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Edmunter
Mr



Registered: 05/01/13
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Mexicana grow
#23770115 - 10/25/16 01:51 PM (7 years, 3 months ago) |
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Im using an PADY of 10;10;7;1 which works fine for cubensis. However it is a bit slow more Mexicana and im not sure why.
The best growth im getting is from the initial spore swipe. Is it advisable to use a wedge from that to inoculate a jar?
I have loads of wedges growing VERY slowly but im bored and need more jobs...........
Edited by Edmunter (12/12/16 02:37 AM)
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c10h12n2o
serial dilutor



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Re: Mexicana grow [Re: Edmunter]
#23770133 - 10/25/16 01:56 PM (7 years, 3 months ago) |
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im confused by your question, mind clarifying?
what do you mean when you say you have wedges growing? typically, you streak spores to isolate clean growth by transferring it, then keep doing transfers if you want an isolate or more organized culture
this is the way i do it, saves tons of time:

this gives you lots of colonies to choose from, with various levels of strain diversity
then once you have a clean, organized culture, you can do a tiger drop (tic tac toe) to grain for a master or whatever. or you could make a liquid inoculant. but no, you definitely dont want to use the plate which you originally streaked
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Edmunter
Mr



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Yep do all that already but never used an original plate.
Why not use it?
I love ur little diagram but I need a loop as my sterilised cotton bud arnt long enough.
I usually swipe, transfer, grow, clone, grow but i have no equipment thats not being used. Its like a factory here and the missus is going nuts. Have a jar of grains and thought why not........ and u can answer that.
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bodhisatta 
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Re: Mexicana grow [Re: Edmunter] 1
#23770231 - 10/25/16 02:28 PM (7 years, 3 months ago) |
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The plate you put your spore on the initial plate may have un-germinated contamination that you may inadvertently put into a grain jar. It's best to use a subsequent plate to avoid that
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c10h12n2o
serial dilutor



Registered: 01/21/15
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i still dont understand what you mean by "wedges growing" though? im confused by the context you use the word "wedge" in
cotton for streaking agar? thats crazy, definitely start using something you can flame sterilize. standard lab inoculation loops are the bomb, and dirt cheap, but it is dead simple to make your own as well: all it is is wire made into a loop and twisted around itself, with an optional handle.
it isnt possible to follow the diagram's technique with a swab, since the utensil has to be flame sterilized at 4 different points, and using 4 different swabs is begging for problems
lol many of us can certainly sympathize with trying not to clutter up the lady of the house's kitchen and other areas, ive tried to build some stuff to keep everything contained and out of sight and way as much as possible and generally plan better, and that has helped tremendously
i wouldnt inoculate with a plate that was less than 100% colonized if i could help it (not necessary but speeds things up) and definitely a few transfers from spores. it usually takes a few transfers to get an organized culture and be sure its free from bacterial hobos
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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filthyknees
no coincidence


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Re: Mexicana grow [Re: Edmunter]
#23770573 - 10/25/16 04:21 PM (7 years, 3 months ago) |
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If your goal is sclerotia formation try 10% coffee agar
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Edmunter
Mr



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Quote:
c10h12n2o said:

Let me try to make it simple for you -----
i still dont understand what you mean by "wedges growing" though? im confused by the context you use the word "wedge" in
--------------------------------------------- A wedge is a small piece I cut out from a growing culture and either put in a jar, blend up in a LI or grow out into another culture. So when I say I have wedges growing it means I am growing cultures from wedges....not clones, not spores.....ect
cotton for streaking agar? thats crazy, definitely start using something you can flame sterilize. standard lab inoculation loops are the bomb, and dirt cheap, but it is dead simple to make your own as well: all it is is wire made into a loop and twisted around itself, with an optional handle.
---------------------------------------------- Might seem crazy but if u put cotton buds in silver foil when u sterilize jars they are good to go. Never have a problem.
it isnt possible to follow the diagram's technique with a swab, since the utensil has to be flame sterilized at 4 different points, and using 4 different swabs is begging for problems
lol many of us can certainly sympathize with trying not to clutter up the lady of the house's kitchen and other areas, ive tried to build some stuff to keep everything contained and out of sight and way as much as possible and generally plan better, and that has helped tremendously
i wouldnt inoculate with a plate that was less than 100% colonized if i could help it
------------------------------------- I have 10 different spawn jars growing al sorts of different things, im only curious because ive never thought about doing this before and the wedges/transfers/new cultures are growing slowly. I have over 40 dishes in use atm
(not necessary but speeds things up) and definitely a few transfers from spores. it usually takes a few transfers to get an organized culture and be sure its free from bacterial hobos
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Edmunter
Mr



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Quote:
filthyknees said: If your goal is sclerotia formation try 10% coffee agar
Will do
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: Mexicana grow [Re: Edmunter]
#23770723 - 10/25/16 05:05 PM (7 years, 3 months ago) |
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what horrible formatting, almost as hard to interpret as your syntax and misuse of jargon 
also take a look at the forum rules. try to use the "edit" button rather than double posting
lol your attempt to be a smartass is quite flaccid, but thanks for "making it simple for me." how bout try making sense in the future, or at least not acting like a dickhole when someone asks you for clarification while answering your question
what you have growing are cultures on plates, not "wedges." you could take a wedge from a culture, or use a wedge from a culture to inoculate anything from another plate to grainspawn (and a thousand other things), but the fact that it is shaped like a wedge doesnt tell us anything about whether it is MS, monoculture, clone, MEA, or anything else
This sentence makes no sense at all, and suggests that you have a poor understanding of what any of these words mean (no offense, you just need to do some reading):
Quote:
So when I say I have wedges growing it means I am growing cultures from wedges....not clones, not spores.....ect
And as far as the cotton, you are entirely missing my point. of course it could work if you sterilize it, but for that matter you could use your finger and it might work, that doesnt make it a good idea. might as well be using a sponge from your sink
the point of streaking a plate with a loop is to have very controlled amounts of tissue or spore transfer and spread out inoculant to form multiple distinguishable colonies. using a swab could smear something around, but flame sterilizing is easier and more suited to the task of streaking plates than pressure cooking q-tips
you are completely misunderstanding lots of vocabulary words and techniques. sterile swabs are usually used to swab the gills of fruits (especially fruits that are tough to get to drop spores), and wrapped in foil to be traded like spore prints. There are several ways to use these spore swabs, but the sloppiest would be to touch agar with it (which would not be good for long term storage of the swab after either). The best way is to simply scrape the spore swab with a flame sterilized inoculation loop and then streak a plate, and transfer clean colonies to new plates (avoiding any visible contamination). Alternatively, you could use a flame sterilized scalpel blade in a pinch: it would be much harder to finesse but would be infinitely better than whatever the hell you are doing with a swab on agar
@bodhi: ......speaking of swabs..... lol im still down to amazon prime you some swabs and foil and whatever else you need, ya greedy bastard
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Edmunter
Mr



