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mushhiehunter
Trichodermatologist


Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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What now?
#23763922 - 10/23/16 11:03 AM (7 years, 3 months ago) |
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Ok so I got some rye colonizing right now with MS and also some agar plates made from MS which are about ready for first transfer. Now is my best bet to grow the MS grains and select the best mushroom and isolate it or take the route of risk and isolate best looking strain on agar and have the next batch inoculated with it? What are the chances of good looking isolated strain to be a shit fruiter? 50% 80%? Any input appreciated
Edited by mushhiehunter (10/23/16 11:04 AM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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I would grow the Ms out and clone the best looking fruits in the meantime continue to isolate on your Ms agar.
Cloning gets you closer to sectoring it takes many transfers to successful isolate a varity of cubes. As far as I have read you got about 1 in 40 (plate wise) of a good isolate. Keep in mind thats not the awesome amazing isolate but a good decent one.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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mushhiehunter
Trichodermatologist


Registered: 10/11/16
Posts: 837
Loc: Česká republika
Last seen: 3 years, 2 months
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Quote:
van hatton said: I would grow the Ms out and clone the best looking fruits in the meantime continue to isolate on your Ms agar.
Cloning gets you closer to sectoring it takes many transfers to successful isolate a varity of cubes. As far as I have read you got about 1 in 40 (plate wise) of a good isolate. Keep in mind thats not the awesome amazing isolate but a good decent one.
OK, at least I got some experience in the agar field and I know what to do and what not to do so when the giant-ass mushroom comes, I'll be ready 
I guess I will use the agar jars to make some extra LC just to be on the safe side. Multi spore LC is still better than no spore LC
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Quote:
van hatton said: I would grow the Ms out and clone the best looking fruits in the meantime continue to isolate on your Ms agar.
right on. we all know that the waiting game can sometimes be frustrating so while ive been cleaning/isolating plates ive kept MS grows going so i have something to look at as well as getting clones and growing those out.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: What now? [Re: mushboy]
#23764077 - 10/23/16 11:54 AM (7 years, 3 months ago) |
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Quote:
I guess I will use the agar jars to make some extra LC just to be on the safe side. Multi spore LC is still better than no spore LC 
Lol I think I did that right but be careful with Ms LC no spore print or syringe is 100% clean as fruits are not grown in a sterile environment. But hey if you got it too work more power to ya.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: What now? [Re: mushboy]
#23764087 - 10/23/16 11:58 AM (7 years, 3 months ago) |
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Quote:
mushboy said:
Quote:
van hatton said: I would grow the Ms out and clone the best looking fruits in the meantime continue to isolate on your Ms agar.
right on. we all know that the waiting game can sometimes be frustrating so while ive been cleaning/isolating plates ive kept MS grows going so i have something to look at as well as getting clones and growing those out.
Yeah its nice to have something to check on and keep you busy. Expanding your horizon is never bad
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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