|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono
#23765984 - 10/24/16 05:38 AM (7 years, 3 months ago) |
|
|
Hey guys,
since I have asked a lot of questions in the Question and Answer Threads, I thought to start with a small log where I can show you my results so far and if there's a question, I can ask it directly here. Maybe some of the great growers here can help me along 
My Plan:Spore Print --> Agar --> Agar Transfers and Isolation until good ryzomorphic mycelium --> Agar wedge inoculation of rye jar --> Colonization --> Bulk --> Monotub.
First of all: I'm from Germany, my english is quite good, but sometimes I don't understand urban language, so don't be mad when I ask you to repeat some sentences in other words if I didn't understand them 
Ok, let's start.
Two monts ago I bought a Grow Kit, was my first one, before I never got in contact with psychedelic fungi. The growkit was just fine, gave me a great first flush of 10g cracker dried. The second flush was not that good, gave just a couple of very big shrooms but I took the chance to make a sporeprint.
SAB-Tek used: Spitball Jedi (https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771)
Pour Agar Tek: Bhodisatta (https://www.shroomery.org/forums/showflat.php/Number/21922023)
1. Sporeprint making (10.10.2016) I made a sporeprint with the help of bodhisatta in my SAB. Everything worked well so I had two good sporeprints. I skipped to make a spore syringe cause I directly started with agar.
2. Pour agar (15.10.2016) I made a LME-Agar and poured it into my petri dishes. Most plates were poured more than ten days ago and since now there is no contamination on them, so it seems, that I worked quite sterile while pouring the agar plates.
3. Inoculation of the agar plates (17.10.2016) I used sterile cotton swabs, wettened them a bit on the agar plate and then I got it on the spore print to grab some spores.
I've inoculated 4 agar plates.
2 with striking in a z-pattern and 2 with circle movement in the middle of the plate. I wrapped two with saran wrap and two with parafilm.
These are the results after one week (7 days) now:
BETTER PICTURES ARE SOME POSTES BELOW
Picture 1: Striking + Saran

Picture 2: Striking + Saran

Picture 3: Circle Movement + Parafilm

Picture 4: Circle Movement + Parafilm

After one week after inoculation they show as expected a good mycelium grow. Till now I can't see any contamination, but to be honest, I actually don't know whether I could recognize one if I see it.
So my first questions:
1. Can you see any contamination? Does everything look good? Is it fine until now? Too much or to less mycelium grow after a week? Is it normal that at the point where I put the spores onto there is dense white mycelium and at the edges its getting less dense and less white?
2. Is it a problem that there seem to be some spores not germinated (black dots in the mycelium)?
3. Do you think I should start with "cleaning"-transfer to other plates or just wait a bit? Shall I go with one plate, transfer to other agar and meanwhile just let it (the one i transferred from) colonize further to go for an isolation and rye inoculation?
Or shall I only start isolation after some cleaning-transfers?
4. If I want to transfer for cleaning, how big shall the wedge to cut out be? As small as possible? From the very edge of a myelium dot or better from the middle where it's dense and white?
Thanks in advanced for any help!
Regards.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/24/16 07:48 AM)
|
AgarStudent
Noob


Registered: 01/25/15
Posts: 550
Last seen: 2 years, 10 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766002 - 10/24/16 06:05 AM (7 years, 3 months ago) |
|
|
Hmm if you are serious about the hobby, I would recommend a laminar flow hood to avoid contaminations. You can build one yourself for not that much.
I don't know how you can avoid contamination without one to be honest, but some people seem to do without them? Your plates look okay, based on the resolution of the images you have provided.
I would wait until you have a bit more mycelial growth before you do any transfers. Maybe another few days to a week.
If you have good growth, somewhere on the edge would be best for a transfer. I think this is the fastest growing but of mycelium.
-------------------- Why reinvent the wheel when someone here has already done the science?
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766005 - 10/24/16 06:09 AM (7 years, 3 months ago) |
|
|
1. I can't really see much. The best way to take a pic of a plate is with a light shined at the side of the plate, they are bit blurry yes it's normal to be dense at the center and decrease outward.
2. You shouldn't be able to see black spores with the naked eye unless you literally didn't shake the syringe and a giant mass came out
3. You can transfer to more agar but I'd suggest taking a few transfers from a single individual plate think of a pyramid where the top is your original plate, everything below the "point" are transfers. You could let the original plate colonize if you don't need the plate and maybe it will pin. If it does transfer or "clone" the pin and then try to isolate. It's rare and highly unlikely that you will get an isolate directly from multispore and would take a shit ton of transfers to get one.
4. You transfer from a clean edge of the mycelium growth, with multispore your not going to see a lot if any sectoring so you really won't know what you're transferring. Your transfer wedge doesn't need to be any specific size right now, just make it big enough to get a good hold on the tip of your heat flamed then cooled scalpel.
-------------------- I would like to acquire anything I don’t have
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: PortabellaFella 1]
#23766027 - 10/24/16 06:28 AM (7 years, 3 months ago) |
|
|
I'm seeing what looks like some mold in all the plates (dead center, 7:30ish, and 1:00 in pic 1; 10:00 in pic 2; 2 spots just above dead center in pic 3; and 8 or 9 spots around center in pic 4), but like the other guys said, the pics aren't great. I also see what looks like clean cube mycelium. Get some better pics and the guys on here will be able to help a lot more. Plus that kind of growth after only 1 week is either some damn good spores, or isn't cube mycelium. Mine usually take longer than a week to even germinate, then I'm looking at another 2-3 days to get that kind of growth.
-------------------- Bug
Edited by thebug76 (10/24/16 06:40 AM)
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: PortabellaFella 1]
#23766043 - 10/24/16 06:37 AM (7 years, 3 months ago) |
|
|
Quote:
PortabellaFella 1 said:
2. You shouldn't be able to see black spores with the naked eye unless you literally didn't shake the syringe and a giant mass came out
He said he skipped the syringe and went cottonswab to print to agar. Thought I'd point that out, it could explain the spore clumps, but it kind of looks like some mold growth. I pointed it out in my previous reply, I totally missed you suggesting OP didn't shake the syringe or I would be pointed that out then also.
-------------------- Bug
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766056 - 10/24/16 06:49 AM (7 years, 3 months ago) |
|
|
Thank you very much for your answers.
Here are some better Pictures I hope:
Picture 1:
Picture 2:

Picture 3:

Picture 4:

I hope they are better now
To your postings:
Quote:
Hmm if you are serious about the hobby, I would recommend a laminar flow hood to avoid contaminations. You can build one yourself for not that much.
Maybe in some weeks I will build one, but at the moment it must work with "only" a SAB.
Quote:
2. You shouldn't be able to see black spores with the naked eye unless you literally didn't shake the syringe and a giant mass came out
I made it with a spore print and a cotton swab, the cotton swab was black when touching the agar plate, so thats the reason for the black dots. Some germinated and disappeared but some stay.
Quote:
It's rare and highly unlikely that you will get an isolate directly from multispore and would take a shit ton of transfers to get one.
So the first step should be cleaning-transfer and isolation is something I can think about after first grow and cloning?
Quote:
I'm seeing what looks like some mold in all the plates. Plus that kind of growth after only 1 week is either some damn good spores, or isn't cube mycelium. Mine usually take longer than a week to even germinate, then I'm looking at another 2-3 days to get that kind of growth.
Really? Also on the new pictures? Damn
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766064 - 10/24/16 06:59 AM (7 years, 3 months ago) |
|
|
It's early, I skipped over the cotton swab inoculation my bad. That's myc. It's gonna be a little difficult to transfer from a leading edge since there are so many colonies with the spores swabbed so thick. Yes, clean, transfer, grow , clone then isolate. The new pics are much better.
-------------------- I would like to acquire anything I don’t have
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: PortabellaFella 1]
#23766081 - 10/24/16 07:12 AM (7 years, 3 months ago) |
|
|
The new pics look better. I think some of the spots I was seeing is either clumps or dark spots on the lids of your plates. On the new pics there's a couple of spots in pic 2 that are either heavy spore clumps or are about to turn green. But even if it does, you still have good stuff to work from.
-------------------- Bug
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766127 - 10/24/16 07:52 AM (7 years, 3 months ago) |
|
|
Quote:
PortabellaFella 1 said: It's early, I skipped over the cotton swab inoculation my bad. That's myc. It's gonna be a little difficult to transfer from a leading edge since there are so many colonies with the spores swabbed so thick. Yes, clean, transfer, grow , clone then isolate. The new pics are much better.
hehe I just forgot that it's early in the morning in the US, here it's 4 pm
But the plate on picture 4 - can I transfer from the leading edge there?
How shall I transfer from the other pleates? Directly a wedge with a whole "myelium dot"?
Shall I transfer as soon as possible or wait a bit? Which points do you recommend for transfer?
Quote:
thebug76 said: The new pics look better. I think some of the spots I was seeing is either clumps or dark spots on the lids of your plates. On the new pics there's a couple of spots in pic 2 that are either heavy spore clumps or are about to turn green. But even if it does, you still have good stuff to work from.
Thank you very much, I was really afraid that it's mold, cause with my eyes I can't see anything strange.
Looking at plate of picture two I cant see anything turnin green. Maybe it's the picture, but I will observe it.
"To work with" means cleaning-tranfers, right? Shall I start asap or wait a bit?
Which points do you recommend to transfer?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766143 - 10/24/16 08:03 AM (7 years, 3 months ago) |
|
|
Picture 4 I would cut a wedge from the top colony about 1 o'clock. Just take a wedge from the leading edge inward toward the center but away from the fluffy white mass. You could do it now or wait to see if any more aggressive myc forms.
-------------------- I would like to acquire anything I don’t have
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: PortabellaFella 1]
#23766157 - 10/24/16 08:15 AM (7 years, 3 months ago) |
|
|
Right around the 12:00 position in pic 2 looks really thick and kind of dark, that's where I said possibly mold. Could be lighting tho. But in any case you have good rhizo growth near the bottom and just above center. There's several places you could get good transfers from, just get from the leading edge. I don't even really do wedges when I'm making plate to plate transfers, only a really small piece about the size of a grain of rice. Less is more when going agar->agar.
I would go ahead and do it. If you wait much longer, that plate is going to be all grown together and overrun.
-------------------- Bug
Edited by thebug76 (10/24/16 08:17 AM)
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766319 - 10/24/16 09:57 AM (7 years, 3 months ago) |
|
|
Thank you for your answers.
Shall I also transfer from plate on picture 4? The mycelium is not that big on that but at least some kind of rhizomorphic
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766382 - 10/24/16 10:35 AM (7 years, 3 months ago) |
|
|
I would go with portabellafella1's advice on pic 4. I've read a lot of his stuff on here and honestly, he's probably more qualified than I am.
-------------------- Bug
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766460 - 10/24/16 11:12 AM (7 years, 3 months ago) |
|
|
Quote:
thebug76 said: I would go with portabellafella1's advice on pic 4. I've read a lot of his stuff on here and honestly, he's probably more qualified than I am.
Ok, fine, and what do you think of the other plates, also transfer?