Registered: 05/01/13
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I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
Edited by Edmunter (10/26/16 09:56 AM)
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cronicr



Registered: 08/07/11
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Re: Mexicana grow [Re: Edmunter]
#23772338 - 10/26/16 05:05 AM (7 years, 3 months ago) |
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Sup munter?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



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Re: Mexicana grow [Re: cronicr]
#23772465 - 10/26/16 07:16 AM (7 years, 3 months ago) |
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Hey Cron, been a while. Hows the music going bro?
Seems im getting my wording all twisted so nobody understands.
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cronicr



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Re: Mexicana grow [Re: Edmunter]
#23773238 - 10/26/16 01:01 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: Im using an PADY of 10;10;7;1 which works fine for cubensis. However it is a bit slow more Mexicana and im not sure why. If they are getting sluggish on agar switch the media up...I find they get lazy fast. The best growth im getting is from the initial spore swipe. Is it advisable to use that as a wedge for a jar?
I have loads of wedges growing VERY slowly but im bored and need more jobs...........
try switch your media up it might simply be getting lazy
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: Mexicana grow [Re: Edmunter]
#23773253 - 10/26/16 01:06 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you
i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument
if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."
Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" 
if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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cronicr



Registered: 08/07/11
Posts: 61,436
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Last seen: 2 years, 9 days
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You might think your long winded garbage posts make you a winner here but you are far from right knock the crap off.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mexicana grow [Re: cronicr]
#23773382 - 10/26/16 01:48 PM (7 years, 3 months ago) |
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is there a game going on? i was not aware some people type/think faster than others
some people will always find something to get offended by, but others actually come here to read and learn, and appreciate concise information. still hard for me to understand how people get offended by it though
not sure what "garbage posts" you are referring to, i think i answered his question pretty effectively, and clarified the vocab for anyone reading
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (10/26/16 01:58 PM)
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cronicr



Registered: 08/07/11
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The long winded five paragraph off topic crap...
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mexicana grow [Re: cronicr]
#23773455 - 10/26/16 02:15 PM (7 years, 3 months ago) |
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lol my posts explaining the problem with streaking with a q-tip and offering a better suggestion? or the one where i reminded about not double posting? the one where i explained why OPs phrasing was confusing, and clarified for the sake of anyone reading who is actually trying to help or learn something? or the one where i reiterated that cultivation advice is not personal and shouldn't offend anyone?
i dare say ALL of my posts in this thread are more on topic and less crap than:
Quote:
cronicr said: Sup munter?

but what do i know, apparently you think its worth the ban warning you just sent me. I was not aware that telling someone not to get offended by cultivation advice was against the rules, but i guess its your playground, whatever makes you feel better
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (10/26/16 02:27 PM)
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Edmunter
Mr



Registered: 05/01/13
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Is this all because I said wedges instead of cultures that I know nothing?
You are the first person to be a proper insulting dick here to me.
Im a humble guy trying to get some answers from a community of pretty cool people.
You need to calm down and stop trying to outsmart people.
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cronicr



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Re: Mexicana grow [Re: Edmunter] 1
#23773513 - 10/26/16 02:37 PM (7 years, 3 months ago) |
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Well now he is taking a break back on topic people..munter try switch your media up it helps with them.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



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Re: Mexicana grow [Re: cronicr]
#23773524 - 10/26/16 02:40 PM (7 years, 3 months ago) |
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What would you suggest for Psy Mex?
My 'WEDGES' are a bit fluffy on some and I thought it might need something else.
WEDGES = Chunks of culture I cut out in the shape of a wedge.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Mexicana grow [Re: cronicr]
#23773544 - 10/26/16 02:51 PM (7 years, 3 months ago) |
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Quote:
cronicr said: Well now he is taking a break back on topic people..munter try switch your media up it helps with them.
What's this supposed to mean? Did C10 really just get banned for this shit? If so, your "Moderator" status and or qualifications are highly suspect.
The OP was using jargon incorrectly or at the very least using terms in a way that aren't commonly use around here. Was C10 a bit abrasive? Maybe. Was he out of line and deserving to be banned? No Fucking Way.
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Edmunter
Mr



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Can somebody tell me what the fuk I said that was so mind bendingly confusing?
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cronicr



Registered: 08/07/11
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Re: Mexicana grow [Re: Edmunter]
#23773562 - 10/26/16 02:58 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: What would you suggest for Psy Mex?
My 'WEDGES' are a bit fluffy on some and I thought it might need something else.
WEDGES = Chunks of culture I cut out in the shape of a wedge.
Any type of a switch up will do.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



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Re: Mexicana grow [Re: cronicr]
#23773606 - 10/26/16 03:10 PM (7 years, 3 months ago) |
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Is rye water/agar any good for these?
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tombosley8
Full on... Bossley Baggins



