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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
help me decide what to do
    #23775393 - 10/27/16 01:05 AM (8 years, 2 months ago)

ok guys so i have a wbs jar thats colonizing with only about 3 innoc points ( i didn't tiger drop 2.0  :facepalm3: ) all but one of my colonized plates of agar are contam free. I have 5 transfers colonizing but dont have enough growth. I want to be on the same page with the colinization times of my two wbs jars, so i can make a 20qt mini mono hybrid.

so everyone what should I do?
(a.) tiger drop 2.0 into my wbs quart. colonize spawn to bulk and clone (b.) make more tranfers out of my plate.

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Offlinetump
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Registered: 03/17/16
Posts: 2,383
Last seen: 7 years, 10 months
Re: help me decide what to do [Re: Hallucyjunkie99]
    #23775414 - 10/27/16 01:20 AM (8 years, 2 months ago)

How about just going with the two jars of spawn for mini mono. Or if speed is a hurry make li for a plate and knock up more jars.

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: help me decide what to do [Re: tump]
    #23775421 - 10/27/16 01:26 AM (8 years, 2 months ago)

guess im going to noc up the second wbs jar i have sterilized and make more pasty plates once i have a fruit to clone :bigblunt: I was thinking about using an LI but too many contam vectors come into play. Maybe when i do a 66qt tub

Edited by Hallucyjunkie99 (10/27/16 01:35 AM)

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Offlinetump
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Registered: 03/17/16
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Re: help me decide what to do [Re: Hallucyjunkie99]
    #23775605 - 10/27/16 04:34 AM (8 years, 2 months ago)

Dude with clean li and then g2g you can have ten 66 tubs. I believe in you

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: help me decide what to do [Re: tump]
    #23775621 - 10/27/16 04:53 AM (8 years, 2 months ago)

Thanks tump hopefully I get there one day:wink:. For now I  just need to get a few bulk grows under my belt:evil:

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OfflineLogicaL ChaosM
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Re: help me decide what to do [Re: Hallucyjunkie99]
    #23775755 - 10/27/16 07:08 AM (8 years, 2 months ago)

Do a bunch of grain to grain transfers :shrug:

Or cut your infection-free cultured agar up into wedges that will fit in 20 quarts of WBS, and do it all at the same time.


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InvisiblePirateSwazey
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Re: help me decide what to do [Re: LogicaL Chaos] * 1
    #23775855 - 10/27/16 08:07 AM (8 years, 2 months ago)

Put your plates that are ready in the fridge until the others catch up. Now you can inoculate all your jars at the same time.


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