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irequirechocolate
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Registered: 10/15/16
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Lab access
#23758760 - 10/21/16 06:17 PM (7 years, 3 months ago) |
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Since I joined this board about a week ago, I've only posted a few questions about my basic, first-time PF TEK setup, but it seems to be going well enough that I'm already thinking about what I might want to try in the future. I am a geneticist/microbiologist that works in a lab and so I have access to a lot of resources most people don't. For example, I was able to use an autoclave when sterilizing my cakes and some more simple things like a clean bench w/ a flame and a sharps container for syringe disposal, etc.
My first question is, if you were in my shoes and additionally had access to an incubator (30°C, aka 86°F, unfortunately too hot for any tek I've read about), a fume hood, basic micro tools and supplies, access to lots of high grade reagent vendors, etc, what would you do to step up your cultivation game?
Also, because I have access to a lot of scientific literature, I've been perusing some old papers. Thing is, they truly are OLD. So, my second question is, as an experienced cultivator, what type of questions would you like to see geneticists answer? (I already have a lot of work on my plate, but I am still curious what this community wonders about).
EDIT: If this is your first time looking at this thread, you may want to skip to ~the halfway mark of page 2 to avoid the arguments
Edited by irequirechocolate (10/21/16 10:54 PM)
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
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I want to start off by pointing out that I am NOT an experienced cultivator, but one of the first questions that comes to mind for me is: do you have access to a gas chromatograph? I feel like there are lots of fun results from experiments that would best be measured via GC.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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irequirechocolate
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Quote:
r.lutece said: I want to start off by pointing out that I am NOT an experienced cultivator, but one of the first questions that comes to mind for me is: do you have access to a gas chromatograph? I feel like there are lots of fun results from experiments that would best be measured via GC.
Those are more common in chemistry and biochemistry labs, and while I don't have direct access to one, I do have buddies that do. I also have a friend, (he works w/ proteins, I work w/ DNA), in the field who regularly does NMR, which I believe may be a step-up, but has a waitlist to use most weeks, and I don't really want to hassle him.
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r.lutece
gave Columbia her wings.


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Totally fair. My understanding is that the incubator would be awesome for colonizing petris (I assume that's its intended purpose?), as the mycelium on agar won't be generating much of its own heat. So, agar teks would be in your realm of possibility I imagine.
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Lab access [Re: r.lutece] 1
#23758929 - 10/21/16 07:21 PM (7 years, 3 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 11:59 AM)
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Inocuole
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Quote:
irequirechocolate said: simple things like a clean bench w/ a flame and a sharps container for syringe disposal, etc.
Why would you dispose of a perfectly good syringe? It wasn't stuck in a hepatitis patient.
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amidogen said: 86 F is far above an ideal temperature. Mycelial growth increases with temperature until topping out around 80F, but those temperatures are also much more hospitable to bacteria and mold. Ideal temps for both agar work, colonizing jars, and fruiting is 65-75F. I'm actually loving the fall weather because I'm not fighting at all to keep my place in the upper 60s. You'll see much fewer instances of contams in those temperature ranges. Mycelial growth will be slower, but your fruits will tend to be thicker and meatier.
All of this. I'm sure microbiologists have their own way of handling clean work but you might still want to just forget that and do it our way. 
http://www.shroomery.org/forums/showflat.php/Number/20048771#20048771 https://www.shroomery.org/forums/showflat.php/Number/22721954
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r.lutece
gave Columbia her wings.


Registered: 09/06/15
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Quote:
amidogen said: 86 F is far above an ideal temperature. Mycelial growth increases with temperature until topping out around 80F, but those temperatures are also much more hospitable to bacteria and mold. Ideal temps for both agar work, colonizing jars, and fruiting is 65-75F. I'm actually loving the fall weather because I'm not fighting at all to keep my place in the upper 60s. You'll see much fewer instances of contams in those temperature ranges. Mycelial growth will be slower, but your fruits will tend to be thicker and meatier.
I just double checked the post where I was getting that from and I realized that there was more discussion about it below. It was something RR quoted Stamets as saying, but apparently Stamets was quoting someone else and further testing disproved that. Mea culpa!
-------------------- One goes into an experiment knowing one might fail. But one does not undertake an experiment knowing one HAS failed.
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irequirechocolate
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Quote:
Inocuole said:
Quote:
irequirechocolate said: simple things like a clean bench w/ a flame and a sharps container for syringe disposal, etc.
Why would you dispose of a perfectly good syringe? It wasn't stuck in a hepatitis patient.
A couple reasons. There is no reason to risk contamination by airborne pathogens, especially when you have an unlimited supply as is - I have fresh ones available that I use daily. Plus I don't want to keep a bunch of needles/syringes around.
Quote:
amidogen said: 86 F is far above an ideal temperature.
Yes, I realize it's too high for my purposes. I primarily use it for bacteria & yeast . Still, the lab is kept at 68-72 F so those temps are not really a concern.
I will add that for me, I would really just use the lab for setting things up, not maintaining tubs. That's for home.
Edited by irequirechocolate (10/21/16 07:58 PM)
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Parafaragaramus
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I think if you have a sort of lab setting a good place to go is agar. I think the realistic progression usually goes pf cakes, grains, then agar. Some may go straight to grains. I've always wanted to dive into agar but the place I'm staying at now isn't the best for agar work so I have been sticking to grains and it has been just fine for me for a while now.
I think the transporting of things might get a bit tiresome. In the end they will still go to grains before being spawned in a tub or some other fruiting chamber. people have done great things at home with limited equipment. Are you planning on keeping the petris there until it's time to put them on grains?
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irequirechocolate
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Keeping plates in lab at RT is no problem. They're already all over the place as is. Admittedly I have not thoroughly read thru much of the agar threads yet. I glanced at some and saw people adding blue dye I think??? This would certainly stand out, as most plates in this lab are YEPD, a translucent yellow color, if not totally clear.
Above someone posted a link to some agar tek threads tho, so I'll check those out more seriously soon.
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Parafaragaramus
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I only have a limited knowledge of agar myself but I don't think I've read anything about a blue dye. as far as I know they're just that translucent yellow until the mycelium colonizes it and it turn a fairly stark white unless there is some sort of contamination.
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Leviticus969



Registered: 07/01/12
Posts: 939
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Congratulations on all the access to the lab gear but really imo, it isn't really necessary. Growing shrooms is easy. Growing extremely potent magical shrooms is the hard part.
Which leads me to this...
Quote:
So, my second question is, as an experienced cultivator, what type of questions would you like to see geneticists answer?
1. If you take a spore print from a parent shroom and grow from those spores will the mushrooms be identical in potency and appearance from the parent shroom or will it give you a reset on genetics and provide different results?
This is the single most important thing I want to know as an experienced cultivator. It's not even that hard to experiment on but I don't have the time, space, and energy for it right now.
So in other words, I'm trying to find out if Michael Jackson had babies what are the odds they'll end up exactly like their father.
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Inocuole
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Quote:
Archenemy_6 said: I only have a limited knowledge of agar myself but I don't think I've read anything about a blue dye.
 
Well where the hell have you been? It's food coloring btw. And it can be any color. It's only there to provide contrast.
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Leviticus969 said:
1. If you take a spore print from a parent shroom and grow from those spores will the mushrooms be identical in potency and appearance from the parent shroom or will it give you a reset on genetics and provide different results?
This is the single most important thing I want to know as an experienced cultivator. It's not even that hard to experiment on but I don't have the time, space, and energy for it right now.
You don't need a geneticist to tell you this. No, spore prints have thousands of genetics in them. Each mushroom can have multiple genetics within itself, and the spores don't just represent the mushroom you see. They're completely randomized and result in the possibility of thousands or even millions of individual strains from a single print. Even if a mushroom did only have one set of genetics in it (from an isolated strain), the spores would still be representative of more than just the mushroom you see.
The print could produce a good number of mushrooms that look like the parent, but it could also produce just as many mushrooms that are different.
Edited by Inocuole (10/21/16 09:10 PM)
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Parafaragaramus
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Quote:
Inocuole said:

Well where the hell have you been? It's food coloring btw. And it can be any color. It's only there to provide contrast.
I've definitely been living under a rock for a little while now. I've just recently started to try and get back into the swing of grains. But I can see how that can be useful.
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Leviticus969



