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OfflineColonelAngus
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Pac Man on Agar - What to do next? [PICS]
    #23769357 - 10/25/16 09:28 AM (7 years, 3 months ago)

This is my first try with agar, and I'm on my 2nd transfer.

I've got this pac man shape on what otherwise looks almost like it would be a mono culture.

Does the odd shape tell me that something, maybe bacteria is there in the 'blank' section?

Also, if I do have any contamination on a plate like this, but let the rest grow out, can I use the clean sections to inoculate grains, or should I just transfer clean sections to new plates and use those?



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OfflineColonelAngus
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Re: Pac Man on Agar - What to do next? [PICS] [Re: ColonelAngus]
    #23792920 - 11/01/16 08:35 PM (7 years, 2 months ago)

Well, I didn't get any replies after almost 50 views. A few days ago,  I went ahead and took several small snips from the back of pac-man's head and put them in new dishes. Everything looks clean so far.

Pac Man is more than double the size in the photo now. I'd like to use it to noc some small jars and test.

If anyone wants to comment on my noobery, feel free. :confused:


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Invisibleazur
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Re: Pac Man on Agar - What to do next? [PICS] [Re: ColonelAngus]
    #23792928 - 11/01/16 08:37 PM (7 years, 2 months ago)

Pac man's mold is mold.
You did the right thing. Do not use pac man to inoculate jars.


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineColonelAngus
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Re: Pac Man on Agar - What to do next? [PICS] [Re: azur]
    #23793102 - 11/01/16 09:24 PM (7 years, 2 months ago)

Quote:

azur said:
Pac man's mold is mold.
You did the right thing. Do not use pac man to inoculate jars.





A reply! Thanks Azur. So the mouth is mold, but I used the back of the head to isolate what looked clean.

If the new plates grow out evenly, I'll just use those.

But also, am I taking a big risk if I want to use a few chunks from pac-man's head to noc some 1/2 pt jars and see if they can out-run any contams? Or just chuck the plate and pray for my new ones?


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Invisibleazur
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Re: Pac Man on Agar - What to do next? [PICS] [Re: ColonelAngus]
    #23793176 - 11/01/16 09:47 PM (7 years, 2 months ago)

Don't chuck it. Take more transfers.
Do not use for jars though.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblePastywhyteMDiscord
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Re: Pac Man on Agar - What to do next? [PICS] [Re: azur]
    #23793182 - 11/01/16 09:49 PM (7 years, 2 months ago)

Spotting shit like that and transferring away from it is what agar is all about.


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Invisiblemorty422
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Re: Pac Man on Agar - What to do next? [PICS] [Re: Pastywhyte]
    #23793197 - 11/01/16 09:56 PM (7 years, 2 months ago)



Pacman's got a dirty mouth!

:rofl:


Edited by morty422 (11/01/16 10:38 PM)


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Invisibledankington
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Re: Pac Man on Agar - What to do next? [PICS] [Re: morty422]
    #23793200 - 11/01/16 09:58 PM (7 years, 2 months ago)

:astonishedmustache: what the shit are you talking about? Real talk goin' on here, son.


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OfflineSloppyJoseph
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Re: Pac Man on Agar - What to do next? [PICS] [Re: dankington]
    #23793258 - 11/01/16 10:17 PM (7 years, 2 months ago)

Easy tiger.

Let's see those new plates soon OP


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Invisiblemorty422
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Re: Pac Man on Agar - What to do next? [PICS] [Re: dankington]
    #23793307 - 11/01/16 10:37 PM (7 years, 2 months ago)

Quote:

dankington said:
:astonishedmustache: what the shit are you talking about? Real talk goin' on here, son.




I quoted Pasty -but my comment was not intended to 'dis' him.

More to make a joke about pacman having a dirty mouth.
I should have just replied without quoting.
I'll edit it.

I'm in total agreement that transferring the opposite side (9 o'clock) is the best way to move forward.

:cheers:


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Invisibleazur
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Re: Pac Man on Agar - What to do next? [PICS] [Re: morty422]
    #23793364 - 11/01/16 11:03 PM (7 years, 2 months ago)

You're a teapot? Is that some millennial word for tranny/freak?


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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OfflineColonelAngus
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Re: Pac Man on Agar - What to do next? [PICS] [Re: SloppyJoseph]
    #23802955 - 11/05/16 10:35 AM (7 years, 2 months ago)

Quote:

SloppyJoseph said:
Easy tiger.

Let's see those new plates soon OP




Here's the latest transfer. Looking pretty clean so far



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OfflineSloppyJoseph
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Re: Pac Man on Agar - What to do next? [PICS] [Re: ColonelAngus]
    #23806234 - 11/06/16 11:35 AM (7 years, 2 months ago)

Yup looks good dude


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