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katbusa
TC Enthusiast


Registered: 02/19/13
Posts: 172
Last seen: 6 years, 5 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#23820331 - 11/10/16 04:44 PM (7 years, 2 months ago) |
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Awesome info ferather. So you think the T-Agar I sent you was lacking in nutrients? When you say you go growth on enriched tea. Are you talking about your enriched tea slurry tek?
If not can you shed some light on what nutrients you added to enrich with?
I would not have even thought that there was a nutrient issue for the fact that the tea was adding nutrients. I'm writing this down now and I'm going to try adding more MEA to the agar, maybe even start with the original amount of MEA and just add tea water to see what happens.
Just got home from working out of town! Got my indoor greenhouse set up. The plan is to get some results posted this weekend and dial in my winter greenhouse setup as well. Going to be a good weekend!
Edited by katbusa (11/10/16 04:47 PM)
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Antibacterial Tea Agar [Re: katbusa]
#23820921 - 11/10/16 07:46 PM (7 years, 2 months ago) |
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The recipe I used was based on yours, katbusa, but I reduced the tea by over half. It was around 2% tea and 1% ME. I was thinking along the same lines about MEA made with .5% or less tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Mycolorado]
#23822279 - 11/11/16 08:29 AM (7 years, 2 months ago) |
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All I can say is the samples looked naked and dormant, the other sample still does. 8 hours after rolling and placing the sample it began to return to life.
The ratio is not high enough, or the PC destroys nutrients.

Enriched with fertilizer and grain flour.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Ferather]
#23823434 - 11/11/16 03:52 PM (7 years, 2 months ago) |
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Found a university study on mycelium and yeast, here. Page two I read about digestion and absorption. Anyhow the answer is the solubility of the fertilizer. It can be instantly absorbed, and used.
The soluble fertilizer in this case counts as a nutritional accelerator. Too much becomes toxic, like adding 20% sodium.
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Antibacterial Tea Agar [Re: Ferather]
#23823898 - 11/11/16 07:01 PM (7 years, 2 months ago) |
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Not that anyone was eagerly awaiting this, but on the note of Ph: I did make a syringe of lime water and injected it into my tea LC. It's been about 4 days and the blue oyster in there is still doing nothing. It either really doesn't like the amount of tea in there or I didn't put enough honey, or both.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: flyontoast]
#23825019 - 11/12/16 06:41 AM (7 years, 2 months ago) |
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Hmm, I have little use for LC, however I can do some side tests at some point.
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Chk
Reverser


Registered: 09/06/13
Posts: 405
Loc: France
Last seen: 5 years, 3 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#23865478 - 11/25/16 08:46 AM (7 years, 2 months ago) |
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Just throwing that in there for someone interested: Oreganum vulgare essential oil is highly antiseptic.
it contains Thymol and Carvacrol https://en.wikipedia.org/wiki/Carvacrol
Quote:
Carvacrol inhibits the growth of several bacteria strains, e.g. Escherichia coli[6] and Bacillus cereus. Its low toxicity together with its pleasant taste and smell suggests its use as a food additive to prevent bacterial contamination.[7] In Pseudomonas aeruginosa it causes damages to the cell membrane of these bacteria and, unlike other terpenes, inhibits their proliferation.[8] The cause of the antimicrobial properties is believed to be disruption of the bacteria membrane.[9][10]
http://www.dailymail.co.uk/sciencetech/article-1088893/MRSA-wiped--OREGANO-say-British-scientists.html
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Chk]
#23865539 - 11/25/16 09:31 AM (7 years, 2 months ago) |
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Nice work, now I guess it needs to be tested on a fresh live mycelium sample. Have you found any data in your research in regards to mycelium?
Another thing to look at is spore germination.
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Chk
Reverser


