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InvisibleMycolorado
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Re: Mexicana grow [Re: blackout]
    #23968201 - 12/30/16 01:47 PM (7 years, 1 month ago)

It's from TMC p. 206.  It is indeed based on research done by Heim and Wasson(1958)...at least, that's where I've seen it.  My work area is in the basement where it's dark, not totally dark, but dark and I'm getting quite a bit of stone productions on plates.  I'm using 3% MEA, so I'm close to the recipe for stones according to the text. Also, I just toss the jars in a closet for darkness.


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Offlineenlightenment
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Re: Mexicana grow [Re: blackout]
    #23968220 - 12/30/16 01:55 PM (7 years, 1 month ago)

Finally I have a Psi. galindoi isolate.
I will do a lot of tests regarding sclerotia next year.
I could keep some jars in total darkness and compare them with the other jars. Another test is to increase the surface area inside the jar/bag to stimulate sclerotia production in the center of the substrate. Usually there are no 'stones' in the center of the substrate.




I put these glasses in the center of the bag.


https://www.shroomery.org/forums/showflat.php/Number/23942563#23942563

I open a thread for this soon.


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Offlineblackout
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Re: Mexicana grow [Re: enlightenment]
    #23968934 - 12/30/16 07:10 PM (7 years, 1 month ago)

Quote:

Mycolorado said:
It's from TMC p. 206.


That is where I saw it before, but it does not specifically talk of petri dishes in darkness, but as it says sclerotia form in darkness in general and on the grains you could presume it too. I just wondered if there was another Stamets text talking about it.

RR said in Stamets seminars he no longer advised to colonise cubensis in darkness, I am not sure if the same would be said for sclerotia.


Quote:

enlightenment said:


I put these glasses in the center of the bag.




In some old threads I spoke of introducing things like this in bags, if you are searching I used the term "interface" or "interfaces" to describe them. I wondered about just sheets of plastic, like cut up 2L bottles. I am pretty certain some guy (or more) said he tried it and did not find much benefit.

I have also wondered about using actual lasagna sheets, uncooked, so they would remain fairly hard after sterilization, but the myc could digest it if it wanted.

People certainly advise to leave the bags laid out flat to maximise the surface, it should also better help prevent a bag going anaerobic. I do not use bags much anymore so wanted things I could put in larger jars.

I also wondered about the sheets of plastic having a pattern of holes in them, so the myc could make connections to growth on the other side, so it might mimic nature more where it may not hit a perfect blank wall.

Another idea I wanted to try was layered substrate, like having very moist rice at the bottom of a jar with rye/barley/wheat/oats on top, so that the grains would have a plentiful water supply it could draw from if needed.


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OfflineEdmunter
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Re: Mexicana grow [Re: blackout]
    #23969877 - 12/31/16 03:22 AM (7 years, 30 days ago)

Its just something im experimenting with alongside Mycolorado.  In fact I should really take one out of the darkness and compare.


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OfflineKenetic
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Re: Mexicana grow [Re: Edmunter]
    #23970039 - 12/31/16 06:49 AM (7 years, 30 days ago)

:popcorn:


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OfflinePlantManBee
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Re: Mexicana grow [Re: Kenetic]
    #23970128 - 12/31/16 08:11 AM (7 years, 30 days ago)

I have been wondering about that statement in TMC for the same reason.


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InvisibleMycolorado
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Re: Mexicana grow [Re: blackout]
    #23970214 - 12/31/16 09:11 AM (7 years, 30 days ago)

Here's the quote from TMC...I didn't want to paraphrase: "Optimum temperature for sclerotia production was reported to be at 70-75°F in darknes.  Sclerotia on agar media peaked at 4.5% malt concentration. Heim and Wason also found fruitbody production was maximized on agar media when the percentage of malt was balanced to .45%.  Nevertheles, sclerotia form best on ryegrass seed incubated in total darkness".  It doesn't say anything about petri's in darkness but I think one can draw the conclusion....maybe?

I would definitely experiment with different lighting levels (though they do grow in the dark, sub-surface)but I think more important is the nutrient concentration of the medium.  The text suggests high nutes for stones and low nutes for fruit (on malt agar) which makes sense as far as fruitbody formation goes.  When it comes down to it, there are most likely a number of factors involved in triggering stone formation...available nutrients, temperature, light (visible/UV/far red), moisture, microbes, etc...
None of my very clean transfers formed any stones and even the stones I've transferred have yet to produce more stones(with the exception of one).  Again, these are very clean transfers.  The mother plates that produced the stones may have had an undetectable bacterial contam that was triggering stones.  One of the stone transfers has a small bacterial contam and appears to be forming a stone next to it.  Simple observation strongly suggests this may be the most important factor.  Pretty sure I've seen real funky jars cranking out stones.  Check my thread for pics.


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Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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