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Offlinedarqism
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Liquid honey culture
    #23775351 - 10/27/16 12:40 AM (7 years, 3 months ago)

Hi everyone,
It's my first post on sroomery :smile: I'm trying to make a liquid honey culture. My substrate is a rye grain so to make lc I used a soaking water from rye.
200ml soaking water
8ml honey

First I sterilized only the soaking water in pc (15psi) for half an hour then I mixed the soaking water with honey and sterilized for 20min more.

This is how look my jar.







Are those floating flakes fine? 
thanks :smile:


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Invisibleazur
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Re: Liquid honey culture [Re: darqism]
    #23775357 - 10/27/16 12:42 AM (7 years, 3 months ago)

Not good


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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Invisibleirequirechocolate
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Re: Liquid honey culture [Re: darqism]
    #23775371 - 10/27/16 12:50 AM (7 years, 3 months ago)

Those 'flakes' could be caramelized sugars or even proteins from the boil/sterilization. BUT, I've done a few experiments in my lab and there are also definitely organisms that flocculate into similar chunks. It's hard to tell from the pictures, and considering that honey is unusually good at keeping bacteria at bay.
I'v never tried to make honey LC for mushrooms or anything, but I do work w/ microbes, so take anything I say w/ a grain.


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Offlinedarqism
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Re: Liquid honey culture [Re: irequirechocolate]
    #23775546 - 10/27/16 03:02 AM (7 years, 3 months ago)

I think it's not caramelized sugars or protein because I noticed those chunks after a few days and it's look like it's still growing up :P

thanks for you help :smile:


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Offlinetump
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Re: Liquid honey culture [Re: darqism]
    #23775626 - 10/27/16 04:58 AM (7 years, 3 months ago)

Too much honey. Not good. Straight grain water lc does just fine, honey is the worst at making a jar clear and clean. How are you knocking up such a lc. If you start form spore syringe you might be out of luck. If your not starting out with agar work id start with that. In the archives there the poor mans agar tek for those who can't find agar easy. That will start you off until you can find agar and give you growth to cut out when do fine it.


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InvisibleHallucyjunkie99

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Re: Liquid honey culture [Re: tump]
    #23775632 - 10/27/16 05:06 AM (7 years, 3 months ago)

Even better pastys agar tek down in my sig op:stoned:


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InvisiblePastywhyteMDiscord
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Re: Liquid honey culture [Re: Hallucyjunkie99]
    #23775758 - 10/27/16 07:10 AM (7 years, 3 months ago)

That shit is all bacteria. Dump that.


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Offlinedarqism
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Re: Liquid honey culture [Re: Hallucyjunkie99]
    #23775810 - 10/27/16 07:42 AM (7 years, 3 months ago)

I was following this tek
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=10446142&PHPSESSID=&fpart=1#10446142

Quote:

4 ml of honey per 100 ml of water (around 4 % honey solution).




so I though 8ml of honey will be fine

Quote:

If you start form spore syringe you might be out of luck.



I used a spore print and I was doing everything in my glove box. I'll try one more time with destiled water.

About agar. How should I store agar after injection?


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Offlinetump
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Re: Liquid honey culture [Re: darqism]
    #23780613 - 10/28/16 04:42 PM (7 years, 3 months ago)

Store it in the box


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OfflineGreg
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Re: Liquid honey culture [Re: tump]
    #23780628 - 10/28/16 04:46 PM (7 years, 3 months ago)

Spore print or syringe doesn't matter, unfortunately either has high chances of contamination if taken directly to LC.

Also you're going to want to use a SAB instead of a glovebox:
https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771


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Offlinetump
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Re: Liquid honey culture [Re: Greg]
    #23781697 - 10/29/16 01:27 AM (7 years, 3 months ago)

Now if you really want to use straight spores form a print you could always make it into a syringe and then divide with a high of sterile water like 500 ml of water. It reduces the chance of a contam spores with still a fair amount of cubes spores . It's a lot of work for 97 percent success rate.
Less work is to agar plates and use a sterile swab to dab in the middle form the spore print. Cut away the clean grow into new agar plates.


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Offlinedarqism
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Re: Liquid honey culture [Re: tump]
    #23787709 - 10/31/16 07:41 AM (7 years, 2 months ago)

thanks a lot guys for your advises :thumbup:

I'll make a SAB and try then. :smile:


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