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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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How long until contams show on Agar?
#23774626 - 10/26/16 08:18 PM (7 years, 3 months ago) |
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Hey. I poured my first three Agar plates ever, but it was extremely fucking hard. I mean, my PC is tiny so I had to use short wide mouth mason jars, which made it unbelievably hard to pour. Secondly, my SAB got all condensation-y from the Lysol, and I couldn't see shit. I couldn't cut open the sleeve of petri dishes inside the SAB because the scissors were shit and the material was tough. It took me forever. I accidentally opened one of the petris when trying to get that shit out of the sleeve. I spilled a lot of agar everywhere, including on the outside of the petris. I couldn't wrap the friggen cling wrap on the petris without shaking it around and getting agar all over the inside. I just fucked up big time. I don't know how you guys say its easy.
Anyway, the point is, how long until contams start showing up? I dont wanna use them until I know for sure they're not contam'd.
And you guys make agar seem super easy, even for noobs. Did you guys struggle in the beginning too? I feel like a retard
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23774654 - 10/26/16 08:27 PM (7 years, 3 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:30 PM)
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: How long until contams show on Agar? [Re: amidogen]
#23774671 - 10/26/16 08:33 PM (7 years, 3 months ago) |
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Quote:
amidogen said: Hell yeah I struggled. I do no pour agar ala Pastywhyte, but the hardest thing for me was not having my hands over open media and successfully transferring myc without it getting stuck to my blade. Practice makes perfect. Something like 80 plates of agar later and I feel pretty good about it.
And mold usually germinates for me within 3-5 days. Keep fighting the good fight, it'll pay off 
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23774678 - 10/26/16 08:36 PM (7 years, 3 months ago) |
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You wrap after the agar solidifies, the mental image of you wrapping plates while the agar is still liquid is cracking me the fuck up. I only wrap after I inoculate the plate or transfer from it, nothing else. I just leave them in stacks in the SAB until they're used up. (sometimes up to a week)
Wait longer after spraying the lysol before working. You really don't need lysol though, just plain water + soap or even just water.
Opening a petri accidentally for a moment probably wont cause any issues, I've done that before.
Spilling agar is a problem but it's probably not going to ruin everything. Although, if there is a bridge of agar from the outside of the plate to the inside, contams can ride that all the way in to the dish. If the agar is really scrambled inside the dish, you may just want to start with new dishes and move them as little as possible after the agar is poured.
Other than that, get sharper scissors and a bigger PC. 
About the contams, leave the plates in the SAB for a day or two if you're really concerned. Bacteria shows up really quick, mold can take a few days or more though...
For all the shitty pours I've done (300+ plates) I've only had three plates contam before I used them. All of those contams were single bacteria colonies.
I spilled agar the first three times I poured, never since then unless I'm drunk lol.
Edited by Greg (10/26/16 08:46 PM)
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23774692 - 10/26/16 08:39 PM (7 years, 3 months ago) |
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Quote:
Plazmotech said:
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
Im new to agar as well, but im not sure about this statement. Ive done both ways... Pour and no pour and honestly they both are teaching me quite alot of info. Learning when to spot bacteria and contams is the big thing, also which or what sectors to take the sample from.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: How long until contams show on Agar? [Re: numnum59]
#23774697 - 10/26/16 08:40 PM (7 years, 3 months ago) |
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 That too, there is nothing wrong with no-pour if you'd rather do that.
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Moabfighter
Tam Fighter


Registered: 12/13/15
Posts: 2,710
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Re: How long until contams show on Agar? [Re: Greg]
#23774723 - 10/26/16 08:47 PM (7 years, 3 months ago) |
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So what if I've got absolutely nothing in over a week on some plates....? No mold. But no myc either......
-------------------- KSSS And PE WBS.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: How long until contams show on Agar? [Re: Moabfighter]
#23774736 - 10/26/16 08:50 PM (7 years, 3 months ago) |
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Either you need to wait longer or your spores or whatever was bad. You could try softer agar I suppose. Some people say that helps with germination.
