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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23809304 - 11/07/16 10:50 AM (7 years, 2 months ago) |
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Here is something I will attempt this evening or tomorrow. Using cocktail sticks (random link). All I do is cook them and pin the Gelatin. When ready transfer to T-Gel or Agar asap.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23809586 - 11/07/16 12:35 PM (7 years, 2 months ago) |
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The cocktail sticks took roughly 10-15 minutes to sink, not very long. Left to cool to room temperature. Simple Guide: Cut or snap the sticks, boil some water, stew the sticks in a container with lid.
I will be testing fertilized T-Gel to see the results of added nutrients. Also, I recycled the tea bag, and cooked it in grain water.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23809776 - 11/07/16 01:40 PM (7 years, 2 months ago) |
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So I pegged my T-Gel carefully in a SAB, It took seconds for the cling film to attract dust after removing. I used sterilized metal tweezers to remove the pegs and place them into my T-Gel sample.
In a few days I can attempt another transfer to T-Gel.

These are made from birch wood.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23811908 - 11/08/16 07:46 AM (7 years, 2 months ago) |
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18 hours later, it has started to grow on the birch wood pegs. When ready I will test a transfer to enriched T-Gel.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23815472 - 11/09/16 08:06 AM (7 years, 2 months ago) |
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I will be able to transfer this evening, or tomorrow.

The trich suffers greatly in high CO2.
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23816367 - 11/09/16 02:20 PM (7 years, 2 months ago) |
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Not fully catching the purpose to the sticks. You are letting the sticks get colonized and then are going to transfer the stick to a new dish rather than cut-out a wedge?
Separate question: how long can you leave myc on gelatin for? I was reading-up on pectin-"agar" and most people were saying that the myc digests the gelatin and the plate turns into a puddle. Is that not your experience, or is it true but you just don't leave the myc on one plate long enough for that to be a problem?
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: flyontoast]
#23816511 - 11/09/16 03:02 PM (7 years, 2 months ago) |
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The sticks are being used to transfer, correct. There are three types of Gelatin: Acid, alkaline or water extracted. So far the strong Gelatin (acid) has stayed whole, no obvious mycelial liquefaction, still firm.
One transferred, The sample longer is no longer required. The purpose is to clean, isolate and transfer.
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flyontoast
Farming food; farming time


Registered: 08/20/16
Posts: 258
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23817416 - 11/09/16 07:34 PM (7 years, 2 months ago) |
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 Did you come-up with this stick idea or was it already a tek? It's a brilliants device for making deep-dish and pre-poured agar plates easily workable,and all you need is tweezers! These colonized sticks would probably makes great slants too. I dunno, but when you combine pre-poured "agar" dishes, without the need for a scalpel, and less paranoia about bacteria, it really makes working with and storing plates a super low bar of entry for noobs. Not to mention yanking a handful of sticks out a plate to dump into grain has got to take way less time than making that many wedges. I'm PCing a pre-poured agar now and am going to jam all sorts of toothpicks and chopsticks and whatever other sticks I have into it.
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My trade list Looking for strong terrestrial fruiters for an outdoor beds experiment: Agaricus Bitorquis, Agaricus Augustus, Agaricus blazei/subrufescens, Stropharia Rugoso-annulata, Clitocybe Nuda (blewits), and any species or other genus that you think work outdoors. Also, any commercially viable Pleurotus, cold or hot strains. Thanks for the Q&A, trades, and all the posters & teachers that have come before us
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: flyontoast]
#23818684 - 11/10/16 08:14 AM (7 years, 2 months ago) |
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Indeed flyontoast, the easiest transfer I have ever done, by far. It took about 45 seconds.

If this was a super rare wild clone, I now have a clean strong sample. And yes this tek was my idea. It is free for all to use.
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0yster
Lignicolous



Registered: 07/20/16
Posts: 19
Loc: World Wide
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23819752 - 11/10/16 12:57 PM (7 years, 2 months ago) |
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Edited by 0yster (10/23/18 07:57 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: 0yster]
#23819874 - 11/10/16 01:43 PM (7 years, 2 months ago) |
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After this test I will be transferring Black Poplar spawn to T-Gel. Depending on test results, either normal or enriched.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23820020 - 11/10/16 02:46 PM (7 years, 2 months ago) |
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Quote:
Ferather said: Indeed flyontoast, the easiest transfer I have ever done, by far. It took about 45 seconds.

If this was a super rare wild clone, I now have a clean strong sample. And yes this tek was my idea. It is free for all to use.
That's a thing of beauty, Billy!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Mycolorado]
#23820045 - 11/10/16 02:56 PM (7 years, 2 months ago) |
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If I did not have trich, I could have continued with open air assembly throughout. However, the trich has not passed on the transfer, and I am successful.
My intention now is to test a variety of cultivated mycelium. When complete I will post the T-Gel tek in full.
Any testing and input is welcome.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23822283 - 11/11/16 08:31 AM (7 years, 2 months ago) |
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Here is 24 hours after the birch peg transfer.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23822813 - 11/11/16 12:04 PM (7 years, 2 months ago) |
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You are a pirate Ferather! Good work!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Marty Mycfly]
#23823565 - 11/11/16 04:55 PM (7 years, 2 months ago) |
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Well the only thing to do now, other than side experiments, is test various mycelium and clean them. If I can turn dirty spawn clean, I can clean anything that likes T-Gel, or adjusted T-Gel.
That means I can clone wild mushrooms, clean dirty vendor spawn, and so on. Mycolorado tested several types of spores, most failed to germinate.
However that was T-Agar and not T-Gel, inconclusive.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23825472 - 11/12/16 10:22 AM (7 years, 2 months ago) |
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Here is 50% less tea but enriched with soluble nutrients, 48 hours. Next I will try the normal amount of tea but enriched.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23831796 - 11/14/16 10:17 AM (7 years, 2 months ago) |
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So the King oyster was getting most of its energy and nutrition from the tea solubles. The 50% less tea + nutrition is only half as strong as full tea power.
I have setup full power enriched for testing.

Peptides provide 2% energy overall.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23831797 - 11/14/16 10:17 AM (7 years, 2 months ago) |
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Here we have full power enriched, but different mycelium. Black poplar and Nameko. Left, Black poplar, bacteria infected. Right, Nameko, yeast infected.
Both samples may be infected with mold.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23832127 - 11/14/16 12:43 PM (7 years, 2 months ago) |
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Results in 2 hours, the bacterial Black poplar is the same as the bacterial King oyster. Bacterial liquefying and some sinking. Some myc debris growing on the right.
The yeast infected Nameko has no liquefying, no yeast activity.
Here is the bacterial Black poplar sample.

Both samples have fresh growth.
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