Registered: 07/01/12
Posts: 939
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I don't have to be a geneticist to know this? Did I ask you? Mr Know it all? Have you studied mushrooms all your life? Who are you?
I'm not looking for the cliche status quo responses on here given by random growers that think they know everything when they just recycle outdated information on the shroomery so thanks and have a good day.
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Inocuole
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Quote:
Archenemy_6 said:
Quote:
Inocuole said:

Well where the hell have you been? It's food coloring btw. And it can be any color. It's only there to provide contrast.
I've definitely been living under a rock for a little while now. I've just recently started to try and get back into the swing of grains. But I can see how that can be useful.
Yeah dude I haven't seen you around in like, years.
Quote:
Leviticus969 said: I don't have to be a geneticist to know this? Did I ask you? Mr Know it all? Have you studied mushrooms all your life? Who are you?
I'm not looking for the cliche status quo responses on here given by random growers that think they know everything when they just recycle outdated information on the shroomery so thanks and have a good day.
Alright fucknut, ask the geneticist. Sorry I bothered correctly answering your stupid ass question.
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Leviticus969



Registered: 07/01/12
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Whatever man no one needs that condescending know it all attitude around here get a life.
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irequirechocolate
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Hopefully this will satisfy both of you: asexual spores are genetically identical to the parent. But, because of epigenetics and environmental cues, you're still not going to have an identical mushroom. EDIT: I have not reached the level of taking prints yet; I'm still waiting on my first set o'cakes. But maybe eventually I can extract DNA from a mother than daughter and sequence the two. This should not be hard to do.
Edited by irequirechocolate (10/21/16 09:22 PM)
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Inocuole
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Quote:
irequirechocolate said: Hopefully this will satisfy both of you: asexual spores are genetically identical to the parent. But, because of epigenetics and environmental cues, you're still not going to have an identical mushroom.
The spores aren't asexual though.
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SloppyJoseph
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Quote:
Leviticus969 said: Whatever man no one needs that condescending know it all attitude around here get a life. 
No what is not needed around here are jackass's who start shit when their questions get answered.
-------------------- AMU Q&A
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Leviticus969



Registered: 07/01/12
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I'd rather not have my questions answered by smart alecs that think I'm stupid
Quote:
You don't need a geneticist to tell you this.
Quote:
Alright fucknut, ask the geneticist. Sorry I bothered correctly answering your stupid ass question.

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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
irequirechocolate said: Hopefully this will satisfy both of you: asexual spores are genetically identical to the parent. But, because of epigenetics and environmental cues, you're still not going to have an identical mushroom. EDIT: I have not reached the level of taking prints yet; I'm still waiting on my first set o'cakes. But maybe eventually I can extract DNA from a mother than daughter and sequence the two. This should not be hard to do.
Awesome response. Thanks man and yes, it would be interesting to see the mother and daughter relation.
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Inocuole
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If the literal fact that you do not need a geneticist to tell you how mushroom spores work upset you, how do you propose you'll get anything done in life? And it was true, wasn't it? Because geneticist here is talking about asexual spores. Mushroom spores can have many sexes, and not all are inter-compatible. A single spore does not produce the mycelium we're looking for, therefore not identical to parent. They were right about epigenetics being a factor though. 
Frankly your insulting my effort to inform you, and calling me a know it all and being an ungrateful little prick should be considered a lot more offensive than my simply telling you that certain information is not necessarily geneticist level knowledge. Anyone who's successfully grown and participated in the community here would know these things. The point of being here is to talk about those things. If you just now waited for the opportunity to ask a geneticist, then that means you weren't using this site to its potential, which is the real shame here. If you'll ignore the correct answer in favor of the answer you want to hear a la appeal to authority, then I do indeed regret even bothering to share that information.
But you know, I actually do have a life, and I'm not interested in wasting it explaining myself to someone so thin skinned, so if you want to play the victim and act like you didn't walk right into everything I threw at you, then by all means.
Edited by Inocuole (10/21/16 09:49 PM)
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SloppyJoseph
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Quote:
Inocuole said: If the literal fact that you do not need a geneticist to tell you how mushroom spores work upset you, how do you propose you'll get anything done in life? And it was true, wasn't it? Because geneticist here is talking about asexual spores. Mushroom spores have many sexes, and not all are inter-compatible.
Frankly your insulting my effort to inform you, and calling me a know it all and being an ungrateful little prick should be considered a lot more offensive than my simply telling you that certain information is not necessarily geneticist level knowledge. Anyone who's successfully grown and participated in the community here would know these things. The point of being here is to talk about those things. If you just now waited for the opportunity to ask a geneticist, then that means you weren't using this site to its potential, which is the real shame here. If you'll ignore the correct answer in favor of the answer you want to hear a la appeal to authority, then I do indeed regret even bothering to share that information.
But you know, I actually do have a life, and I'm not interested in wasting it explaining myself to someone so thin skinned, so if you want to play the victim and act like you didn't walk right into everything I threw at you, then by all means.
And if that's tldr for you Leviticus...

And continue to start shit with respected cultivators, and take the advice of someone who's never taken a spore print on what spores are like. See how much longer you last around here.
-------------------- AMU Q&A
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irequirechocolate
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Quote:
Inocuole said:
Quote:
irequirechocolate said: Hopefully this will satisfy both of you: asexual spores are genetically identical to the parent. But, because of epigenetics and environmental cues, you're still not going to have an identical mushroom.
The spores aren't asexual though.
Despite my career, it's been a super long time since I took a bio class or anything that focused on the life cycle of fruiting fungi, so there are some major gaps in my knowledge. Still, the definition of a fungal spore is pretty much that: the asexual reproductive body of a fungus. This is actually kind of a heavy topic that I was going to start to get into, but instead just look at this. It's hella basic: http://bioweb.uwlax.edu/bio203/2011/bielmeie_luke/Reproduction.htm
Anyway, it's not really an issue. I'm just trying to work efficiently using what I have to get some nice and bulky results w/out having to buy spores over and over. (This is why I grow my own yeast for brewing as well).
After jumping around though, it looks like what I need to do is let my current cakes fruit, clone a fatty and then let it grow on some rich media agar plate. Unless you've got a protocol for this and/or how to move this over to a FC I will go back to mining old threads
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Pastywhyte
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Imperfect fungi are asexual. However we don't want to be growing those. Nasty. Cubes of course are higher fungi and not only do they require two spores to exchange genetic information they also need to be compatible with each other.
As far as potency and genetics go spores are essentially a reset. While some well stabilized lineages have tendencies, the true potential they have is (assuming no serious genetic degredation of the lineage) ultimately only limited by the genome of the species. As has been said many times, isolate and use agar to get consistent results. Anyone denying that is simply too lazy to put in the work and like all half ass cultivators will see much disappointment.
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irequirechocolate
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Found this on a super old thread, https://www.shroomery.org/forums/showflat.php/Number/15621268:
Quote:
mellowparty said: I inoculated some cube spores in YPD liquid media and left the flask on a shaker at 30degrees constant temperature. They germinated in a few days producing spherical colonies reminiscent of some LC contaminants (they produced mushrooms so no they were not contams)
I also inoculated some cube spores in YPD liquid and left them in a test tube at room temperature (around 20degrees). It took them longer to germinate and the mycelium they formed was not spherical colonies but irregularly shaped mass of mycelium. Then I used a vortex machine to break it apart and used that for mushrooms. Worked fine.
This would basically be routine practice for me in the lab. I'm going to try in out but I'm curious as to what might be the following steps once germination in liquid culture happens. Unfortunately the original poster was banned years ago...
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NDStepp84
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Lol @ putting spores in LC
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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irequirechocolate
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I may go in to lab over the weekend and set this up. We should know w/in a couple days. If I get growth I will use it to inoculate some jars at the same time as I use plain spores on others. Could be an interesting race.
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NDStepp84
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You don't need a lab to put dirty spores into sugar water, you can do that shit at home.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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SloppyJoseph
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Spore in LC is the worst way to germinate them. Use agar if you have a lab... or if you don't.
-------------------- AMU Q&A
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irequirechocolate
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Quote:
NDStepp84 said: You don't need a lab to put dirty spores into sugar water, you can do that shit at home.
Sure I could, but the lab has the added benefit of pre-made media, sterile flasks & tubes, incubators, shakers, roller drums, an autoclave for the jars, etc. We'll see wha happens.Quote:
SloppyJoseph said:
Spore in LC is the worst way to germinate them. Use agar if you have a lab... or if you don't.
I'll try it on some plates while I'm there.
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SloppyJoseph
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ND is trying to say it doesn't matter if your lab is sterile, the spores are dirty. At home you can make all the sterile media yourself too.
-------------------- AMU Q&A
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NDStepp84
Stellar nuclear waste


