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filyep
Dr.



Registered: 07/12/16
Posts: 131
Last seen: 3 years, 5 months
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Re: Antibacterial Tea Agar [Re: Ferather]
#23774087 - 10/26/16 05:37 PM (7 years, 3 months ago) |
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Great work guys. Yes if we could find a way of slowing down the unfavourable fungus that would be awesome.
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Quadman
Challenged


Registered: 04/23/16
Posts: 2,529
Loc: IL
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Re: Antibacterial Tea Agar [Re: filyep]
#23774138 - 10/26/16 05:51 PM (7 years, 3 months ago) |
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Benlate isn't organic, but perhaps to clean a culture an extremely low dose might work against mold.
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Edited by Quadman (10/27/16 08:51 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: katbusa]
#23775786 - 10/27/16 07:28 AM (7 years, 3 months ago) |
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So in terms of how antibacterial is tea, actually its a quadruple punch.
Catechins, Tannins, Theaflavins, Theanine.
Tea is perfect with grains.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Ferather]
#23775950 - 10/27/16 08:52 AM (7 years, 3 months ago) |
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Further investigation suggests lower temperatures are then next logical step. Even the slowest oyster mycelium can outgrow bacteria and mold.
Common molds are inhibited below 4°C, see here. I can grow oysters at 2°C, although slow.
Tea agar > Fridge (2°C) > Isolate.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Ferather]
#23775952 - 10/27/16 08:54 AM (7 years, 3 months ago) |
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Further actions:
> Reduce total water content, 50% or less. > Reduce O2 levels to minimum. > Reduce condensate.
Edited by Ferather (10/27/16 09:48 AM)
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katbusa
TC Enthusiast


Registered: 02/19/13
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Re: Antibacterial Tea Agar [Re: Ferather]
#23780515 - 10/28/16 04:12 PM (7 years, 3 months ago) |
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You are a wealth of information Ferather! I get immense joy reading about what you post. Thank you!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: katbusa]
#23782335 - 10/29/16 10:56 AM (7 years, 3 months ago) |
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Another thing you could do, if you have a dropper such as an eye dropper. Make a solution of apple vinegar and salt, and drop a bomb.
Selective destruction of trich effected areas.
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Antibacterial Tea Agar [Re: Ferather]
#23803525 - 11/05/16 02:20 PM (7 years, 2 months ago) |
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Has anybody tried doing tea as an LC? And do you think it could "clean" a contaminated LC? I did a tea bag with 1.5 cups of water and a teaspoon of honey (assuming tea has nutrients) and made 3 LC jars. I cloned an oyster into 1 of the jars to see if it would work (not seeing much action yet, maybe I didn't put enough honey, but it's dark as hell). Also have a jar of contam oyster LC and was wondering if there was a way to clean it without an agar transfer... I know the answer is 'probably not', but here's to hoping for a silver bullet.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: flyontoast]
#23803834 - 11/05/16 04:16 PM (7 years, 2 months ago) |
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Tea inhibits bacterial growth, it doesn't really count as cleaning or sterilizing. Hmmmm, try tea soaked card, then a few drops of LC in the middle.
The bacteria will likely be sugar or starch loving.
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Mycolorado
Hobbyist


Registered: 07/23/16
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Re: Antibacterial Tea Agar [Re: Ferather]
#23805737 - 11/06/16 08:46 AM (7 years, 2 months ago) |
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The tea agar I made is inhibiting pretty much all growth with the exception of blue oyster which is an animal. King oyster and a couple cubes are just sitting on it and a variety of spores won't germinate on it. I used Newman's Own and cut the tea content down. Too rich or other thoughts?
200ml H2O 4.25g black tea (2 bags) 2g ME 4g Agar

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Ferather
Mycological



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Re: Antibacterial Tea Agar [Re: Mycolorado]
#23805792 - 11/06/16 09:08 AM (7 years, 2 months ago) |
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My King oyster is also slow, with a black ring identical to yours. I'm using only tea, water and gelatin, no sugar/starch.
Currently mine is attacking a trich contam.
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Quadman
Challenged


Registered: 04/23/16
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Re: Antibacterial Tea Agar [Re: Ferather]
#23805912 - 11/06/16 09:49 AM (7 years, 2 months ago) |
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That blue is aggressive, wish all others behave that way. My tea soaked dowels all colonized well except the king was slow . There must be something with the tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Quadman]
#23805929 - 11/06/16 09:56 AM (7 years, 2 months ago) |
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Indeed very conclusive. And interesting, there's a particular chemical that is inhibiting it. Ultimately it's not a big deal, since we will be transferring, just time consuming.
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Mycolorado
Hobbyist


