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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
How bad did I mess up * 1
    #23768858 - 10/25/16 02:21 AM (7 years, 4 months ago)

Hey everyone I think I messed up bad...

So I did some transfer from some of my ms pasty plates in a sab.
A lot harder than I thought it would be. I started off with 1 qt of wbs put in 4 wedges and everything was sterile except i think my knife was too hot it melted the agar and I heard it almost sizzle. I cut around myc and made sure I saw white on the transfer (I heated my knife red hot before every transfer ) do you think this is fucked?

I also did 5 transfers to new plates I prepared  same problem with the knife except for one or two of the transfers I feel like I totally messed everything up and like a failure :frown:

Will I be able to get anything out of this ? I still have two original unopened pasty plates I'm thinking on tiger dropping them. Any tips on transferring would gladly be appreciated.

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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23768862 - 10/25/16 02:25 AM (7 years, 4 months ago)

A little sizzle aint that bad.  Tic tac toe tiger drop ftw.


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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Offlinemrmazdarx9
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23768864 - 10/25/16 02:27 AM (7 years, 4 months ago)

Its probably ok, you should dip the knife in the agar away from your myc a few times to cool it before cutting out the wedges in future. If you look at others plates there always little score marks where they cool their blades


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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: wtfcrazymofo]
    #23768870 - 10/25/16 02:33 AM (7 years, 4 months ago)

I was in there for 50 mins haha so the sizzle was fine ?
Also I hear how ur not supposed to have your gloved hand over open media I may have totally fucked this up in every way  :holyshit:

I feel very bad about this hopefully I can get something out of this?
I wish I had someone to show me in person lol I have a whole shitload of spores though just gotta keep trying till I get it right :mushroom2:

I actually did do that I dipped my blade into the receiving agar tray and once with uncolonized agar from the transfer tray but not for every transfer :rasta:

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Offlinewtfcrazymofo
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23768894 - 10/25/16 03:01 AM (7 years, 4 months ago)

The mycelium don't like to be burned, but it just takes a little longer for the recovery.  Never go over the media.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: wtfcrazymofo]
    #23768899 - 10/25/16 03:07 AM (7 years, 4 months ago)

Yeah thanks I know where I messed up and hopefully can work on these mistakes ahhh any tips for not going over media

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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23769107 - 10/25/16 06:49 AM (7 years, 4 months ago)

.


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:08 PM)

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: amidogen]
    #23769198 - 10/25/16 08:01 AM (7 years, 4 months ago)

I've read and watched these I just need more practice I guess

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OfflineLogicaL ChaosM
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23769268 - 10/25/16 08:47 AM (7 years, 4 months ago)

A super hot knife shouldnt kill ALL the mycelium, so i wouldnt worry too much.

But yah, lesson learned right? Wait till its not glowing red next time you cut :wink:


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Offlinejust_curious
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Re: How bad did I mess up [Re: LogicaL Chaos] * 1
    #23769398 - 10/25/16 09:45 AM (7 years, 4 months ago)

I always cool the blade in the receiving plate, not the donor plate. Dont feel too bad. Every time I do SAB in prep for a new grow, I always feel like it went terrible. This is especially true when I'm doing transfers and the wedge gets stuck on my blade. No matter what, I can't get it off. This is where it gets sketchy. I take my alcohol towel and wipe down my finger, then gently push the wedge off my blade allowing it to break free and land on the agar. Every time I have to do that I'm like "fuck! Well, this plate is fucked!!" But it always turns out to be fine. I'm sure everybody here is a perfectionist to some degree, otherwise we probably wouldn't have gotten into this hobby.

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: just_curious]
    #23770694 - 10/25/16 04:56 PM (7 years, 4 months ago)

It wasn't glowing red just hot and damn yeah I had an issue with it getting stuck on my blade I really need an xacto I just prep'd another grain jar to do an transfer tonight hopefully I can do it faster this time

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OfflineX2QE
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Registered: 07/17/16
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23770778 - 10/25/16 05:28 PM (7 years, 4 months ago)

I wouldn't worry too much about cutting into the myc when the blade is hot. If I recall correctly, in Paul Staments book about gourmet mushroom cultivation he says to cut right into the mycelium when the blade is hot. That is what I have been doing and I can see the myc leaping off onto grain in ~24 hours. Can't be hurting it that much. :shrug:

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: X2QE]
    #23770816 - 10/25/16 05:43 PM (7 years, 4 months ago)

Awesome I just have to work on not going over open media then thanks

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InvisibleMunchauzen
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23770828 - 10/25/16 05:47 PM (7 years, 4 months ago)

In my video, I do show cooling the blade off in the agar.

I do not do this anymore. I go in red hot. No problems. :thumbup:

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OfflineGreg
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Re: How bad did I mess up [Re: Munchauzen]
    #23770843 - 10/25/16 05:51 PM (7 years, 4 months ago)

Yeah, unless I'm doing tiny tiny wedges/transfers (so small the heat will melt them), I just cut while the blade is red hot.

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: Greg]
    #23771085 - 10/25/16 07:03 PM (7 years, 4 months ago)

Oh well ok thanks guys im gunna try to do a Tictactoe drop tonight wish me luck

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OfflineX2QE
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23771095 - 10/25/16 07:05 PM (7 years, 4 months ago)

:goodluck:

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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: X2QE]
    #23771233 - 10/25/16 07:42 PM (7 years, 4 months ago)

I might just go with the blenderless LI tech I don't see any contams no bacteria do you think I'll be fine

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OfflineMushierage
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Re: How bad did I mess up [Re: Hallucyjunkie99]
    #23771250 - 10/25/16 07:49 PM (7 years, 4 months ago)

I wouldn't complicate things any more than you need to.  Just practice your agar work.  LI isn't really that great if you already have a few plates grown out and clean.  Just use that to inoculate, and you'll generally have clean spawn.  LI just adds in an extra vector for contamination (water) and isn't really worth it unless you intend on doing an entire quart of LI that you'll use to inoculate a shit ton of jars with.  Same with LC.  Worthless and adds in extra contam vectors and not worth it unless you intend to produce a shitload and inoculate a lot of grain at once.


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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: How bad did I mess up [Re: Mushierage]
    #23771260 - 10/25/16 07:52 PM (7 years, 4 months ago)

Ah I see I'll keep it in mind for later though when I need to noc up like 6-8 jars

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