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what munter said sound fine to me.
maybe he could have said a culture grown from a wedge cut from another plate of cultured agar but that's a lot more than needed. we all know what growing from wedges means right?
and I think we knew what he meant when he said he is growing out wedges.
Meaning he didn't noc the plate directly with spores, clones, etc. Meaning he did noc the plate with a wedge from another agar plate.
good enough for me
Either c10 was confused or he just wanted to argue about something that was maybe not perfectly grammatically correct
Sure I understand jargon needs to be held to the definition but I truly think munt was using it in close enough to the right context.
Now that I had to rant and go off topic to explain I will say I usually use swabs to noc agar with spores and haven't had much problems after a few transfers.
Maybe loops can help with spreading the spores away from contam and getting single colonies but I've never tried and didn't think that was so necessary... I have tried with my scalpel a couple times and just seemed to get less growth due to less spores but the colonies were still all mixed together.
Either way you can still get a good organized clean culture with a few transfers.
so back to the point. Cron is right on switching up the nutes for your agar. It helps a lot.
Dog food agar is super cheap and easy just as easy as MEA just isn't as clear but that shouldn't matter anyway.
Just grind up dog food in a coffee grinder or blender and use in place of malt extract.
I use slightly less nutes in my agar when making transfers for faster more rhizo growth. but when transfering to grain, lc, etc you want the regular nute level for strongest and fastest leap off. And spore germination should be the normal recipe too.
so recipe normally is 10g dog food 10g agar 500ml water
I use 8g dog food 10g agar 500ml water
start a few cultures of the same print so you can choose the strongest cleanest from them and discard the questionable ones.
Edited by tombosley8 (10/26/16 03:21 PM)
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Mycolorado
Hobbyist


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Re: Mexicana grow [Re: cronicr]
#23773644 - 10/26/16 03:20 PM (7 years, 3 months ago) |
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So this is how it works around here? You didn't ask anyone to stay on topic anywhere. C10 answered his question and gave him some additional and helpful info. And when I ask what's up, you send me this:

Get off your power trip.
Back on topic so I don't get banned too...a lower nute content might prove beneficial in producing more aggressive mycelial growth.
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 11 hours
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Quote:
tombosley8 said: what munter said sound fine to me.
maybe he could have said a culture grown from a wedge cut from another plate of cultured agar but that's a lot more than needed. we all know what growing from wedges means right?
and I think we knew what he meant when he said he is growing out wedges.
Meaning he didn't noc the plate directly with spores, clones, etc. Meaning he did noc the plate with a wedge from another agar plate.
good enough for me
Either c10 was confused or he just wanted to argue about something that was maybe not perfectly grammatically correct
Sure I understand jargon needs to be held to the definition but I truly think munt was using it in close enough to the right context.
Now that I had to rant and go off topic to explain I will say I usually use swabs to noc agar with spores and haven't had much problems after a few transfers.
Maybe loops can help with spreading the spores away from contam and getting single colonies but I've never tried and didn't think that was so necessary... I have tried with my scalpel a couple times and just seemed to get less growth due to less spores but the colonies were still all mixed together.
Either way you can still get a good organized clean culture with a few transfers.
so back to the point. Cron is right on switching up the nutes for your agar. It helps a lot.
Dog food agar is super cheap and easy just as easy as MEA just isn't as clear but that shouldn't matter anyway.
Just grind up dog food in a coffee grinder or blender and use in place of malt extract.
I use slightly less nutes in my agar when making transfers for faster more rhizo growth. but when transfering to grain, lc, etc you want the regular nute level for strongest and fastest leap off. And spore germination should be the normal recipe too.
so recipe normally is 10g dog food 10g agar 500ml water
I use 8g dog food 10g agar 500ml water
start a few cultures of the same print so you can choose the strongest cleanest from them and discard the questionable ones.
Thanks
And hallelujah I thought I was going mad with my posts and speaking a foreign language.
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Edmunter
Mr



Registered: 05/01/13
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Re: Mexicana grow [Re: Edmunter]
#23773762 - 10/26/16 03:57 PM (7 years, 3 months ago) |
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I just thought id let you all know that me and C10 have ironed out our differences on PM and reccon we will laugh about this on our honeymoon.
Everyone has a bad day and I think we had ours together.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Mexicana grow [Re: Edmunter]
#23774098 - 10/26/16 05:39 PM (7 years, 3 months ago) |
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Edmunter
Mr



Registered: 05/01/13
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It seems worth making a few plates. I have some dog food but havent checked it for nute values. what do you look for in your doggy bits?
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LogicaL Chaos
Ascension Energy & Alien UFOs




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Re: Mexicana grow [Re: Edmunter]
#23775570 - 10/27/16 03:52 AM (7 years, 3 months ago) |
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yikes. glad u guys hugged it out, damn.
Im just here for the mexicana pics
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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I think most basic dog foods will work.
I just look for the cheapest stuff but others have told me even grain free high end stuff works great too.
Blinding Leaf said he uses purina the original flavor green bag with good results and I've tried a few different generic brands and they all seem to work the same.
I think corn is usually the first ingredient.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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I would be surprised if there was a dog food that didn't work.
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Edmunter
Mr



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So with this Psilocybe Mexican I am going to take a wedge or 3 from the slow, clean growing culture and put it on my new media which is dog food and agar 8:10 gram in 500 ml of water.
I have already put a wedge in a small jar of Rye and hope for movement soon.
So how do I shift this media around and why..........e.g. trying to germinate, cloning ect
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tombosley8
Full on... Bossley Baggins



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Re: Mexicana grow [Re: Edmunter]
#23776558 - 10/27/16 12:22 PM (7 years, 3 months ago) |
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You could change back and forth every transfer if you want. I only do about every 2 transfers without any noticeable effects.
Usually spore germination you want slightly softer agar and the full nutrient mix so 10g dog food and slightly less agar so like 9-9.5g agar per 500ml should work depending on your agar.
for transferring wedges to new plates and if that plate is also going to be transferred to another plate then you want slightly less nutes with the regular agar level of 10g. As said 8g of nutes per 500ml water is about as low as you want.
When you plan on inoculating lc, grain, etc with a plate you want to have the full nutrition and agar for strong fast leap off. So 10g agar 10g dog food 500 ml water.
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Edmunter
Mr



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Make sense, thanks.
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blackout


