Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24500326 - 07/22/17 07:26 AM (6 years, 6 months ago)

The CaCO3 and MG nutrients are classed as inorganic, so essentially the C-Gel agar is inorganic.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (07/22/17 07:39 AM)


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24506767 - 07/25/17 09:11 AM (6 years, 6 months ago)

The low carbon C-Gel agar works, and does not stall, I will be transferring using carbon rich pegs.
Use the C-Gel agar for extreme cleaning or validation of another transfer (2 step).

No contamination, discolouration or signs of any weirdness.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24506776 - 07/25/17 09:19 AM (6 years, 6 months ago)

The next T-Gel agar recipe will be based on eatyualive's, 50% tea extract 50% water method.


Recipe: 62g CaCO3 water, 62g Extract, 2.5g Agar, 0.2g 240B Gelatin, 0.1g MG.

Extract: 3.25g (1 bag) Black tea + 125g CaCO3 water.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: Ferather]
    #24510578 - 07/26/17 09:38 PM (6 years, 6 months ago)

Do you think your use of tea on agar will also have practical use in LC as well?? I'm interested in the tannin and polyphenol contents of tea as well as the acidic nature to deter growth of bacteria and mold in an LC jar, but perhaps for very narrow species of mushrooms.


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24510808 - 07/26/17 11:55 PM (6 years, 6 months ago)

Polysaccharide, polyphenol and tannic acid. With the first as food for your desired mycelium and the latter 2 as antibiotic to fight against contaminants. Don't you think this is how the T-gel works in a nutshell?? Also I think the C-gel might not work as good for acidic loving species e.g wood lovers.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24511453 - 07/27/17 10:13 AM (6 years, 6 months ago)

T-Gel works in two ways, by restricting the carbon source (no sugar, starch or cellulose), it requires laccase and protease enzymes.
The second is the antibacterial effects of the phenol carbon sources (tannin, catechins), an inhibitory carbon source.

By default a wood lover will decay inhibitory materials in wood, the tree's natural defences.
Not all woodloving mycelium like acidity, in fact BE with oysters is higher @ 7-8.

I don't use liquid cultures, and therefore cannot comment on it.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: Ferather]
    #24511479 - 07/27/17 10:33 AM (6 years, 6 months ago)

Have you seen this, Ferather?? https://www.shroomery.org/forums/showflat.php/Number/15736192/fpart/1/vc/1

Do you think they also have antibacterial properties like tea?? Do you think it will be better if amended with CaCO3?? I thought if that work so why tea wouldn't??

Also in my country there's a traditional wood tea drink made from Sappan Wood (relative to Brazil Wood) and they often sold in dried form so maybe I can do the same as link above with less wood to water ratio since they're dried?? What piqued my interest was the antimicrobial substance called Brazilin from these Sappan Wood (Biancaea sappan).
http://www.sciencedirect.com/science/article/pii/S1995764515000541

What do you think??


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24511603 - 07/27/17 11:50 AM (6 years, 6 months ago)

Wood extracts will work like tea extracts yes, just ignore that and call it "plant extract".
You want the soluble inhibitory (anti-microbial) materials, they are acidic.

The CaCO3 is used to achieve a desired end pH (not too acidic).
I've seen wood extract agar's, works of course.

Example plant products.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24511622 - 07/27/17 11:57 AM (6 years, 6 months ago)

Also to point out, the green machine is not only a mycelium, but also a woodlover.
Therefore other than higher pH, infection from spores is very likely.

So such thing as woodloving mediums without the green.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24511687 - 07/27/17 12:22 PM (6 years, 6 months ago)

Open air safe, non sterile, settings: 100% complex carbon source + pH 7-8.
No soluble or easy carbon sources + undesired pH (no activation).

You will still get "live" mold from spawn or transfers.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24511697 - 07/27/17 12:27 PM (6 years, 6 months ago)

You can also use my cellulose agar alternative, just control the end pH.
Paper pellets are 95-97% cellulose, no lignin, sugar etc.

25g pellets + (75g CaCO3 water + 1g MG).



