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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar colonization question [Re: Mushierage]
#23763945 - 10/23/16 11:10 AM (7 years, 3 months ago) |
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I have inoculated my agar plates with a self-made spore print about 6 days ago.
The mycelium grow is now visible with the eye without holding the petri dish towards a lamp.
Since now I can't see any contamns, around the mycelium everything looks fine and also the free space of agar doesn't show any strange look.
Is it possible that I made a sporeprint to agar inoculation without contamination? It wonders, cause I always read that there is no contamn free sporeprint.
Another question:
If I let my dish colonize until it's time for rye inoculation, I also can make a transfer of another wedge too. But cutting out a wege for rye + cutting out a wedge for transfer + removing them from the old plate = a lot of time for contamination to settle either on the old petri dish or on the wedges. How do you do that?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Agar colonization question [Re: AlCapone2k]
#23763963 - 10/23/16 11:15 AM (7 years, 3 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:04 PM)
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Agar colonization question [Re: AlCapone2k]
#23763982 - 10/23/16 11:19 AM (7 years, 3 months ago) |
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If you intend to transfer the culture (which you should) then do it as early as possible. Don't give contamination any time to sprout up from the ms inoculation because believe it or not, you can rest assured that there are some type of bacterial spores laden within your ms, to whatever degree that may be.
Take several transfers. Don't put all your eggs in one basket. It'll be worth the wait and effort.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar colonization question [Re: Mushierage]
#23764020 - 10/23/16 11:28 AM (7 years, 3 months ago) |
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Ok, thanks guys, I think you are right. I don't think that I am the first person who makes an agar inoculation the first time in his life with a spore print without any contamn :P
I think I still wait one day and then I'll post a pic here of my agar plates, maybe you can tell me whether to make a transfer or wait.
But I ask myself: If I make a transfer to another plate, can I still let the first plate colonize further or is it for the trash?
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Agar colonization question [Re: AlCapone2k]
#23764136 - 10/23/16 12:20 PM (7 years, 3 months ago) |
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Absolutely you can let the original plate grow out, and get further use out of it.
If it looks healthy, tiger drop it into some grain, or take several wedges from it from clean sections (probably a smarter idea) and put those to grain so you aren't unintentionally dropping in a load of bacteria. But you almost never get a completely clean plate when you started that plate with ms which is why transferring is highly recommended.
Pics of your plates will definitely help. Anybody here with agar experience should be able to offer you guidance.
Also when transferring and inoculating, use Mad Seasons guide to winning with agar Here.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar colonization question [Re: Mushierage]
#23764199 - 10/23/16 12:47 PM (7 years, 3 months ago) |
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thank you very much, I'll read this thread again
Tomorrow I'll give you some pics
Regards
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: Agar colonization question [Re: AlCapone2k]
#23764221 - 10/23/16 12:58 PM (7 years, 3 months ago) |
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I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up
Can I use any knife to transfer agar to agar my plates are 70% colonized so I guess I better transfer and start some grain jars I don't see bacteria on my plates or any trich I know hidden contams exist so I will see with 2 QTS if I had any and make around 12 transfers out of the 6 I have
Edited by Hallucyjunkie99 (10/23/16 01:02 PM)
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Quote:
Hallucyjunkie99 said: I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up
Didn't even realize that he wasn't the OP. Yeah, don't thread jack. Start your own thread dude.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Quote:
Hallucyjunkie99 said: I'm not trying to be rude but can you start your own thread with your pics and questions I'm just getting jumbled up
sorry mate, of course I'm going to open my own thread with the pics, the questions I had asked were similiar to your questions so I thought they were ok.
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: Agar colonization question [Re: AlCapone2k]
#23767680 - 10/24/16 05:54 PM (7 years, 3 months ago) |
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So can I use any knife
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
Hallucyjunkie99 said: So can I use any knife
Theoretically if it gets red hot yes,
But get an xacto knife if you cant get a scalpel
You can thank me later
Edited by spore-ty (10/24/16 06:02 PM)
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: Agar colonization question [Re: spore-ty]
#23767715 - 10/24/16 06:06 PM (7 years, 3 months ago) |
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I have a blowtorch so it gets red hot I looked for an xacto today but wasn't in stock so a steak knife it is lol
Edited by Hallucyjunkie99 (10/24/16 06:07 PM)
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spore-ty



Registered: 01/21/16
Posts: 1,028
Loc: In the bush
Last seen: 2 years, 10 months
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Quote:
Hallucyjunkie99 said: I have a blowtorch so it gets red hot I looked for an xacto today but wasn't in stock so a steak knife it is lol
Haha nice man well I suppose in my best liam neeson voice goodluck haha
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Re: Agar colonization question [Re: spore-ty]
#23768939 - 10/25/16 03:56 AM (7 years, 3 months ago) |
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Get Scalpel No. 7 and Blades Nr. 11
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Hallucyjunkie99

Registered: 10/21/15
Posts: 132
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Re: Agar colonization question [Re: AlCapone2k]
#23775417 - 10/27/16 01:23 AM (7 years, 3 months ago) |
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Quote:
AlCapone2k said: Get Scalpel No. 7 and Blades Nr. 11 
cant i buy any x-acto knife pen or does it need to be #11
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