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Re: Mexicana grow [Re: Edmunter]
#23776866 - 10/27/16 02:04 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: Can somebody tell me what the fuk I said that was so mind bendingly confusing?
Nothing was particularly confusing, you have to understand the sometimes bizarre mentality of some people, they pretend to be fucking ignorant cunts in a weird attempt to appear "smart", but more often than not this backfires very badly, as is the case here, they either appear as stupid or just as dicks to those who know they are faking it. Use the ignore function, it's a great feature, if someone is such a cunt that they are pretending to be that stupid you will have a better time on the forum by not seeing their future disingenuous posts, but if the genuinely are that ignorant & stupid you are also better off, so it's really win-win.
I think RR advised on gypsum as an agar additive for stones, along with the coffee already advised.
Edited by blackout (10/27/16 02:09 PM)
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Edmunter
Mr



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Re: Mexicana grow [Re: blackout]
#23777222 - 10/27/16 03:42 PM (7 years, 3 months ago) |
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Ive now made up with C10, he genuinely said he really didn't understand what I was saying. He sounded intelligent and like someone who should get it and I thought he was trolling me or nip picking. He say different and if thats the case then maybe he was confused and not as open with wording as the rest of us and wanted to correct my terminology.
There's no hard feelings and id like to get on with growing stones now. Id like to point out that some of this is not for noobs and my language may not be text book as I like it to be a bit more forum chatty banter rather than strict classroom.
So have a bunch of mushrooms as a peace sign and lets rock!
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23779751 - 10/28/16 10:59 AM (7 years, 3 months ago) |
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Ok Im taking one of the good looking Meicana and Sandose cultures and Im going to transfer them to a 8:10 dog food agar ratio plates. Give their legs a good run and let em loose hoping to transfer back to 10:10 for a few tiger drops into some grains to get the G2G going. In the mean time I am going to make some LI s so I can pp5 a few so I can get some spore prints to give away to anyone who needs them.
Anything sound wrong there?
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dankington
The Stranger




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Re: Mexicana grow [Re: Edmunter]
#23779758 - 10/28/16 11:02 AM (7 years, 3 months ago) |
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just as long as you mean 8gDF, 10gA, 500mL water then you're good
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Edmunter
Mr



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So ive made my second transfer and if this grow nicely on its new home of DF8:A10 I will use 1 plate to add to my grain jars im hoping to fruit. The other I will transfer back to 10:10 with a little coffee and make a few stone making jars.
A few things on my mind. If like other grows I let an MS grow out and pick the favourite mushroom and clone, what are the hard and fast rules on stone producers for this. Is it better to clone the mushroom for the better mushroom flushes and the stones for the stone producers? I suppose its all in the genetics and this kinda makes sense.

While doing all my agar work today my Pan Sandose plate was very fluffy. I took some wedges from this plate and placed on 8:10 media. Can somebody explain why is grew like this. Its was on PADY 10g agar, 10g potato starch, 7g dextrose, 1g yeast
Not the greatest picture but you can see the fluff
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bodhisatta 
Smurf real estate agent


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Re: Mexicana grow [Re: Edmunter]
#23782825 - 10/29/16 02:21 PM (7 years, 3 months ago) |
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if you're cloning for a fruiting strain or a stone strain depends. if you want a fruiting strain of a stone producer clone from a grow. if you want a stone producing just clone a stone that forms on agar.
sometimes it just goes fluffy who cares
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Edmunter
Mr



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Quote:
bodhisatta said: if you want a stone producing just clone a stone that forms on agar.
Never heard of this before. Great advice. So just take a plate, forget about it and hope it produces a stone before a pin........Rocking stuff!
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23782952 - 10/29/16 03:19 PM (7 years, 3 months ago) |
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So interestingly if you made a big enough agar plate you could grow stones str8 from that. Could be a fun experiment.
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oakley
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Re: Mexicana grow [Re: Edmunter] 1
#23787432 - 10/31/16 01:48 AM (7 years, 2 months ago) |
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Quote:
RogerRabbit said: Actually, it's very easy to isolate a super producing sclerotia strain.
Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color. By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.
Now, take each stone and move it to a new dish. Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate. Pick strains which form four or five stones within two weeks and use these for your grain masters. RR
check here for more info
https://www.shroomery.org/forums/showflat.php/Number/22818693#22818693
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Edmunter
Mr



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Re: Mexicana grow [Re: oakley]
#23787493 - 10/31/16 03:23 AM (7 years, 2 months ago) |
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Banging shit Oakley, thanks man!!!
Gunna try this
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23788447 - 10/31/16 12:28 PM (7 years, 2 months ago) |
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This is what I ment by being busy. I still have a few weeks off so I can really crack on.
Edited by Edmunter (10/31/16 12:30 PM)
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tombosley8
Full on... Bossley Baggins



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Re: Mexicana grow [Re: Edmunter]
#23788465 - 10/31/16 12:34 PM (7 years, 2 months ago) |
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hey where'd you find those no pour containers?
been looking everywhere and the only place is big lots so far that has them. love them but I bought out all of what they had and still want more.
Found them on amazon but ridiculously expensive.
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Edmunter
Mr



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They are from the UK and really cheap. A place called home bargains.
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tombosley8
Full on... Bossley Baggins



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Re: Mexicana grow [Re: Edmunter]
#23788559 - 10/31/16 01:09 PM (7 years, 2 months ago) |
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shit
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Edmunter
Mr



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I have a sweet tidy little culture from the original tranfers. I think I may turn it into a LC. I havent made one for years and think it might work well for this.
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23788806 - 10/31/16 02:36 PM (7 years, 2 months ago) |
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They are so cheap I could pack and send to t US if that where ur from? I think about $1.3 for 3, the large ones are £1.69.... $2. Ive never had to throw on away yet.
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Edmunter
Mr



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Quote:
tombosley8 said: hey where'd you find those no pour containers?
been looking everywhere and the only place is big lots so far that has them. love them but I bought out all of what they had and still want more.
Found them on amazon but ridiculously expensive.
Just been to the shop to get some more they are 69 pence for a set of 3 which equates to 84 Cents.
These cost $1.82 each!

