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InvisibleHallucyjunkie99

Registered: 10/21/15
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Agar colonization question updated with pics
    #23761155 - 10/22/16 02:40 PM (7 years, 3 months ago)

Hey guys 5 of my pasty plates colonized and from the looks no contams. :wink: I'm wondering what percentage of colonization I should wait for to isolate a strain? (of fast rhizo growth) anybody got a pic so I know what it looks like ?

My plates aren't growing from the center more points popped up since this morning all the plates look like these 3 so where do I go from here ?



Edited by Hallucyjunkie99 (10/22/16 03:06 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761158 - 10/22/16 02:43 PM (7 years, 3 months ago)



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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761168 - 10/22/16 02:46 PM (7 years, 3 months ago)

Thank you enlightenment for enlightening me :stoned: that's around 45-50% correct ?


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761170 - 10/22/16 02:47 PM (7 years, 3 months ago)

You can transfer earlier. If you see sectors cut out the sector you like.


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761172 - 10/22/16 02:49 PM (7 years, 3 months ago)

Awesome thank you :thumbup:


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761393 - 10/22/16 04:31 PM (7 years, 3 months ago)

It's gonna take a while and a lot of plates to get an isolate from MS.


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Offlineenlightenment
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Re: Agar colonization question [Re: PortabellaFella 1]
    #23761423 - 10/22/16 04:44 PM (7 years, 3 months ago)

That's true. You can try to take a multispore fruit and clone it. Sometimes it is already an isolate (rare) but there are much less sectors present compared to MS->agar.


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OfflineAlCapone2k
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Re: Agar colonization question [Re: enlightenment]
    #23761655 - 10/22/16 05:44 PM (7 years, 3 months ago)

Same question here:

I have four agar plates colonizing from a spore print. Since now there's no contamination I can see.

The mycelium grows at the points of inoculation, but I can only see it when I hold the agar plate towards a lamp. It doesn't even look nearly that "dense" like on the picture.

I think I still have to wait some days before transferring to another plate, right?

Or is transferring not necessary, since I can't see any contamination yet?

Do I only have to transfer if I want to isolate or I have contamns, or shall I always transfer?


Regards


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Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Offlineenlightenment
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Re: Agar colonization question [Re: AlCapone2k]
    #23761681 - 10/22/16 05:52 PM (7 years, 3 months ago)

You should wait some days. At the beginning of growth mycelium is almost invisible. You can transfer to make backups of your ms culture(s).


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OfflineChristopherJohnson


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Re: Agar colonization question [Re: enlightenment]
    #23761695 - 10/22/16 05:55 PM (7 years, 3 months ago)

Not sure if you have already read this:
Stro's Cleaning and Isolating On Agar
A lot of really great info!! ^^


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OfflineAlCapone2k
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Re: Agar colonization question [Re: ChristopherJohnson]
    #23761736 - 10/22/16 06:05 PM (7 years, 3 months ago)

thank you for your answers.

Of youre I read it, even before I bought my first agar :smile:

I am reading a lot, sometimes weeks before starting the entire tek, but sometimes the Teks seem to be described for people who already have some experience, a noob doesn't understand everything. Furthermore my english is school-tought, so urban language is sometimes quite hard for me to understand.

In the mentioned thread he's on cleaning from dishes with contamns.

But my dishes don't have contamns yet, maybe there won't be any, maybe they'll come. I don't know yet.

But I always read: Never let your mycelium colonize too much of the agar, always transfer to other dishes.

But I ask myself: If it's not for isolation purpose, not for a backup and there are not contamns, why transferring and risking to get a contamn? :smile: Can't I just wait for the petri dish to fully colonize and if theres still no contamn, directly inoculate grain jars with a piece of agar or tiger drop? :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/22/16 06:07 PM)


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: ChristopherJohnson]
    #23761747 - 10/22/16 06:08 PM (7 years, 3 months ago)

so what your saying is I should make a few fresh plates with these agar to agar for a backup then do a ms grow clone some fruits to agar that leading to a sooner isolate?can I use some wedges to inoculate grains


Edited by Hallucyjunkie99 (10/22/16 06:19 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761776 - 10/22/16 06:17 PM (7 years, 3 months ago)

Of course you can use wedges to inoculate.
The problem with cloned fruits is senescence. But you should not worry about that as a newcomer. A lot of people have great success with clones or isolates of clones.