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What you don't get is no syringe or print is clean, spores are dirty it dosen't matter what you have if you inject contaminations into it, you can't look at an LC and tell if it's clean or not. That's what agar is for, you grow the culture on a 2d surface and transfer away from any contaminations. When done properly an LC is inoculated with a clean agar wedge, colonized then tested on agar. Edit
Check the date of the info on this site, use the advanced search feature to limit your search results to the last few years
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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irequirechocolate
Stranger

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Quote:
SloppyJoseph said: At home you can make all the sterile media yourself.
I don't doubt it, but in lab the media has already been made, saving me time & monies. There's cabinets full of liters and liters and stacks and stacks of plates.Quote:
NDStepp84 said: What you don't get is no syringe or print is clean, spores are dirty it dosen't matter what you have if you inject contaminations into it, you can't look at an LC and tell if it's clean or not. That's what agar is for, you grow the culture on a 2d surface and transfer away from any contaminations. When done properly an LC is inoculated with a clean agar wedge, colonized then tested on agar. Edit
Check the date of the info on this site, use the advanced search feature to limit your search results to the last few years
I gotchya. I can isolate from agar and move it to LC if needed. If the syringe comes w/ contaminated spores, why do people have such success just inoculating right into a cake jar w/out this being a concern?
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NDStepp84
Stellar nuclear waste


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BRF cakes are more forgiving compared to grain, and especially a low oxygen nutrient rich liquid solution. PF tek is a beginners method to get a few mushrooms, a cake contams no big deal throw it out, you have a bunch more. You have 5 quarts of grain spawned to bulk and one is bad you can loose everything.
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
Edited by NDStepp84 (10/21/16 11:42 PM)
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SloppyJoseph
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Use the shit out of those plates then. It would be awesome to have access to unlimited agar like that. Just don't waste your time making an LC with spores when you have all that.
-------------------- AMU Q&A
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irequirechocolate
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Quote:
NDStepp84 said: BRF cakes are more forgiving compared to grain. PF tek is a beginners method to get a few mushrooms, a cake contams no big deal throw it out, you have 5 quarts of grain spawned to bulk and one is bad you can loose everything.
Ah, I see. I haven't had any contam problems yet so I hadn't considered that the source would be from the syringe's contents. I assumed if someone had an issue it was due to improper sterilization of the jar and substrate.
Hey, while I've got your attention, what kind of grain do you use? I frequently home brew and have always felt like there must be a good way to re-use the malt grain after steeping it. It's still got a ton of nutritional content. I've had a buddy make bread w/ it before, but perhaps I could use it for this...
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NDStepp84
Stellar nuclear waste


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Most of us use whatever is cheapest/easily sourced with the hull still intact, whole oats, rye berries, wheat, wild bird seed preferably with no/low cracked corn.
--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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SloppyJoseph
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Malted barley would probably get too starchy to be useful for this. Nutritional content doesn't really matter, actually the richer it is the slower the mycelium will colonize.
-------------------- AMU Q&A
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irequirechocolate
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Quote:
SloppyJoseph said: Malted barley would probably get too starchy to be useful for this. Nutritional content doesn't really matter, actually the richer it is the slower the mycelium will colonize.
I see. I don't want to get too ahead of myself, but if I were to try using the spent stuff anyhow, maybe mixed w/ a less starchy grain, what in particular would you recommend reading up on? Some of you guys seem to have some of the better posts bookmarked and ready to share.
And btw thanks for the conversation. This is productive. As I try these things out I'll be sure to share results.
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Pastywhyte
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Spent grain has been grown on but yield suffers greatly with cubes, unless of course you have a killer culture. Try it with oysters.
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c10h12n2o
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a couple of things:
#1 dial back the animosity a little bit, or you will never learn anything. dont look for things to get upset about, try to understand what people are saying. you need to unlearn a LOT of incorrect assumptions, which is where we all start. the sooner you realize what the community has to offer and show a little humility/respect (especially when people are answering your questions ) the sooner you will be up to speed
#2 you need to be careful. whoever is paying for that lab is smart enough to know what you are doing, and it is a bad idea to build a tower of assumptions about what you think people know and care about. you sound like you are itching to get fired/arrested. please be careful, for your sake, and for the sake of our hobby
#3 most of the equipment you mention is not needed for these types of projects. things like a fume hood, sharps disposal, reagent suppliers, etc are completely unrelated to this kind of mycology. any experienced cultivator will have better stuff at their house (a flow hood/SAB, agar making supplies -- dirt cheap btw, people are so funny complaining about the cost of agar, scalpel blades and handles, pressure cooker, parafilm, culture tubes, etc). most of us in Mush-Cult have home labs much better equipped for and suited to magic mushrooms than the lab you are describing
#4 if you want to see some questions that would be awesome for a mycologist, or a geneticist who studies fungi, this is a thread i made on cubensis lifecycle questions. this is the type of stuff that experienced growers wonder about; what you are talking about is just misguided, and any amateur grower could explain why.
#5 i think you will need to learn a lot more of the basics before you will have a good idea of what constitutes a question that is worth bothering a professional with
#6 if you work in a DNA lab, it would be awesome to work out a process for distinguishing strains from one another genetically, mapping where the genetic differences are, or even just being able to determine if samples are genetically identical (ie, if something is REALLY a monoculture). there has been very little published work done on cubensis DNA. but again, you are just going to confuse yourself and anyone listening to you by trying to talk about this stuff before you figure out the basics
warm regards
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Quote:
Leviticus969 said: Whatever man no one needs that condescending know it all attitude around here get a life. 
Um.. I thought Inocuole gave you a pretty succinct and level headed answer. And the fact is, it isn't outdated information or incorrect either. He's right.
I think you're the one with an attitude. He didn't come in with an attitude but you sure responded with one.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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I have nothing new to add just want to be part of this thread. Congrats on your lab work. Everyone in here as attitude issues buts its ok. But lab workers are very judgemental because there pure agar cost 74 times what the food grade stuff does. Or reading a cultures gene scene while running a electrical current perfectly is the goal in a lab it isn't for normal people. The normal good people of the site are more caring about yeild there nothing with that. So just chill
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Lab access [Re: tump]
#23760027 - 10/22/16 05:30 AM (7 years, 3 months ago) |
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I never tried cubes on spent grain just edibles (and not even a lot, bran easier). Bw86 did a cool grow with them. It was sterile bags tho.
Need to use them ASAP and really watch moisture content of ur final mix cause those things are sticky icky
Don't talk down to the regulars, they help way more than you know. Not every teacher is the same...on here, in school, at jobs, etc. So man up and try to see the big picture of what this forum is about
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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mushroomnoob1981
Stranger


