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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: fIsh in my head]
#23772441 - 10/26/16 06:56 AM (7 years, 3 months ago) |
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Hey guys,
I have a question about sterile work with an SAB and a propane/butane torch to sterilize the scalpel.
How do you do that?
Do you let your propane torch running outside the SAB, make a transfer, then you take out the scalpel of the SAB with one hand towards the torch, flame it and go back with the scalpel into the SAB? Don't you have to sanitize your gloves with iso afterwards?
Or is it better this way: Take one hand out of the SAB to grab the torch. In the other Hand, still inside the SAB, you have the scalpel and let it go out a bit of the free hole of your SAB to flame it with the torch? I think this would be better, because the scalpel doesn't leave of the SAB so much. But isn't the air swirled up by the torch too much in front of the hole of SAB in this method?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772503 - 10/26/16 07:43 AM (7 years, 3 months ago) |
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I have my tourch running on low beside the work area, I do pull the scalpel out from the work area to flame it. It sits just beside the flowhood.
I try not to rush really hard and stir the air, movements are careful.
You can re-wet your gloves every so often but I would not be worried about rewetting them each time you remove them.
Still air or sterile procedure is the primary focus. If you're wanting to double check your scalpels sterility wrap it in foil and pressure cook it before your session, that way every crevasse is sterile.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23772526 - 10/26/16 07:59 AM (7 years, 3 months ago) |
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Ok, fine, so it's no problem, that my hand is leaving the SAB for a couple of seconds. Thought it could be a problem.
are 10 minutes PC for the scalpel enough?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772534 - 10/26/16 08:06 AM (7 years, 3 months ago) |
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Call it 15 minutes. If your planning on growing a lot a flow hood helps a lot.
66qts arent as nice to work in as a 2x2 or 2x4 flowhood. No large scale commercial growers use sabs in their laboratories over flowhoods. The cost is a few hundred $.
Sweet log so far
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772567 - 10/26/16 08:19 AM (7 years, 3 months ago) |
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Actually my plan is just to get some mushrooms for self-usage As long as I can harvest some grams per month or twice a year a lot for storage, I'm happy
A flowhood is a bit expensive for me, eventhough it could be really interesting. Sometimes one can find whole sterile work banks like in laboratories on ebay for some bucks. But actually, eventhough I'd find one, my students appartment is too small for it
Thank you very much.
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23772585 - 10/26/16 08:24 AM (7 years, 3 months ago) |
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Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23782892 - 10/29/16 02:49 PM (7 years, 3 months ago) |
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Quote:
filthyknees said: Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
Wow dude. That's pretty fucking lame.
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morty422
Wuba-luba-dub-dub!


Registered: 07/06/16
Posts: 988
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur]
#23782909 - 10/29/16 03:00 PM (7 years, 3 months ago) |
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Quote:
azur said:
Quote:
filthyknees said: Nice. I grew my first half # living in the living room of a one bedroom using food stamps to get rhy grain.
So... You can do eeet!
Wow dude. That's pretty fucking lame.

Damn Azur... lol
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur] 1
#23782910 - 10/29/16 03:00 PM (7 years, 3 months ago) |
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It's just getting your tax money back. Niggers do it, why not
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
#23782944 - 10/29/16 03:17 PM (7 years, 3 months ago) |
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Well I don't think it's cool. Filthy and I have hung out many, many times. He's a great guy and he's more than capable. I am happy to help anyone in need, but that's the key word-need
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: azur]
#23783046 - 10/29/16 03:56 PM (7 years, 3 months ago) |
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Hey guys,
yesterday I started my first Agar to grain. Furthermore I inoculated two grain jars with a mycelium syringe of an oyster mushroom bought from a laboratory for mushroom cultivation, they supply big growing companies so I think it's 100% sterile
Here is one rye jar for example, one which I inoculated with the mycelium syringe.