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761784 - 10/22/16 06:21 PM (7 years, 3 months ago)

OK I have no contams as of now  its been almost 6 days since innoc I'm gonna let colonize then do an agar to grain grow make some clones from a minimono and try to isolate them thank you


Edited by Hallucyjunkie99 (10/22/16 06:22 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761792 - 10/22/16 06:24 PM (7 years, 3 months ago)

:thumbup: You're welcome! If you work with agar you should be as fast and as clean as possible. Keep us posted. You could start a new grow log thread after you inoculated your grain jars. Good luck :wink:


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761797 - 10/22/16 06:27 PM (7 years, 3 months ago)

Will do


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761930 - 10/22/16 07:08 PM (7 years, 3 months ago)

Just remember, just because your myc looks clean, contams can still be hiding within it. It's safest to make multiple transfers and monitor.


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: PortabellaFella 1]
    #23762907 - 10/23/16 12:29 AM (7 years, 3 months ago)

How many transfers I have 12 more dishes ready to go


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23763703 - 10/23/16 09:58 AM (7 years, 3 months ago)

if you let the plates you have now grow out and they are clean to the best of your knowledge, do like enlightenment says. Wedge or tiger drop, grow,clone and use those plates to possibly isolate. If you wedge and keep the plate you wedged from, chances are it'll pin and you can clone that pin as well. It's a little faster than a full grow to clone.


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OfflineMushierage
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Re: Agar colonization question [Re: enlightenment]
    #23763743 - 10/23/16 10:11 AM (7 years, 3 months ago)

Quote:

enlightenment said:
That's true. You can try to take a multispore fruit and clone it. Sometimes it is already an isolate (rare) but there are much less sectors present compared to MS->agar.





This is the way to really getting good isolated genetics. I find that a ms tub generally gives at least one awesome clone, and senescence is not usually a problem for quite some time with that clone.  I will usually get the best out of five clone candidates, put a tissue sample to agar (from a torn apart stipe, and run my blade down the middle to scratch off an appreciable sample).  Ill take a couple plates, grow them, and transfer each sector into another plate.  By visual attrition I usually end up with 1 or 2 -great- fruiters this way that are always pretty damned potent.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAlCapone2k
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Re: Agar colonization question [Re: Mushierage]
    #23763945 - 10/23/16 11:10 AM (7 years, 3 months ago)

I have inoculated my agar plates with a self-made spore print about 6 days ago.

The mycelium grow is now visible with the eye without holding the petri dish towards a lamp.

Since now I can't see any contamns, around the mycelium everything looks fine and also the free space of agar doesn't show any strange look.

Is it possible that I made a sporeprint to agar inoculation without contamination? It wonders, cause I always read that there is no contamn free sporeprint.


Another question:

If I let my dish colonize until it's time for rye inoculation, I also can make a transfer of another wedge too. But cutting out a wege for rye + cutting out a wedge for transfer + removing them from the old plate = a lot of time for contamination to settle either on the old petri dish or on the wedges. How do you do that?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Invisibleamidogen
see you on the other side

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Re: Agar colonization question [Re: AlCapone2k]
    #23763963 - 10/23/16 11:15 AM (7 years, 3 months ago)

.


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The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:04 PM)


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OfflineMushierage
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Re: Agar colonization question [Re: AlCapone2k]
    #23763982 - 10/23/16 11:19 AM (7 years, 3 months ago)

If you intend to transfer the culture (which you should) then do it as early as possible.  Don't give contamination any time to sprout up from the ms inoculation because believe it or not, you can rest assured that there are some type of bacterial spores laden within your ms, to whatever degree that may be. 