I will update a picture where I mark the transfer points right now
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766488 - 10/24/16 11:23 AM (7 years, 3 months ago) |
|
|
Anywhere you have clean looking rhizomorphic growth, you can transfer from. I wouldn't get too transfer happy tho until I had a few plates gaurenteed clean. And even then, it doesn't take much. It takes a bunch of plates when you're isolating. Plates grow fairly quick once you get them going, so it's not like tou need buttloads sitting around. Making sure you have a clean culture can generally be done in just a few transfer.
-------------------- Bug
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766493 - 10/24/16 11:27 AM (7 years, 3 months ago) |
|
|
All of those plates look good to me. I feel like taking transfers from more than one plate is redundant at this stage because it's all random as hell MS anyway. Just focus on getting clean healthy growth.
Generally what I do is I make 2 germination plates, take 2-4 transfers from the best looking one, take 2-4 transfers from the best looking one of those, etc. I always keep the old plates until I am certain I like the new transfers so I can go back a step if needed.
Don't worry if you can't get rhizo growth at first (or at all), tomentose growth can be healthy too. My burma took ~5 transfers to show rhizo traits and it still reverts back to tomentose when I transfer to new plates sometimes. On the other hand, my KSSS showed rhizo growth on the first transfer and my APE showed it on the germination plate. Point is, it doesn't necessarily matter if it's rhizo, it only matters if it's healthy.
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: Greg]
#23766503 - 10/24/16 11:33 AM (7 years, 3 months ago) |
|
|
-------------------- I would like to acquire anything I don’t have
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: Greg]
#23766529 - 10/24/16 11:43 AM (7 years, 3 months ago) |
|
|
Quote:
Greg said: All of those plates look good to me. I feel like taking transfers from more than one plate is redundant at this stage because it's all random as hell MS anyway. Just focus on getting clean healthy growth.
Generally what I do is I make 2 germination plates, take 2-4 transfers from the best looking one, take 2-4 transfers from the best looking one of those, etc. I always keep the old plates until I am certain I like the new transfers so I can go back a step if needed.
Don't worry if you can't get rhizo growth at first (or at all), tomentose growth can be healthy too.
Thank you for your answer (and of course thanks to all the others).
So you suggest to only transfer from one dish is better than from all the dishes, right? Which one would you chose? Also plate on Picture 4?
And you cut more than one wedge of ONE plate and transfer to different new plates, did I understand correctly? So you make e.g. 3 plates out of one? Isn't it a contamination risk if I open the plate that long to take for example 3 wedges out of it?
Here I have a picture of plate 4. Which one of the triangles would you choose for transferring? Just to get an idea how near to the "center" of the growth I can go.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766542 - 10/24/16 11:49 AM (7 years, 3 months ago) |
|
|
All three look clean. Me personally, I would go with red. Either would be sufficient, but newer growth tends to be more vigorous. Then I would get one from around the bottom and from the upper left as well.
-------------------- Bug
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 25 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AgarStudent]
#23766550 - 10/24/16 11:51 AM (7 years, 3 months ago) |
|
|
Quote:
AlCapone2k said: Two monts ago I bought a Grow Kit, was my first one, before I never got in contact with psychedelic fungi. The growkit was just fine, gave me a great first flush of 10g cracker dried. The second flush was not that good, gave just a couple of very big shrooms but I took the chance to make a sporeprint.
I would have taken a clone of that growkit. It is a known fruiting strain which was presumably carefully selected.
Seems you were streaking the plates and having growth in many places. I would only dip the swab in the very centre, this way if any spots other than the centre show growth it would be unwanted growth and you know your SAB technique is not great, or that your plates were not sterile to start with. It also means it will grow outwards from the centre so its is easier to select a clean leading edge. Having many dots of growth means they will grow into each other. Instead of dipping swabs I have picked a little bit off with a sterile tweezers and placed it in the middle of a plate, I did this as my plates were quite moist and I was worried about it absorbing nutrients, though others say they have no issue.
Quote:
AgarStudent said: Hmm if you are serious about the hobby, I would recommend a laminar flow hood to avoid contaminations. You can build one yourself for not that much.
I don't know how you can avoid contamination without one to be honest, but some people seem to do without them?
Surely you have heard of SABs? I have been simply using a long clear plastic bag lately, which is sprayed with water..
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766568 - 10/24/16 11:58 AM (7 years, 3 months ago) |
|
|
Quote:
thebug76 said: All three look clean. Me personally, I would go with red. Either would be sufficient, but newer growth tends to be more vigorous. Then I would get one from around the bottom and from the upper left as well.
What do you think of that?