Registered: 06/15/15
Posts: 137
Last seen: 2 years, 7 months
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Is anyone in your lab doing mammalian cell culture? If so, they'll hate you if you bring anything fungus related in.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: tump] 1
#23760156 - 10/22/16 07:17 AM (7 years, 3 months ago) |
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Quote:
tump said: Everyone in here as attitude issues buts its ok.
Some of us even claim to have government grants to grow cubes..
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Quote:
Leviticus969 said: I don't have to be a geneticist to know this?
No, you don't. Ask Alan Rockefeller in the hunting forum if you doubt this; he's a geneticist and will tell you that you don't need him.
Quote:
Leviticus969 said:Did I ask you?
You asked the shroomery, a subset of which is Inocule. So yes, yes you did ask him.
Quote:
Leviticus969 said:Mr Know it all? Have you studied mushrooms all your life? Who are you?
1.) Mr. Knows more than you will do for now. 2.) It is only necessary to study mushrooms for a small part of your life to learn to answer YOUR questions. 3.) He's this guy on the Shroomery who knows more about mushrooms than you.
Quote:
Leviticus969 said:I'm not looking for the cliche status quo responses on here given by random growers that think they know everything when they just recycle outdated information on the shroomery so thanks and have a good day.
For this reason, I think I'll put you on ignore. I seldom put people on ignore. I mean, real assholes like people I don't think enough of to remember the names of right now aren't even on my list. But I'm going to skip the benefit of the doubt and assign you to that category of people who walk into a 20-year-old discussion and think that, with no knowledge of your own, you can just yell "Okay people LISTEN UP, now how the fuck do I do this?!?!" and assemble a roadmap to "super potent cubes" that no experienced cultivator before you has managed to glean from all their studies of this site and reality.
IOW, accept the help you asked for with a modicum of humility, or fuck off.
Best, Some random guy who regurgitates info from others
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Lab access [Re: Srirachi] 2
#23760312 - 10/22/16 09:08 AM (7 years, 3 months ago) |
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I can Only really get on here in the AM and u can tell when inoc isn't on by the amount of vague, foolish, and wrong answers in threads that aren't checked
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Sorry man, definitely been slackin, other shit picked up a bit. My posts per day graph is gonna take a hit for sure. It's been getting weird around here though, it's like even relatively normal people are catching the crazies and saying/doing dumb shit.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Lab access [Re: Srirachi] 1
#23760327 - 10/22/16 09:16 AM (7 years, 3 months ago) |
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:00 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: amidogen] 1
#23760347 - 10/22/16 09:28 AM (7 years, 3 months ago) |
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Quote:
amidogen said:
B) the only thing your lab really gives on the cult side is a good place to knock up agar plates or grain jars with your clean agar wedges...but you could do it at home for much less risk.
Gonna have to disagree. A good place to inoculate agar and grain is a place where you won't have to move it afterwards. Imagine having to drive your inoculated media back and forth from home and the lab. Insanely risky, in more ways than one.
OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about. Inevitably, it works out less than well for most. No matter what background you have, even if you've grown other mushrooms, to understand what we're doing here you have to forget everything you know, even if you had to pay money to know it. Yes that sucks when free information threatens to invalidate something you invested in, but large parts of life are just a joke in that way, unfortunately.
So to actually answer your question, I think the DNA sequencing and confirmation of certain features of isolates and clones, etc, like mentioned, would be a big help, but not if it means you're going to end up on the news. We're more interested in just having members who can grow mushrooms well and teach others the way than people who are willing to risk their career, their freedom, and our obscurity. I'm sure if you absorb the info here and then draw the connections from that to what you already know, after you've had some time to build up the fomer, at that time you'll be able to make some truly valuable contributions here.
I genuinely hope you make it that far. People like me can be offputting but as soon as you've been around the block for a bit you'll understand exactly where I'm coming from, and may even re-evaluate your take on my approach. I can tell you now I don't pay anyone off to respect any of my opinions.
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Leviticus969



Registered: 07/01/12
Posts: 939
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lol you guys can bitch about it all you want but at the end of the day it's like... just your opinion dude.
Quote:
Don't talk down to the regulars, they help way more than you know. Not every teacher is the same...on here, in school, at jobs, etc. So man up and try to see the big picture of what this forum is about
Just because I don't post on here regularly doesn't mean i'm some stupid newb that needs to be talked to in a condescending manner. Get over yourselves. And I certainly don't need a bunch of people with their "opinions" telling me I know nothing just because they don't even know me. That's pretty ignorant and arrogant. what a buncha chumps
I asked the OP, who happened to have a shit ton of genetic information piled up, a simple question for shits and giggles. It wasn't like it's some mad scientist golden information no one could ever find with the search engine.
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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You should have used the search engine then. I know, I know fuck me
-------------------- I would like to acquire anything I don’t have
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:00 PM)
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Leviticus969



Registered: 07/01/12
Posts: 939
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That's not the point. The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas. Nothing is ever set in stone and things can always be improved upon.
I asked the question for 2 reasons.... to first let the OP know about 1 of the things he can easily test for himself since he has access to all that lab gear. and 2, to let me, or anyone else on here, know the results of his experiments so that the whole community "might" learn something new.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:00 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Leviticus969 said: The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas.
This is pretty ironic considering we're talking about readily accepting wrong information while simultaneously denying correct information. Great! The system works! If you understood what appeal to authority meant you'd understand the logical fallacy in the very first interaction. Things just went downhill from there due to what is undoubtedly the result of a defensive and fragile ego.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Leviticus969 said: That's not the point. The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas. Nothing is ever set in stone and things can always be improved upon.
I asked the question for 2 reasons.... to first let the OP know about 1 of the things he can easily test for himself since he has access to all that lab gear. and 2, to let me, or anyone else on here, know the results of his experiments so that the whole community "might" learn something new.
So it has nothing to do with your mission to avoid growing "bad trip" mushrooms? Wasn't that always your bag? To find some variety that always grew "good trip" cubes instead of the "bad trip" ones.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Oh SHIT is this bad trip cube bonfire guy?? The dude who could stand to learn the most about genetics, basically?
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (06/21/18 11:30 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: amidogen] 3
#23760652 - 10/22/16 11:20 AM (7 years, 3 months ago) |
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Well this guy is 6Silent9Knight6 and that's why he's being shitty to me, because we've had run ins before. Hadn't noticed til now. Basically he believed that some mushrooms gave good trips and some gave bad trips, and he thought he could tell by looking at them, so he would allegedly burn piles of mushrooms that would have given bad trips. Naturally many laughs were had. https://www.shroomery.org/forums/showflat.php/Number/21897057#21897057
He's a charmer as you can see:
https://www.shroomery.org/forums/showflat.php/Number/21975841#21975841
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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I only knew because his title is still 'SPUN'
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Good catch.
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Thanks for all the info, guys. I think the reiteration of "agar agar agar" was good, if anything because it took me to reading a ton of stuff by SpitBallJedi and really has my gears turning, which 'feels good man.' I was actually dreaming about monotubs last night, no joke.
I hope no one on here thought I was carrying any animosity around, but if so, saaawryyy. Also, I aint trying to be like "I'm in science so I'm smarter." Not by a long stretch. Just introducing myself and setting up a background for the most part.
Aside from that, I'll be careful. Thanks for the concern, the accumulated knowledge, and the entertainment. Cheers.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Quote:
Inocuole said:
Quote:
amidogen said:
B) the only thing your lab really gives on the cult side is a good place to knock up agar plates or grain jars with your clean agar wedges...but you could do it at home for much less risk.
Gonna have to disagree. A good place to inoculate agar and grain is a place where you won't have to move it afterwards. Imagine having to drive your inoculated media back and forth from home and the lab. Insanely risky, in more ways than one.
OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about. Inevitably, it works out less than well for most. No matter what background you have, even if you've grown other mushrooms, to understand what we're doing here you have to forget everything you know, even if you had to pay money to know it. Yes that sucks when free information threatens to invalidate something you invested in, but large parts of life are just a joke in that way, unfortunately.
So to actually answer your question, I think the DNA sequencing and confirmation of certain features of isolates and clones, etc, like mentioned, would be a big help, but not if it means you're going to end up on the news. We're more interested in just having members who can grow mushrooms well and teach others the way than people who are willing to risk their career, their freedom, and our obscurity. I'm sure if you absorb the info here and then draw the connections from that to what you already know, after you've had some time to build up the fomer, at that time you'll be able to make some truly valuable contributions here.
I genuinely hope you make it that far. People like me can be offputting but as soon as you've been around the block for a bit you'll understand exactly where I'm coming from, and may even re-evaluate your take on my approach. I can tell you now I don't pay anyone off to respect any of my opinions. 
lol holy shit inoc, have you been writing campaign speeches or something? very succinct, polite, and comprehensive reply, extremely well put, you sound like a lawyer or something haha
even in the depths of my ignorance, a post like that would be enough to make me take a long hard look at my assumptions. nice job buddy, and spot-fucking-on

