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OfflineFerather
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Re: Antibacterial Tea Agar [Re: ballsalsa]
    #23875063 - 11/28/16 12:13 PM (7 years, 2 months ago)

Quote:

ballsalsa said:
why lime? why not soda ash?



Sodium, while trace is essential, too much will inhibit growth, gross overdose will kill mycelium.


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OfflineChk
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Re: Antibacterial Tea Agar [Re: Ferather]
    #23877250 - 11/29/16 04:45 AM (7 years, 2 months ago)

Quote:

Ferather said:
Quote:

ballsalsa said:
why lime? why not soda ash?



Sodium, while trace is essential, too much will inhibit growth, gross overdose will kill mycelium.




Yes it without any doubt inhibit growth,
i tried doing PF-like cake with old bread (so it's around 15gr per Kg sodium chloride)
it worked but the colonization was damn slow, and the yield suffers.


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Chk]
    #23877304 - 11/29/16 05:42 AM (7 years, 2 months ago)

I tried steamed bread once, in a PC. Breadmakers add salt as a preserve.


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #23878505 - 11/29/16 02:41 PM (7 years, 2 months ago)

Turns out black tea is oxidized tea, not fermented, from source.


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OfflineChk
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Re: Antibacterial Tea Agar [Re: Ferather]
    #23878607 - 11/29/16 03:09 PM (7 years, 2 months ago)

Some (not all) black tea are fermented with Aspergillus Niger

https://en.wikipedia.org/wiki/Aspergillus_niger


"A. niger is the main agent in the fermentation of Pu-erh tea,[10] the Okinawan awamori spirit, and some varieties of shochu"


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OfflineDodgewelder87
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Re: Antibacterial Tea Agar [Re: Chk]
    #23878616 - 11/29/16 03:11 PM (7 years, 2 months ago)

help to identify please!


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Dodgewelder87]
    #23878622 - 11/29/16 03:12 PM (7 years, 2 months ago)

Commonly translated as dark, or black tea (this type of tea is different from what in the West is known as: black tea, which in China is called: red tea).

Pu-erh tea would be labelled "Pu-erh tea" and not black tea.


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Offlinekatbusa
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Re: Antibacterial Tea Agar [Re: katbusa]
    #23881982 - 11/30/16 03:04 PM (7 years, 1 month ago)

edited and updated


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: katbusa]
    #23882023 - 11/30/16 03:16 PM (7 years, 1 month ago)

Dam you beat me to it, so thats where you have been hiding. Very nice update!



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Offlinekatbusa
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Re: Antibacterial Tea Agar [Re: Ferather]
    #23882081 - 11/30/16 03:31 PM (7 years, 1 month ago)

Thanks for the kind words ferather!


I had this experiment totally finished but a coworker used my camera and deleted my photos. So I had to start all over again. I noticed this sitting in the back of a helicopter looking at previous powerline photos. I could of strangled the SOB.


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InvisibleMycolorado
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Re: Antibacterial Tea Agar [Re: katbusa]
    #23885730 - 12/01/16 08:24 PM (7 years, 1 month ago)

That blows!


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Mycolorado]
    #24454009 - 07/03/17 09:11 AM (6 years, 6 months ago)

I am trying another [experimental] recipe today, I've essentially eliminated sugars.
Here is the composition of tea, ready to drink, no additives (sugar, milk).

125g > CaCO3 water, boiling hot.
1 > Cheap black tea bag.
0.4g > 240B gelatin.
2g > Agar.

----

Stew the tea bag into the hot CaCO3 water using a jug, squeeze the bags on the sides for 5 minutes using a fork.
The idea is to extract as much from the tea bag as possible, increasing the carbon and nutrient value.

The CaCO3 water will help keep the pH in check as tea extracts are acidic by nature.
Add your tea extract and calcium water to your agar and gelatin powder.

----

Microwave the mixture for 40-80 seconds, if needed.


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OfflineLennybernadino
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Re: Antibacterial Tea Agar [Re: katbusa]
    #24458305 - 07/05/17 01:03 AM (6 years, 6 months ago)

:takingnotes:


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Lennybernadino]
    #24458745 - 07/05/17 08:59 AM (6 years, 6 months ago)

Well the "T-Gel" agar is working, my sample peg also has cellulose bacteria. It's very similar in speed as normal agar.
No signs of the cellulose bacteria transferring yet, the wood peg will also provide carbon and nutrients.

This recipe is intended to clean dirty samples, spore's should not germinate.

       


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24458775 - 07/05/17 09:19 AM (6 years, 6 months ago)

The peg will also receive nutrients from all through the agar, depending on the height of the peg.
This is due to evaporation, capillary action and mycelial uptake (distribution).


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24459278 - 07/05/17 01:41 PM (6 years, 6 months ago)

You got to love "pro mycelial" or "carbon controlled" medium's, that have a "live mycelium preferred" label.

List of carbon sources, remaining required nutrients from the tea and gelatin.
The nutrients come in a natural and organic water soluble format.


Calcium bicarbonate: [ Ca | C | H | O3 ] 2.
Polyphenols, varied: [ C6 | H5 | O1 ] x.
Proteins, varied: [ Cx | Hx | Ox | Nx ] x.

Theophylline: C7 | H8 | N4 | O2.
Theobromine: C7 | H8 | N4 | O2.
Caffeine: C8 | H10 | N4 | O2.
Theaflavin: C29 | H24 | O12.
Tannins: C76 | H52 | O46.
Catechin: C15 | H14 | O6.
Carotene: C40 | H56.


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24461360 - 07/06/17 08:07 AM (6 years, 6 months ago)

Strong, quick and fairly uniform growth, mycelium wins.

   


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24464543 - 07/07/17 09:19 AM (6 years, 6 months ago)

Sorry for today's image quality, I have condensate, the room hit 28°C, currently at 26°C.
Little regrowth on the peg, lots of mycelial activity on the T-Gel agar medium.

Tea turns black when oxidized, in this case due to laccase.
You should see a black ring around any radials.

   


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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24464933 - 07/07/17 11:54 AM (6 years, 6 months ago)

Here are better more clear images for today, no signs of any contamination.
Slightly slower than normal malt extract agar, however still quick.

   


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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24471633 - 07/10/17 02:30 AM (6 years, 6 months ago)

Can you just set and forget a foraged wild mushroom, say cordyceps into the T-gel without treating the mushroom body with iodine, iso, etc?

Edit : Sorry I misinterpreted your message on "sample" I thought it was mushroom fruitbody or something else but turns out you meant mycelium


Edited by DailyShroomer (07/10/17 02:34 AM)


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