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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23774577 - 10/26/16 08:06 PM (7 years, 4 months ago)

.


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:12 PM)

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23774585 - 10/26/16 08:07 PM (7 years, 4 months ago)

Soap and water in a squirt bottle? That's literally all I use..


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How to shroomery like a pro! (Seriously, everyone read this!)
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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Second Grow attemp ever, experienced shroomery users help please! [Re: Mad Season]
    #23774602 - 10/26/16 08:10 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:12 PM)

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: amidogen]
    #23774623 - 10/26/16 08:17 PM (7 years, 4 months ago)

I don't even wipe my shit with alcohol :shrug: I wash my hands in soap and water, ensure i have still af air, and NEVER have anything unsterilized over open media. I always pc my tools before doing sterile work too, which is actually more ocd than what others do xD.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Second Grow attemp ever, experienced shroomery users help please! [Re: Mad Season]
    #23774642 - 10/26/16 08:23 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:12 PM)

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: amidogen]
    #23774653 - 10/26/16 08:27 PM (7 years, 4 months ago)

Haha that was like me at least half a year ago. Probably nearing 10-12 months now since I ran out of alcohol. I haven't noticed any differences on my end :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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Offlinepatcorr_2001
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Registered: 10/21/16
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: amidogen]
    #23775999 - 10/27/16 09:15 AM (7 years, 4 months ago)

Okay I get your point about not using/needing iso. But just like you had said your first time you went hardcore sterile to take al the precautions you could think of, that's the only reason I wanted to use it. When you have your two first grows both get 100% contammed, it's depressing and your going to want to take every precaution necessary to make sure the next one doesn't turn out the same.

I'm so mad at myself man. I looked up this other guys first grow, he stared 38 jars the crazy bastard. I kid you not, 38 out of 38 reached 100% colonization without any contammed. I was so shocked, 38 out of 38 isn't that almost unheard of for an experienced grower using pftek/multi spore syringes? I know I shouldn't compare myself to another but I just hope these 7 jars so good. I'll be ecstatic if 4-5 make it.

Do you mind giving me step by step instructions mate for my inoculation procedure? I know it's a lot to ask for , but I want to be thorough and make sure NOT EVEN ONE little thing is done wrong on my part. It's my third try, and I feel I must succeed or this is it. Counting spores and supplies I'm sure I spent about 100$ CAD on my items, so to throw that all in the trash would be depressing.

Luckily my grow area is between 65-68 degrees then :smile: it's the only safe area I can use at the moment. The room is filled with a bunch of stuff stored in it, but as long as I disinfect the fuck out of the room with Lysol that's no biggie right ?

One more thing, is 50% iso strong enough to sanitize all of my equipment? I use hand sanitizer last time to sanitize some of my tools, you think that could of fucked my jars up lol?

PC

Edited by patcorr_2001 (10/27/16 09:17 AM)

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Offlinepatcorr_2001
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: amidogen]
    #23776206 - 10/27/16 10:24 AM (7 years, 4 months ago)

Went and got a spray bottle added soap and water , stopped at my local hospital and grabbed 5 face masks and walked out lol :smile: . Going to shower and change my clothes before I start cause it's been a good two days since I've done either one and don't want anything falling off of me onto my stuff after its sterile.

I have a bottle sterilizer made for baby bottles but it could maybe double as a sterilizer to sterilize my equipment . Going to use it then wipe everything down with isopropyl then get started . I should wipe all my inoculation points/ my micro pore tape down with iso before innoculaion too right ?
No need to give me step by steps , don't want to make you write all that when I'm pretty sure I got it down.

Start: Put on my gloves and wipe them down with ISO , put on mask.
1. Put soapy paper towels down in the bottom of the box
2. Spray soapy water all over the walls of the box.
3. Add everything already sterilized into the box. My jars, in one corner paper towels soaked with some iso(50% is what I got), my butane lighter , spore syringe .
4. Wipe down my inoculation points on my jar down with iso, heat up my spore syringe with the lighter , inoculate the four points of the jar after the syringe has cooled . Then wipe down the syringe with ISO paper towel, and repeat.

Does my methodology sound correct guys?? Or should I be doing something in there different ? Just want to be sure I'm doing this right before I start. Also I know I shouldn't put too much ISO on my paper towels , because the fumes can cause an explosion right ? Or is 50% too water based. Thanks .