So I take three wedges out of one plate and transfer to three new plates. So I open the dish, cut a wedge, close the first dish, open the second, put it in, open the old dish again, cut again and so one.
Do I have to flame sterilize or take a new sterilized "one use"-Skalpel every cut or can I use one scalpel for all the three transfers as long as it touches only agar?
Quote:
blackout said: I would have taken a clone of that growkit. It is a known fruiting strain which was presumably carefully selected.
Seems you were streaking the plates and having growth in many places. I would only dip the swab in the very centre, this way if any spots other than the centre show growth it would be unwanted growth and you know your SAB technique is not great, or that your plates were not sterile to start with. It also means it will grow outwards from the centre so its is easier to select a clean leading edge. Having many dots of growth means they will grow into each other. Instead of dipping swabs I have picked a little bit off with a sterile tweezers and placed it in the middle of a plate, I did this as my plates were quite moist and I was worried about it absorbing nutrients, though others say they have no issue.
Yes, I was streaking the plates, actually it was a suggestion from bodhisatta. Since in his agar tek he talks about centre inoculation I did it with some plates to. In future I will only inoculate to the center.
And to the clone thing: Yeah, actually before harvesting my first flush I thought I won't go into this hobby, so I didn't care. Then I totally regret that and as the second flush came, I asked in some thread here and they told me that cloning a 2nd-flush mushroom isn't that good, so I made sporeprints.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/24/16 12:03 PM)
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766576 - 10/24/16 12:02 PM (7 years, 3 months ago) |
|
|
Correct. And I would flame after every transfer, just to be safe. Hell I flame after every hole in my pf jars. I've had a lot of contam troubles tho, so I'm extra cautious.
-------------------- Bug
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766584 - 10/24/16 12:05 PM (7 years, 3 months ago) |
|
|
Be sure to cool your scalpel in an uncolonized portion of the agar before cutting or you'll kill your myc
-------------------- I would like to acquire anything I don’t have
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766586 - 10/24/16 12:06 PM (7 years, 3 months ago) |
|
|
Quote:
PortabellaFella 1 said: Be sure to cool your scalpel in an uncolonized portion of the agar before cutting or you'll kill your myc
yes, of course, thank you I read the thread of stro about cleaning more than one time
But I ask myself about the best procedure: Skalpel in the SAB - take it out - flame it red hot - get it back to SAB and so on? Shall I wait a bit after getting my hand with the scalpel back to the SAB to let everything settle?
Quote:
thebug76 said: Correct. And I would flame after every transfer, just to be safe. Hell I flame after every hole in my pf jars. I've had a lot of contam troubles tho, so I'm extra cautious.
Ok, fine. So I'll go for this plate now.
Unforunately I only have "one use"-Skalpels, they can not be flamed that good because they have plastic around the metal. But it's no problem, they cost 40 euro-cents so I can afford three of them
I will buy a multi-use skalpel next time
And what shall I do with the other plates? Also transfer or better wait and see what happens to them? Maybe they are conti free so I can directy use them for rye? Suggestions?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/24/16 12:08 PM)
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766595 - 10/24/16 12:08 PM (7 years, 3 months ago) |
|
|
If they're different strains, I would make tranfers from them too. Otherwise, I would just hold on to them til you know you're happy with your next set.
Yea, if you're sure they're clean, transfer some wedges to grain jars. You could probably use the entire piece of agar, but I read somewhere (can't remember where right at the moment) that contams like to sneak in around the edges. So, I generally cut all the way around the inside about a quarter of an inch or so from the side, then cut that circle into wedges and use that, leaving outer edge of agar alone.
-------------------- Bug
Edited by thebug76 (10/24/16 12:14 PM)
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766607 - 10/24/16 12:13 PM (7 years, 3 months ago) |
|
|
No, they are made from the same sporeprint.
Ok, I let them rest to see what will happen.
If mycelium colonizes the whole agar, it will start to fruit. Can I then still take a wedge to colonize new ones or must it be done at least before it's going to fruit?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766618 - 10/24/16 12:17 PM (7 years, 3 months ago) |
|
|
I edited my last post to answer the question about rye, but not before you saw it I guess.
If it pins inside the plate, clone it. And yes you can still transfer with it.
-------------------- Bug
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766628 - 10/24/16 12:18 PM (7 years, 3 months ago) |
|
|
You already pretty smart on this stuff OP. It won't be long, I'll be asking you questions.
-------------------- Bug
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
#23766639 - 10/24/16 12:23 PM (7 years, 3 months ago) |
|
|
Quote:
thebug76 said: You already pretty smart on this stuff OP. It won't be long, I'll be asking you questions.
Oh, thank you very much Before I even bought one petri dish, I read almost all tutorials here, just to be sure to know everything so that I don't mess up. I'm still a student and have not that much money to start things over again and again because they fail
So I'm really happy that my first agar inoculation seem to be done good at the moment
Buddy, I like your small trays in your signature. I think this could be also a possibility for me, since I only have a small students appartment and not always time, place and effort to make a big monotub. Is this perculit on the bottom?
Quote:
thebug76 said: Yea, if you're sure they're clean, transfer some wedges to grain jars. You could probably use the entire piece of agar, but I read somewhere (can't remember where right at the moment) that contams like to sneak in around the edges. So, I generally cut all the way around the inside about a quarter of an inch or so from the side, then cut that circle into wedges and use that, leaving outer edge of agar alone.
Ok, fine, I will do the transfer of plate nr. 4 in a couple of minutes, all the three transfers showed in the second transfer picture.
Thank for your help!
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/24/16 12:24 PM)
|
thebug76
2 years in.



Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23766644 - 10/24/16 12:27 PM (7 years, 3 months ago) |
|
|
No problem. Good luck.
-------------------- Bug
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23767083 - 10/24/16 02:52 PM (7 years, 3 months ago) |
|
|
Ok, the transfering process was a big mess
My SAB was so soapy and dark (it's evening here now) that I wasn't able to see well into my dishes. After opening them I cut out a wedge but wasn't able to pic it up.
Are you picking it up by stabing into it (like with a sword) or do you use the scalpel like a spoon?
At the end my plate nr. 4 is now totally disturbed and because I hade to lift it up with my hands to see the wedge, it will have contamns for sure
But anyway, next time I'll do better and prepare better.
Unfortunaetely I also saw, that there werde mold and bacteria in some of my NOT INOCULATED poured agar dishes. Don't know what has happened there but it seems the saran wrap didn't seal that well or something, because the dishes of the same day parafilmed are still good.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AgarStudent]
#23767112 - 10/24/16 03:01 PM (7 years, 3 months ago) |
|
|
Quote:
AgarStudent said: Hmm if you are serious about the hobby, I would recommend a laminar flow hood to avoid contaminations. You can build one yourself for not that much.
I don't know how you can avoid contamination without one to be honest, but some people seem to do without them?
you absolutely do not need a flowhood for this hobby, a Still Air Box (SAB) is far more than sufficient for anyone that's not making 100s of jars of spawn a week
my whole pour agar tek is brought to you by a small 66q SAB.
https://www.shroomery.org/forums/usergallery.php/gallery/300678/imgpl/5/imgpp/all/folder/agar that's my agar gallery you can see how I started off pretty nooby and things got better, all the agar there was done in a SAB. and it's all poured plates.
you should be able to get an idea of the progression of how things look as you take transfers
Do not worry about getting an isolate. just get some clean healthy looking growth and go with it.
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
#23767183 - 10/24/16 03:21 PM (7 years, 3 months ago) |
|
|
yeah, I watched your gallery, so far in agar work as you I'd really like to be. As you can read, I totally messed my first transfer 
Now I really hope that at least either one transfer petri will do it's job or at least the source petri is not that damaged that I can throw it away.
Next time I really need a light source next to my SAB, otherwise it won't work.
Bodhi, are you picking transfer wedges up by stabing into it (like with a sword) or do you use the scalpel like a spoon?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23767189 - 10/24/16 03:23 PM (7 years, 3 months ago) |
|
|
let me know if you can't view these videos in germany
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
#23767490 - 10/24/16 04:56 PM (7 years, 3 months ago) |
|
|
I can watch them, thank you. Answer was given, stabbing the wedge is ok, so I'll do that next time. Unfortunately I tried to pull out the wedge with the scalpel used like a spoon, almost impossible without messing around in the agar.
To the first Video: He is also lifting up the dishes and holding them towards his body to see the mycelium/place to cut well. So I also can do that with an open petri dish? The Problem is my angle - when sittin in front of the SAB I have a very bad angle to the petri dishes lying on the towel. Better would be an angle from above the petri. To be able to see better I just lifted the petri dish as he does with the pasty plate.
Is it sterile enough or will the lifting cause contamns to get into the plate?
To the second video: Do you also drop the whole agar plate into the rye or do you cut a wedge out and put it in your jar?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/24/16 04:57 PM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23767529 - 10/24/16 05:07 PM (7 years, 3 months ago) |
|
|
you can cut big wedges
 you can practice with agar first so you don't fuck up when you get to using the plates that have precious mycelium on them
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23767566 - 10/24/16 05:19 PM (7 years, 3 months ago) |
|
|
yeah, I think I should do that. It's ok, it was my first time transferring and I fucked up, next time will be better :P
I have to do a transfer of a oyster mushroom these days anyway, so I can practice it. If I mess up, it's no problem cause I can get into the shop and buy a new mycelium syringe for oysters, of course not for cubensis So it hurts more if I mess up with them.
Now it's waiting time.
The wedges put onto the new plate should colonize the agar with mycelium within 7 days, right?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23767587 - 10/24/16 05:25 PM (7 years, 3 months ago) |
|
|
how many days depends on a lot of things, genetics, temperature, size of the petri dish, what kind of agar
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
#23767605 - 10/24/16 05:31 PM (7 years, 3 months ago) |
|
|
Ok, thank you very much for all your help! You really helped me a lot.
Now I wait until my new plates colonize from the wedge. I'll update with pictures.
Have a nice day all of you
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
fIsh in my head
fadedstar