Take several transfers.  Don't put all your eggs in one basket.  It'll be worth the wait and effort.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAlCapone2k
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Re: Agar colonization question [Re: Mushierage]
    #23764020 - 10/23/16 11:28 AM (7 years, 3 months ago)

Ok, thanks guys, I think you are right. I don't think that I am the first person who makes an agar inoculation the first time in his life with a spore print without any contamn :P

I think I still wait one day and then I'll post a pic here of my agar plates, maybe you can tell me whether to make a transfer or wait.


But I ask myself: If I make a transfer to another plate, can I still let the first plate colonize further or is it for the trash?


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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OfflineMushierage
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Re: Agar colonization question [Re: AlCapone2k]
    #23764136 - 10/23/16 12:20 PM (7 years, 3 months ago)

Absolutely you can let the original plate grow out, and get further use out of it.

If it looks healthy, tiger drop it into some grain, or take several wedges from it from clean sections (probably a smarter idea) and put those to grain so you aren't unintentionally dropping in a load of bacteria.  But you almost never get a completely clean plate when you started that plate with ms which is why transferring is highly recommended.

Pics of your plates will definitely help.  Anybody here with agar experience should be able to offer you guidance. 

Also when transferring and inoculating, use Mad Seasons guide to winning with agar Here.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAlCapone2k
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Re: Agar colonization question [Re: Mushierage]
    #23764199 - 10/23/16 12:47 PM (7 years, 3 months ago)

thank you very much, I'll read this thread again :smile:

Tomorrow I'll give you some pics :wink:


Regards


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Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: AlCapone2k]
    #23764221 - 10/23/16 12:58 PM (7 years, 3 months ago)

I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up

Can I use any knife to transfer agar to agar my plates are 70% colonized so I guess I better transfer and start some grain jars I don't see bacteria on my plates or any trich I know hidden contams exist so I will see with 2 QTS if I had any and make around 12 transfers out of the 6 I have


Edited by Hallucyjunkie99 (10/23/16 01:02 PM)


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OfflineMushierage
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23764229 - 10/23/16 01:00 PM (7 years, 3 months ago)

Quote:

Hallucyjunkie99 said:
I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up





Didn't even realize that he wasn't the OP.  Yeah, don't thread jack.  Start your own thread dude.


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Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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OfflineAlCapone2k
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23764340 - 10/23/16 01:53 PM (7 years, 3 months ago)

Quote:

Hallucyjunkie99 said:
I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up






sorry mate, of course I'm going to open my own thread with the pics, the questions I had asked were similiar to your questions so I thought they were ok.


--------------------
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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: AlCapone2k]
    #23767680 - 10/24/16 05:54 PM (7 years, 3 months ago)

So can I use any knife


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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23767701 - 10/24/16 06:01 PM (7 years, 3 months ago)

Quote:

Hallucyjunkie99 said:
So can I use any knife





Theoretically if it gets red hot yes,

But get an xacto knife if you cant get a scalpel

You can thank me later


Edited by spore-ty (10/24/16 06:02 PM)


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: spore-ty]
    #23767715 - 10/24/16 06:06 PM (7 years, 3 months ago)

I have a blowtorch so it gets red hot I looked for an xacto today but wasn't in stock so a steak knife it is lol


Edited by Hallucyjunkie99 (10/24/16 06:07 PM)


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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23768649 - 10/24/16 11:39 PM (7 years, 3 months ago)

Quote:

Hallucyjunkie99 said:
I have a blowtorch so it gets red hot I looked for an xacto today but wasn't in stock so a steak knife it is lol





Haha nice man well I suppose in my best liam neeson voice goodluck haha


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OfflineAlCapone2k
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Re: Agar colonization question [Re: spore-ty]
    #23768939 - 10/25/16 03:56 AM (7 years, 3 months ago)

Get Scalpel No. 7 :smile: and Blades Nr. 11 :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: AlCapone2k]
    #23775417 - 10/27/16 01:23 AM (7 years, 3 months ago)

Quote:

AlCapone2k said:
Get Scalpel No. 7 :smile: and Blades Nr. 11 :smile:



cant i buy any x-acto knife pen or does it need to be #11


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