PC

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Offlinepatcorr_2001
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776612 - 10/27/16 12:34 PM (7 years, 4 months ago)

2:15PM:Was waiting to see if you thought my technique would be good and sterile but I guess I'll get started as I don't want the jars to sit too long as I don't know if it can mess with the moisture level when sitting or not . Wish me luck mate


Edited by patcorr_2001 (10/27/16 01:39 PM)

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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776781 - 10/27/16 01:31 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:14 PM)

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Offlinethebug76
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776788 - 10/27/16 01:33 PM (7 years, 4 months ago)

Heres one tip. Don't let the syringe cool down first. The first drop or 2 of solution is going to cool it anyway, and as long as it's hot, it creates a thermal push that keeps unwanteds from landing on the needle.


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Bug 

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: thebug76]
    #23776792 - 10/27/16 01:34 PM (7 years, 4 months ago)

:whathesaid: it's called thermo push or some BS like that


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Offlinepatcorr_2001
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Registered: 10/21/16
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: amidogen]
    #23776807 - 10/27/16 01:41 PM (7 years, 4 months ago)

I read that taking your hands in and out the box or making too much movement makes a lot of air current move in and out of the box but your right the lighter may of made even more since a flame needs air to burn.. Hope they turn out okay.

3:28PM Update: threw away old PE jars, 6/7 blue molded and 7th one hasn't showed any myc growth in over 3-4 days , only had one little spot so chucked it with the others ..

My have each been inoculated with 1CC of TC . My SAB could of been a little bigger , but it was just perfect size to do my 7 jars. Had a paper towel soaked with 50% ISO in the SAB while I flame sterilized each inoculation , not gonna lie I was a little sketched out of the fumes possibly blowing the Lid off of my SAB and my gloves burning to my hands but luckily that didn't happen lol . Don't think 50% is very flammable but still ..

Thanks for all the info and help. I feel a lot more confident this time around after using an SAB , hopefully my attention to detail and carefulness I took this time pays off with some healthy myc/some nice fruits.
I will keep you updated on how the jars progress in the next 2-3 days. I read somewhere that TC is a pretty fast colonizer so I'm glad to hear that and at 70-73 F I think they should do well if healthy .

PC

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776816 - 10/27/16 01:43 PM (7 years, 4 months ago)

Also the fuckin SAB should have no air movement of your arms going in and out if you have large arm holes to reduce the piston effect. Ofc with large arm holes, your room you worm in must have no air flow going on for the box to be perfect :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Offlinepatcorr_2001
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: thebug76]
    #23776823 - 10/27/16 01:46 PM (7 years, 4 months ago)

Ah shit... See I let it cool for a good 30 seconds because I was scared that the micro pore tape would melt all over my syringe and would of caused my a headache, but by the way your making it sound looks like it wouldn't of melted it / I kind of fucked up. Now I'm worried lol,

1. Didnt flame sterilize outside the box
2. Didn't inject the spores whilst the needle was hot/let it cool down
3. Didn't mist the air over the box before adding the lid.

Looks like I could done a lot better lol, hopefully at least 4 of them make it and I'll be happy.

PC

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776838 - 10/27/16 01:51 PM (7 years, 4 months ago)

Bet it will be fine. Pf tek is so fool proof it isn't even funny. Most of the time contaminations are because of the syringe rather than techniques


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Offlinepatcorr_2001
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: Mad Season]
    #23776879 - 10/27/16 02:07 PM (7 years, 4 months ago)

I failed my first two pf teks dude lol I must really suck . First time using a stl air box this time. Both my syringes are over a year old can that make a difference ? Maybe my PE spore syringe was no good?

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InvisibleMad Season
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23776895 - 10/27/16 02:12 PM (7 years, 4 months ago)

PE has like the dirtiest spores ever. It doesn't drop spores so you gotta do some insane shit. Spore suppliers apparently use a centrifuge, which means a shit ton of contaminations on fruit bodies go in..

9/10 times people fail if they can't clean it on agar first. That I guarantee is the reason why. Your syringes. Actually I always say that if every jar goes down it has to be the syringe.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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Offlinepatcorr_2001
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Re: Second Grow attemp ever, experienced shroomery users help please! [Re: Mad Season]
    #23777150 - 10/27/16 03:20 PM (7 years, 4 months ago)

Alrighty , I'm sure I'm just panicking because of the two last epic failures . Ill keep this thread updated as soon as I notice any changes good or bad. Thanks.

PC

Edited by patcorr_2001 (10/27/16 04:39 PM)

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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Second Grow attemp ever, experienced shroomery users help please! [Re: patcorr_2001]
    #23777169 - 10/27/16 03:24 PM (7 years, 4 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:14 PM)

Extras: Filter Print Post Top
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