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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 6 months, 22 days
a little agar work(newb to it)
    #23768181 - 10/24/16 08:37 PM (7 years, 4 months ago)

so i know these look horrible, im trying to get my feet wet in agar.
all work is done in a SAB. and i know where i fucked up with the condensation on the lid. ill fix it next go at pouring plates.

any tips, advice or help will be greatly appreciated.


so this one looks like trich, i think. i made a test LC with the syringe and and then a test grain jar and it got trich quick so i dumped it. there was one spot that looked semi clean so i transfered it to a new agar plate.


so this one looks like black mold and some bacteria. i took three wedges and xfered to three different plates trying to get at least one clean one.


straight up bacteria?


straight up trich or black mold?


thanks guys! the other agar plates i have look pretty good, albeit there are several mycal growths so its gonna be a bitch getting a mono strain but thats better than contams!!

let me know what you think.

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OfflineTh3Issu3
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Registered: 12/30/14
Posts: 671
Loc: fucktown
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Re: a little agar work(newb to it) [Re: numnum59]
    #23768232 - 10/24/16 08:48 PM (7 years, 4 months ago)

Toss all that shit dude. If you know where you fucked up then good, that's how you learn. If I were to guess tho, you touched the agar.

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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 6 months, 22 days
Re: a little agar work(newb to it) [Re: Th3Issu3]
    #23768267 - 10/24/16 08:59 PM (7 years, 4 months ago)

Quote:

Th3Issu3 said:
Toss all that shit dude. If you know where you fucked up then good, that's how you learn. If I were to guess tho, you touched the agar.





Thats all goin in the garbage, whats the stealthiest way to dispose of it. Also,  im pretty sure i didnt touch it. I watched RRs video and another guy on here that showed how to do it

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OfflineTh3Issu3
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Registered: 12/30/14
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Re: a little agar work(newb to it) [Re: numnum59]
    #23768288 - 10/24/16 09:05 PM (7 years, 4 months ago)

Take a spoon, scrape that shot out in the toilet and flush it.

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InvisiblePirateSwazey
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Registered: 12/12/12
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Re: a little agar work(newb to it) [Re: Th3Issu3]
    #23768639 - 10/24/16 11:33 PM (7 years, 4 months ago)

Your plate #3 looks okay from where you took your transfers.

If you stack your plates when you pour them and let them cool like that, it will help reduce condensation. Also keeping them at a stable temperature works miracles. If you think the temp fluctuates too much at your place, putting them in an insulated cooler or lunchbox will help.

:goodluck:


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Invisibleazur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: a little agar work(newb to it) [Re: Th3Issu3]
    #23768663 - 10/24/16 11:46 PM (7 years, 4 months ago)

Quote:

Th3Issu3 said:
Take a spoon, scrape that shot out in the toilet and flush it.



That's is horrible advice.
Nothing goes in a toilet except piss, shit, and toilet paper.
I had to replace all my sewage lines because some nasty fucks before me thought it was cool to put whatever down the shitter.


--------------------


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Offlinewtfcrazymofo
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Registered: 07/26/15
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Re: a little agar work(newb to it) [Re: azur]
    #23768868 - 10/25/16 02:31 AM (7 years, 4 months ago)

burn it, or bag it up and throw it in a grade school dumpster.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922



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Offlinemrmazdarx9
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Registered: 05/15/16
Posts: 9,796
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Re: a little agar work(newb to it) [Re: wtfcrazymofo]
    #23768873 - 10/25/16 02:35 AM (7 years, 4 months ago)

I just chuck mine in the outside bin with all the other moldy food, not that I ever make bad plates:biggrin:


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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Re: a little agar work(newb to it) [Re: wtfcrazymofo]
    #23768874 - 10/25/16 02:39 AM (7 years, 4 months ago)

Quote:

wtfcrazymofo said:
burn it, or bag it up and throw it in a grade school dumpster.



Lol :dumblol:

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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 6 months, 22 days
Re: a little agar work(newb to it) [Re: Hallucyjunkie99]
    #23769092 - 10/25/16 06:38 AM (7 years, 4 months ago)

Is there any way to clean up syringe #1? It seems only trich sprouts from it.

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OfflineTh3Issu3
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Registered: 12/30/14
Posts: 671
Loc: fucktown
Last seen: 2 months, 23 days
Re: a little agar work(newb to it) [Re: azur]
    #23769157 - 10/25/16 07:26 AM (7 years, 4 months ago)

Quote:

azur said:
Quote:

Th3Issu3 said:
Take a spoon, scrape that shot out in the toilet and flush it.



That's is horrible advice.
Nothing goes in a toilet except piss, shit, and toilet paper.
I had to replace all my sewage lines because some nasty fucks before me thought it was cool to put whatever down the shitter.





Oh man! i toss everything down the crapper! it mite of been me who was living there before you brotha!

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Offlinesandy_vag
Keep Dreaming
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Registered: 06/14/16
Posts: 112
Last seen: 5 years, 9 months
Re: a little agar work(newb to it) [Re: numnum59]
    #23769195 - 10/25/16 07:58 AM (7 years, 4 months ago)

Quote:

numnum59 said:
Is there any way to clean up syringe #1? It seems only trich sprouts from it.




Only way I can say is to keep trying. Smallest drop possible on the plates, spread out as much as possible. Maybe in 50 plates you can get a couple of clean germinations. Really depends just how many contam spores are in the syringe. It's gonna be hard because trich mycelium looks a lot like cube mycelium at first until it sporulates.

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Offlinenumnum59
Pro-Am Mycologist


Registered: 12/07/11
Posts: 1,189
Last seen: 6 months, 22 days
Re: a little agar work(newb to it) [Re: sandy_vag]
    #23770890 - 10/25/16 06:05 PM (7 years, 4 months ago)

Quote:

sandy_vag said:
Quote:

numnum59 said:
Is there any way to clean up syringe #1? It seems only trich sprouts from it.




Only way I can say is to keep trying. Smallest drop possible on the plates, spread out as much as possible. Maybe in 50 plates you can get a couple of clean germinations. Really depends just how many contam spores are in the syringe. It's gonna be hard because trich mycelium looks a lot like cube mycelium at first until it sporulates.




Indeed it does

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