though i am only saying this becaus inoc pays me
LMFAO here is a quote from his thread:
Quote:
Leviticus969 said:
Quote:
lol... To answer your question... I burn all my grows in the fire pit in my back yard. I would not ever sell or give away bad shrooms with disgusting trips. I hate taking those trips myself and I would not want others to have those same trips. Then you get people on youtube posting their bad trips and disgracing the mushroom name
But yeah... I know for sure bad trips don't come from my head, set or setting. It's genetics.
If you look at the pictures of the potent strain i posted above... flush after flush after flush the potency was bomb as fuck and IDENTICAL. No matter where i ate them or how i was feeling. It was really out of this world magic.
he knows FOR SURE that set and setting have nothing to do with the way his brain interprets those weird shroomy neurotransmitter-like alkaloids: its definitely the genetics of the organism that produced them which modulates his experience.
LOL, such confidence. this is why the USA scores in the bottom of the 25 most developed nations for science, math, and language skills, but we score #1 on the question "how well do you think you did on the test?" funny but sad
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Idk that setting is most important for tripping. But i can tell a bad trip shrooms are too by looking at them if they aren't stored proper , black molded growing them always a bad time. Other then that i think its impossible to know.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Lab access [Re: tump] 2
#23761148 - 10/22/16 02:39 PM (7 years, 3 months ago) |
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Quote:
tump said: Idk that setting is most important for tripping. But i can tell a bad trip shrooms are too by looking at them if they aren't stored proper , black molded growing them always a bad time. Other then that i think its impossible to know.
Another invaluable insight brought to you by tump...
Seriously, how in the name of fuck do you get black mold on your stored shrooms? Better yet, how come you have the balls to eat something in that state? And you say bad trips? It's strange you don't end up having a trip to the emergency room one of these days.
How do you dry your mushrooms?
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
he knows FOR SURE that set and setting have nothing to do with the way his brain interprets those weird shroomy neurotransmitter-like alkaloids: its definitely the genetics of the organism that produced them which modulates his experience.
LOL, such confidence. this is why the USA scores in the bottom of the 25 most developed nations for science, math, and language skills, but we score #1 on the question "how well do you think you did on the test?" funny but sad
If shroom trips are based off of set and setting than we wouldn't be cloning for potency.
This is the equivalent to saying all weed strains are the same. They're not.... Some weed is literally bunk and some get you high as fuck.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:01 PM)
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AlCapone2k
Beginner


Registered: 10/06/16
Posts: 842
Loc: Germany
Last seen: 6 years, 4 months
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Common guys, all of you are respected members here with a lot of knowledge and it seems there was some misunderstanding, no reason to let the thread crash.
The intention of the OP was very noble, since he just wanted to "help" the community with information he thought he can gain because of his equipment. What we found out is, that his equipment is not really necessary for cubensis growth. Nonetheless it was well-intentioned.
Maybe there is some question he could answer with his knowledge, or maybe another one will answer it whith his knowledge. As long as the question is answered, everyone is happy I think
For example I'd like to hear his or one of the other guys opinion on the inoculation of agar plates. Better to put the loop or swab in the middle of the agar plate (growth from middle to the edges) or to get rid of bacteria better to drag it on with the known Z-form?
I tested both and as expected: The Z-form show mycelium everywhere, I think it will be quite hard to isolate from that plate. The middle-inoculation show a great mycelium growth from the middle heading towards the edges, isolation will be easier, and also no contamns til now (4 days sine inoc).
-------------------- Since I am from Germany, my english is not perfect but I try my best    My small Agar to Oyster Open Air Grow Trek
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
amidogen said: Ok. So following your reasoning it's all about the alkaloid content. You've got three possibilities: psilocybin, psilocin, baeocystin. My questions for you are:
A) what ratio of alkaloids do you think automatically gives you a good trip? Like if I eat shrooms with this alkaloid content and then go watch House on Haunted Hill with a bunch of cops, I'm still gonna be seeing rainbows and be in a blissful state of mind?
B) what the fuck sort of objective evidence do you have for this?
C) how exactly do you quantitatively (or even qualitatively) looking at some mushrooms you've grown and decide by eye the alkaloid content and then determine which you should burn?

A) Ratio's for the alkaloids for a good trip? Personally, i say fuck it to the ratios and scientific terms for potency. I've never been one to take the institutions status quo too seriously as they are prone to mistakes and unless I'm able to test it out for myself to see it's validity. Psilocybin, psilocin, baeocystin are terms used to gauge potency but here on the erowid site it says itself:
Quote:
Please note that these do not represent average values for widely sampled populations. Just because a mushroom species has some samples that show high or low concentrations does not mean that all mushrooms of that type have levels matching those in this chart.

You can eat a cube off a grow and it'll make you want to commit suicide and you can eat a different cube grow and feel like heaven on earth. How does one measure the Psilocybin, psilocin, baeocystin contents? I don't have lab gear so I don't know. The only way to test it is to eat it (and in my case "sometimes" looks can help since I've grown/eaten a shit ton of cubes and have been able to distinguish somewhat by appearance)
B) Where is my objective proof? Myself. And you're probably going: Well who the hell is this guy?? Well, considering this mycology field is an illegal activity for most places in the world where can you really get solid information other than random growers on the shroomery site... Like I said, nothings set in stone and there's still a LOT more to be known about the mushrooms. But here are a few quotes from RogerRabbit, a well renowned and respectable member of the community even though he's barely here anymore.
Quote:
Good luck. Science is never settled by popularity contests.
Potency is genetic, and that means that whatever genetics combine into the final mycelial network will determine the potency, provided you provide a medium on which it can grow and be healthy. After many years of growing on hundreds of different substrates, I can say from personal experience that there's not a bit of difference in potency when growing isolated strains on various substrates.
If one so-called 'strain' was always more potent, and one substrate was always producing fruits with more potency, don't you think we'd only be using those? RR
Quote:
Cubes are cubes. With multispore inoculation it's a crap shoot what you're going to get, just like with children. Saying that PE is always more potent is like saying black people are always better basketball players or that white people are always better golfers. Genetics fall where they might. Every family with ten kids has a genius, a retard, a good kid, a hoodlum, and the rest fall in the middle. It's the same way with 'strains'. Pick one and grow it. No cube is always more potent than any other. RR
And his information is not all correct also as I've had conflicting observations. But for the most part that's what the community is about... Chiming in our experiences and OPINIONS to come to a conclusion.
C) Quantatively/qualitively how do I determine which shrooms are bunk and which are bomb based off of looks?
Well that takes a shit ton of years of growing many different strains. But in my personal opinion, if you want something beautiful, rare, out of the ordinary magic kinda shit than first you'll have to "look" for what stands out before eating.
Here I'll throw in some pictures and let you decide which ones might be the potent boomers ( i just ripped this off of my potency thread so no cheating and if you already saw it than well....)
4 of these pictures are what I consider the mews of pokemon

So yeah to briefly sum it up looks definitely do help but the final most important step is to eat it. And quite frankly, I hate eating shrooms that make me want to commit suicide and believe me I've eaten quite a lot and endured a lot of those negative evil trips. It's a waste of my my life.
A truly euphoric magical strain would have you wanting to shroom out at least once a week. Not once every year.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Leviticus969



Registered: 07/01/12
Posts: 939
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Picture 1,4,5,7 all have high celler mass which means they have higher psilocybin. Do i win? I like pokemon
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: tump]
#23761957 - 10/22/16 07:16 PM (7 years, 3 months ago) |
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Shitposts... shitposts everywhere. Sorry OP.
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Quote:
Inocuole said: Shitposts... shitposts everywhere. Sorry OP. 
No worries. I might turn notifications off for a while though, hah
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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I mean to ask op what project is your lab working on? Also what are your spects on the auto clave you have?
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Re: Lab access [Re: tump]
#23762105 - 10/22/16 08:08 PM (7 years, 3 months ago) |
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tump said: I mean to ask op what project is your lab working on? Also what are your spects on the auto clave you have?
I'd rather not start calling more attention to my workplace by mentioning the specifics. The autoclaves are very versatile as to what their setting can be - times/temp/psi/etc. TBH I can't remember what I had it at exactly, but I used a default liquid cycle setting, which is one that prevents sterilized liquids from boiling. I figured I didn't want to mess w/ the moisture within the substrate by over cooking it. The whole process takes ~90min, and then I let the jars cool for 5hrs to prevent scorching the spores to hell during inoculation.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Leviticus969 said: This is the equivalent to saying all weed strains are the same. They're not.... Some weed is literally bunk and some get you high as fuck.
It is nothing like saying that. Weed is a plant. There's all sorts of differences that make what you said possibly the worst example you could've picked to support your statement.
It's a bit like saying that because cats can't fly, they must be penguins.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Lab access [Re: Inocuole] 1
#23762189 - 10/22/16 08:38 PM (7 years, 3 months ago) |
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@OP, did you take a look at my thread? i have a running list of questions im trying to figure out, i think you might be interested in it Cubensis Lifecycle Questions
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AlCapone2k said:
For example I'd like to hear his or one of the other guys opinion on the inoculation of agar plates. Better to put the loop or swab in the middle of the agar plate (growth from middle to the edges) or to get rid of bacteria better to drag it on with the known Z-form?
I tested both and as expected: The Z-form show mycelium everywhere, I think it will be quite hard to isolate from that plate. The middle-inoculation show a great mycelium growth from the middle heading towards the edges, isolation will be easier, and also no contamns til now (4 days sine inoc).
it depends on what you are trying to do. if you are trying to get isolates, or isolate clean cultures, this is what i recommend, it has saved me a ton of time since i figured out how to streak properly instead of the "z-form" i did before