--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: Ferather]
    #24512485 - 07/27/17 06:32 PM (6 years, 6 months ago)

Just like I suspected, CaCO3 amendment for plant extract is a must, eh??


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: Ferather]
    #24512492 - 07/27/17 06:34 PM (6 years, 6 months ago)

Btw, any alternative for MG???


Extras: Filter Print Post Top
InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24513399 - 07/28/17 05:57 AM (6 years, 6 months ago)

look for Nigari, it's magnesiumchloride.


--------------------
The best things in life
can be smelled on one's fingers.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: LizardWizard]
    #24513664 - 07/28/17 09:33 AM (6 years, 6 months ago)

No sodium or magnesium based pH adjustment, they have known toxicity levels, use calcium based alkali's only.
Any organic or non-organic "soluble nutrients", check the label, you want 1% nitrogen total added.

Gr0wer uses: Espoma organic plant-tone (5-3-3), Mycolorado uses: Age Old Grow (12-6-6).

I'm using Miracle-Gro (24-8-16), there are a few brands around.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: Ferather]
    #24513688 - 07/28/17 09:50 AM (6 years, 6 months ago)

Here are the results of the updated T-Gel recipe, twice as strong, twice as fast.
Sorry for the image quality, there is condensate on the lid currently.

Scrapings also grow out, but growth is best from the peg.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,692
Loc: the parking lot
Re: Antibacterial Tea Agar [Re: Ferather]
    #24513715 - 07/28/17 10:04 AM (6 years, 6 months ago)

Wait, hold up, you're trying to tell me Nigari has toxins in it or something? It's extracted out of sea salt, why would that be a problem? I don't see the point here, but maybe I'm not following... Please explain?


--------------------
The best things in life
can be smelled on one's fingers.


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: Ferather]
    #24513735 - 07/28/17 10:17 AM (6 years, 6 months ago)

Btw is it okay to use pour tek without sterilizing plates for T-gel??


Extras: Filter Print Post Top
OfflineDailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 2 days
Re: Antibacterial Tea Agar [Re: LizardWizard]
    #24513737 - 07/28/17 10:18 AM (6 years, 6 months ago)

Quote:

LizardWizard said:
Wait, hold up, you're trying to tell me Nigari has toxins in it or something? It's extracted out of sea salt, why would that be a problem? I don't see the point here, but maybe I'm not following... Please explain?



Sodium. Fungi hate that stuff.

Edit : Nevermind. I must be too tired, it was magnesium all along, I don't have much to say. Carry on.


Edited by DailyShroomer (07/28/17 10:21 AM)


Extras: Filter Print Post Top
OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24513964 - 07/28/17 12:47 PM (6 years, 6 months ago)

It's not a problem in small amounts, but both sodium and magnesium cause issues at certain amounts-ratios.
Calcium is safe because it doesn't cause toxicity, only growth reactions to pH level are an issue.

However CaCO3 will stop dissolving above pH 9-10, so it's not actually that bad for pH.
Additionally no one sodium or magnesium bathes straw, because it doesn't work.

Furthermore its suggested to use low magnesium lime for this reason.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* 'wood lovers agar' sakura 3,331 6 05/09/04 04:02 AM
by Mycena
* Do shiitakes prefer any particular agar recipe? Lothar 453 3 12/12/21 02:50 PM
by liamtheloser
* Reishi Tea Mycoangler 2,384 6 01/04/04 02:52 PM
by creekfarmer
* Reishi - ice tea Owl 2,874 5 08/22/05 08:54 PM
by sir_eel_green
* Liquid and agar culture of H. ulmatius? Baby_Hitler 1,541 4 05/19/04 08:41 AM
by charlieXxXxX
* Reishi Tea Recipe CptnGarden 7,065 2 05/16/04 10:37 AM
by CptnGarden
* Agar Anomalies - Black Morels and Oysters spores 2,553 1 05/30/05 05:28 PM
by BigGameHunter
* Agar recipe for cloning oysters "UPDATE PICS 16/12"
( 1 2 all )
Wheedhead! 9,297 28 12/16/03 04:53 PM
by G a n j a

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
9,987 topic views. 1 members, 23 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.007 seconds on 15 queries.