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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
    #23766568 - 10/24/16 11:58 AM (7 years, 3 months ago)

Quote:

thebug76 said:
All three look clean. Me personally, I would go with red. Either would be sufficient, but newer growth tends to be more vigorous. Then I would get one from around the bottom and from the upper left as well.





What do you think of that?



So I take three wedges out of one plate and transfer to three new plates. So I open the dish, cut a wedge, close the first dish, open the second, put it in, open the old dish again, cut again and so one.

Do I have to flame sterilize or take a new sterilized "one use"-Skalpel every cut or can I use one scalpel for all the three transfers as long as it touches only agar?



Quote:

blackout said:
I would have taken a clone of that growkit. It is a known fruiting strain which was presumably carefully selected.

Seems you were streaking the plates and having growth in many places. I would only dip the swab in the very centre, this way if any spots other than the centre show growth it would be unwanted growth and you know your SAB technique is not great, or that your plates were not sterile to start with. It also means it will grow outwards from the centre so its is easier to select a clean leading edge. Having many dots of growth means they will grow into each other. Instead of dipping swabs I have picked a little bit off with a sterile tweezers and placed it in the middle of a plate, I did this as my plates were quite moist and I was worried about it absorbing nutrients, though others say they have no issue.





Yes, I was streaking the plates, actually it was a suggestion from bodhisatta. Since in his agar tek he talks about centre inoculation I did it with some plates to. In future I will only inoculate to the center.

And to the clone thing: Yeah, actually before harvesting my first flush I thought I won't go into this hobby, so I didn't care. Then I totally regret that and as the second flush came, I asked in some thread here and they told me that cloning a 2nd-flush mushroom isn't that good, so I made sporeprints.

Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/24/16 12:03 PM)


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Offlinethebug76
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23766576 - 10/24/16 12:02 PM (7 years, 3 months ago)

Correct. And I would flame after every transfer, just to be safe. Hell I flame after every hole in my pf jars. I've had a lot of contam troubles tho, so I'm extra cautious.


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OfflinePortabellaFella 1
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
    #23766584 - 10/24/16 12:05 PM (7 years, 3 months ago)

Be sure to cool your scalpel in an uncolonized portion of the agar before cutting or you'll kill your myc


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I would like to acquire anything I don’t have


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
    #23766586 - 10/24/16 12:06 PM (7 years, 3 months ago)

Quote:

PortabellaFella 1 said:
Be sure to cool your scalpel in an uncolonized portion of the agar before cutting or you'll kill your myc




yes, of course, thank you :smile: I read the thread of stro about cleaning more than one time :smile:

But I ask myself about the best procedure: Skalpel in the SAB - take it out - flame it red hot - get it back to SAB and so on? Shall I wait a bit after getting my hand with the scalpel back to the SAB to let everything settle?

Quote:

thebug76 said:
Correct. And I would flame after every transfer, just to be safe. Hell I flame after every hole in my pf jars. I've had a lot of contam troubles tho, so I'm extra cautious.





Ok, fine. So I'll go for this plate now.

Unforunately I only have "one use"-Skalpels, they can not be flamed that good because they have plastic around the metal. But it's no problem, they cost 40 euro-cents so I can afford three of them :laugh:

I will buy a multi-use skalpel next time :smile:


And what shall I do with the other plates? Also transfer or better wait and see what happens to them? Maybe they are conti free so I can directy use them for rye? Suggestions?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/24/16 12:08 PM)


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Offlinethebug76
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23766595 - 10/24/16 12:08 PM (7 years, 3 months ago)

If they're different strains, I would make tranfers from them too. Otherwise, I would just hold on to them til you know you're happy with your next set.

Yea, if you're sure they're clean, transfer some wedges to grain jars. You could probably use the entire piece of agar, but I read somewhere (can't remember where right at the moment) that contams like to sneak in around the edges. So, I generally cut all the way around the inside about a quarter of an inch or so from the side, then cut that circle into wedges and use that, leaving outer edge of agar alone.


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Edited by thebug76 (10/24/16 12:14 PM)


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23766607 - 10/24/16 12:13 PM (7 years, 3 months ago)

No, they are made from the same sporeprint.

Ok, I let them rest to see what will happen.

If mycelium colonizes the whole agar, it will start to fruit. Can I then still take a wedge to colonize new ones or must it be done at least before it's going to fruit?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Offlinethebug76
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23766618 - 10/24/16 12:17 PM (7 years, 3 months ago)

I edited my last post to answer the question about rye, but not before you saw it I guess.

If it pins inside the plate, clone it. And yes you can still transfer with it.


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Offlinethebug76
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
    #23766628 - 10/24/16 12:18 PM (7 years, 3 months ago)

You already pretty smart on this stuff OP. It won't be long, I'll be asking you questions.


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: thebug76]
    #23766639 - 10/24/16 12:23 PM (7 years, 3 months ago)

Quote:

thebug76 said:
You already pretty smart on this stuff OP. It won't be long, I'll be asking you questions.





Oh, thank you very much :smile: Before I even bought one petri dish, I read almost all tutorials here, just to be sure to know everything so that I don't mess up. I'm still a student and have not that much money to start things over again and again because they fail :smile:

So I'm really happy that my first agar inoculation seem to be done good at the moment :smile:


Buddy, I like your small trays in your signature. I think this could be also a possibility for me, since I only have a small students appartment and not always time, place and effort to make a big monotub. Is this perculit on the bottom?


