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InvisibleHallucyjunkie99

Registered: 10/21/15
Posts: 132
Agar colonization question updated with pics
    #23761155 - 10/22/16 02:40 PM (7 years, 3 months ago)

Hey guys 5 of my pasty plates colonized and from the looks no contams. :wink: I'm wondering what percentage of colonization I should wait for to isolate a strain? (of fast rhizo growth) anybody got a pic so I know what it looks like ?

My plates aren't growing from the center more points popped up since this morning all the plates look like these 3 so where do I go from here ?



Edited by Hallucyjunkie99 (10/22/16 03:06 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761158 - 10/22/16 02:43 PM (7 years, 3 months ago)



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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761168 - 10/22/16 02:46 PM (7 years, 3 months ago)

Thank you enlightenment for enlightening me :stoned: that's around 45-50% correct ?


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761170 - 10/22/16 02:47 PM (7 years, 3 months ago)

You can transfer earlier. If you see sectors cut out the sector you like.


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761172 - 10/22/16 02:49 PM (7 years, 3 months ago)

Awesome thank you :thumbup:


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761393 - 10/22/16 04:31 PM (7 years, 3 months ago)

It's gonna take a while and a lot of plates to get an isolate from MS.


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I would like to acquire anything I don’t have


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Offlineenlightenment
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Re: Agar colonization question [Re: PortabellaFella 1]
    #23761423 - 10/22/16 04:44 PM (7 years, 3 months ago)

That's true. You can try to take a multispore fruit and clone it. Sometimes it is already an isolate (rare) but there are much less sectors present compared to MS->agar.


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OfflineAlCapone2k
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Re: Agar colonization question [Re: enlightenment]
    #23761655 - 10/22/16 05:44 PM (7 years, 3 months ago)

Same question here:

I have four agar plates colonizing from a spore print. Since now there's no contamination I can see.

The mycelium grows at the points of inoculation, but I can only see it when I hold the agar plate towards a lamp. It doesn't even look nearly that "dense" like on the picture.

I think I still have to wait some days before transferring to another plate, right?

Or is transferring not necessary, since I can't see any contamination yet?

Do I only have to transfer if I want to isolate or I have contamns, or shall I always transfer?


Regards


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


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Offlineenlightenment
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Re: Agar colonization question [Re: AlCapone2k]
    #23761681 - 10/22/16 05:52 PM (7 years, 3 months ago)

You should wait some days. At the beginning of growth mycelium is almost invisible. You can transfer to make backups of your ms culture(s).


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OfflineChristopherJohnson


Registered: 10/02/16
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Re: Agar colonization question [Re: enlightenment]
    #23761695 - 10/22/16 05:55 PM (7 years, 3 months ago)

Not sure if you have already read this:
Stro's Cleaning and Isolating On Agar
A lot of really great info!! ^^


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OfflineAlCapone2k
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Re: Agar colonization question [Re: ChristopherJohnson]
    #23761736 - 10/22/16 06:05 PM (7 years, 3 months ago)

thank you for your answers.

Of youre I read it, even before I bought my first agar :smile:

I am reading a lot, sometimes weeks before starting the entire tek, but sometimes the Teks seem to be described for people who already have some experience, a noob doesn't understand everything. Furthermore my english is school-tought, so urban language is sometimes quite hard for me to understand.

In the mentioned thread he's on cleaning from dishes with contamns.

But my dishes don't have contamns yet, maybe there won't be any, maybe they'll come. I don't know yet.

But I always read: Never let your mycelium colonize too much of the agar, always transfer to other dishes.

But I ask myself: If it's not for isolation purpose, not for a backup and there are not contamns, why transferring and risking to get a contamn? :smile: Can't I just wait for the petri dish to fully colonize and if theres still no contamn, directly inoculate grain jars with a piece of agar or tiger drop? :smile:


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek


Edited by AlCapone2k (10/22/16 06:07 PM)


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InvisibleHallucyjunkie99

Registered: 10/21/15
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Re: Agar colonization question [Re: ChristopherJohnson]
    #23761747 - 10/22/16 06:08 PM (7 years, 3 months ago)

so what your saying is I should make a few fresh plates with these agar to agar for a backup then do a ms grow clone some fruits to agar that leading to a sooner isolate?can I use some wedges to inoculate grains


Edited by Hallucyjunkie99 (10/22/16 06:19 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761776 - 10/22/16 06:17 PM (7 years, 3 months ago)

Of course you can use wedges to inoculate.
The problem with cloned fruits is senescence. But you should not worry about that as a newcomer. A lot of people have great success with clones or isolates of clones.


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InvisibleHallucyjunkie99

Registered: 10/21/15
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Re: Agar colonization question [Re: enlightenment]
    #23761784 - 10/22/16 06:21 PM (7 years, 3 months ago)

OK I have no contams as of now  its been almost 6 days since innoc I'm gonna let colonize then do an agar to grain grow make some clones from a minimono and try to isolate them thank you


Edited by Hallucyjunkie99 (10/22/16 06:22 PM)


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Offlineenlightenment
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761792 - 10/22/16 06:24 PM (7 years, 3 months ago)

:thumbup: You're welcome! If you work with agar you should be as fast and as clean as possible. Keep us posted. You could start a new grow log thread after you inoculated your grain jars. Good luck :wink:


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: enlightenment]
    #23761797 - 10/22/16 06:27 PM (7 years, 3 months ago)

Will do


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23761930 - 10/22/16 07:08 PM (7 years, 3 months ago)

Just remember, just because your myc looks clean, contams can still be hiding within it. It's safest to make multiple transfers and monitor.


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InvisibleHallucyjunkie99

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Re: Agar colonization question [Re: PortabellaFella 1]
    #23762907 - 10/23/16 12:29 AM (7 years, 3 months ago)

How many transfers I have 12 more dishes ready to go


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OfflinePortabellaFella 1
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Re: Agar colonization question [Re: Hallucyjunkie99]
    #23763703 - 10/23/16 09:58 AM (7 years, 3 months ago)

if you let the plates you have now grow out and they are clean to the best of your knowledge, do like enlightenment says. Wedge or tiger drop, grow,clone and use those plates to possibly isolate. If you wedge and keep the plate you wedged from, chances are it'll pin and you can clone that pin as well. It's a little faster than a full grow to clone.


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OfflineMushierage
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Re: Agar colonization question [Re: enlightenment]
    #23763743 - 10/23/16 10:11 AM (7 years, 3 months ago)

Quote:

enlightenment said:
That's true. You can try to take a multispore fruit and clone it. Sometimes it is already an isolate (rare) but there are much less sectors present compared to MS->agar.





This is the way to really getting good isolated genetics. I find that a ms tub generally gives at least one awesome clone, and senescence is not usually a problem for quite some time with that clone.  I will usually get the best out of five clone candidates, put a tissue sample to agar (from a torn apart stipe, and run my blade down the middle to scratch off an appreciable sample).  Ill take a couple plates, grow them, and transfer each sector into another plate.  By visual attrition I usually end up with 1 or 2 -great- fruiters this way that are always pretty damned potent.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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