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Medhead
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Registered: 10/24/16
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Last seen: 7 years, 12 days
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Ganoderma Lucidum LC contam. Or is it?
#23767683 - 10/24/16 05:56 PM (7 years, 3 months ago) |
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I'm very new at this. So there is this white thin coating beginning to form in top of my LC. Any idea what it could be? Also, it sticks to the side of the jar if I turn it sideways.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23767707 - 10/24/16 06:03 PM (7 years, 3 months ago) |
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Hard to tell, but that is the big down side of LC, your going to have to put that on agar anyways to see if it's good or not.
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
#23767810 - 10/24/16 06:33 PM (7 years, 3 months ago) |
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I was putting off agar work for now but, I may need to make that my next step. I've got to put a filter on my glovebox and get a fan and I should be ready to try some agar work.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23768262 - 10/24/16 08:58 PM (7 years, 3 months ago) |
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No, don't put a filter on a glove box. What you want to build is SAB (still air box), these work on the prinsible of "still air". It's just a tote turned upside down with two holes for your arms, don't be discouraged about doing agar work in one, I and many others have poured hundreds of dishes in SABS and they work great. Where did you get the LC? If it was started in open air than it is more than likely contaminated.
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Medhead
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Registered: 10/24/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
#23769040 - 10/25/16 06:02 AM (7 years, 3 months ago) |
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I got the LC syringes from Peter McCoy. He gave all of us some cultures as part of the radical mycology seminar that he hosts.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23770322 - 10/25/16 02:59 PM (7 years, 3 months ago) |
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Quote:
Medhead said: I got the LC syringes from Peter McCoy. He gave all of us some cultures as part of the radical mycology seminar that he hosts.
Huh, well if he's a big deal you should probably have a good culture, however I find it weird that he opted to give everyone LC syringes instead of plates or slants, especially since the novice or even expert could tell if an LC is good or not with out testing it. That could totally be good though, I have never made Reishi LC but I have heard that it makes a mat of mycelium on the top of the liquid, in fact I have read that there are companies in asia that culture reishi myc in huge vats of liquid culture and harvest the mycelium from the top since it has medical properties as well.
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Greg
always learning




Registered: 10/28/15
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
#23770480 - 10/25/16 03:51 PM (7 years, 3 months ago) |
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My reishi LC has never looked like that when I have made it before. That stuff looks really gross. Mine always looks clear-yellow with bits of myc floating in it like all other types of LC. I do regularly agitate and mix my LCs when I make them though.
Unfortunately I don't have any on hand so I can't get photos.
Definitely test that on agar before you take it to grain or anything else.
Edit: This is what red reishi looks like on agar by the way:
Edited by Greg (10/25/16 05:13 PM)
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
#23770776 - 10/25/16 05:28 PM (7 years, 3 months ago) |
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He did say that he prefers liquid cultures but, for him there is a time and situation for both. He also talked about the advantages and disadvantages of both LC and agar. One of the biggest that I remember off hand is that one has a better chance to save some of the mycelium if there is some contamination but he does go into detail more in his book.
Regarding what you said specifically about Reishi LC, I haven't heard that mentioned before, I'm going look into it. Thanks for responding to my post.
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23770790 - 10/25/16 05:31 PM (7 years, 3 months ago) |
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The liquid has a yellow color. Is that what you are referring to or are you just talking about the white stuff on top?
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Greg
always learning




Registered: 10/28/15
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23770806 - 10/25/16 05:36 PM (7 years, 3 months ago) |
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No, I was describing what my healthy reishi LC has looked like in the past. Yours looks murky/cloudy, I'm not sure what that stuff is on top (doesn't look like healthy myc) It doesn't look like any healthy LC I've ever seen.
Still, it's worth testing on agar.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
#23770827 - 10/25/16 05:46 PM (7 years, 3 months ago) |
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I agree with him there is a time and place for LC, though I had bad experience when I was newer cultivator with it. When I am in hurry to do large batches of grain I utilize Liquid inoculation by blending colonized agar dishes into sterile water and pour into bags, though I'm sure a healthy LC may have a faster leap off time than that.
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Medhead
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Registered: 10/24/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
#23770932 - 10/25/16 06:20 PM (7 years, 3 months ago) |
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Thanks. Yeah I agree it does look murkey. The millet has turned a little darker as well. I have some HE, TV, and LE and none of those look like the GL. I guess I'm about to get some plates and cook some agar up.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23771243 - 10/25/16 07:45 PM (7 years, 3 months ago) |
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Good luck dude Keep us posted and let us know how it goes.
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
#23781021 - 10/28/16 07:20 PM (7 years, 3 months ago) |
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Dang it! It's mold! I tilted the jar over to pull out some LC and there are green specks stuck to the wall of the jar. Is it possible to inoculate some agar with it and allow it grow then, take a clean section of mycelium from the plate?
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Greg
always learning