Registered: 07/23/16
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Re: Antibacterial Tea Agar [Re: Ferather]
#23805967 - 11/06/16 10:09 AM (7 years, 2 months ago) |
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My guess would be that the tannins are responsible. I'll make up some green tea plates to see how they do in comparison.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Antibacterial Tea Agar [Re: Mycolorado]
#23805978 - 11/06/16 10:13 AM (7 years, 2 months ago) |
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Awesome thanks mate, every test helps. I might try adding some soluble fertilizer.
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katbusa
TC Enthusiast


Registered: 02/19/13
Posts: 172
Last seen: 6 years, 5 months
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Re: Antibacterial Tea Agar [Re: flyontoast]
#23807743 - 11/06/16 06:43 PM (7 years, 2 months ago) |
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Quote:
flyontoast said: Has anybody tried doing tea as an LC? And do you think it could "clean" a contaminated LC? I did a tea bag with 1.5 cups of water and a teaspoon of honey (assuming tea has nutrients) and made 3 LC jars. I cloned an oyster into 1 of the jars to see if it would work (not seeing much action yet, maybe I didn't put enough honey, but it's dark as hell). Also have a jar of contam oyster LC and was wondering if there was a way to clean it without an agar transfer... I know the answer is 'probably not', but here's to hoping for a silver bullet.
Yes Im two weeks in on a tea water lc. I do not like how dark it is and will probably not use tea in LC again. I have several other tea based experiements running as well. I just havent had the time to do a full write up yet. Work has me tied down again. Working out of state leaves me limited time. Once Im back I plan on up dtating everything.
Also mycelium is slow on tea agar. One thing you should try is green tea. See if anything changes. Im also trying the green tea as well. Im starting to think that tea is going to be good for cleaning only. Or maybe the recipe is missing something.
Any thoughts?
Edited by katbusa (11/06/16 06:45 PM)
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katbusa
TC Enthusiast


Registered: 02/19/13
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Last seen: 6 years, 5 months
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Re: Antibacterial Tea Agar [Re: katbusa]
#23807837 - 11/06/16 07:24 PM (7 years, 2 months ago) |
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Guys I think I might be on to something. While sitting on the throne typing up my previous comments I had an epiphany.
This whole time the signs were there with the agar but I didn't pay attention to them. I noticed my agar was a bit soft in my plates compared to my non-tea plates. Both have the same amount of agar.
Low PH causes agar to not set as hard as it should. I have experience with this doing tissue culture for plants. If the PH is off the agar sets too watery. We use alot more agar than whats called for in tissure culture but the principle is the same.
I have heard that a low ph can stunt the growth. There also have been a few posts here and there on shroomery and others stating that lower PH caused slower runs / colonization.
So I did a quick test. Tea is pretty acidic. This could be why the mycelium is slow to run on tea agar.
My tap was at 7.0ph with tea im getting a ph down to 5 to 4 depending on the tests. I did two tests. PH test strips and vial dropper type.
If any of you havebthe ability to test please do so to verify what I just did. I was using green tea btw.

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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Antibacterial Tea Agar [Re: katbusa]
#23807930 - 11/06/16 08:02 PM (7 years, 2 months ago) |
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Low PH is an interesting variable. Would a pinch of lime adjust it, or would that further mess with the agar consistency? (I know nothing about agar, going to pour my first plates this week). I'm tempted to make a lime solution syringe and add it to my bluer oyster clone LC jar because the myc is really not doing anything. Might do that tomorrow if I've got the time.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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vatman
I'm Vatman


Registered: 04/17/14
Posts: 1,642
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Re: Antibacterial Tea Agar [Re: flyontoast]
#23807978 - 11/06/16 08:20 PM (7 years, 2 months ago) |
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Most mushrooms like an acidic environment and so does mold. Lime would slow your growth but it should still out compete mold. If I recall line is not recommend for agar though
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katbusa
TC Enthusiast


Registered: 02/19/13
Posts: 172
Last seen: 6 years, 5 months
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Re: Antibacterial Tea Agar [Re: vatman]
#23808013 - 11/06/16 08:36 PM (7 years, 2 months ago) |
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I know mushrooms like acidic substrates. I'm just wondering if the PH from tea is too acidic for them.
I'm not trying to rewrite the books on what is considered common knowledge but there seems to be some conflicting info on the inter-web-of-things. Some people say to keep the substrate neutral. Some say low PH is fine. Some say low PH im stunted growth and so on.
I did how ever find this paper for oyster.
http://jabonline.in/admin/php/uploads/58_pdf.pdf
It says they saw optimal growth at a PH of 7 with the highest yeild. It also said this :
" However, the biological efficiency, biological yield and economic yield were increased with the increase of pH levels up to 5.04 and then decreased"
Kind of conflicting.
I think I will try to adjust the PH to see what happens.
Edited by katbusa (11/06/16 08:50 PM)
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