Transglobal charge $37 dollars for a 6 kilo parcel.
An option if you ever needed hundreds
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23791183 - 11/01/16 12:18 PM (7 years, 2 months ago) |
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Actually ive done another calculation. To send a Kilo costs $10.54 and you get 30 rounds to a kilo. So for 30 it would cost you $25.20 so altogether it would be $35.74.
I Dont mind sending if thats cost effective for you?
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23791265 - 11/01/16 12:57 PM (7 years, 2 months ago) |
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What kind of mix do these like when making a LC, Any recipes that have been successful for you?
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dankington
The Stranger




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Re: Mexicana grow [Re: Edmunter]
#23791300 - 11/01/16 01:09 PM (7 years, 2 months ago) |
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LC recipes should be like agar recipes, without the agar. Stop bumping so much. You can edit posts.
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Edmunter
Mr



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Yeah sorry about that got a bit excited to find a cheap rate for T. With the LC I was wondering if there was anything specific that would help. i.e. they like cofffee and gypsum so I was thinking of making a 2% mix of Maple syrup, coffee and gypsum.
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dankington
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Re: Mexicana grow [Re: Edmunter]
#23791416 - 11/01/16 01:45 PM (7 years, 2 months ago) |
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I would try LME or potato first. I'd not use coffee or gypsum for an LC, personally.
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bodhisatta 
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or maple syrup
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Edmunter
Mr



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Thanks but ive decided to do a filtered grain soak which has gypsum and coffee in it anyway.
Should be ok?
Might keep some for an experiment on plates too. Can never have too many plates right?
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blackout


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Quote:
dankington said: LC recipes should be like agar recipes, without the agar.
I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.
It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.
Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.
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tombosley8
Full on... Bossley Baggins



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Re: Mexicana grow [Re: blackout]
#23792107 - 11/01/16 05:06 PM (7 years, 2 months ago) |
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I've been doing 1g malt extract per 600ml water.
I thought that is what TL's LC tek calls for...
let me recheck that.. yep so why so low there?
Quote:
TranscendingLife said: - Measure out 400mL of water per pint jar, or 600mL of water per quart jar.
- Measure out .667g of (EL)ME per pint, or 1g per quart.
0.16667% nutrition content
Am I getting that right?
I feel like my leap off is slow maybe too low nutrition but I'm not sure.
0.5% seems way to high though so maybe I'll try more like 0.25%
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Edited by tombosley8 (11/01/16 07:08 PM)
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dankington
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Re: Mexicana grow [Re: blackout]
#23792137 - 11/01/16 05:16 PM (7 years, 2 months ago) |
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Quote:
blackout said:
Quote:
dankington said: LC recipes should be like agar recipes, without the agar.
I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.
It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.
Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.
I apologize for the miscommunication. I meant in regards to the contents of the recipe, not necessarily the ratios. When OP said he wanted to use coffee, gypsum and maple syrup, I thought it was a pretty bad idea.
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Edmunter
Mr



Registered: 05/01/13
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Quote:
dankington said:
Quote:
blackout said:
Quote:
dankington said: LC recipes should be like agar recipes, without the agar.
I have found a lot less sugar works better, as recommended by Workman of sporeworks. I thought he made a typo at first but he confirmed he used 0.1-0.5% sugars in LC, while agar is typically 2-4%.
It still gives plenty of growth, it is a tiny bit saving on materials, but has advantages of being clearer too, I find it less "clumpy" and so easier to draw into syringes. The reasoning given was also that it was not wallowing in its own waste (which I think is the same as what he called hypertonic stress) and closer to being stored in a dilute water solution, which is more suited to long term storage.
Pretty sure he is the only one I saw recommending such low concentrations, I have tried it many times and it works well. Others have indirectly sort of recommended it by using only grain soak water which would also have low levels of sugar & nutrients.
I apologize for the miscommunication. I meant in regards to the contents of the recipe, not necessarily the ratios. When OP said he wanted to use coffee, gypsum and maple syrup, I thought it was a pretty bad idea.
I think I agree,it was a brain fart. However I think the filtered grain water that has gypsum and coffee added may just be ok?
Thoughts?
I dont really need to do this but I havent made a LC since the bad old days and wanted to do it right.
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Spore Ninja
PsychoMycoPhile


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Quote:
filthyknees said: If your goal is sclerotia formation try 10% coffee agar
Ohhh...wicked cool.... gotta try that.
Anyone done coffee and brown rice?
I'm doing Pollocks brown rice cake recipe vs. some precooked brown rice now. I added coffee grounds to some of each to see what happens. They should be sprouting myc tomorrow.
-------------------- I'm interested in Dr. Pollock's work and his (especially older) strains. Anyone have pix of his Shroommobile RV?? PSA: Protonmail offers free, encrypted email hosted in privacy loving Switzerland. Red Phone / Signal offer free encrypted phone calls and texting between users. All of these programs are available free for PC and Android or apple and are available in the respective stores or online by googling the names. Stay safe out there! The Electronic Freedom Foundation's review of text and messaging program's security is here>>> https://www.eff.org/node/82654
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Edmunter
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What causes this 
Im going to make a third transfer away from a potential contamination on the 2nd. Going back to 10:10 A v DG on this one. I have some AA+ and India Orissa that responded really well to the The 8:10 ratio. I have some PE myc just showing up from my cotton bud swipe which I may put on that to kick start is life with me.
The original spore plate has a few brown bits popping up in it. Maybe I should transfer a few of these bits away from it. Pictures would help which I plan to do later.
I am also going to make a MS LC out of one of the first transfers. Im only really doing it to practice for when I get closer to isolation of both kicking stones and kicking fruit cultures.
There are a few people who us LC for exotics, why is that?
Edited by Edmunter (11/03/16 11:43 AM)
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oakley
Stranger

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Re: Mexicana grow [Re: Edmunter]
#23797060 - 11/03/16 09:50 AM (7 years, 2 months ago) |
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that looks like sectoring to me.
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Edmunter
Mr



