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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Testing antibacterial starch free T-Gel
#23770605 - 10/25/16 04:28 PM (7 years, 3 months ago) |
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Decided to join katbusa at testing ways to make antibacterial agar mixtures. I will be using sugar free jelly, so I can make a test run tomorrow.
So far katbusa has been successful with tea slurry agar. I intended to test additional additives, and ratios.
And yes I have a powerful torch.
Edited by Ferather (11/10/16 04:02 PM)
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katbusa
TC Enthusiast


Registered: 02/19/13
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23771157 - 10/25/16 07:23 PM (7 years, 3 months ago) |
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I will be writing up my tea agar methodology for you soon. Ive been super busy the last few days. Now that I think about it I might do it now since Im being lazy and sitting on my couch.
Edited by katbusa (10/25/16 07:23 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: katbusa]
#23772285 - 10/26/16 04:06 AM (7 years, 3 months ago) |
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Just read it, great writeup so far. And I'm joining in the fun. Everyone feel free to join in, post here if you like.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23790569 - 11/01/16 07:38 AM (7 years, 2 months ago) |
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Triple test, I am using extra strong gelatin, and tea water. No starch. Open air assembly.
Test A, will I get an infection from the air on this composition. Test B, will the gelatine turn to liquid, most probably. Test C, will the mycelium expand and grow.

Set like silicone, hard to penetrate.
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Mattisfat
Learning


Registered: 01/20/16
Posts: 570
Loc: Newcastle, England
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23790603 - 11/01/16 08:01 AM (7 years, 2 months ago) |
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what you need to do is get a bacteria culture on agar - swab that onto a standard agar recipe and then swap it onto the tea recipe so you can see the difference in growth side by side.
maybe drop some mycelium in each container aswell to see how that does next to the bacteria.
Too many variables in your current way to get a clear view of the difference.
Currently im trying 10g black tea 9g malt extract 9g agar 500 ml water as im struggling with bacteria contams atm hopefully tea might help.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Mattisfat]
#23790925 - 11/01/16 10:33 AM (7 years, 2 months ago) |
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Yea this one is semi un-serious due to the high chance the gelatin will turn to liquid. Decided since that is going to do that, may as well test a few other things.
You are right though, just using up this gelatin.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23791750 - 11/01/16 03:33 PM (7 years, 2 months ago) |
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4 hours, the gelatin seems to liquefy (digestion) then set again.

Mycelial debris growing, too fast for spores. Will most likely end with a liquid.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23793835 - 11/02/16 07:13 AM (7 years, 2 months ago) |
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Turns out the liquefying was bacteria, the good news is, it has pretty much stalled. The mycelium from the grain is growing out roughly at normal speed.
No obvious liquefying by any of the mycelium either.
Seems to be working, Images soon.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23793848 - 11/02/16 07:25 AM (7 years, 2 months ago) |
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Side note:
No infection from open air assembly, most likely due to 0% starch and sugars. This particular recipe will likely only work with live transfers.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23800794 - 11/04/16 02:21 PM (7 years, 2 months ago) |
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Well, it works as intended, bacteria stalled after 24 hours, the mycelium won by day 4. Not a single infection from open air assembly, possibly got lucky.
The strong gelatin is working 100% like agar.

No mycelial liquefying.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23800828 - 11/04/16 02:34 PM (7 years, 2 months ago) |
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P.S. this was microwaved for 88 seconds then left to set, no pressure cooker.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23800951 - 11/04/16 03:26 PM (7 years, 2 months ago) |
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Wow, that's pretty interesting! Only 88 seconds in the microwave?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Marty Mycfly]
#23801181 - 11/04/16 04:48 PM (7 years, 2 months ago) |
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Well not 100% true, for the whole recipe, which is as follows:
> 20g extra strong gelatin, 12g tea. > 500g freshly boiled water.
Stew the tea for 5 minutes using a spoon, squeezing the bags. Microwave for 2 minutes, and repeat again one more time.
Remove the bags and then microwave for 2 minutes. Add the gelatin and microwave for 88 seconds.
Use as normal and leave to set, covered.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23805789 - 11/06/16 09:08 AM (7 years, 2 months ago) |
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Today, Trich, under attack from King oyster.

The trich is weak, and cut off.
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Quadman
Challenged


Registered: 04/23/16
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23805916 - 11/06/16 09:50 AM (7 years, 2 months ago) |
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Weak and cut off, but unfortunately still there.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
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Re: Testing antibacterial starch free agars [Re: Quadman]
#23805939 - 11/06/16 09:59 AM (7 years, 2 months ago) |
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If the King destroys or outgrows it, it may inhibit it enough to prevent further growth. That would allow me to continue growing and isolate a clean sample.
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Quadman
Challenged


Registered: 04/23/16
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23806034 - 11/06/16 10:32 AM (7 years, 2 months ago) |
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If you want clean I'd be transferring from the opposite side now. That mean green will be all over with more time. You can let it grow out after the transfer for experimental purposes.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Quadman]
#23806052 - 11/06/16 10:40 AM (7 years, 2 months ago) |
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I have stupid amounts of spawn, this is just a test. I isolated a sample and left it out for 8 days. Got both bacteria and mold, the bacteria may well have already been there though.
Good enough for testing, not going to buy bacteria or make mold.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23808892 - 11/07/16 08:35 AM (7 years, 2 months ago) |
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Well, the King is eating the trich for dinner.

The trich has also receded.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Testing antibacterial starch free agars [Re: Ferather]
#23808945 - 11/07/16 08:58 AM (7 years, 2 months ago) |
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Name of recipe: T-Gel Tek, read my copyrights.
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