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OfflineMedhead
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Ganoderma Lucidum LC contam. Or is it?
    #23767683 - 10/24/16 05:56 PM (7 years, 3 months ago)



I'm very new at this. So there is this white thin coating beginning to form in top of my LC. Any idea what it could be? Also, it sticks to the side of the jar if I turn it sideways.


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InvisibleMarty Mycfly
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23767707 - 10/24/16 06:03 PM (7 years, 3 months ago)

Hard to tell, but that is the big down side of LC, your going to have to put that on agar anyways to see if it's good or not.


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
    #23767810 - 10/24/16 06:33 PM (7 years, 3 months ago)

I was putting off agar work for now but, I may need to make that my next step. I've got to put a filter on my glovebox and get a fan and I should be ready to try some agar work.


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InvisibleMarty Mycfly
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23768262 - 10/24/16 08:58 PM (7 years, 3 months ago)

No, don't put a filter on a glove box. What you want to build is SAB (still air box), these work on the prinsible of "still air". It's just a tote turned upside down with two holes for your arms, don't be discouraged about doing agar work in one, I and many others have poured hundreds of dishes in SABS and they work great.
Where did you get the LC? If it was started in open air than it is more than likely contaminated.


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
    #23769040 - 10/25/16 06:02 AM (7 years, 3 months ago)

I got the LC syringes from Peter McCoy. He gave all of us some cultures as part of the radical mycology seminar that he hosts.


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InvisibleMarty Mycfly
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23770322 - 10/25/16 02:59 PM (7 years, 3 months ago)

Quote:

Medhead said:
I got the LC syringes from Peter McCoy. He gave all of us some cultures as part of the radical mycology seminar that he hosts.



Huh, well if he's a big deal you should probably have a good culture, however I find it weird that he opted to give everyone LC syringes instead of plates or slants, especially since the novice or even expert could tell if an LC is good or not with out testing it.
That could totally be good though, I have never made Reishi LC but I have heard that it makes a mat of mycelium on the top of the liquid, in fact I have read that there are companies in asia that culture reishi myc in huge vats of liquid culture and harvest the mycelium from the top since it has medical properties as well.


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OfflineGreg
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
    #23770480 - 10/25/16 03:51 PM (7 years, 3 months ago)

My reishi LC has never looked like that when I have made it before.
That stuff looks really gross.
Mine always looks clear-yellow with bits of myc floating in it like all other types of LC.
I do regularly agitate and mix my LCs when I make them though.

Unfortunately I don't have any on hand so I can't get photos.

Definitely test that on agar before you take it to grain or anything else.

Edit:
This is what red reishi looks like on agar by the way:


Edited by Greg (10/25/16 05:13 PM)


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
    #23770776 - 10/25/16 05:28 PM (7 years, 3 months ago)

He did say that he prefers liquid cultures but, for him there is a time and situation for both. He also talked about the advantages and disadvantages of both LC and agar. One of the biggest that I remember off hand is that one has a better chance to save some of the mycelium if there is some contamination but he does go into detail more in his book.

Regarding what you said specifically about Reishi LC, I haven't heard that mentioned before, I'm going look into it. Thanks for responding to my post.


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23770790 - 10/25/16 05:31 PM (7 years, 3 months ago)

The liquid has a yellow color. Is that what you are referring to or are you just talking about the white stuff on top?


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OfflineGreg
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23770806 - 10/25/16 05:36 PM (7 years, 3 months ago)

No, I was describing what my healthy reishi LC has looked like in the past.
Yours looks murky/cloudy, I'm not sure what that stuff is on top (doesn't look like healthy myc)
It doesn't look like any healthy LC I've ever seen.

Still, it's worth testing on agar.


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InvisibleMarty Mycfly
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
    #23770827 - 10/25/16 05:46 PM (7 years, 3 months ago)

I agree with him there is a time and place for LC, though I had bad experience when I was newer cultivator with it.
When I am in hurry to do large batches of grain I utilize Liquid inoculation by blending colonized agar dishes into sterile water and pour into bags, though I'm sure a healthy LC may have a faster leap off time than that.


--------------------
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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
    #23770932 - 10/25/16 06:20 PM (7 years, 3 months ago)

Thanks. Yeah I agree it does look murkey. The millet has turned a little darker as well.  I have some HE, TV, and LE and none of those look like the GL. I guess I'm about to get some plates and cook some agar up.


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InvisibleMarty Mycfly
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23771243 - 10/25/16 07:45 PM (7 years, 3 months ago)

Good luck dude:thumbup: Keep us posted and let us know how it goes.


--------------------
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Mycfly's Reishi    

                                                 


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Marty Mycfly]
    #23781021 - 10/28/16 07:20 PM (7 years, 3 months ago)

Dang it! It's mold! I tilted the jar over to pull out some LC and there are green specks stuck to the wall of the jar. Is it possible to inoculate some agar with it and allow it grow then, take a clean section of mycelium from the plate?


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OfflineGreg
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23781030 - 10/28/16 07:22 PM (7 years, 3 months ago)

It's possible but I wouldn't screw with mold if I were you. Not worth the effort.
If it's already sporulating in solution, it's going to be very hard to clean.

If you want, PM me and I'll send you a wedge of my proven clean reishi culture.


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OfflineMorePies
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Greg]
    #23781069 - 10/28/16 07:40 PM (7 years, 3 months ago)

Did you try making any other jars?


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: MorePies]
    #23781085 - 10/28/16 07:49 PM (7 years, 3 months ago)

I just have that one jar of GL. I also have 3 of TV and one out of 3 HE that seem to have the same attributes of the contaminated GL.


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OfflineMorePies
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23781100 - 10/28/16 07:58 PM (7 years, 3 months ago)

So is that all contammed or one of the h. E.?


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: MorePies]
    #23781189 - 10/28/16 08:35 PM (7 years, 3 months ago)

Yep, all of it is contammed.


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OfflineMedhead
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Re: Ganoderma Lucidum LC contam. Or is it? [Re: Medhead]
    #23781195 - 10/28/16 08:37 PM (7 years, 3 months ago)

The other 2 H.E. jars and all 3 of my L.E. jars appear to be doing ok so far.


Edited by Medhead (10/28/16 08:38 PM)


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