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Re: Mexicana grow [Re: oakley]
#23797357 - 11/03/16 11:45 AM (7 years, 2 months ago) |
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Quote:
oakley said: that looks like sectoring to me.
Great I thought I may have piggy backed the first contam I saw on plate 1.
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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
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Quote:
tombosley8 said: I've been doing 1g malt extract per 600ml water.
I thought that is what TL's LC tek calls for...
let me recheck that.. yep so why so low there?
Quote:
TranscendingLife said: - Measure out 400mL of water per pint jar, or 600mL of water per quart jar.
- Measure out .667g of (EL)ME per pint, or 1g per quart.
0.16667% nutrition content
Am I getting that right?
Yes that is 0.167%, don't think I have never seen his tek before. Most other teks recommend 2-4%.
This is a clone of one of Aero's stones, which were cloned from commercial stones, which is almost definitely ATL#7

I don't recall seeing any browning or stones on these plates, but Aeros grows were the fastest producing sclerotia I have ever seen. His original thread here https://www.shroomery.org/forums/showflat.php/Number/20833312/fpart/1/vc/1
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Edmunter
Mr



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Re: Mexicana grow [Re: blackout]
#23798025 - 11/03/16 03:17 PM (7 years, 2 months ago) |
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So much info my head is spinning. Its all different, all works and all have pros and cons. Im going to go at it with different teks and compare.
I think I can go for a stone isolate as a separate project and keep MS grows going, clone grows and some brown sector transfers too. Im mean whats stopping me
My goal is get a load of prints and 2 amazing isolates I can slant, one fruiter, one stone producer.
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cronicr



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Re: Mexicana grow [Re: Edmunter]
#23798173 - 11/03/16 03:58 PM (7 years, 2 months ago) |
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Quote:
Edmunter said: So much info my head is spinning. Its all different, all works and all have pros and cons. Im going to go at it with different teks and compare.
I think I can go for a stone isolate as a separate project and keep MS grows going, clone grows and some brown sector transfers too. Im mean whats stopping me
My goal is get a load of prints and 2 amazing isolates I can slant, one fruiter, one stone producer.
For fruiting I simply went with jalisco...Chicon for stones
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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oakley
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Re: Mexicana grow [Re: cronicr]
#23798547 - 11/03/16 06:05 PM (7 years, 2 months ago) |
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Funny thing, chicon was a horrible stone producer when it first came out. if you read any of the many posts about chicon nindo by elfstone you'd realize that the fruits of chicon are supposed to be special for the clarity and beauty of the trip they produce. so fruit chicon perhaps.
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Edmunter
Mr



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Re: Mexicana grow [Re: oakley]
#23799624 - 11/04/16 01:58 AM (7 years, 2 months ago) |
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I think i only have Chicon, but im sure the genetics for both are in there somewhere. Its going to fun looking. Just been to the local stable to get my mix ready.
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23799694 - 11/04/16 03:07 AM (7 years, 2 months ago) |
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Ive been looking at the growing in a bag way of doing things. So you colonise and lay the bag out and press it down so its and inch or so thick the whole way. Stones the size of apples Rogerer Wabit said.
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23800555 - 11/04/16 12:32 PM (7 years, 2 months ago) |
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So ive just done a third transfer from a suspect plate and dropped some genetics in some fltered grain water to make a LC from a clean plate.
I think this might just be where the wedge has moved or a little myc dropped by the main wedge. I dont think its contamination. What do you think?

I have a cleaner looking one that im going to let grow before making a few transfers.
Then I think im good to go on my first grow.
Gunna use some Hpoo rich, dog food, straw, gypsum, rye grass seed kind of a Tubs. Sterilise them, make a cheeky LI and inoc a few for fruiting. Cloning after but thats another story atm, lets get to this point first.
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brickwallnomad
3



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Quote:
c10h12n2o said:
Quote:
Edmunter said: I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you
i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument
if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."
Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" 
if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally
Quote:
c10h12n2o said:
Quote:
Edmunter said: I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you
i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument
if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."
Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" 
if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally
Ok man, how much more childish can you be? Calm down. You're the one that came on this guys thread being a total condescending grammar nazi. Chill out. Just because he's not using the correct "jargon" since you love to put words people say in quotes to make it sound stupid, you jump all down his throat for petty simple stuff. Nobody really cares if you have debate scholarships. I'm a union electrician, who gives a rats ass? Get your panties out of a wad bud
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dankington
The Stranger




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says the optout 
No need to stir that pot, bud. That's been over.
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Edmunter
Mr



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Re: Mexicana grow [Re: Edmunter]
#23803042 - 11/05/16 11:12 AM (7 years, 2 months ago) |
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So this is the original plate and as you can see its has brown areas. As RR mentioned transferring brown strains to get the stones genetics.
Anyone know anything about this and do these look worth trying to you?
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blackout


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Re: Mexicana grow [Re: Edmunter]
#23803271 - 11/05/16 12:35 PM (7 years, 2 months ago) |
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Quote:
Edmunter said: Anyone know anything about this and do these look worth trying to you?