@Leviticus696 / Silent Knight / whatever:
look we are trying to keep things constructive, im amazed at the level of patience people are showing in dealing with you 
idk if you are just ignorant and insanely arrogant or just dumb as fuck with a ridiculous level of confidence. Inoc put it quite well:
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Inocuole said: OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about.
its really funny how often this happens: some go on to become great growers, others spread nonsense for years, getting further from solid techniques with every post. inoc, space, and a few others basically work a full time job here correcting the nonsense that some noob lurker might follow, just because it was stated like the person knew what they were talking about
as far as your horribly illogical ideas about "trips," you sound like you have not even a basic grasp of psychology, biology, much less pharmacology... also, you clearly dont know the definition of "subjective" vs "objective" (since your objective proof is "Myself" , what a stupid thing to say). i really dont think you can have this conversation until you learn what those two words mean.
a "trip" is by definition a subjective experience. an amount of alkaloids present in a fruit is an objective figure. amidogen is giving you WAAAAAY too much credit by asking you what ratio it would take, as if you even understand or had even thought about the pharmacology and psychology involved in psychedelic dosage enough to even be considering things like alkaloid ratios impact on perceived potency
its stupid to speculate about things that are established facts. set and setting are what controls good vs bad trips, the only thing. genetics might determine alkaloid ratios, and by consequence perceived potency, but the only reason people have bad or good trips is set and setting (which includes their psychological state). this is why 2 people on the exact same shrooms (or even synthetic psilocybin), same dose, can have two VERY different experiences, good trips, bad trips, etc., because a trip is by definition a SUBJECTIVE experience
you arrogant idiot
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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I was asking about the autoclave because at the lab i work at they have a lab Companion ST-85G Autoclave. Its a beast most labs depending on there design. Have a must smaller one. If you have a big one like our but don't complete access to all equipment. I'd just sneak large amounts of grain jars in the autoclave with other items. As well as not knocking up the grain there but at home. Making the cultures there might be risky for you. Later I'll ask you on how you like to divide your cultures and how far the dision into cells you damage without killing them?
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Leviticus969



Registered: 07/01/12
Posts: 939
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c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
Quote:
Size has as much to do with moisture content and care than anything else, so it's not a good indicator of what to clone. In addition, with P cubensis, smaller fruits generally have more potency per gram of material than larger fruits.
You clone for the qualities you want in future flushes. Since clusters tend to produce more product in a given area, I prefer to clone a fruit from clusters. Of course there's other qualities that you also select for as well.
If growing a Psilocybe species, clone a fast growing, good pinning, potent fruit from a cluster. Since potency is largely subjective, sample a small part of your crop early, and if you like what you eat, clone a fruit from a large cluster the next morning. RR
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Leviticus969



Registered: 07/01/12
Posts: 939
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Here it is.... Quoted from the legend himself. Check yourself
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The biggest problem with multispore inoculation from syringes is the constant reports of zero potency. It's rare to actually get a total non-fruiting strain, but non-potent strains seem pretty common. A search here for low or no potency will return hundreds of threads on the subject.
When you isolate strains on agar, you grow each one to fruiting, carefully labeling each petri dish after you cut a wedge from it to inoculate grains. The petri dishes then go into the refrigerator for storage until you fruit each strain. When you do and determine the one superstrain that gives awesome yields and great potency, you go back to that dish, save it on a master culture slant, and grow it forever. The odds of getting a superstrain with syringes is very low and might take many years of trying. Then, when you actually get one of those, you realize you just ate them all and have nothing to clone with to keep going. This is why we use agar; you can do in a couple of months what it might take 20 years to find by accident using multispore inoculation. RR
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
RogerRabbit said:
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passenger said: am i cloning just the characteristics of the mushroom or am i cloning the potency as well? thanks.
What makes potency not a characteristic? RR
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:01 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Leviticus969 said: c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
You cannot be this dense. In case you are, it's because potency is not the same as chemicals that can have different effects from each other. Potency is a number. It scales.. linearly. Like fucking numbers god damn you. You can't drink beer until you feel the effects of LSD.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Reading gives you information, doing gives you knowledge. Sort of like the difference between reading the phone book and Walden.
RR probably also has some quotes out there that give what you posted context. There are no "super-potent cubes" but there are people fucked up enough that they can't even achieve normal potency. You don't clone weak shrooms is all RR says here. Cloning doesn't result in more potency over time; it leads to reduced potency.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Saying that psilocin psilocybin and the few other indoles that may cause psychoactive effects can be bad or good in some mushrooms and not others. Well it's by far the most fucking retarded thing ive seen posted here this month.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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doesnt even read the rules well enough to know he could get banned for triple posting, much less the basics of cultivation. just googles "site:shroomery.org potency genetic" , nevermind the people trying to explain the relationship between those two terms 
Quote:
Leviticus969 said: c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
you are still completely missing the point. yes, we clone for potency, among many other things. that is because there can be HUGE fluctuation in the levels of alkaloids between strains of cubensis.
genetics is only one part though, that only determines POTENTIAL. just like an NBA player's kid might have the potential to be great at basketball, in the right conditions he could also suck, and never reach that potential. A strain could be particularly potent (prone to high levels of alkaloids), but all that means is it has the potential to be potent, not that it will be potent. you are ignoring every single other part of the equation, and you dont even understand the genetics part
dosage of alkaloids certainly affects the intensity of the trip, and some fruits will have more alkaloids than others. but whether it is a "good trip" or "bad trip" is totally subjective, just like "good cheese" or "bad cheese"
i guarantee you, even with the mushrooms you think ensure a "good trip", if i were to chain you in a closet and abuse you every way i could think of for 3-4 months, then feed you some of those "good trip shrooms" and you would be miserable and terrified, the definition of bad set and setting, the definition of a bad trip
set and setting determines whether you will have a good or bad trip, nothing else. genetics AND environment/conditions determine the amount of alkaloids present, which is a broad indicator of perceived "potency"
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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So take his advice and isolate or clone for your consistent potency and good trip vibe mushrooms. Quit dicking around with cakes and ms if your taste is so refined.
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Leviticus969



