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Stopdroprole
Stranger

Registered: 04/13/13
Posts: 38
Last seen: 6 years, 6 months
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Growth in wbs without inoculation.
#23773495 - 10/26/16 02:32 PM (7 years, 3 months ago) |
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I have had some pressure cooked bird seed jars that never got inoculated lying around, extras. its been about 3 weeks left in a closet and one has what appears to be mycellium growing in the top of the seed, when i shook it most of the seed around it just stuck to it and coated the growth evenly and made a sphere. Could spores survive the pc? If not could any form of mold? Because this jar is closed entirely, only two holes filled with silica are on the lid, and it has never been opened. The lid is also new and the silica has never been pierced. I have only been pressure cooking for one hour and would remove the jars only 30 minutes after pressure went down. Could that be the reason?
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Growth in wbs without inoculation. [Re: Stopdroprole]
#23773556 - 10/26/16 02:55 PM (7 years, 3 months ago) |
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When you pressure cook grains, you're not killing everything that is living in them, but you're killing enough of the endospores off to create a window of opportunity where your mycelium can take over before the bacteria or molds can get a foothold. This window is about two weeks. If you don't inoculate in that time, there's a good chance contaminants will start to grow. Make grain jars when you know you'll use them immediately, or they'll start to grow on their own.
Also, 90 minutes is probably the minimum safe pressure cooking time for grains.
As a side note, I think you mean silicone, not silica. And why do you have two holes with silicone? There only should be one for jars you'll be injecting into. The other hole should be covered with a filter (SFD, Tyvek, Polyfill, etc.).
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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Th3Issu3
Stranger Danger


Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 1 month, 23 days
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Re: Growth in wbs without inoculation. [Re: Stopdroprole]
#23773559 - 10/26/16 02:56 PM (7 years, 3 months ago) |
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from my understanding even when you pressure cook for 90 mins at 15psi it kills everything for long enough to get the spores you inject into it to grow and take over, which prevents other ones from colonizing.
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
Posts: 745
Loc: ∅
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Re: Growth in wbs without inoculation. [Re: Th3Issu3]
#23773575 - 10/26/16 03:02 PM (7 years, 3 months ago) |
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It's true, I'm not sure how long it would have to be, but you've got to pressure cook for a pretty long time to legitimately kill everything. I guess I was more trying to say that 90 minutes is typically how long it takes to give you that two week window with the best chance of your myc taking over.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Growth in wbs without inoculation. [Re: Th3Issu3]
#23773578 - 10/26/16 03:02 PM (7 years, 3 months ago) |
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All molds are killed in a pc cycle...it's your filter.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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