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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 10 hours
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Mexicana grow
#23770115 - 10/25/16 01:51 PM (7 years, 3 months ago) |
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Im using an PADY of 10;10;7;1 which works fine for cubensis. However it is a bit slow more Mexicana and im not sure why.
The best growth im getting is from the initial spore swipe. Is it advisable to use a wedge from that to inoculate a jar?
I have loads of wedges growing VERY slowly but im bored and need more jobs...........
Edited by Edmunter (12/12/16 02:37 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mexicana grow [Re: Edmunter]
#23770133 - 10/25/16 01:56 PM (7 years, 3 months ago) |
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im confused by your question, mind clarifying?
what do you mean when you say you have wedges growing? typically, you streak spores to isolate clean growth by transferring it, then keep doing transfers if you want an isolate or more organized culture
this is the way i do it, saves tons of time:

this gives you lots of colonies to choose from, with various levels of strain diversity
then once you have a clean, organized culture, you can do a tiger drop (tic tac toe) to grain for a master or whatever. or you could make a liquid inoculant. but no, you definitely dont want to use the plate which you originally streaked
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Edmunter
Mr



Registered: 05/01/13
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Yep do all that already but never used an original plate.
Why not use it?
I love ur little diagram but I need a loop as my sterilised cotton bud arnt long enough.
I usually swipe, transfer, grow, clone, grow but i have no equipment thats not being used. Its like a factory here and the missus is going nuts. Have a jar of grains and thought why not........ and u can answer that.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Mexicana grow [Re: Edmunter] 1
#23770231 - 10/25/16 02:28 PM (7 years, 3 months ago) |
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The plate you put your spore on the initial plate may have un-germinated contamination that you may inadvertently put into a grain jar. It's best to use a subsequent plate to avoid that
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c10h12n2o
serial dilutor



Registered: 01/21/15
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i still dont understand what you mean by "wedges growing" though? im confused by the context you use the word "wedge" in
cotton for streaking agar? thats crazy, definitely start using something you can flame sterilize. standard lab inoculation loops are the bomb, and dirt cheap, but it is dead simple to make your own as well: all it is is wire made into a loop and twisted around itself, with an optional handle.
it isnt possible to follow the diagram's technique with a swab, since the utensil has to be flame sterilized at 4 different points, and using 4 different swabs is begging for problems
lol many of us can certainly sympathize with trying not to clutter up the lady of the house's kitchen and other areas, ive tried to build some stuff to keep everything contained and out of sight and way as much as possible and generally plan better, and that has helped tremendously
i wouldnt inoculate with a plate that was less than 100% colonized if i could help it (not necessary but speeds things up) and definitely a few transfers from spores. it usually takes a few transfers to get an organized culture and be sure its free from bacterial hobos
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Mexicana grow [Re: Edmunter]
#23770573 - 10/25/16 04:21 PM (7 years, 3 months ago) |
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If your goal is sclerotia formation try 10% coffee agar
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 10 hours
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Quote:
c10h12n2o said:

Let me try to make it simple for you -----
i still dont understand what you mean by "wedges growing" though? im confused by the context you use the word "wedge" in
--------------------------------------------- A wedge is a small piece I cut out from a growing culture and either put in a jar, blend up in a LI or grow out into another culture. So when I say I have wedges growing it means I am growing cultures from wedges....not clones, not spores.....ect
cotton for streaking agar? thats crazy, definitely start using something you can flame sterilize. standard lab inoculation loops are the bomb, and dirt cheap, but it is dead simple to make your own as well: all it is is wire made into a loop and twisted around itself, with an optional handle.
---------------------------------------------- Might seem crazy but if u put cotton buds in silver foil when u sterilize jars they are good to go. Never have a problem.
it isnt possible to follow the diagram's technique with a swab, since the utensil has to be flame sterilized at 4 different points, and using 4 different swabs is begging for problems
lol many of us can certainly sympathize with trying not to clutter up the lady of the house's kitchen and other areas, ive tried to build some stuff to keep everything contained and out of sight and way as much as possible and generally plan better, and that has helped tremendously
i wouldnt inoculate with a plate that was less than 100% colonized if i could help it
------------------------------------- I have 10 different spawn jars growing al sorts of different things, im only curious because ive never thought about doing this before and the wedges/transfers/new cultures are growing slowly. I have over 40 dishes in use atm
(not necessary but speeds things up) and definitely a few transfers from spores. it usually takes a few transfers to get an organized culture and be sure its free from bacterial hobos
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 10 hours
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Quote:
filthyknees said: If your goal is sclerotia formation try 10% coffee agar
Will do
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Mexicana grow [Re: Edmunter]
#23770723 - 10/25/16 05:05 PM (7 years, 3 months ago) |
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what horrible formatting, almost as hard to interpret as your syntax and misuse of jargon 
also take a look at the forum rules. try to use the "edit" button rather than double posting
lol your attempt to be a smartass is quite flaccid, but thanks for "making it simple for me." how bout try making sense in the future, or at least not acting like a dickhole when someone asks you for clarification while answering your question
what you have growing are cultures on plates, not "wedges." you could take a wedge from a culture, or use a wedge from a culture to inoculate anything from another plate to grainspawn (and a thousand other things), but the fact that it is shaped like a wedge doesnt tell us anything about whether it is MS, monoculture, clone, MEA, or anything else
This sentence makes no sense at all, and suggests that you have a poor understanding of what any of these words mean (no offense, you just need to do some reading):
Quote:
So when I say I have wedges growing it means I am growing cultures from wedges....not clones, not spores.....ect
And as far as the cotton, you are entirely missing my point. of course it could work if you sterilize it, but for that matter you could use your finger and it might work, that doesnt make it a good idea. might as well be using a sponge from your sink
the point of streaking a plate with a loop is to have very controlled amounts of tissue or spore transfer and spread out inoculant to form multiple distinguishable colonies. using a swab could smear something around, but flame sterilizing is easier and more suited to the task of streaking plates than pressure cooking q-tips
you are completely misunderstanding lots of vocabulary words and techniques. sterile swabs are usually used to swab the gills of fruits (especially fruits that are tough to get to drop spores), and wrapped in foil to be traded like spore prints. There are several ways to use these spore swabs, but the sloppiest would be to touch agar with it (which would not be good for long term storage of the swab after either). The best way is to simply scrape the spore swab with a flame sterilized inoculation loop and then streak a plate, and transfer clean colonies to new plates (avoiding any visible contamination). Alternatively, you could use a flame sterilized scalpel blade in a pinch: it would be much harder to finesse but would be infinitely better than whatever the hell you are doing with a swab on agar
@bodhi: ......speaking of swabs..... lol im still down to amazon prime you some swabs and foil and whatever else you need, ya greedy bastard
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 10 hours
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I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
Edited by Edmunter (10/26/16 09:56 AM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Mexicana grow [Re: Edmunter]
#23772338 - 10/26/16 05:05 AM (7 years, 3 months ago) |
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Sup munter?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Edmunter
Mr



