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OfflineEdmunter
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Re: Mexicana grow [Re: blackout]
    #23828137 - 11/13/16 07:24 AM (7 years, 2 months ago)

So I have this small jar of colonised grains from an early plate.  Its thin and whispy myc which I think is how it is. 

Now, if I wanted to get a small grow from this to get some pins to clone how would I go about it for a speedy and clean grow.  Not sure if cased grains does anything for this species. Im not going for quantity just a clean clone.



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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23828685 - 11/13/16 11:03 AM (7 years, 2 months ago)

Found Sunstone beautiful write up on this.

Cased Grain Substrate:

This is the easiest method to case the grains and fruit them as is.
I've had OK results by casing RGS.
Had great results by casing Rye.

   

They don't require a special casing layer, I've used pasteurized 50/50, 50/50+, Jiffy Mix and Plantation Seed Starter Mix with equally great results.
Make sure that you use a thin (~1/4") layer of your mix.
Spread it evenly on the top of the substrate and put it into your fruiting chamber.
I've used a SGFC and Greenhouse and they both perform good.
Keep high humidity and lots of FAE throughout the whole fruiting.
Keep your temperature between 65-80F.
I've had them to fruit on constant 65F and constant 80F and anywhere in between.
Light temperature of 6500K is preferred (although I experimented with a 8200K light
too with good performance as well) with a cycle of 12/12.

Pins should appear within 10-14 days in optimal fruiting conditions.

The whole thread


https://www.shroomery.org/forums/showflat.php/Number/13431948/fpart/all/vc/1


Looks like he puts str8 into fruiting conditions, 


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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23884771 - 12/01/16 02:58 PM (7 years, 1 month ago)

First clean culture I like cut into four and used to inoc.............




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OfflineEdmunter
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Re: Mexicana grow [Re: Edmunter]
    #23895929 - 12/05/16 06:13 AM (7 years, 1 month ago)

Ha ha there are the genetics I was after.




The '1 pin' written on the top means 1 transfer which is a pin.  It helps if I write the amount of transfers per plate im going to make this week so I can make the correct amount of plates.



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Re: Mexicana grow [Re: Edmunter]
    #23896884 - 12/05/16 01:33 PM (7 years, 1 month ago)

Thanks for the invite Edmunter :P  De ja vu!:

I'm growing some P. mexicana Jalisco (multi-spore syringe). Going for sclerotia first of course then I'd like to fruit them too. Anyway, I have 11 jars and they seem to be doing well. I used tyvek because this was my first go and it's free. I have a pillow of polyfill and I'll be switching in the future... may even get the fancy filters :P.

Right now i'm wondering about condensation and how much is natural for a successful grow? 9 of 11 are condensation free and 2 are starting to show quite a bit. This is on two of the faster colonizing jars. On the left one of the condensating jars, the right is like the rest. Is this a problem? Oh yeah, inoculation date was 11/2. Thanks! :stoned:


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Re: Mexicana grow [Re: PlantManBee]
    #23896893 - 12/05/16 01:38 PM (7 years, 1 month ago)

:crondance:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Mexicana grow [Re: PlantManBee]
    #23896898 - 12/05/16 01:41 PM (7 years, 1 month ago)

Can you shake jars that produce stones?  I was going to wait till I got to bout 25% and then shake to speed things up but im not sure.

I sometimes find that jars with condensation can have something wrong with them and its usually bacteria.  This is not gospel but my own experience.


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OfflinePlantManBee
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Re: Mexicana grow [Re: Edmunter]
    #23896919 - 12/05/16 01:50 PM (7 years, 1 month ago)

Quote:

Edmunter said:
Can you shake jars that produce stones?  I was going to wait till I got to bout 25% and then shake to speed things up but im not sure.

I sometimes find that jars with condensation can have something wrong with them and its usually bacteria.  This is not gospel but my own experience.




Yeah, i thought maybe bacteria :/


well, because of the tyvek I'm not wanting to shake them. However, I do have a PC, extra lids, and the polyfill and silicone. I could basically set up to do a G2G transfer but just swap out the lids. :confused: Obviously on the ones that aren't already (maybe) contaminated.

Thanks again. Y'all ever try to isolate P. mexicana on cardboard?


