple times and just seemed to get less growth due to less spores but the colonies were still all mixed together.

Either way you can still get a good organized clean culture with a few transfers.


so back to the point.  Cron is right on switching up the nutes for your agar. It helps a lot.

Dog food agar is super cheap and easy just as easy as MEA just isn't as clear but that shouldn't matter anyway.

Just grind up dog food in a coffee grinder or blender and use in place of malt extract.

I use slightly less nutes in my agar when making transfers for faster more rhizo growth. but when transfering to grain, lc, etc you want the regular nute level for strongest and fastest leap off. And spore germination should be the normal recipe too.

so recipe normally is 10g dog food  10g agar  500ml water

I use 8g dog food 10g agar 500ml water

start a few cultures of the same print so you can choose the strongest cleanest from them and discard the questionable ones.





Thanks

And hallelujah I thought I was going mad with my posts and speaking a foreign language.

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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23773762 - 10/26/16 03:57 PM (7 years, 4 months ago)

I just thought id let you all know that me and C10 have ironed out our differences on PM and reccon we will laugh about this on our honeymoon.

Everyone has a bad day and I think we had ours together.

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Invisibledankington
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Re: Mexicana grow [Re: Edmunter]
    #23774098 - 10/26/16 05:39 PM (7 years, 4 months ago)

:hug:

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OfflineEdmunter
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Re: Mexicana grow [Re: tombosley8]
    #23775552 - 10/27/16 03:21 AM (7 years, 4 months ago)

It seems worth making a few plates.  I have some dog food but havent checked it for nute values.  what do you look for in your doggy bits?

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OfflineLogicaL ChaosM
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Re: Mexicana grow [Re: Edmunter]
    #23775570 - 10/27/16 03:52 AM (7 years, 4 months ago)

yikes. glad u guys hugged it out, damn.

Im just here for the mexicana pics :popcorn:


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Offlinetombosley8
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Re: Mexicana grow [Re: LogicaL Chaos]
    #23776426 - 10/27/16 11:37 AM (7 years, 4 months ago)

I think most basic dog foods will work.

I just look for the cheapest stuff but others have told me even grain free high end stuff works great too.

Blinding Leaf said he uses purina the original flavor green bag with good results and I've tried a few different generic brands and they all seem to work the same.

I think corn is usually the first ingredient.


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Re: Mexicana grow [Re: tombosley8]
    #23776430 - 10/27/16 11:38 AM (7 years, 4 months ago)

I would be surprised if there was a dog food that didn't work.

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Re: Mexicana grow [Re: bodhisatta]
    #23776469 - 10/27/16 11:47 AM (7 years, 4 months ago)

So with this Psilocybe Mexican I am going to take a wedge or 3 from the slow, clean growing culture and put it on my new media which is dog food and agar 8:10 gram in 500 ml of water.

I have already put a wedge in a small jar of Rye and hope for movement soon.

So how do I shift this media around and why..........e.g. trying to germinate, cloning ect

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Re: Mexicana grow [Re: Edmunter]
    #23776558 - 10/27/16 12:22 PM (7 years, 4 months ago)

You could change back and forth every transfer if you want. I only do about every 2 transfers without any noticeable effects.

Usually spore germination you want slightly softer agar and the full nutrient mix so 10g dog food and slightly less agar so like 9-9.5g agar per 500ml should work depending on your agar.

for transferring wedges to new plates and if that plate is also going to be transferred to another plate then you want slightly less nutes with the regular agar level of 10g. As said 8g of nutes per 500ml water is about as low as you want.

When you plan on inoculating lc, grain, etc with a plate you want to have the full nutrition and agar for strong fast leap off. So 10g agar 10g dog food 500 ml water.


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OfflineEdmunter
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Re: Mexicana grow [Re: tombosley8]
    #23776579 - 10/27/16 12:26 PM (7 years, 4 months ago)

Make sense, thanks.

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Re: Mexicana grow [Re: Edmunter]
    #23776866 - 10/27/16 02:04 PM (7 years, 4 months ago)

Quote:

Edmunter said:
Can somebody tell me what the fuk I said that was so mind bendingly confusing?



Nothing was particularly confusing, you have to understand the sometimes bizarre mentality of some people, they pretend to be fucking ignorant cunts in a weird attempt to appear "smart", but more often than not this backfires very badly, as is the case here, they either appear as stupid or just as dicks to those who know they are faking it. Use the ignore function, it's a great feature, if someone is such a cunt that they are pretending to be that stupid you will have a better time on the forum by not seeing their future disingenuous posts, but if the genuinely are that ignorant & stupid you are also better off, so it's really win-win.

I think RR advised on gypsum as an agar additive for stones, along with the coffee already advised.

Edited by blackout (10/27/16 02:09 PM)

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OfflineEdmunter
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Re: Mexicana grow [Re: blackout]
    #23777222 - 10/27/16 03:42 PM (7 years, 4 months ago)

Ive now made up with C10, he genuinely said he really didn't understand what I was saying.  He sounded intelligent and like someone who should get it and I thought he was trolling me or nip picking.  He say different and if thats the case then maybe he was confused and not as  open with wording as the rest of us and wanted to correct my terminology.

There's no hard feelings and id like to get on with growing stones now.  Id like to point out that some of this is not for noobs and my language may not be text book as I like it to be a bit more forum chatty banter rather than strict classroom.


So have a bunch of mushrooms as a peace sign and lets rock!

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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23779751 - 10/28/16 10:59 AM (7 years, 4 months ago)

Ok Im taking one of the good looking Meicana and Sandose cultures and Im going to transfer them to a 8:10 dog food agar ratio plates.  Give their legs a good run and let em loose hoping to transfer back to 10:10 for a few tiger drops into some grains to get the G2G going.  In the mean time I am going to make some LI s so I can pp5 a few so I can get some spore prints to give away to anyone who needs them.

Anything sound wrong there?

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