I would certainly give it a try.
Regarding the names, I have a feeling there might be 2 mexicanas available with the same name but were developed from different original specimens. i.e. they were collected in the same region and so have the same name. I am not certain about that at all though, so someone might correct me. I thought I had seen 2 vendors with the same names but different info in the descriptions.
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Edmunter
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Re: Mexicana grow [Re: blackout]
#23828137 - 11/13/16 07:24 AM (7 years, 2 months ago) |
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So I have this small jar of colonised grains from an early plate. Its thin and whispy myc which I think is how it is.
Now, if I wanted to get a small grow from this to get some pins to clone how would I go about it for a speedy and clean grow. Not sure if cased grains does anything for this species. Im not going for quantity just a clean clone.
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Edmunter
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Re: Mexicana grow [Re: Edmunter]
#23828685 - 11/13/16 11:03 AM (7 years, 2 months ago) |
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Found Sunstone beautiful write up on this.
Cased Grain Substrate:
This is the easiest method to case the grains and fruit them as is. I've had OK results by casing RGS. Had great results by casing Rye.
They don't require a special casing layer, I've used pasteurized 50/50, 50/50+, Jiffy Mix and Plantation Seed Starter Mix with equally great results. Make sure that you use a thin (~1/4") layer of your mix. Spread it evenly on the top of the substrate and put it into your fruiting chamber. I've used a SGFC and Greenhouse and they both perform good. Keep high humidity and lots of FAE throughout the whole fruiting. Keep your temperature between 65-80F. I've had them to fruit on constant 65F and constant 80F and anywhere in between. Light temperature of 6500K is preferred (although I experimented with a 8200K light too with good performance as well) with a cycle of 12/12.
Pins should appear within 10-14 days in optimal fruiting conditions.
The whole thread
https://www.shroomery.org/forums/showflat.php/Number/13431948/fpart/all/vc/1
Looks like he puts str8 into fruiting conditions,
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Edmunter
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Re: Mexicana grow [Re: Edmunter]
#23884771 - 12/01/16 02:58 PM (7 years, 1 month ago) |
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First clean culture I like cut into four and used to inoc.............
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Edmunter
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Re: Mexicana grow [Re: Edmunter]
#23895929 - 12/05/16 06:13 AM (7 years, 1 month ago) |
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Ha ha there are the genetics I was after.
 

The '1 pin' written on the top means 1 transfer which is a pin. It helps if I write the amount of transfers per plate im going to make this week so I can make the correct amount of plates.
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PlantManBee
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Re: Mexicana grow [Re: Edmunter]
#23896884 - 12/05/16 01:33 PM (7 years, 1 month ago) |
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Thanks for the invite Edmunter :P De ja vu!:
I'm growing some P. mexicana Jalisco (multi-spore syringe). Going for sclerotia first of course then I'd like to fruit them too. Anyway, I have 11 jars and they seem to be doing well. I used tyvek because this was my first go and it's free. I have a pillow of polyfill and I'll be switching in the future... may even get the fancy filters :P.
Right now i'm wondering about condensation and how much is natural for a successful grow? 9 of 11 are condensation free and 2 are starting to show quite a bit. This is on two of the faster colonizing jars. On the left one of the condensating jars, the right is like the rest. Is this a problem? Oh yeah, inoculation date was 11/2. Thanks! 
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cronicr



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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
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Can you shake jars that produce stones? I was going to wait till I got to bout 25% and then shake to speed things up but im not sure.
I sometimes find that jars with condensation can have something wrong with them and its usually bacteria. This is not gospel but my own experience.
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PlantManBee
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Re: Mexicana grow [Re: Edmunter]
#23896919 - 12/05/16 01:50 PM (7 years, 1 month ago) |
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Quote:
Edmunter said: Can you shake jars that produce stones? I was going to wait till I got to bout 25% and then shake to speed things up but im not sure.
I sometimes find that jars with condensation can have something wrong with them and its usually bacteria. This is not gospel but my own experience.
Yeah, i thought maybe bacteria :/
well, because of the tyvek I'm not wanting to shake them. However, I do have a PC, extra lids, and the polyfill and silicone. I could basically set up to do a G2G transfer but just swap out the lids. Obviously on the ones that aren't already (maybe) contaminated.
Thanks again. Y'all ever try to isolate P. mexicana on cardboard?
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Edmunter
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Never heard of it......
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cronicr



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Re: Mexicana grow [Re: Edmunter]
#23896965 - 12/05/16 02:04 PM (7 years, 1 month ago) |
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I wouldnt shake jars for stones
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PlantManBee
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Re: Mexicana grow [Re: Edmunter]
#23896982 - 12/05/16 02:09 PM (7 years, 1 month ago) |
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lol, it'll likely be my first cloning (for shrooms) attempt. It's pretty selective for fungi IIUC. 
Yeah, i'm trying patience instead of shaking....for the moment :P
https://www.shroomery.org/forums/showflat.php/Number/6225388
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PlantManBee
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So what's likely to happen to happen if that left jar does have a bacterial population? This is the first time I've even seen a fungus grow invitro. Let alone been the grower.
Also would it be worthwhile to swap out the lids on the jars showing no condensation?
Thanks
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Mycolorado
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Don't swap the lids on your jars unless you want to lose them altogether. Condensation shouldn't be an issue.
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PlantManBee
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Thanks! Ill just make better lids for the next round 
EDIT: Damn! super bummed. The two jars with condensation have friggin Trichoderma! Or at least it's some green shit on the top. I reckon I'll pc them and dump them in the woods. So i'm wondering if the condensation is the cause of contam or an effect. or if it matters.
Edited by PlantManBee (12/06/16 07:45 PM)
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Edmunter
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I have some growth from the 2 pins I transferred and need to make a transfer soon. Im going to make a DFA 8:10 to help it along and then transfer to make some more clean cultures.
The LC is looking good so I will inoculate a few jars soon.
The initial jars I inoculated with wedges are grow extremely slowly and wondered if that is normal?
I havent shaken but the myc doesnt seem to want to fly off the agar.
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Edmunter
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Re: Mexicana grow [Re: Edmunter]
#23962103 - 12/28/16 08:02 AM (7 years, 1 month ago) |
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Ive take 3 transfers from the pin plate and another pin from original Spore plate, plus 2 transfers from areas that looked like stones were forming

The LC is now colonised and I am going to Load up 2 50ml syringes and use the rest to pour into some pp5 containers bottle tek style
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fatmatic
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Quote:
PlantManBee said: Thanks for the invite Edmunter :P De ja vu!:
I'm growing some P. mexicana Jalisco (multi-spore syringe). Going for sclerotia first of course then I'd like to fruit them too. Anyway, I have 11 jars and they seem to be doing well. I used tyvek because this was my first go and it's free. I have a pillow of polyfill and I'll be switching in the future... may even get the fancy filters :P.
Right now i'm wondering about condensation and how much is natural for a successful grow? 9 of 11 are condensation free and 2 are starting to show quite a bit. This is on two of the faster colonizing jars. On the left one of the condensating jars, the right is like the rest. Is this a problem? Oh yeah, inoculation date was 11/2. Thanks! 