But I have some questions:
1. Is it normal, that there are so many water drops on the glas? Is my humidity to high in the jars? I let the rye dry for one hour after boiling/simmering and shaked it many times during drying. Then I PCd the jars.
2. On the picutre I marked one rye berry with a red circle. The rye berry sticks to the glas a bit under the lid, far away from the rest of the rye berries (can't be seen well on the picture). It seems that there is some white stuff on it, could be mold, because it's a bit hairy. Better to toss the jar away now or shall I wait some days to see what will happen?
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23783055 - 10/29/16 03:58 PM (7 years, 3 months ago) |
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1. How long did you boil it? I have this issue with grain and spawn bags sometimes.
2. Could be oyster mycelium. Wait a few more days to be sure. Did you shake the jar after agar inoculation? If it is so, it starts to colonize on different locations inside the jar.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23783071 - 10/29/16 04:04 PM (7 years, 3 months ago) |
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I soaked them in water for about 18 hours, then I put the pot directly on the stove without changing the water they were soaked in.
I let the water boil and then reduced the heat so that it simmered, I let it simmer for about 10 Minutes, not more. As I saw that some berries are popping, I directly took the pot from the stove.
Then I put the rye into a screen, let it there for about an hour and shaked it every 5 minutes to get all the water out.
After that I put some berries on a paper towel and there was no water after removing them. So they seem to be perfect, therefore I put them in the jars and PCd them for 2 hours (not only 90 minutes, because german PC aren't able to reach 15psi).
Shouldn't there be any water on the glas?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23783090 - 10/29/16 04:10 PM (7 years, 3 months ago) |
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Sounds good. I like to spread the hot grain over a big surface. This way the grain dries very fast. Some moisture is absorbed by the grain after some days. It's the same when you inoculate with liquid culture. 1-2cc makes the grain look too wet but it gets absorbed after some time.
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23783124 - 10/29/16 04:27 PM (7 years, 3 months ago) |
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Ok fine, so I'll wait some days
Actually I didn't see any "problem", just wanted to update my nooblog
I put my jars in a clear tote (same like my SAB), it's in a room, that has about 20°C (68 F) and is quite still (considering air movement). I hope that's fine 
EDIT: After inoculation with the agar wedge, I knot that I should not shake, just take care, that the agar wedge is covered by grains - did that!  But what shall I do with the jars inoculated with the MS Syringe? Unfortunately my SAB is not that high and the syringe is very big, so I wasn't able to shoot directy at the edge of the jar, so I shooted on the grain from above it (openin the lid just a crack). Shall I shake the grain jar diectly or let the myeclium from the syringe recover some days?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/29/16 06:28 PM)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23788528 - 10/31/16 12:55 PM (7 years, 2 months ago) |
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Yeah dude pretty lame I like to remember where I started, wasn't anything special living in the ghetto poor trying to stay sane
No super legit set up but that rhy bag got me off the food stamps amen. Don't mind having a good laugh at myself not that I've gone too far
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: filthyknees]
#23788693 - 10/31/16 02:02 PM (7 years, 2 months ago) |
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haha, great man 
To make a small update:
My agar plates are growing and growing, made some transfers and I'm awaiting a totally clean plate to make some tiger drops. Maybe in a week or so I'll be ready for that
But I have noticed on two of my first agar plates some "water drops" on the mycelium. Not on the lid of the petri dish, directly on the mycelium. Especially there, where the mycelium seem to be dense.
It appeared on these agar plates, that I inoculated 14 days ago and that seem very clean, but I did not take any transfer from them, because I choose other plates to do that. So I let these plates "grow out". They really look like very small waterdrops, but if I shake the plate a bit, they don't move or so. They realy don't look like bacteria oder mold, just like small drops.
Is it a problem? Contamn? Bad conditions for the mycelium?
Here's a picture.

Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
Edited by AlCapone2k (10/31/16 02:03 PM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23800113 - 11/04/16 09:01 AM (7 years, 2 months ago) |
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Hey guys,
a small update.
I now have 6 half quart jars (quart jars don't fit into my PC) full of rye - all sterilized.
My agar work is also going on, I was able to "produce" two clean agar cultures after three transfers of B+.
Now they seem really clean and show a nice rizomorphic growth.
Is it possible to transfer wedges from these two plates to grain jars now or shall I better wait some days more until I inoculate jars with them? If it's ok, how many jars can I inoculate with one plate?
And if I better wait, is it ok to store sterilized rye jars without inoculating for some days or do they have to be inoculated immediately after sterilization?

Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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enlightenment
alchemist


Registered: 08/09/09
Posts: 1,647
Loc: Europe
Last seen: 8 months, 21 days
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
#23800317 - 11/04/16 10:57 AM (7 years, 2 months ago) |
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Storing sterilized jars for some days is OK.
You can inoculate with a colonized piece of your agar dish right now. But I often wait some more time. I like to use bigger pieces of agar.

I prefer to use at least the size of the marked '1/4'. The most time I use about the half plate for a 'quart' jar (a little bigger than a quart -> 1100ml jar).
You get more inoculation points with bigger pieces of agar.
Edited by enlightenment (11/04/16 11:04 AM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: enlightenment]
#23800734 - 11/04/16 01:54 PM (7 years, 2 months ago) |
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Thank you very much. If it's ok to wait some days before inoculation, then I better wait until more of the plate is colonized by the mycelium
Do you think the plates are clean enough or shall I better make another transfer?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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