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Invisiblefungusflip
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Registered: 12/08/02
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cloning and karo
    #2376467 - 02/25/04 04:56 PM (12 years, 9 months ago)

first of all can a piece of tissue from a mushroom be dropped into karo water and expected to colonize? if so how long will this tissue remain good?


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InvisibleMilkVein
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Re: cloning and karo [Re: fungusflip]
    #2376544 - 02/25/04 05:18 PM (12 years, 9 months ago)

yes...but you will need to get a glove box


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Invisiblefungusflip
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Registered: 12/08/02
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Re: cloning and karo [Re: MilkVein]
    #2376585 - 02/25/04 05:31 PM (12 years, 9 months ago)

is it really that sensitive? i've done tons of grain to grain innoculations in my bathroom in the open still air and never had a problem with contamination at all. do you think it would be unwise to clone with a clean still air environment in my bathroom? is this possible without a glove box?


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let it be know, there is a fountain that was not made by the hands of men.


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InvisibleMilkVein
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Re: cloning and karo [Re: fungusflip]
    #2376681 - 02/25/04 06:10 PM (12 years, 9 months ago)

you will have to cut back to a piece of tissue that is sterile.


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But I'm all alone at last.
Rolling home to you.


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OfflineGr0wer
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Registered: 09/16/03
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Re: cloning and karo [Re: fungusflip]
    #2376802 - 02/25/04 07:27 PM (12 years, 9 months ago)

you dont need a glovebox, you can do it in your bathroom as you said with only a slight chance of contam. I do GTG in my room, i take out everything walk out for a few hours to let teh air still then walk in slowly ect. Make sure you and wear a bandana over your mouth and nose to stop any drafts. I also did 2 jars of single grain to karo trasfer a few weeks ago in my still basment and it came out contam free. To stay on the safe side i would do more then one jar just in case of contam. Dont cut the stem tear it then cut off a chunk out of the center. Cutting down the stem will bring conams in on the blade when you cut the skin


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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