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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23832130 - 11/14/16 12:45 PM (7 years, 2 months ago) |
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The bacteria should stall in 24-48 hours like before.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23834916 - 11/15/16 09:47 AM (7 years, 2 months ago) |
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Enriching the T-Gel is a no no, it increases bacterial activity x10, and counters all inhibitory chemicals. However, the yeast has stalled totally. The yeast infected Nameko has now only Nameko myc.

If you need more potency add more tea, use lime water to balance Ph if needed. I will make a second attempt for the Black poplar on normal T-Gel.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23839094 - 11/16/16 03:02 PM (7 years, 2 months ago) |
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I'm looking forward to seeing the black poplar
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Marty Mycfly]
#23840980 - 11/17/16 09:04 AM (7 years, 2 months ago) |
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I've decided to drop and replace my fertilizer for tea. More tea, more nutrients and antibacterial effects. Tea contains both soluble and non soluble nutrients and energy. So now on only tea fertilizer.
The enriched T-Gel Nameko test, has failed with yeast and a small amount of bacteria.
Adding anything other than tea should be considered a backwards step. Unless the materials used are antibacterial, and safe to use.

It is easy to spot contamination.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23840982 - 11/17/16 09:05 AM (7 years, 2 months ago) |
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So back to the original T-Gel recipe. I will do Black Poplar first.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23844783 - 11/18/16 10:08 AM (7 years, 2 months ago) |
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I made a batch of TGF-Tek today. After the microwave PC for 20 minutes, I took roughly 120g to use as spawn. It then was assembled and pressure cooked, the rest I have placed in the freezer, ready to cook.
The substrate is perfect, not sticky, well hydrated and crumbly after the microwave. I inoculated my sample with a King oyster peg from my first T-Gel test.
I'm doing 80% water content + 280g of water for cooking.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23848134 - 11/19/16 10:42 AM (7 years, 2 months ago) |
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Here is the King oyster peg onto TGF-Tek after 24 hours. Recovered and re-growing, all nice and clean.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23850626 - 11/20/16 07:06 AM (7 years, 2 months ago) |
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Conclusively the mycelium brings up nutrients on dead hydrated wood. Look no further than tea for a potent antibacterial fertilizer.
King oyster peg onto TGF-Tek after 48 hours.

Another peg onto plant food after 48 hours.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23857136 - 11/22/16 12:02 PM (7 years, 2 months ago) |
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I'm doing some further testing now on Ph, based on katbusa's findings. I am still waiting for my kit to arrive. However whilst I wait, I assembled some T-Gel using lime water that I made, just to test it out.
The gelatin set faster and harder, no obvious difference in colour or smell. I will use the same King oyster spawn used in my first T-Gel test.
Hopefully the lime does not neutralize any effects.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23859677 - 11/23/16 06:53 AM (7 years, 2 months ago) |
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Ok so after some research on the 4 active antibacterial ingredients in tea: Catechins, Tannins, Theaflavins, Theanine. I can only go as far as Ph 6 before they become unstable. Thankfully oysters like a Ph range of 6 - 8.
With a tea based substrate a casing of Ph 7 - 8 can be used when fully colonized.
If are you looking for quick energy then destabilize tea.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23859762 - 11/23/16 07:53 AM (7 years, 2 months ago) |
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Here is the pre Ph kit sample I just assembled.

I made another to Ph test.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23862860 - 11/24/16 07:59 AM (7 years, 2 months ago) |
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Best educated guess I have ever made, managed to get Ph 6.2 - 6.4.

No active bacteria on the new T-Gel, KO regrowing in 24 hours. Previous tests took 2-3 days for good regrowth.

No bacterial pooling or gelling.
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drake89
Mushroom Magnate



Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 4 years, 10 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23862912 - 11/24/16 08:19 AM (7 years, 2 months ago) |
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Mycelium almost always lowers pH to ~5 so when you raise it, it's just taking longer because growth is inhibited.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: drake89]
#23862939 - 11/24/16 08:30 AM (7 years, 2 months ago) |
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I knew about the mycelium producing acids, I did not equate the reduction though, so good point. On the lower Ph the bacteria stalled after 24 - 48 hours, so this will be interesting.
Will it stayed stalled, or grow a bit then stall again?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23863154 - 11/24/16 10:11 AM (7 years, 2 months ago) |
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Here is Ph 4 - 4.5 after 4 hours. Lots of bacterial activity.

Here is Ph 6.2 - 6.4 after 24 hours.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23863856 - 11/24/16 02:48 PM (7 years, 2 months ago) |
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This is the fastest and cleanest T-Gel yet, 30 hours:

No bacterial presence on the T-Gel.
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0yster
Lignicolous



Registered: 07/20/16
Posts: 19
Loc: World Wide
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23865530 - 11/25/16 09:26 AM (7 years, 2 months ago) |
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Edited by 0yster (10/23/18 07:57 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: 0yster]
#23868540 - 11/26/16 10:46 AM (7 years, 2 months ago) |
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Here is day 3, at 20 - 22°C, fully dark in a cupboard.

Image quality due to condensate.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23868668 - 11/26/16 11:24 AM (7 years, 2 months ago) |
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Why do you need 2 accounts?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Testing antibacterial starch free agars [Re: spacechildo]
#23868680 - 11/26/16 11:27 AM (7 years, 2 months ago) |
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Quote:
spacechildo said: Why do you need 2 accounts?
I dunno but check out that ridiculous self rating.
Edited by Inocuole (11/26/16 11:54 AM)
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