Registered: 09/06/13
Posts: 405
Loc: France
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Re: Antibacterial Tea Agar [Re: Ferather]
#23865720 - 11/25/16 10:51 AM (7 years, 2 months ago) |
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Didn't test it,
it's while reading the OP that i remembered it has antibacterial effect i don't have any currently, could go buy some soon, there is an herbal shop close by.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Chk]
#23865735 - 11/25/16 10:57 AM (7 years, 2 months ago) |
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Thats your choice either way, if you do get some I am interested in results.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Ferather]
#23872356 - 11/27/16 03:03 PM (7 years, 2 months ago) |
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I've put your samples on T-Gel (Ph6), one Summer and one Tarragon, they both responded and are growing. Thank you so much again katbusa, Soon I should have both types growing on clean spawn.
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Antibacterial Tea Agar [Re: Ferather]
#23872439 - 11/27/16 03:27 PM (7 years, 2 months ago) |
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http://www.nature.com/articles/srep19710
Is some more detailed evidence supporting ferather. I am still a little skeptical of the antibiotic properties lasting through the PC but green tea would be the way to go.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: vatman]
#23872556 - 11/27/16 03:57 PM (7 years, 2 months ago) |
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Antibacterial Tea Agar [Re: Ferather]
#23872566 - 11/27/16 04:01 PM (7 years, 2 months ago) |
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Quote:
Ferather said: Active ingrediants: Catechins (175 °C), Tannins (200 °C), Theaflavins (240°C), Theanine (174 °C). Very pressure cookable 
most of those compounds stated are more anti axodaints. Some are anti microbes. The anti microbes are more on the green tea side. So why did you start off black tea over green tea?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: vatman]
#23872572 - 11/27/16 04:03 PM (7 years, 2 months ago) |
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My reason was cheapness and they are easy to obtain, but by all means go for green tea, much clearer.
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katbusa
TC Enthusiast


Registered: 02/19/13
Posts: 172
Last seen: 6 years, 5 months
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Re: Antibacterial Tea Agar [Re: vatman]
#23873253 - 11/27/16 08:01 PM (7 years, 2 months ago) |
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Quote:
vatman said:
Quote:
Ferather said: Active ingrediants: Catechins (175 °C), Tannins (200 °C), Theaflavins (240°C), Theanine (174 °C). Very pressure cookable 
most of those compounds stated are more anti axodaints. Some are anti microbes. The anti microbes are more on the green tea side. So why did you start off black tea over green tea?
I'm sure you know this but green tea and black tea are from the same plant. Black tea is just fermented. Green tea is the stuff you want to use but black can do if green is not available.
Tea does work but there have been some side effects. For example some of my cultures seem to grow pretty slow on the stuff. While on of my blue oyst cultures ripped right through it in a matter of a week. I'm not sure the correlation. I think it might be a nutrient issue. Why would tea change this I have no idea.
It is without a shadow of a doubt anti-bacterial. In fact I have been having a hard time getting some of my tea plates to contaminate. Even trich is really slow on the stuff.
I have quite a few experiments going on right now with tea. I just wish my work would allow me to be home more so I can wrap them up. Winter is supposed to be slow but apparently not this winter. Storm after storm is causing power companies to require my time away.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: katbusa]
#23874261 - 11/28/16 06:14 AM (7 years, 2 months ago) |
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Keep it up katbusa, plenty of holes I missed in research. My best result was at Ph 6 via lime, close to normal in speed. Bacteria was very aggressive @ Ph 4.5 - 5, Mycelium take acid damage over time, too in low Ph alone.
Blue and a few other types can tolerate Ph 4 - 9. King, Gold and others like Ph 6 - 8.
Bacterial activity at Ph 6 via lime is nil, void, zero.
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Pinpapa
Stranger than most


Registered: 09/01/12
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Re: Antibacterial Tea Agar [Re: Ferather]
#23874287 - 11/28/16 06:36 AM (7 years, 2 months ago) |
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Is that hydrated lime 4 adjusting.
-------------------- "This isn't the right thing to do , so let's go"
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Antibacterial Tea Agar [Re: Pinpapa]
#23874844 - 11/28/16 10:52 AM (7 years, 2 months ago) |
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Quote:
Pinpapa said: Is that hydrated lime 4 adjusting.
Dehydrated, powdered.
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I am making an infected LC from dirty WBS spawn, both bacterial and yeast. Using my own LC recipe, samples produced bubbles in 2 minutes.
When ready I will infect tea infused materials.
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ballsalsa
Universally Loathed and Reviled



Registered: 03/11/15
Posts: 20,876
Loc: Foreign Lands
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Re: Antibacterial Tea Agar [Re: Ferather]
#23875037 - 11/28/16 12:04 PM (7 years, 2 months ago) |
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why lime? why not soda ash?
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