I've only ever had spores fail to germinate twice (without contams) out of many successful attempts. (PEU syringe, PE swab)
Edited by Greg (10/26/16 09:02 PM)
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: How long until contams show on Agar? [Re: Greg]
#23774770 - 10/26/16 08:59 PM (7 years, 3 months ago) |
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Quote:
Greg said: You wrap after the agar solidifies, the mental image of you wrapping plates while the agar is still liquid is cracking me the fuck up. I only wrap after I inoculate the plate or transfer from it, nothing else. I just leave them in stacks in the SAB until they're used up. (sometimes up to a week)
Fuck! I knew something was fucked up. The whole time I was doing it I was thinking "I dont think this is physically possible"
Quote:
Greg said: Spilling agar is a problem but it's probably not going to ruin everything. Although, if there is a bridge of agar from the outside of the plate to the inside, contams can ride that all the way in to the dish. If the agar is really scrambled inside the dish, you may just want to start with new dishes and move them as little as possible after the agar is poured.
Shiit okay that gives me a sliver of hope…
Quote:
Greg said: For all the shitty pours I've done (300+ plates) I've only had three plates contam before I used them. All of those contams were single bacteria colonies.
Yep but I don't think you understand how much of a failure this pour was. I dont think i've ever prepared for a task so much and then failed so hard in my life.
Thanks for your insight!
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23774774 - 10/26/16 09:00 PM (7 years, 3 months ago) |
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No problem and good luck with your agar work in the future!
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: How long until contams show on Agar? [Re: numnum59]
#23774775 - 10/26/16 09:00 PM (7 years, 3 months ago) |
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Quote:
numnum59 said:
Quote:
Plazmotech said:
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
Im new to agar as well, but im not sure about this statement. Ive done both ways... Pour and no pour and honestly they both are teaching me quite alot of info. Learning when to spot bacteria and contams is the big thing, also which or what sectors to take the sample from.
Yeah but to be honest it's also kind of an ego thing, lol… I'll feel a lot more accomplished if I can do this shit on a petri dish like a scientist lmao
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How long until contams show on Agar? [Re: Plazmotech] 1
#23774790 - 10/26/16 09:07 PM (7 years, 3 months ago) |
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last link in signature, first one you find there,
ideally you want to be confident in your agar work.
sometimes there can be contamination on a dish that will not grow either because the media is wrong or the temperature or something else. but it may grow later on if you take it with you and put it in your spawn.
if you see contamination on agar you get a feel for your contamination rate. you want to try to get that as low as possible. you can do it in a 66Q SAB pouring 20 plates in two stacks of 10. trust me I've done it. you don't need super fancy shit at all. but just know that sometimes you don't see contamination, so you must rely on your skill to get clean plates. also if any single contamination shows up on a plate that means that anything else could potentially be on that dish, so it's best to make a transfer to a new dish then transfer again as soon as enough growth is there to transfer. thus leaving behind potential rogue contaminants
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23774807 - 10/26/16 09:12 PM (7 years, 3 months ago) |
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Quote:
Plazmotech said:
Quote:
numnum59 said:
Quote:
Plazmotech said:
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
Im new to agar as well, but im not sure about this statement. Ive done both ways... Pour and no pour and honestly they both are teaching me quite alot of info. Learning when to spot bacteria and contams is the big thing, also which or what sectors to take the sample from.
Yeah but to be honest it's also kind of an ego thing, lol… I'll feel a lot more accomplished if I can do this shit on a petri dish like a scientist lmao
RRs videos do a good job of explaining agar...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: How long until contams show on Agar? [Re: numnum59]
#23774817 - 10/26/16 09:14 PM (7 years, 3 months ago) |
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: How long until contams show on Agar? [Re: bodhisatta]
#23774823 - 10/26/16 09:17 PM (7 years, 3 months ago) |
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Quote:
bodhisatta said: https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954
Thanks! But I've actually already read your tek. It's more the practice that's fucking me… I feel like I'm not coordinated enough to do everything properly lol
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23829677 - 11/13/16 04:03 PM (7 years, 2 months ago) |
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Noob solidarity  I feel your pain Plazmotech. I've been pouring my first plates in a SAB this month. I've pour agar on my fingers and even burned the tip of my gloves doing my first transfer. This weekend, I was proud of myself that I did it all perfect... and then I dropped the very last plate while I was trying to wrap it lol. It's currently wrapped and set aside to see if contam grows on it, but it's been 3 days and still nothing.
