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Offlinekeifnnugs
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Registered: 11/27/15
Posts: 335
Last seen: 2 months, 27 days
Testing isolates
    #23760394 - 10/22/16 09:51 AM (8 years, 2 months ago)

Was wondering what the best methods are for testing isolates..
Say you have 8 pasty plates that aren't say 100% true isolates that you wanted to test is there a smaller way to test them instead of doing 8 66qt monos?
Would mini monos be better or the bottle tek better?  What options are there?

Also would it be better to run MS->66qt mono and clone the best or would running the MS on agar-> multiple transfers-> mono be better?

If there is a thread on this I may have missed when running through the search please let me know

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Invisibledankington
The Stranger
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Folding@home Statistics
Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: Testing isolates [Re: keifnnugs]
    #23760481 - 10/22/16 10:24 AM (8 years, 2 months ago)

Mini mono or bottle grows would work. Just keep in mind, a culture that does well in a bottle, may not perform as well on a bulk sub, and vice versa.

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