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Re: Mexicana grow [Re: PlantManBee]
    #23896939 - 12/05/16 01:57 PM (7 years, 1 month ago)

Never heard of it......:popcorn:


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Re: Mexicana grow [Re: Edmunter]
    #23896965 - 12/05/16 02:04 PM (7 years, 1 month ago)

I wouldnt shake jars for stones


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Mexicana grow [Re: Edmunter]
    #23896982 - 12/05/16 02:09 PM (7 years, 1 month ago)

lol, it'll likely be my first cloning (for shrooms) attempt. It's pretty selective for fungi IIUC. :stoned:

Yeah, i'm trying
patience instead of shaking....for the moment :P

https://www.shroomery.org/forums/showflat.php/Number/6225388


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OfflinePlantManBee
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Re: Mexicana grow [Re: PlantManBee]
    #23899567 - 12/06/16 10:54 AM (7 years, 1 month ago)

So what's likely to happen to happen if that left jar does have a bacterial population?  This is the first time I've even seen a fungus grow invitro. Let alone been the grower.

Also would it be worthwhile to swap out the lids on the jars showing no condensation?

Thanks :cool:


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InvisibleMycolorado
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Re: Mexicana grow [Re: PlantManBee]
    #23899616 - 12/06/16 11:20 AM (7 years, 1 month ago)

Don't swap the lids on your jars unless you want to lose them altogether.  Condensation shouldn't be an issue.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow


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Re: Mexicana grow [Re: Mycolorado]
    #23899657 - 12/06/16 11:34 AM (7 years, 1 month ago)

Thanks! Ill just make better lids for the next round :stoned:

EDIT: Damn! super bummed. The two jars with condensation have friggin Trichoderma! Or at least it's some green shit on the top. I reckon I'll pc them and dump them in the woods. So i'm wondering if the condensation is the cause of contam or an effect. or if it matters.


Edited by PlantManBee (12/06/16 07:45 PM)


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Re: Mexicana grow [Re: PlantManBee]
    #23918483 - 12/12/16 02:45 AM (7 years, 1 month ago)

I have some growth from the 2 pins I transferred and need to make a transfer soon.  Im going to make a DFA 8:10 to help it along and then transfer to make some more clean cultures. 


The LC is looking good so I will inoculate a few jars soon.

The initial jars I inoculated with wedges are grow extremely slowly and wondered if that is normal?

I havent shaken but the myc doesnt seem to want to fly off the agar.



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Re: Mexicana grow [Re: Edmunter]
    #23962103 - 12/28/16 08:02 AM (7 years, 1 month ago)

Ive take 3 transfers from the pin plate and another pin from original Spore plate, plus 2 transfers from areas that looked like stones were forming



The LC is now colonised and I am going to Load up 2 50ml syringes and use the rest to pour into some pp5 containers bottle tek style


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Offlinefatmatic
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Re: Mexicana grow [Re: PlantManBee]
    #23962291 - 12/28/16 09:46 AM (7 years, 1 month ago)

Quote:

PlantManBee said:
Thanks for the invite Edmunter :P  De ja vu!:

I'm growing some P. mexicana Jalisco (multi-spore syringe). Going for sclerotia first of course then I'd like to fruit them too. Anyway, I have 11 jars and they seem to be doing well. I used tyvek because this was my first go and it's free. I have a pillow of polyfill and I'll be switching in the future... may even get the fancy filters :P.

Right now i'm wondering about condensation and how much is natural for a successful grow? 9 of 11 are condensation free and 2 are starting to show quite a bit. This is on two of the faster colonizing jars. On the left one of the condensating jars, the right is like the rest. Is this a problem? Oh yeah, inoculation date was 11/2. Thanks! :stoned:






Hey I just inoculated 7 jars of WBS with Jalisco hoping for stones too but everywhere I read it says they arent prolific stone producers :frown:


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Re: Mexicana grow [Re: fatmatic]
    #23965134 - 12/29/16 10:23 AM (7 years, 1 month ago)

Potential stone plates have been placed in the dark as advised by Stamets


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Re: Mexicana grow [Re: Edmunter]
    #23967428 - 12/30/16 07:42 AM (7 years, 1 month ago)

Test Jar for the LC has been inoculated.


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Offlineblackout
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Re: Mexicana grow [Re: Edmunter]
    #23968117 - 12/30/16 12:59 PM (7 years, 1 month ago)

Quote:

Edmunter said:
Potential stone plates have been placed in the dark as advised by Stamets




in TMC Stamets was saying to grow sclerotia in darkness, but seems he was taking this advise from even older texts. Did you find something specific about growing plates in the dark? or was it in some newer book?

I have seen some reports of them growing better with light, I would certainly not be going out of my way to put them in total darkness.

I know in the vice "truffle brothers" video they claimed to commercially grow them in darkness, this could be following the old advice in TMC or just more cost effective.


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