Registered: 05/01/13
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Last seen: 19 days, 10 hours
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Re: Mexicana grow [Re: cronicr]
#23772465 - 10/26/16 07:16 AM (7 years, 3 months ago) |
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Hey Cron, been a while. Hows the music going bro?
Seems im getting my wording all twisted so nobody understands.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Mexicana grow [Re: Edmunter]
#23773238 - 10/26/16 01:01 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: Im using an PADY of 10;10;7;1 which works fine for cubensis. However it is a bit slow more Mexicana and im not sure why. If they are getting sluggish on agar switch the media up...I find they get lazy fast. The best growth im getting is from the initial spore swipe. Is it advisable to use that as a wedge for a jar?
I have loads of wedges growing VERY slowly but im bored and need more jobs...........
try switch your media up it might simply be getting lazy
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
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Re: Mexicana grow [Re: Edmunter]
#23773253 - 10/26/16 01:06 PM (7 years, 3 months ago) |
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Quote:
Edmunter said: I think you acting like a fucking know it all is a little bit tedious and people dont like it.
I know what a culture is and taking a wedge from a culture and growing it on another plate is exactly that. Until I see a culture I call them wedges. God, I thought this was becoming a prick free zone. Ive seen you be condescending to others, it doesnt help. I think everybody else understood my post except you.
Im way past the point of having to explain myself.
I get your point on the loop but I would only use it twice a year. Sterile cotton buds are just as a easy.
I think we need to start again
Greetings Im Edmunter how do you do?
um, maybe calm down a little bit? if you quit looking for something to take personal and get offended by youd probably agree with me, or at least make sense. you clearly havent heard a word anyone has said to you
i find it hilarious how many cry-bullies we have been having in mush cult lately, that ask misguided misworded questions and then talk down to the people who take the time to answer their question and offer constructive criticism, and then act all butthurt and mistreated when someone puts them in their place with a well-constructed argument
if you cant handle criticism or feedback, maybe dont ask a question... or grow thicker skin so you can have an adult conversation without finding everything "condescending."
Also, maybe dont pick an argument with the guy who's debate scholarships paid his way through college if you cant back it up with anything more than calling someone a "know it all" in the same breath as saying "im way past the point of having to explain myself" 
if you would drop the animosity/arrogance you would probably agree with me, or at least not take it so personally
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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You might think your long winded garbage posts make you a winner here but you are far from right knock the crap off.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mexicana grow [Re: cronicr]
#23773382 - 10/26/16 01:48 PM (7 years, 3 months ago) |
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is there a game going on? i was not aware some people type/think faster than others
some people will always find something to get offended by, but others actually come here to read and learn, and appreciate concise information. still hard for me to understand how people get offended by it though
not sure what "garbage posts" you are referring to, i think i answered his question pretty effectively, and clarified the vocab for anyone reading
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (10/26/16 01:58 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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The long winded five paragraph off topic crap...
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Mexicana grow [Re: cronicr]
#23773455 - 10/26/16 02:15 PM (7 years, 3 months ago) |
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lol my posts explaining the problem with streaking with a q-tip and offering a better suggestion? or the one where i reminded about not double posting? the one where i explained why OPs phrasing was confusing, and clarified for the sake of anyone reading who is actually trying to help or learn something? or the one where i reiterated that cultivation advice is not personal and shouldn't offend anyone?
i dare say ALL of my posts in this thread are more on topic and less crap than:
Quote:
cronicr said: Sup munter?

but what do i know, apparently you think its worth the ban warning you just sent me. I was not aware that telling someone not to get offended by cultivation advice was against the rules, but i guess its your playground, whatever makes you feel better
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
Edited by c10h12n2o (10/26/16 02:27 PM)
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 10 hours
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Is this all because I said wedges instead of cultures that I know nothing?
You are the first person to be a proper insulting dick here to me.
Im a humble guy trying to get some answers from a community of pretty cool people.
You need to calm down and stop trying to outsmart people.
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cronicr



Registered: 08/07/11
Posts: 61,436
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Last seen: 2 years, 9 days
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Re: Mexicana grow [Re: Edmunter] 1
#23773513 - 10/26/16 02:37 PM (7 years, 3 months ago) |
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Well now he is taking a break back on topic people..munter try switch your media up it helps with them.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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