Registered: 01/25/03
Posts: 1,150
Loc: 4500 ASL
Last seen: 6 years, 13 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23768540 - 10/24/16 10:34 PM (7 years, 3 months ago) |
|
|
I'm back in the hobby myself and trying agar for the first time. I going for the long run since i'm trying to wake some spores printed in 2003 !
I trully felt like a noob trying to scrape like a clumsy mofo a very dry print. Seems to me that I took way too much time during the whole process.
I'm following your thread because need to learn transfer form MS agar. You are a week ahead of me but for some reason I feel all my shit will contam 
good luck!
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: fIsh in my head]
#23772441 - 10/26/16 06:56 AM (7 years, 3 months ago) |
|
|
Hey guys,
I have a question about sterile work with an SAB and a propane/butane torch to sterilize the scalpel.
How do you do that?
Do you let your propane torch running outside the SAB, make a transfer, then you take out the scalpel of the SAB with one hand towards the torch, flame it and go back with the scalpel into the SAB? Don't you have to sanitize your gloves with iso afterwards?
Or is it better this way: Take one hand out of the SAB to grab the torch. In the other Hand, still inside the SAB, you have the scalpel and let it go out a bit of the free hole of your SAB to flame it with the torch? I think this would be better, because the scalpel doesn't leave of the SAB so much. But isn't the air swirled up by the torch too much in front of the hole of SAB in this method?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772503 - 10/26/16 07:43 AM (7 years, 3 months ago) |
|
|
I have my tourch running on low beside the work area, I do pull the scalpel out from the work area to flame it. It sits just beside the flowhood.
I try not to rush really hard and stir the air, movements are careful.
You can re-wet your gloves every so often but I would not be worried about rewetting them each time you remove them.
Still air or sterile procedure is the primary focus. If you're wanting to double check your scalpels sterility wrap it in foil and pressure cook it before your session, that way every crevasse is sterile.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23772526 - 10/26/16 07:59 AM (7 years, 3 months ago) |
|
|
Ok, fine, so it's no problem, that my hand is leaving the SAB for a couple of seconds. Thought it could be a problem.
are 10 minutes PC for the scalpel enough?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772534 - 10/26/16 08:06 AM (7 years, 3 months ago) |
|
|
Call it 15 minutes. If your planning on growing a lot a flow hood helps a lot.
66qts arent as nice to work in as a 2x2 or 2x4 flowhood. No large scale commercial growers use sabs in their laboratories over flowhoods. The cost is a few hundred $.
Sweet log so far
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772567 - 10/26/16 08:19 AM (7 years, 3 months ago) |
|
|
Actually my plan is just to get some mushrooms for self-usage As long as I can harvest some grams per month or twice a year a lot for storage, I'm happy
A flowhood is a bit expensive for me, eventhough it could be really interesting. Sometimes one can find whole sterile work banks like in laboratories on ebay for some bucks. But actually, eventhough I'd find one, my students appartment is too small for it
Thank you very much.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772585 - 10/26/16 08:24 AM (7 years, 3 months ago) |
|
|
Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23782892 - 10/29/16 02:49 PM (7 years, 3 months ago) |
|
|
Quote:
filthyknees said: Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
Wow dude. That's pretty fucking lame.
|
morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur]
#23782909 - 10/29/16 03:00 PM (7 years, 3 months ago) |
|
|
Quote:
azur said:
Quote:
filthyknees said: Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
Wow dude. That's pretty fucking lame.