--------------------
My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Re: How long until contams show on Agar? [Re: flyontoast]
#23830535 - 11/13/16 08:58 PM (7 years, 2 months ago) |
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Quote:
flyontoast said: Noob solidarity  I feel your pain Plazmotech. I've been pouring my first plates in a SAB this month. I've pour agar on my fingers and even burned the tip of my gloves doing my first transfer. This weekend, I was proud of myself that I did it all perfect... and then I dropped the very last plate while I was trying to wrap it lol. It's currently wrapped and set aside to see if contam grows on it, but it's been 3 days and still nothing.
Haha yeah I think I got the hang of it this weekend! Last pour was near perfect. But now I'm out of plates… Ill have to order more on amazon
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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A.RichardTrickle
Feel like a Stranger

Registered: 11/04/16
Posts: 808
Loc: Silver Mine Peggy Sue
Last seen: 7 years, 1 month
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23838456 - 11/16/16 11:10 AM (7 years, 2 months ago) |
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Quote:
Plazmotech said:
Quote:
amidogen said: Hell yeah I struggled. I do no pour agar ala Pastywhyte, but the hardest thing for me was not having my hands over open media and successfully transferring myc without it getting stuck to my blade. Practice makes perfect. Something like 80 plates of agar later and I feel pretty good about it.
And mold usually germinates for me within 3-5 days. Keep fighting the good fight, it'll pay off 
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
O pour agar IS right my friend. It is as effective as petri poured agar if used properly, with the right gear. Judging by what you stated I'd say you would do better to try the no pour tek until you have a bigger PC that will fit a lab flask or double deuce glass beer bottle. Petris are great, I used to play with them at my mentors lab, but without proper gear you are more likely to fail. From me to you, as I know you are a serious cultivator, I'd try no pour to get some clean cultures. Best wishes either way man. Dick
-------------------- "When eating shit, it is best not to nibble. Bite, Chew. Swallow. Repeat." "If you're making love to your old lady, someone else is fucking her" "Douchebags are children who never grew up, like Sheeklette, we should pity them." [quote]Niffla said: [quote]A.RichardTrickle said: Dick[/quote] http://www.youtube.com/v/kbwNUOUy-3c[/quote]
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Plazmotech
we love the dead



Registered: 10/11/16
Posts: 353
Loc: earthless
Last seen: 2 years, 1 month
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Quote:
A.RichardTrickle said:
Quote:
Plazmotech said:
Quote:
amidogen said: Hell yeah I struggled. I do no pour agar ala Pastywhyte, but the hardest thing for me was not having my hands over open media and successfully transferring myc without it getting stuck to my blade. Practice makes perfect. Something like 80 plates of agar later and I feel pretty good about it.
And mold usually germinates for me within 3-5 days. Keep fighting the good fight, it'll pay off 
Thanks man. I don't want to learn no pour, cause if I'm gunna learn it, I wanna learn it right. How long does Myc take to grow? Around the same time?
O pour agar IS right my friend. It is as effective as petri poured agar if used properly, with the right gear. Judging by what you stated I'd say you would do better to try the no pour tek until you have a bigger PC that will fit a lab flask or double deuce glass beer bottle. Petris are great, I used to play with them at my mentors lab, but without proper gear you are more likely to fail. From me to you, as I know you are a serious cultivator, I'd try no pour to get some clean cultures. Best wishes either way man. Dick
Thanks Dick, but it's been 20 days since that post. In that time, I made 8 pasty plates, and bought a 22 quart PC, and heated and poured 10 regular agar plates, and inoculated some grain jars
-------------------- When a man lies, he murders some part of the world. These are the pale deaths which men miscall there lives. All this I cannot bear to witness any longer. Cannot the kingdom of salvation take me home? Plazmotech's Kratom Grow
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lazerus00
Stranger

Registered: 08/28/16
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Re: How long until contams show on Agar? [Re: Plazmotech]
#23838799 - 11/16/16 01:14 PM (7 years, 2 months ago) |
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Glad to see everything worked out!
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