Quote:

thebug76 said:
Yea, if you're sure they're clean, transfer some wedges to grain jars. You could probably use the entire piece of agar, but I read somewhere (can't remember where right at the moment) that contams like to sneak in around the edges. So, I generally cut all the way around the inside about a quarter of an inch or so from the side, then cut that circle into wedges and use that, leaving outer edge of agar alone.




Ok, fine, I will do the transfer of plate nr. 4 in a couple of minutes, all the three transfers showed in the second transfer picture.


Thank for your help! :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/24/16 12:24 PM)


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Offlinethebug76
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23766644 - 10/24/16 12:27 PM (7 years, 3 months ago)

No problem. Good luck.


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23767083 - 10/24/16 02:52 PM (7 years, 3 months ago)

Ok, the transfering process was a big mess :laugh:

My SAB was so soapy and dark (it's evening here now) that I wasn't able to see well into my dishes. After opening them I cut out a wedge but wasn't able to pic it up.

Are you picking it up by stabing into it (like with a sword) or do you use the scalpel like a spoon?

At the end my plate nr. 4 is now totally disturbed and because I hade to lift it up with my hands to see the wedge, it will have contamns for sure :laugh:

But anyway, next time I'll do better and prepare better.


Unfortunaetely I also saw, that there werde mold and bacteria in some of my NOT INOCULATED poured agar dishes. Don't know what has happened there but it seems the saran wrap didn't seal that well or something, because the dishes of the same day parafilmed are still good.


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisiblebodhisattaMDiscordReddit
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AgarStudent]
    #23767112 - 10/24/16 03:01 PM (7 years, 3 months ago)

Quote:

AgarStudent said:
Hmm if you are serious about the hobby, I would recommend a laminar flow hood to avoid contaminations.  You can build one yourself for not that much.

I don't know how you can avoid contamination without one to be honest, but some people seem to do without them?




you absolutely do not need a flowhood for this hobby, a Still Air Box (SAB) is far more than sufficient for anyone that's not making 100s of jars of spawn a week

my whole pour agar tek is brought to you by a small 66q SAB.

https://www.shroomery.org/forums/usergallery.php/gallery/300678/imgpl/5/imgpp/all/folder/agar
that's my agar gallery
you can see how I started off pretty nooby and things got better,
all the agar there was done in a SAB. and it's all poured plates.

you should be able to get an idea of the progression of how things look as you take transfers

Do not worry about getting an isolate. just get some clean healthy looking growth and go with it.


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
    #23767183 - 10/24/16 03:21 PM (7 years, 3 months ago)

yeah, I watched your gallery, so far in agar work as you I'd really like to be. As you can read, I totally messed my first transfer :laugh:

Now I really hope that at least either one transfer petri will do it's job or at least the source petri is not that damaged that I can throw it away.

Next time I really need a light source next to my SAB, otherwise it won't work.


Bodhi, are you picking transfer wedges up by stabing into it (like with a sword) or do you use the scalpel like a spoon?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23767189 - 10/24/16 03:23 PM (7 years, 3 months ago)

let me know if you can't view these videos in germany





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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
    #23767490 - 10/24/16 04:56 PM (7 years, 3 months ago)

I can watch them, thank you. Answer was given, stabbing the wedge is ok, so I'll do that next time. Unfortunately I tried to pull out the wedge with the scalpel used like a spoon, almost impossible without messing around in the agar.


To the first Video: He is also lifting up the dishes and holding them towards his body to see the mycelium/place to cut well. So I also can do that with an open petri dish?
The Problem is my angle - when sittin in front of the SAB I have a very bad angle to the petri dishes lying on the towel. Better would be an angle from above the petri. To be able to see better I just lifted the petri dish as he does with the pasty plate.

Is it sterile enough or will the lifting cause contamns to get into the plate?


To the second video: Do you also drop the whole agar plate into the rye or do you cut a wedge out and put it in your jar? :smile:


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/24/16 04:57 PM)


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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23767529 - 10/24/16 05:07 PM (7 years, 3 months ago)

you can cut big wedges


you can practice with agar first so you don't fuck up when you get to using the plates that have precious mycelium on them


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23767566 - 10/24/16 05:19 PM (7 years, 3 months ago)

yeah, I think I should do that. It's ok, it was my first time transferring and I fucked up, next time will be better :P

I have to do a transfer of a oyster mushroom these days anyway, so I can practice it. If I mess up, it's no problem cause I can get into the shop and buy a new mycelium syringe for oysters, of course not for cubensis :laugh: So it hurts more if I mess up with them.

Now it's waiting time.

The wedges put onto the new plate should colonize the agar with mycelium within 7 days, right?


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23767587 - 10/24/16 05:25 PM (7 years, 3 months ago)

how many days depends on a lot of things, genetics, temperature, size of the petri dish, what kind of agar


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OfflineAlCapone2k
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: bodhisatta]
    #23767605 - 10/24/16 05:31 PM (7 years, 3 months ago)

Ok, thank you very much for all your help! You really helped me a lot.

Now I wait until my new plates colonize from the wedge. I'll update with pictures.


Have a nice day all of you :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflinefIsh in my head
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Re: NoobLog: Print -> Agar -> Rye Spawn -> CVG -> Mono [Re: AlCapone2k]
    #23768540 - 10/24/16 10:34 PM (7 years, 3 months ago)

I'm back in the hobby myself and trying agar for the first time.  I going for the long run since i'm trying to wake some spores printed in 2003 !

I trully felt like a noob trying to scrape like a clumsy mofo a very dry print. Seems to me that I took way too much time during the whole process. 

I'm following your thread because need to learn transfer form MS agar.  You are a week ahead of me but for some reason I feel all my shit will contam :smile:

good luck!


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