Damn Azur... lol
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur] 1
#23782910 - 10/29/16 03:00 PM (7 years, 3 months ago) |
|
|
It's just getting your tax money back. Niggers do it, why not
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
#23782944 - 10/29/16 03:17 PM (7 years, 3 months ago) |
|
|
Well I don't think it's cool. Filthy and I have hung out many, many times. He's a great guy and he's more than capable. I am happy to help anyone in need, but that's the key word-need
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur]
#23783046 - 10/29/16 03:56 PM (7 years, 3 months ago) |
|
|
Hey guys,
yesterday I started my first Agar to grain. Furthermore I inoculated two grain jars with a mycelium syringe of an oyster mushroom bought from a laboratory for mushroom cultivation, they supply big growing companies so I think it's 100% sterile
Here is one rye jar for example, one which I inoculated with the mycelium syringe.


But I have some questions:
1. Is it normal, that there are so many water drops on the glas? Is my humidity to high in the jars? I let the rye dry for one hour after boiling/simmering and shaked it many times during drying. Then I PCd the jars.
2. On the picutre I marked one rye berry with a red circle. The rye berry sticks to the glas a bit under the lid, far away from the rest of the rye berries (can't be seen well on the picture). It seems that there is some white stuff on it, could be mold, because it's a bit hairy. Better to toss the jar away now or shall I wait some days to see what will happen?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23783055 - 10/29/16 03:58 PM (7 years, 3 months ago) |
|
|
1. How long did you boil it? I have this issue with grain and spawn bags sometimes.
2. Could be oyster mycelium. Wait a few more days to be sure. Did you shake the jar after agar inoculation? If it is so, it starts to colonize on different locations inside the jar.
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23783071 - 10/29/16 04:04 PM (7 years, 3 months ago) |
|
|
I soaked them in water for about 18 hours, then I put the pot directly on the stove without changing the water they were soaked in.
I let the water boil and then reduced the heat so that it simmered, I let it simmer for about 10 Minutes, not more. As I saw that some berries are popping, I directly took the pot from the stove.
Then I put the rye into a screen, let it there for about an hour and shaked it every 5 minutes to get all the water out.
After that I put some berries on a paper towel and there was no water after removing them. So they seem to be perfect, therefore I put them in the jars and PCd them for 2 hours (not only 90 minutes, because german PC aren't able to reach 15psi).
Shouldn't there be any water on the glas?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23783090 - 10/29/16 04:10 PM (7 years, 3 months ago) |
|
|
Sounds good. I like to spread the hot grain over a big surface. This way the grain dries very fast. Some moisture is absorbed by the grain after some days. It's the same when you inoculate with liquid culture. 1-2cc makes the grain look too wet but it gets absorbed after some time.
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23783124 - 10/29/16 04:27 PM (7 years, 3 months ago) |
|
|
Ok fine, so I'll wait some days
Actually I didn't see any "problem", just wanted to update my nooblog
I put my jars in a clear tote (same like my SAB), it's in a room, that has about 20°C (68 F) and is quite still (considering air movement). I hope that's fine 
EDIT: After inoculation with the agar wedge, I knot that I should not shake, just take care, that the agar wedge is covered by grains - did that!  But what shall I do with the jars inoculated with the MS Syringe? Unfortunately my SAB is not that high and the syringe is very big, so I wasn't able to shoot directy at the edge of the jar, so I shooted on the grain from above it (openin the lid just a crack). Shall I shake the grain jar diectly or let the myeclium from the syringe recover some days?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/29/16 06:28 PM)
|
filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23788528 - 10/31/16 12:55 PM (7 years, 2 months ago) |
|
|
Yeah dude pretty lame I like to remember where I started, wasn't anything special living in the ghetto poor trying to stay sane
No super legit set up but that rhy bag got me off the food stamps amen. Don't mind having a good laugh at myself not that I've gone too far
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23788693 - 10/31/16 02:02 PM (7 years, 2 months ago) |
|
|
haha, great man 
To make a small update:
My agar plates are growing and growing, made some transfers and I'm awaiting a totally clean plate to make some tiger drops. Maybe in a week or so I'll be ready for that
But I have noticed on two of my first agar plates some "water drops" on the mycelium. Not on the lid of the petri dish, directly on the mycelium. Especially there, where the mycelium seem to be dense.
It appeared on these agar plates, that I inoculated 14 days ago and that seem very clean, but I did not take any transfer from them, because I choose other plates to do that. So I let these plates "grow out". They really look like very small waterdrops, but if I shake the plate a bit, they don't move or so. They realy don't look like bacteria oder mold, just like small drops.
Is it a problem? Contamn? Bad conditions for the mycelium?
Here's a picture.

Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/31/16 02:03 PM)
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23800113 - 11/04/16 09:01 AM (7 years, 2 months ago) |
|
|
Hey guys,
a small update.
I now have 6 half quart jars (quart jars don't fit into my PC) full of rye - all sterilized.
My agar work is also going on, I was able to "produce" two clean agar cultures after three transfers of B+.
Now they seem really clean and show a nice rizomorphic growth.
Is it possible to transfer wedges from these two plates to grain jars now or shall I better wait some days more until I inoculate jars with them? If it's ok, how many jars can I inoculate with one plate?
And if I better wait, is it ok to store sterilized rye jars without inoculating for some days or do they have to be inoculated immediately after sterilization?

Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23800317 - 11/04/16 10:57 AM (7 years, 2 months ago) |
|
|
Storing sterilized jars for some days is OK.
You can inoculate with a colonized piece of your agar dish right now. But I often wait some more time. I like to use bigger pieces of agar.

I prefer to use at least the size of the marked '1/4'. The most time I use about the half plate for a 'quart' jar (a little bigger than a quart -> 1100ml jar).
You get more inoculation points with bigger pieces of agar.
Edited by enlightenment (11/04/16 11:04 AM)
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23800734 - 11/04/16 01:54 PM (7 years, 2 months ago) |
|
|
Thank you very much. If it's ok to wait some days before inoculation, then I better wait until more of the plate is colonized by the mycelium
Do you think the plates are clean enough or shall I better make another transfer?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23800743 - 11/04/16 01:59 PM (7 years, 2 months ago) |
|
|
They look fine
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23825205 - 11/12/16 08:29 AM (7 years, 2 months ago) |
|
|
A small update on my Noob Log
Here for example two of my 6 jars colonizing by a cubensis agar wedge
Look good, hm?
I think I'll give the left one a shake today, or better wait? The right one will get a shake tomorrow or the day after.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23850999 - 11/20/16 10:10 AM (7 years, 2 months ago) |
|
|
Again a small update.
Here the two jars after shaking.
Inoculation: 4th Nov 1st shake: 13th Nov 2nd shake: 18th Nov.

I gave them a third shake couple of minutes ago, will now wait until they recolonize (I hope in about 2 days) and then spawn them to CVG in a tray. Wanted to start with the monotub directly but unfortunately these two jars were inoculated a week before the others so I will spawn them separately from the others in small trays and then fruiting them in filterbags. I'll give it a try
Regards.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23874186 - 11/28/16 04:44 AM (7 years, 2 months ago) |
|
|
Today I'm going to start my first mini mono.
I have a 20qt mini tote. I drilled one 1,25" hole on every side, at sub level on the long side and unter the lid on the short sides.

I am going to spawn the jars in a ratio 1:2 to CVG prepared with the bucket tek.
Here are 5 of the jars (in total they are 8, but all look the same.

The two ont he left with the red-white lid are concerning me, they are not fully colonized, there are some grains that aren't colonized yet, maybe it's bacteria.
I am thinking of spawning the good jars to the mini mono and these two jars on the left to a small tray that I will put to my SGFC.
What do you think guys?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 4 hours, 22 seconds
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23874281 - 11/28/16 06:33 AM (7 years, 2 months ago) |
|
|
I don't know about bacteria, but I'd like to ask where you found proper mason jars in Germany? Or did you import them from eBay? I use plastic PP5s from the smart shop but it would be nice to have some proper mason jars, but the cheapest I saw was from 5-10 euro per jar.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: Tiamo]
#23874291 - 11/28/16 06:39 AM (7 years, 2 months ago) |
|
|
Hey mate,
theese aren't mason jars, they just look like they were. They also don't have a two-piece lid, only one piece.
They are very nice though, because the lid is very good and the seal very well.
Just searh for "Quattro Staggioni", you get them on amazon for about 2,80 Euro per one or go and buy them at "Butlers" or "Karstadt". I found them also on Marktkauf for only 2,30 Euro, I directly bought six.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
Tiamo
Trust in LITFA




Registered: 04/07/16
Posts: 1,935
Loc: Amsterdam
Last seen: 4 hours, 22 seconds
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23874365 - 11/28/16 07:26 AM (7 years, 2 months ago) |
|
|
Yea, I've seen those Quattro Staggioni jars around in the shops. They're pretty good. Too bad, I really want some mason jars with two piece lid.
--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: Tiamo]
#23874428 - 11/28/16 07:57 AM (7 years, 2 months ago) |
|
|
I don't see any advantage of the two-pieces lids. In Germany they are very expensive. Get these Quattro Staggioni glasses, they served me very well.
There is only one german company producing two-pieces lids that I know, it's Leifheit. But I tested these and it's a mess to open and close them with the ring-part of the lid. It was a total mess in my SAB to open them.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
|
Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23877147 - 11/29/16 03:09 AM (7 years, 2 months ago) |
|
|
One week ago I did three small trays for my SGFC, because I hade some jars that needed to be spawned but not enough for a mini mono. Two days ago I gave them into fruiting conditions in my SGFC.

Yesterday evenining I did my first two mini monos. 20l with about 7qt of Spawn and Sub in a 1:2 ratio with CVG (just bucket tek, no pasteurizing or sterilizing).


I will update when they are colonizing and of course when moving into fruiting 
Regards.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
|
|