Registered: 10/28/15
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23781030 - 10/28/16 07:22 PM (7 years, 3 months ago) |
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It's possible but I wouldn't screw with mold if I were you. Not worth the effort. If it's already sporulating in solution, it's going to be very hard to clean.
If you want, PM me and I'll send you a wedge of my proven clean reishi culture.
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MorePies
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Registered: 02/09/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
#23781069 - 10/28/16 07:40 PM (7 years, 3 months ago) |
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Did you try making any other jars?
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: MorePies]
#23781085 - 10/28/16 07:49 PM (7 years, 3 months ago) |
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I just have that one jar of GL. I also have 3 of TV and one out of 3 HE that seem to have the same attributes of the contaminated GL.
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MorePies
Stranger
Registered: 02/09/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23781100 - 10/28/16 07:58 PM (7 years, 3 months ago) |
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So is that all contammed or one of the h. E.?
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: MorePies]
#23781189 - 10/28/16 08:35 PM (7 years, 3 months ago) |
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Yep, all of it is contammed.
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23781195 - 10/28/16 08:37 PM (7 years, 3 months ago) |
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The other 2 H.E. jars and all 3 of my L.E. jars appear to be doing ok so far.
Edited by Medhead (10/28/16 08:38 PM)
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23782261 - 10/29/16 10:25 AM (7 years, 3 months ago) |
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I put 3-4 ml of h202 in my apparently contaminated TV jars and within a couple of hours the millet flour I have in it and the rest of the growth(whatever it is) has begun to float to the top. Hmmm
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J W Fuengi
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Registered: 11/29/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
#23878150 - 11/29/16 12:29 PM (7 years, 2 months ago) |
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Hi, I also attended one of Peter's classes (in Oakland) and have since started jars of LC and millet grain spawn using the syringes he provided. About a week after seeing rapid growth in my GL LC and grain spawn jars blue-greens dot appeared on the sides of the jar and on some of the grains. None of my other 4 species (LE, HE, SRA, PS) showed signs of this type of growth. All jars were inoculated at the same time and under the same conditions - in the open air, on a clean table. So far I have not tried to treat the potential contamination.
How you had any luck treating the blue growth since your last post?
I haven't tried growing on agar yet, that will be my next move in the coming week. Have you had any success growing on agar and separating out the contamination?
I'm in NYC, if you're nearby maybe we could collaborate
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Medhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: J W Fuengi]
#23883133 - 11/30/16 09:44 PM (7 years, 1 month ago) |
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I'm in Alabama. Otherwise I'd love to meet up. I had a guy that attended the seminar in Nashville with me send me some of his GL from Peter. He used the honey LC. He also said that he keeps it in the fridge. Mine was maybe a week old and it started forming mycelium on top. We just had a baby so I haven't been agitated it in several days. I put it in the fridge to try to slow it down when we got home from the hospital.
Concerning my old contaminated jars, I dumped my contaminated jars of TV and GL on some wood chips in the ground outside just to see if it fruits one of these days.
I have already inoculated some grains with the new GL and It seems to be growing well. I'll know More when I get back home later this week.
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Quadman
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Registered: 04/23/16
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
#23883221 - 11/30/16 10:20 PM (7 years, 1 month ago) |
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"We just had a baby so I haven't been agitated "
Couldn't help it, that's funny. Congrats!
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Edited by Quadman (11/30/16 10:22 PM)
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Mrcloudy
Stranger than you.


Registered: 10/01/13
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Quadman]
#23883522 - 12/01/16 02:12 AM (7 years, 1 month ago) |
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Floating at the top is not indicative of a contamination, some species will even form mycelial mats on the surface of LC and Ganoderma can even fruit from this.

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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Speckles
Sober AF



Registered: 09/19/13
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Mrcloudy]
#23883613 - 12/01/16 04:03 AM (7 years, 1 month ago) |
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That's bonkers Mrcloudy
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Mrcloudy
Stranger than you.


Registered: 10/01/13
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Speckles]
#23883672 - 12/01/16 05:14 AM (7 years, 1 month ago) |
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I remember Roger Rabbit going on about how you can never fruit from LC, I am sure he was talking about psilocybin mushrooms and I had all these schemes to get something to fruit from LC in my mind but never got around to it. In the end it was just a forgotten jar sitting on a shelf for many months, when I found it again it looked like that. I also had something similar happen with lions mane but I can't seem to find any pictures of it.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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poofterFroth
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Mrcloudy]
#23883753 - 12/01/16 06:40 AM (7 years, 1 month ago) |
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Hydro reishi:
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: poofterFroth]
#23884746 - 12/01/16 02:51 PM (7 years, 1 month ago) |
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That is pretty cool about the LC And medhead, congrats on being a new father
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