Hey I just inoculated 7 jars of WBS with Jalisco hoping for stones too but everywhere I read it says they arent prolific stone producers
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Edmunter
Mr



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Re: Mexicana grow [Re: fatmatic]
#23965134 - 12/29/16 10:23 AM (7 years, 1 month ago) |
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Potential stone plates have been placed in the dark as advised by Stamets
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Edmunter
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Re: Mexicana grow [Re: Edmunter]
#23967428 - 12/30/16 07:42 AM (7 years, 1 month ago) |
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Test Jar for the LC has been inoculated.
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blackout


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Re: Mexicana grow [Re: Edmunter]
#23968117 - 12/30/16 12:59 PM (7 years, 1 month ago) |
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Quote:
Edmunter said: Potential stone plates have been placed in the dark as advised by Stamets
in TMC Stamets was saying to grow sclerotia in darkness, but seems he was taking this advise from even older texts. Did you find something specific about growing plates in the dark? or was it in some newer book?
I have seen some reports of them growing better with light, I would certainly not be going out of my way to put them in total darkness.
I know in the vice "truffle brothers" video they claimed to commercially grow them in darkness, this could be following the old advice in TMC or just more cost effective.
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Mycolorado
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Re: Mexicana grow [Re: blackout]
#23968201 - 12/30/16 01:47 PM (7 years, 1 month ago) |
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It's from TMC p. 206. It is indeed based on research done by Heim and Wasson(1958)...at least, that's where I've seen it. My work area is in the basement where it's dark, not totally dark, but dark and I'm getting quite a bit of stone productions on plates. I'm using 3% MEA, so I'm close to the recipe for stones according to the text. Also, I just toss the jars in a closet for darkness.
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enlightenment
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Re: Mexicana grow [Re: blackout]
#23968220 - 12/30/16 01:55 PM (7 years, 1 month ago) |
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Finally I have a Psi. galindoi isolate. I will do a lot of tests regarding sclerotia next year. I could keep some jars in total darkness and compare them with the other jars. Another test is to increase the surface area inside the jar/bag to stimulate sclerotia production in the center of the substrate. Usually there are no 'stones' in the center of the substrate.

 I put these glasses in the center of the bag.
https://www.shroomery.org/forums/showflat.php/Number/23942563#23942563
I open a thread for this soon.
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blackout


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Quote:
Mycolorado said: It's from TMC p. 206.
That is where I saw it before, but it does not specifically talk of petri dishes in darkness, but as it says sclerotia form in darkness in general and on the grains you could presume it too. I just wondered if there was another Stamets text talking about it.
RR said in Stamets seminars he no longer advised to colonise cubensis in darkness, I am not sure if the same would be said for sclerotia.
Quote:
enlightenment said:
 I put these glasses in the center of the bag.
In some old threads I spoke of introducing things like this in bags, if you are searching I used the term "interface" or "interfaces" to describe them. I wondered about just sheets of plastic, like cut up 2L bottles. I am pretty certain some guy (or more) said he tried it and did not find much benefit.
I have also wondered about using actual lasagna sheets, uncooked, so they would remain fairly hard after sterilization, but the myc could digest it if it wanted.
People certainly advise to leave the bags laid out flat to maximise the surface, it should also better help prevent a bag going anaerobic. I do not use bags much anymore so wanted things I could put in larger jars.
I also wondered about the sheets of plastic having a pattern of holes in them, so the myc could make connections to growth on the other side, so it might mimic nature more where it may not hit a perfect blank wall.
Another idea I wanted to try was layered substrate, like having very moist rice at the bottom of a jar with rye/barley/wheat/oats on top, so that the grains would have a plentiful water supply it could draw from if needed.
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Edmunter
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Re: Mexicana grow [Re: blackout]
#23969877 - 12/31/16 03:22 AM (7 years, 30 days ago) |
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Its just something im experimenting with alongside Mycolorado. In fact I should really take one out of the darkness and compare.
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Kenetic
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Re: Mexicana grow [Re: Edmunter]
#23970039 - 12/31/16 06:49 AM (7 years, 30 days ago) |
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-------------------- Todo Cambia    DMT said: Everyone know's me, they just don't know it yet
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PlantManBee
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Re: Mexicana grow [Re: Kenetic]
#23970128 - 12/31/16 08:11 AM (7 years, 30 days ago) |
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I have been wondering about that statement in TMC for the same reason.
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Mycolorado
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Re: Mexicana grow [Re: blackout]
#23970214 - 12/31/16 09:11 AM (7 years, 30 days ago) |
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Here's the quote from TMC...I didn't want to paraphrase: "Optimum temperature for sclerotia production was reported to be at 70-75°F in darknes. Sclerotia on agar media peaked at 4.5% malt concentration. Heim and Wason also found fruitbody production was maximized on agar media when the percentage of malt was balanced to .45%. Nevertheles, sclerotia form best on ryegrass seed incubated in total darkness". It doesn't say anything about petri's in darkness but I think one can draw the conclusion....maybe?
I would definitely experiment with different lighting levels (though they do grow in the dark, sub-surface)but I think more important is the nutrient concentration of the medium. The text suggests high nutes for stones and low nutes for fruit (on malt agar) which makes sense as far as fruitbody formation goes. When it comes down to it, there are most likely a number of factors involved in triggering stone formation...available nutrients, temperature, light (visible/UV/far red), moisture, microbes, etc... None of my very clean transfers formed any stones and even the stones I've transferred have yet to produce more stones(with the exception of one). Again, these are very clean transfers. The mother plates that produced the stones may have had an undetectable bacterial contam that was triggering stones. One of the stone transfers has a small bacterial contam and appears to be forming a stone next to it. Simple observation strongly suggests this may be the most important factor. Pretty sure I've seen real funky jars cranking out stones. Check my thread for pics.
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