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Offlineafflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 9 months
Agar questions.
    #23765760 - 10/24/16 12:30 AM (7 years, 3 months ago)

So I got some agar in the yesterday, and I'm going to prep and sterilize it tomorrow. This is my first time using it, my question is how long does it normally take to see mycelium growth?


Side questions.

Hepa filter worth if I use a sab, to filter out the room before innocent?

And if done right what is the success ration when using a sab?

Thanks for your help.


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InvisiblePirateSwazey
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Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Agar questions. [Re: afflictis]
    #23765771 - 10/24/16 12:37 AM (7 years, 3 months ago)

Quote:

how long does it normally take to see mycelium growth?



Depends on how you're culturing. If you started with spores, typically 7-14 days. Transferring live mycelium, plates are generally ready to be used within a week from inoculation.

Quote:

Hepa filter worth if I use a sab, to filter out the room before innocent?



If you already have one, run it. If not, save that money and throw it towards a flowhood down the road.

Quote:

And if done right what is the success ration when using a sab?



SAB works fine for plate work, it's just a pain in the ass pouring and managing plates with your arms stuck in a plastic box.


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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: Agar questions. [Re: afflictis]
    #23765775 - 10/24/16 12:38 AM (7 years, 3 months ago)

Lower sporeload in the room will always help, but a hepa is not necessary. Success rate of transfers should be around 99% or better. This will get your actual success up to 90% or better.


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Invisibleirequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
Re: Agar questions. [Re: Pastywhyte]
    #23765788 - 10/24/16 12:49 AM (7 years, 3 months ago)

Incubating at what temp?


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Agar questions. [Re: irequirechocolate]
    #23765813 - 10/24/16 01:15 AM (7 years, 3 months ago)

Don't incubate. Room temp is good.


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Offlinethebug76
2 years in.
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Registered: 05/31/15
Posts: 557
Loc: North of the equater and ...
Last seen: 3 months, 26 days
Re: Agar questions. [Re: Pastywhyte]
    #23765975 - 10/24/16 05:34 AM (7 years, 3 months ago)

:whathesaid:  listen to him. I just pay attention to what he, as well as other experienced shroomers, tells other people and I don't even have to ask questions anymore.


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