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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Yes, any live sample, eventually the tissue will decay, but you should get transferable growth.
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DailyShroomer
Stranger


Registered: 05/22/17
Posts: 106
Last seen: 8 months, 3 days
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Re: Antibacterial Tea Agar [Re: Ferather]
#24471838 - 07/10/17 06:26 AM (6 years, 6 months ago) |
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Can you use substitute for CaCO3 like with powdered eggshell for example?? I totally want to try this out.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Yes, egg shell is 95-97% CaCO3, other names: Chalk, Limestone, Oyster shell, Egg shell, Agricultural lime.
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DailyShroomer
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Registered: 05/22/17
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Re: Antibacterial Tea Agar [Re: Ferather]
#24471981 - 07/10/17 07:43 AM (6 years, 6 months ago) |
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Sweet!!
By the way, have you seen this? https://www.shroomery.org/forums/showflat.php/Number/17356799
Is the T-agar essentially the same as the link I'm referencing or is that just some sort of upgraded PDA?? It will be more efficient to use pour agar tek I think if you have smaller PC I think.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Thanks for the link, no the T-Gel recipe is not based of that. The recipe is based on carbon content and enzymes. However, I admit both the tea extract and CaCO3 are considered antibacterial, so yes there's a bonus.
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DailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
#24472044 - 07/10/17 08:22 AM (6 years, 6 months ago) |
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Can you use pour instead of no pour for T-gel like from my link?? I have a small electric PC, if can I'll buy loads of 45ml glass jars and start with them since it would be uber efficient to not PC the jars and just let the tea, CaCO3 and heat to do the work.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Yes, pour or no pour, entirely up to you. Handle as you would with any other agar.
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DailyShroomer
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Last seen: 8 months, 3 days
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Re: Antibacterial Tea Agar [Re: Ferather]
#24472083 - 07/10/17 08:50 AM (6 years, 6 months ago) |
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Sweet!
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DailyShroomer
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Oh, one more thing! What's the consensus on green tea?? Is it workable with the beef gelatin and the CaCO3??
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Yes, a few other community members have tried green tea, and got positive results.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24480435 - 07/13/17 02:34 PM (6 years, 6 months ago) |
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I added my transfer pegs yesterday, it has already begun transferring.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24486654 - 07/16/17 05:56 AM (6 years, 6 months ago) |
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The medium is more populated than I expected, as in there is not many gaps between the growth, a wide spread. There is also signs of genetic differences, several areas are much faster and denser than others.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24486657 - 07/16/17 05:56 AM (6 years, 6 months ago) |
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In conclusion, my T-Gel agar recipe works as intended, without sugar, starch and cellulose contamination dropped to none. I suggest assembling and working with it in a SAB, but open air is more than doable without a SAB-other. Only organisms capable of re-assembling materials into essential sugars will grow.
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DailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
#24488421 - 07/16/17 09:33 PM (6 years, 6 months ago) |
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This T-Gel works best for dirty wild samples, right? Oh, btw about green tea. How much green tea powder for 125 gr of CaCO3 water?? Green tea usually comes in dissolvable powder and I don't think 12 gr per 125 gr water is a realistic ratio, that'll be too much tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Any dirty sample, wild or not, the recipe I am using uses 1 tea bag, which is 3.25g. Given only the extract is used, I'd say that is up to 1g of extract, roughly.
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DailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
#24495218 - 07/19/17 10:34 PM (6 years, 6 months ago) |
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*noted
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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No problem, the recipe is cheap and easy to make, and works like other antibiotic agar's. I suggest pH strips, you can add lime to the tea extract to pH 7-8 (oysters).
Then add the mixture to the dry agar and 240B gelatin.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24496149 - 07/20/17 11:28 AM (6 years, 6 months ago) |
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I'm trying another recipe, using just CaCO3 as the primary carbon source. 125g > Boiling hot water + 10g CaCO3. 0.1g > MG soluble nutrients. 0.2g > 240B gelatin. 2.5g > Agar.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24496152 - 07/20/17 11:29 AM (6 years, 6 months ago) |
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I am transferring one of the pegs I produced using the T-Gel recipe.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#24500279 - 07/22/17 06:27 AM (6 years, 6 months ago) |
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The C-Gel agar is working, faster than T-Gel agar, and about the same speed as malt extract agar. Both the MG solubles as nutrients and CaCO3 as carbon, are working as I intended.
Growth is slightly thicker than T-Gel, however still radial as normal.
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