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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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new to agar. need assistance please.
#23773369 - 10/26/16 01:42 PM (7 years, 3 months ago) |
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So I did searcxh old forums but couldn't find the clear answer I wanted. I recently got some agar from one of the sponsers. Fairly sure it was sporeworks. I got premixed malt extract agar. Bottle did NOT have amix ratio so I went back online to their website and it said 25g to 500ml water. Now I found where RR had advised 8.5g/500ml for spores and 10g/500ml for mycelium transfer. He said that was for agar agar. So I am assuming this is for PURE agar that is NOT premixed. Do you guys think me following what the site said, 25g/500ml water is to much? I just need advice as to how I should mix premixed malt extract agar for spore germination as well as mycelium transfer. I can't get on my computer to double check which sponser it was but I'm like 95% sure it was spore works. Bottle says rak media for brand if this helps anyone to be able to advise me. Thanks guys.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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You can't seperate the agar from the other ingredients so RRs notes are 100% meaningless to you. Just use what they say
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Captain67
Some geek dude



Registered: 06/24/16
Posts: 57
Loc: Canada
Last seen: 7 years, 1 month
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I recently just started getting into agar. I mixed malt extract and pure agar myself. 25g/500ml probably means it's 2:3 agar:malt ratio (10g agar and 15g malt), which is maybe a little more nutritious than what I mixed. Mine was more like 10g agar/12-15g malt (my scale was malfunctioning) per 500ml. My clones were growing very slowly and multispore didn't want to germinate so I started researching. The information I found all pointed to the nutrient content being too high. Also, I noticed my agar in particular seemed a bit too stiff, but I think that might have been my messed up scale causing the agar to be too concentrated..
The recommendation I found says to use more like 9 grams malt maximum instead of 12-15. I saw your thread literally as I was weighing out the mix for my new batch.
Quote:
hamloaf said:
Quote:
mindraped said: Here's my problem. I've got 8 agar jars that won't colonize. It's been 8 days since inoculation. Agar recipe was 30 mL MEA, 20 mL agar, and 1000 mL DI water. 5 were inoculated with fruit tissue and the others with a drop of spore solution. They all show signs of mycelial growth, but mycelium does not colonize the agar. What could be the issue here?
That's waaay to much malt and agar for just 1000ml of water. Mycelium will colonize slow as shit on subtrates with too much nutrition too. Generally, I use one third of that amount of ingredients in just half the amount of water (500ml). I tweek agar recipes depending on if I'm cloning or trying to germinate spores though. For 1000 ml of water only use twenty grams of agar and 18 grams of malt extract at the most.
Use their mix as-is, and if it's slow, you can try diluting the nutrient content with some pure agar. In the future, I'd recommend buying the separate parts so you can mix and tweak it yourself. Usually works out to be less expensive making your own anything IME Good luck I hope you enjoy learning agar as much as I have!
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: new to agar. need assistance please. [Re: bodhisatta]
#23773433 - 10/26/16 02:06 PM (7 years, 3 months ago) |
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That's kind of what I was thinking, that he was refering to pure agar. Would anyone advise me cutting it back slightly, to say 20g/500ml water for spore germination? I ask as I had read some ppl mix a "softer" agar for spore germination.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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No you're diluting the nutrition then. Besides using less agar is something you try if you're having difficulty with germination of spores
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numnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 5 months, 22 days
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Re: new to agar. need assistance please. [Re: bodhisatta]
#23773467 - 10/26/16 02:22 PM (7 years, 3 months ago) |
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Mine took awhile to germinate with spores as well. And some didnt germinate at all.
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Captain67
Some geek dude



Registered: 06/24/16
Posts: 57
Loc: Canada
Last seen: 7 years, 1 month
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IMO you're better off using your spores on PF cakes and cloning your favorite fruits. You can have 50 agar plates from spores and have no idea what the fruit is gonna look like, or potency. Unless youre ok with labeling, documenting and testing so many.. The only thing you can see on agar is which genes go more rhizo and which are more prone to fruit if you get in vitro pins, but ultimately that only goes so far.
-------------------- Synthetics betrayed me, and warped my mind. Nature mended it.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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Re: new to agar. need assistance please. [Re: bodhisatta]
#23773561 - 10/26/16 02:58 PM (7 years, 3 months ago) |
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I made up some agar, used one for mycelium and 3 for spores. been 5 days and spores still just sitting there in a clump. Also they came from a syringe
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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I'm doing spores on agar to try to isolate away from bacteria. I do have some cakes started. Some contaminated and some didn't. This is my very 1st agar try so I figured why not drop some spore solution and learn a few things and attempt to clean up some mycelium at the same time.I can't figure out how they can germinate on a cake in 5 days but not agar.
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Captain67
Some geek dude



Registered: 06/24/16
Posts: 57
Loc: Canada
Last seen: 7 years, 1 month
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I've had spores take 2 weeks to germinate on cake sub before. Sometimes it's just luck of the draw. Using a single spore syringe I had 1 jar half way colonized by the time another had germinated. Give it time. Sometimes it's 5 days and sometimes it's 3 weeks.
-------------------- Synthetics betrayed me, and warped my mind. Nature mended it.
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