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just_curious
Cultivator



Registered: 03/15/14
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Want to try my hand at DFA (will this brand work?)
#23761644 - 10/22/16 05:42 PM (7 years, 3 months ago) |
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Okay, so I've been looking at DFA for a while and see some conflicting stuff. At the end of the day, what it boiled down to in every post was "use the good stuff". Which I definitely buy for my dogs. The shit is like $45 for 20lbs or something. The problem is, I read the back and see that meats are the first ingredient, followed by whole flour, followed by grains. The meat is what scares me but the rest seems decent (until you get lower in the list). But then, I see the guaranteed nutrients in the bottom left of the pic, and they all sound like good added benefits? Could you let me know what you guys think? I was gonna do 5 grams agar, to 2.5-3 grams crushed dog food, to 1.8 grams honey, to 1 cup water for a total of 16-20 dishes. I just don't want to waste supplies if somebody is like "nope, not a good brand" lol
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
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Re: Want to try my hand at DFA (will this brand work?) [Re: just_curious]
#23761737 - 10/22/16 06:05 PM (7 years, 3 months ago) |
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I think it'll work fine.
I usually use the cheapest I can find which usually has corn as the first ingredient and then meat fat/ meal/ etc... usually has a bunch of other crap and other grains and veggies too. works great for me
the recipe i use is 10g agar to 8g dog food to 500 ml water I use less nutrition for faster more rhizo growth as preference.
you can use 10g dog food for that recipe and it works fine especially for spore germination.
I've also seen people say they use grain free dog food with great success.
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Edited by tombosley8 (10/22/16 06:06 PM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Want to try my hand at DFA (will this brand work?) [Re: tombosley8]
#23763215 - 10/23/16 04:34 AM (7 years, 3 months ago) |
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ANY will be fine. Doesn't have to be good stuff.
I used to use a better brand but only because my actual dog liked it. Then i moved and couldn't get it anymore so I switched to regular old Purina dog chow (green bag) and its fine, if not better because the color of the plates comes out a really opaque orange (if u grind the kibble and don't strain it before PCing)
8-10g/500ml is fine like Tom said. unless u cut down the DF don't add more shit. As you have seen on the label, it already contains a multitude of nutrients the mycelium can draw from
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Want to try my hand at DFA (will this brand work?) [Re: blindingleaf]
#23763225 - 10/23/16 04:47 AM (7 years, 3 months ago) |
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why DFA??
do you have any experience with MEA? it is the standard for fungi, dirt cheap, and i cant really see any reason to use something as nutrient dense as dog food. i know some people do, and have for a long time, but many more use MEA.
dog food has a lot of stuff in it that is not necessary, or even available to the myc we are culturing, but might very well be available to contams
IME, half strength MEA is great for encouraging rhizo growth, and full strength for long term storage stuff. when i add yeast or peptone, the growth gets very dense, in a bad way.
pretty much anything will work, but it makes sense to me to go with the least nutrition density you can get away with, only adding a small amount of a simple sugar, rather than a broad spectrum of nutrients more suited to dog nutrition than cubensis myc
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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blindingleaf
blue collar underworld


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Re: Want to try my hand at DFA (will this brand work?) [Re: c10h12n2o]
#23763251 - 10/23/16 05:28 AM (7 years, 3 months ago) |
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Dog food is cheaper by weight and much more accesible. I can't find malt in the hood where I live but I can find dog kibble at any corner store/gas station/etc.
I use MEA too. The contam risk is the same for all media unless you consider sterilization time.
We are protected by the covenant of sterile technique
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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c10h12n2o
serial dilutor



Registered: 01/21/15
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Re: Want to try my hand at DFA (will this brand work?) [Re: blindingleaf]
#23763276 - 10/23/16 05:48 AM (7 years, 3 months ago) |
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makes sense. amen to that brother!
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
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Re: Want to try my hand at DFA (will this brand work?) [Re: c10h12n2o]
#23763277 - 10/23/16 05:50 AM (7 years, 3 months ago) |
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-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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just_curious
Cultivator



Registered: 03/15/14
Posts: 1,225
Loc: 'Merica (more spcifically...
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Re: Want to try my hand at DFA (will this brand work?) [Re: tombosley8]
#23766867 - 10/24/16 01:45 PM (7 years, 3 months ago) |
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Quote:
tombosley8 said: I think it'll work fine.
I usually use the cheapest I can find which usually has corn as the first ingredient and then meat fat/ meal/ etc... usually has a bunch of other crap and other grains and veggies too. works great for me
the recipe i use is 10g agar to 8g dog food to 500 ml water I use less nutrition for faster more rhizo growth as preference.
you can use 10g dog food for that recipe and it works fine especially for spore germination.
I've also seen people say they use grain free dog food with great success.
Sorry for the late response, been very busy the last couple days. How many plates did this recipe make for you? I use pasty plates. Also, you don't use any honey or karo with your recipe? Last question, have you ever tried using food coloring with this recipe, or does the dog food make it pointless because it will be brown?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Want to try my hand at DFA (will this brand work?) [Re: just_curious] 1
#23767092 - 10/24/16 02:55 PM (7 years, 3 months ago) |
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if you make agar with meat/protein and some contamination grows on it, it might be a smart idea to wrap the plate extra well and toss it out. you can easily grow up astronomical numbers of human pathogenic bacteria. don't play microbiologist and make slides with shit that grew on a meat agar plate is my suggestion
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