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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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How bad did I mess up 1
#23768858 - 10/25/16 02:21 AM (7 years, 3 months ago) |
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Hey everyone I think I messed up bad...
So I did some transfer from some of my ms pasty plates in a sab. A lot harder than I thought it would be. I started off with 1 qt of wbs put in 4 wedges and everything was sterile except i think my knife was too hot it melted the agar and I heard it almost sizzle. I cut around myc and made sure I saw white on the transfer (I heated my knife red hot before every transfer ) do you think this is fucked?
I also did 5 transfers to new plates I prepared same problem with the knife except for one or two of the transfers I feel like I totally messed everything up and like a failure 
Will I be able to get anything out of this ? I still have two original unopened pasty plates I'm thinking on tiger dropping them. Any tips on transferring would gladly be appreciated.
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
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A little sizzle aint that bad. Tic tac toe tiger drop ftw.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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mrmazdarx9
Pffffttt


Registered: 05/15/16
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Its probably ok, you should dip the knife in the agar away from your myc a few times to cool it before cutting out the wedges in future. If you look at others plates there always little score marks where they cool their blades
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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I was in there for 50 mins haha so the sizzle was fine ? Also I hear how ur not supposed to have your gloved hand over open media I may have totally fucked this up in every way
I feel very bad about this hopefully I can get something out of this? I wish I had someone to show me in person lol I have a whole shitload of spores though just gotta keep trying till I get it right 
I actually did do that I dipped my blade into the receiving agar tray and once with uncolonized agar from the transfer tray but not for every transfer
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 14 hours, 45 minutes
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The mycelium don't like to be burned, but it just takes a little longer for the recovery. Never go over the media.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Yeah thanks I know where I messed up and hopefully can work on these mistakes ahhh any tips for not going over media
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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.
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Edited by amidogen (04/19/18 12:08 PM)
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: amidogen]
#23769198 - 10/25/16 08:01 AM (7 years, 3 months ago) |
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I've read and watched these I just need more practice I guess
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,379
Loc: The Inexpressible...
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A super hot knife shouldnt kill ALL the mycelium, so i wouldnt worry too much.
But yah, lesson learned right? Wait till its not glowing red next time you cut
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just_curious
Cultivator



Registered: 03/15/14
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I always cool the blade in the receiving plate, not the donor plate. Dont feel too bad. Every time I do SAB in prep for a new grow, I always feel like it went terrible. This is especially true when I'm doing transfers and the wedge gets stuck on my blade. No matter what, I can't get it off. This is where it gets sketchy. I take my alcohol towel and wipe down my finger, then gently push the wedge off my blade allowing it to break free and land on the agar. Every time I have to do that I'm like "fuck! Well, this plate is fucked!!" But it always turns out to be fine. I'm sure everybody here is a perfectionist to some degree, otherwise we probably wouldn't have gotten into this hobby.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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It wasn't glowing red just hot and damn yeah I had an issue with it getting stuck on my blade I really need an xacto I just prep'd another grain jar to do an transfer tonight hopefully I can do it faster this time
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X2QE
:(){ :|:& };:

Registered: 07/17/16
Posts: 182
Loc: Non-Observable Universe
Last seen: 6 years, 9 months
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I wouldn't worry too much about cutting into the myc when the blade is hot. If I recall correctly, in Paul Staments book about gourmet mushroom cultivation he says to cut right into the mycelium when the blade is hot. That is what I have been doing and I can see the myc leaping off onto grain in ~24 hours. Can't be hurting it that much.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: X2QE]
#23770816 - 10/25/16 05:43 PM (7 years, 3 months ago) |
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Awesome I just have to work on not going over open media then thanks
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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In my video, I do show cooling the blade off in the agar.
I do not do this anymore. I go in red hot. No problems.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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Re: How bad did I mess up [Re: Munchauzen]
#23770843 - 10/25/16 05:51 PM (7 years, 3 months ago) |
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Yeah, unless I'm doing tiny tiny wedges/transfers (so small the heat will melt them), I just cut while the blade is red hot.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: Greg]
#23771085 - 10/25/16 07:03 PM (7 years, 3 months ago) |
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Oh well ok thanks guys im gunna try to do a Tictactoe drop tonight wish me luck
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X2QE
:(){ :|:& };:

Registered: 07/17/16
Posts: 182
Loc: Non-Observable Universe
Last seen: 6 years, 9 months
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: X2QE]
#23771233 - 10/25/16 07:42 PM (7 years, 3 months ago) |
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I might just go with the blenderless LI tech I don't see any contams no bacteria do you think I'll be fine
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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I wouldn't complicate things any more than you need to. Just practice your agar work. LI isn't really that great if you already have a few plates grown out and clean. Just use that to inoculate, and you'll generally have clean spawn. LI just adds in an extra vector for contamination (water) and isn't really worth it unless you intend on doing an entire quart of LI that you'll use to inoculate a shit ton of jars with. Same with LC. Worthless and adds in extra contam vectors and not worth it unless you intend to produce a shitload and inoculate a lot of grain at once.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: Mushierage]
#23771260 - 10/25/16 07:52 PM (7 years, 3 months ago) |
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Ah I see I'll keep it in mind for later though when I need to noc up like 6-8 jars
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X2QE
:(){ :|:& };:

Registered: 07/17/16
Posts: 182
Loc: Non-Observable Universe
Last seen: 6 years, 9 months
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Re: How bad did I mess up [Re: Mushierage]
#23771468 - 10/25/16 08:57 PM (7 years, 3 months ago) |
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Quote:
Mushierage said: I wouldn't complicate things any more than you need to. Just practice your agar work. LI isn't really that great if you already have a few plates grown out and clean. Just use that to inoculate, and you'll generally have clean spawn. LI just adds in an extra vector for contamination (water) and isn't really worth it unless you intend on doing an entire quart of LI that you'll use to inoculate a shit ton of jars with. Same with LC. Worthless and adds in extra contam vectors and not worth it unless you intend to produce a shitload and inoculate a lot of grain at once.

Just make transfers to new plates. If you are only making 7 jars. You also don't need to do drop the whole plate into one jar. You could inoculate each jar with a single wedge from one plate if you wanted.
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: How bad did I mess up [Re: X2QE]
#23771478 - 10/25/16 09:00 PM (7 years, 3 months ago) |
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I know not to drop the whole plate in I'm doing 4-6 wedges per grain jar and shaking them I just thought IL would be quicker for me but I'm not going to due to contam vectors thank you everyone
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