Registered: 07/01/12
Posts: 939
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wow how about instead of asking me to provide proof, how about you provide proof and I can take a shit on it. It works both ways. You think my info is shit and I think yours is too. It's a win win situation.
if everyone were to jump off a bridge would you also follow? = sounds a lot like you guys.
The point I was trying to make is that there are A SHIT TON of zero magic, bunk shroom trip reports all over the psychedelic forum and youtube videos. This is probably cuz dumbasses grow shit and sell it to unaware people. Are you guys guilty of this or what? Is this why most of yall so stubborn to any different research and ideas? pssh must be. It's no wonder yall blame shit on set and setting when your shrooms are just bunk.
Edited by Leviticus969 (10/22/16 10:32 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:01 PM)
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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A bunk shroom isn't necessarily the growers fault. The potency of a shroom is like... Alcohol content. I guess cause I have to explain it to someone like you. A bunk shroom is like a can of beer. To achieve the results of let's say, half a fifth of tequila, you need to drink like 15 cans of beer. You will still get just as drunk though technically, because you had to consume the same amount of ethyl alcohol, but you just had to take a lot more to get there. But you will still be just as drunk.
And then have you ever had that 1 tequila drunk where you cried and or pissed yourself and or vommed and had a terrible night? Does that mean it was a bad batch of tequila?
 You just binged it irresponsibly and took it the wrong way, had a fight with your girlfriend, just had a shit night. But your friend who drank the same tequila was just fine.
-------------------- AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Leviticus969 said: wow how about instead of asking me to provide proof, how about you provide proof and I can take a shit on it. It works both ways. You think my info is shit and I think yours is too. It's a win win situation.
if everyone were to jump off a bridge would you also follow? = sounds a lot like you guys.
The point I was trying to make is that there are A SHIT TON of zero magic, bunk shroom trip reports all over the psychedelic forum and youtube videos. This is probably cuz dumbasses grow shit and sell it to unaware people. Are you guys guilty of this or what? Is this why most of yall so stubborn to any different research and ideas? pssh must be. It's no wonder yall blame shit on set and setting when your shrooms are just bunk.
Nobody here is a dealer and we are all well aware of how to avoid bunk fruits. If you would shut your hole and listen you could do it to. Them YouTube morons fail cause they do the same shit you are doing. I have grown bunk fruits. As soon as a culture came out bunk it hit the trash. When proven good it went in a slant. Then grow the good culture going forward. Grow like a noob get noob results. As long as your trying to grow "good trip shrooms" or "dealer grade" based on variety or looks, the possibility of fail will be strong.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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this thread is hilarious yeah, me and pasty and inoc grow shitty shrooms, who are we? lmao
this guy is just an idiot, plain and simple.
as i and many others have explained, a basic grasp of psychology and biology is all it takes to prove that set and setting determines whether a trip will be good or bad. we have repeatedly explained that there is a variation in the levels of potential potency between strains, and the way dosage impacts the experience. most of all, the fact that trips are a subjective experience.
if this guy was anywhere north of retarded he would realize what is wrong with thinking there are "good trip shrooms" and "bad trip shrooms"
like i said before:
Quote:
i guarantee you, even with the mushrooms you think ensure a "good trip", if i were to chain you in a closet and abuse you every way i could think of for 3-4 months, then feed you some of those "good trip shrooms" and you would be miserable and terrified, the definition of bad set and setting, the definition of a bad trip
im just glad inoc bothered mentioning this guy's infamous burn pile, this shit is the funniest/dumbest thread ive seen in a while, even beats the guy with the shed and handgun the other day lol
we love diverse thought, debate, intelligent discussion, and real experimentation here, check out my lifecycle questions thread if you want to see some of the stuff we think about. this is certainly not a homogeneous group of opinions in general, we have different opinions on everything from grain soaking, spawning at fruit, getting isolates, and a thousand other things. but it seems like we can all agree on what an idiot this guy is. if he was going connect the dots he would have at least quit talking shit by now
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Leviticus969



Registered: 07/01/12
Posts: 939
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lol i'm here all day. You guys are ass backwards as fuck. And yes, I am calling you out on your stupid shit. You guys grow bunk shit deal with it LOLOLOLOL
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SloppyJoseph said: A bunk shroom isn't necessarily the growers fault. The potency of a shroom is like... Alcohol content. I guess cause I have to explain it to someone like you. A bunk shroom is like a can of beer. To achieve the results of let's say, half a fifth of tequila, you need to drink like 15 cans of beer. You will still get just as drunk though technically, because you had to consume the same amount of ethyl alcohol, but you just had to take a lot more to get there. But you will still be just as drunk.
And then have you ever had that 1 tequila drunk where you cried and or pissed yourself and or vommed and had a terrible night? Does that mean it was a bad batch of tequila?
 You just binged it irresponsibly and took it the wrong way, had a fight with your girlfriend, just had a shit night. But your friend who drank the same tequila was just fine.
Ok so tequila is on the same level as cubes now? Bruh you're dumb as fuck. Where's your scientific evidence bruh? IS heroin on the same level as cubes also? durrrrrrrrrrhhhhh
Quote:
Nobody here is a dealer and we are all well aware of how to avoid bunk fruits. If you would shut your hole and listen you could do it to. Them YouTube morons fail cause they do the same shit you are doing. I have grown bunk fruits. As soon as a culture came out bunk it hit the trash. When proven good it went in a slant. Then grow the good culture going forward. Grow like a noob get noob results. As long as your trying to grow "good trip shrooms" or "dealer grade" based on variety or looks, the possibility of fail will be strong.
I don't think no one here is aware of how to avoid bunk fruits let alone be able to tell what bunk is. This whole thread proves it. And yeah, i'm sure all yall grow tubs upon tubs for personal use. Who the fuck do you guys think ya tryna fool.
It's better talking to a brick wall than talking to stubborn narrowminded idiots. Mush cult has become such a joke since RR and RK left. How pathetic.
No wonder why people leave this hobby after a grow or 2. Dealing with you hateful misinformed fucktards on here is sad. Just sad.
Edited by Leviticus969 (10/23/16 01:16 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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yeah, me, pasty, and inoc grow the bunkest shrooms around, we are trying to deal with it
check out some of my bunk ass shrooms from this month:
 
thats 1 flush from a 66qt tub, 3498 grams from 1 flush from 1 tub. 1 fruit weighed 295 grams. and the fuckin guy who is still doing cakes is gonna tell me whats bunk
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Leviticus969



Registered: 07/01/12
Posts: 939
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Those are nasty ass looking shrooms and i can tell they suck like shit just from looking at it. Look at all those nasty as holes in em. It's deformed like your brain. You act like I've never grown tubs or grown huge fruits just cuz I dont post all my grows. Wow you are desperate. Also, fyi mr smarty pants, it doesn't fucking matter what substrate you grow shrooms on. lmao but you believe so since that's what everybody else believes. If you want me to find the rr quote on that i'll be glad to.
And what's up with that dick slapping pic? Are you a raging homo or what? That's disgusting. Wow there are such degenerates on here.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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right right... we all grow shitty shrooms, gotcha 
because every post you make reminds me of the look on that girls face before she gets the dick in the face, it suits your thread
its kinda unbelievable that you are so dense. amidogen, pasty, inoc, and everyone else have been remarkably patient with you, and explained whats wrong with your thinking 10 different ways like they were tenured professors dealing with a donor's grandson, not experienced growers volunteering to help assholes for free
but i guess thats why god made cockslaps
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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ZZZZzzzzz Choose your own battles, d00ds. This got boring as fuck. Signed, OP
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Leviticus969



Registered: 07/01/12
Posts: 939
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I don't really care for close minded bigoted know it alls that actually don't know shit. It's remarkable you're still pressing on the idea that this thread has been golden information to me. You're backwards as fuck. Wake up from lala land.
PS. Are those really the best shrooms you've got to show off? They look horrible. What a joke. And you think you got useful info for me?
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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This guy doesn't know the difference between cubes and Ps. cyans: https://www.shroomery.org/forums/showflat.php/Number/23738564#23738564
I think that's reason enough to disregard anything he says.
Quote in case he edits it:
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Leviticus969 said:
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liloldme said:

 
What are those are those cubes 
Nice triple post on the last page too. It really is time to stop.
I'm not usually a shit disturber, but this thread is something special...
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: Lab access [Re: Greg]
#23763147 - 10/23/16 03:25 AM (7 years, 3 months ago) |
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Well excuse me for not being an encyclopedia for the zillions of different fungi out there. But we're talking about cubes here buddy. And quite frankly I don't see the correlation for cyans helping out in this thread about cubes. Another stupid ass sheep. Fuck you guys are retarded.
Keep em coming. I've got all day. (until one of these mods decide to ban me for speaking against the tide like some fucking nazis)
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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You don't have to be an encyclopedia to see the obvious differences between the two species. If you can tell how potent a cube is by looking at a picture, you should damn well be able to tell if it's even a cube!
I've never seen someone preemptively insult the mods for a ban, that's a new one.
Edited by Greg (10/23/16 03:40 AM)
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wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 10 hours, 4 minutes
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Quote:
Leviticus969 said: Mr. smarty pants, it doesn't fucking matter what substrate you grow shrooms on. lmao but you believe so since that's what everybody else believes.
It matters. The same genes used for mudafuka bottles will look different than ones that grew on straight oats for example.
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
Greg said: You don't have to be an encyclopedia to see the obvious differences between the two species. If you can tell how potent a cube is by looking at a picture, you should damn well be able to tell if it's even a cube!
I've never seen someone preemptively insult the mods for a ban, that's a new one.
Are you kidding me dude? This whole thread we've been talking about different genetic make up of various strains and aesthetics. This means that cubes can widely range in physique let alone potency.
And to clarify for your judgemental ass I thought it looked different that's why I asked to make sure if it was something different and if it was I wanted to know what it was. Wow sheep. Go back to sleep.
Quote:
wtfcrazymofo said:
Quote:
Leviticus969 said: Mr. smarty pants, it doesn't fucking matter what substrate you grow shrooms on. lmao but you believe so since that's what everybody else believes.
It matters. The same genes used for mudafuka bottles will look different than ones that grew on straight oats for example.
Quote:
RogerRabbit said:
Quote:
are aborts really more potent?
No.
Small mushrooms are more potent. Since aborts are small mushrooms, they're more potent for that reason. It's not because they're aborts. You can pick any mushroom at the pin size and get that extra potency. RR
Quote:
I'm afraid I'll have to agree with Ralphster and hippie3. I've tried all sorts of substrates, and all sorts of additives and have never found a magic bullet that I can add to a substrate that will make potent mushrooms every time.
I can make very pretty mushrooms(brf), and very large mushrooms(straw), and lots and lots of mushroooms(horse manure). Horse manure in my experience is one of the best bases to start with. Not due to any potency increase, but due to having almost the perfect texture and makeup. RR
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Good luck. Science is never settled by popularity contests.
Potency is genetic, and that means that whatever genetics combine into the final mycelial network will determine the potency, provided you provide a medium on which it can grow and be healthy. After many years of growing on hundreds of different substrates, I can say from personal experience that there's not a bit of difference in potency when growing isolated strains on various substrates.
If one so-called 'strain' was always more potent, and one substrate was always producing fruits with more potency, don't you think we'd only be using those?
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RogerRabbit said: Every noob goes through this. They don't seem to understand that every single multispore grow is going to have different potency. Therefore, one grow goes on pf cakes, the other goes on rye to manure. Whichever one is more potent, he naturally thinks it's because of the substrate. We've been going through this for years. RR
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RogerRabbit said:
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inoculatedGreif said: the potency depends on what you grow it on. to say, brf cakes are 10 less potent then ones grown on hpoo.
I don't think you can back that up. Lots of experienced growers still use brf cakes. Potency isn't as simple as choice of substrate. RR
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RogerRabbit said:
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. The entire tub was almost all mutants and blobs.
Aborts and small pins/mushrooms are considerably more potent by weight than large, mature fruits. In addition, psilocin degrades during the drying process so eating fresh mushrooms will always result in a stronger trip. RR
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Poo is what mommy changed out of your diapers and we don't grow mushrooms on it. We use manure, straw, coir, cotton waste, hay, chopped weeds, etc., as bulk substrates. However, none will provide increased potency over any other, since the choice of bulk substrate doesn't change the genetics. RR
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RogerRabbit said: <img src='http://www.shroomery.org/forums/images/moved.gif'> This thread was moved from Mushroom Cultivation.
Reason: This question gets asked at least once a week, and has been answered hundreds of times, even though it's off topic in cultivation. Every time it comes up, people without a clue take a wild guess, thus you find answers all over the board, usually wrong.
Smaller fruits are more potent per gram than larger fruits. That's a fact. Nobody can give specific figures because it differs from grow to grow due to the variances of multispore inoculation.
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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This guy's post history is a goldmine.
Not only does he not know what cyans look like, he apparently isn't so sure of himself with cubes either: https://www.shroomery.org/forums/showflat.php/Number/23707325 That thread was only 19 days ago.
Have another albino.
Edited by Greg (10/23/16 04:14 AM)
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Lab access [Re: Greg]
#23763194 - 10/23/16 04:09 AM (7 years, 3 months ago) |
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Wow this fucking guy is a jewel.. -He quotes RR non-stop, even though RR would kick his retarded ideas in a second. -His self-delusion is something out of this world. -He's not even funny. -Petty troll who does not even entertain. -Stinky noob who'll never know any better.
Dude, I normally feed the trolls because is something we need in mush cult once in a while to have some fun But in your case I only wish you'd shut the fuck up.
Please ban this bitch
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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jewel is right, more like a kidney stone
i love how he says rude and provocative things to the people who tried to explain stuff to him (still amazed at the lengths amidogen and inoc have gone to to try to make him connect the dots), and then starts cryin calling everyone " close minded bigoted know it alls that actually don't know shit," "sheep," and every other out-of-context insult he can think of when someone gives it back to him and actually makes sense
right right, we grow bunk shrooms, and you will get banned for "speaking your mind," definitely couldnt be because you completely ignore the forum rules about double/triple posting....
please, keep sending me PMs like this one telling me how stupid i am, it really makes you look smart:
Quote:
You're fucking retarded and the fact that you think anybody in their right mind would want to trip out on shrooms while being locked up in a closet is a poor example and really quite desperate.
Oh well, bomb ass shrooms aren't for everybody. For you it seems bunk garbage is your specialty. And no, you don't give a fuck about intelligent discussion because all you've been spewing is narrowminded hatred for anything that doesn't fit in with your puny little head comfort zone of yours
again, right over your head. if there are "good trip shrooms" then i could chain you in a closet and abuse you mentally/physically/sexually/spiritually and every other way i could think of for a few months, then feed you a massive dose of your non-bunk, "good trip shrooms" and youd have a splendid, lovely trip right? or would you be terrified and miserable, because of the set and setting: the definition of a bad trip.
you are a cry-bully idiot who doesnt even realize when he has been slapped in the face with a cock, rhetorically speaking
, because you deserve it, and all the insecure feelings it gives you
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Leviticus969



Registered: 07/01/12
Posts: 939
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Re: Lab access [Re: Josex]
#23763219 - 10/23/16 04:40 AM (7 years, 3 months ago) |
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Quote:
Josex said: Wow this fucking guy is a jewel.. -He quotes RR non-stop, even though RR would kick his retarded ideas in a second. -His self-delusion is something out of this world. -He's not even funny. -Petty troll who does not even entertain. -Stinky noob who'll never know any better.
Dude, I normally feed the trolls because is something we need in mush cult once in a while to have some fun But in your case I only wish you'd shut the fuck up.
Please ban this bitch
Yeah. you said it. not me. you guys are bored idiots that want to have fun with people by trolling them. I'll let you in on a secret.... I'd wish for you to shut the fuck up also. ooOOOooOOoooOOOo what now!
In case you haven't figured it out right by now.... I'll repeat myself for all you brain dead morons.... MUSH CULT INFO IS NOT PROVEN SCIENCE. There will be some things we agree on and others NOT SO MUCH.
Really guys, it's not that complicated. Do you guys do this in real life also? Go around and stomp your feet like lil bitches until you get your way? Are yall nazi commies that go around telling everyone your way is right and that you are perfect?
I don't understand that commie attitude. Yall talk like you can't EVER be wrong not even the slightest bit. Of course it sets you up for disappointment and failure when someone else comes along that tries to prove you wrong. I can see why yall so mad but seriously, grow up and learn to humble yourselves.
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
c10h12n2o said:
you are a cry-bully idiot who doesnt even realize when he has been slapped in the face with a cock, rhetorically speaking
, because you deserve it, and all the insecure feelings it gives you

Oh yeah virtual reality dick slaps really get me insecure!! You're a degenerate homo for even entertaining this idea.
And you're calling me the crybully idiot? In case you haven't noticed it's everyone in this thread versus me except one or 2 other guys. So you want to call me the bully? I shared my "opinion" with you guys in hopes of furthering the community and got nothing but negative ratings, curse words, and judgemental bs. And then you wanna call me the bully, the only guy versus everyone else, for cursing back.
Your desperation to win an argument is just sad bruh. Why do you even entertain me if I'm "wrong". And you wanna call me insecure? Get real bruh you aint foolin no one. Don't you got better things to do than prove your stance to someone with an opinion?
Quote:
Opinions are like assholes, everybody's got one
Geez what a bunch of hypocrites
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IAMNOTALLAMA
Sock Wearer


Registered: 09/28/16
Posts: 92
Last seen: 3 years, 6 months
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*Sigh* You wanna go to prison? Cause that's how you go to prison.
I also have been tempted to use lab resources but just don't do it.
Don't steal lab equipment; don't utilize lab resources for personal use.
-------------------- "It stands as one of the richer ironies of the drug war that the creation of a powerful new taboo against marijuana led directly to the creation of a powerful new plant" - The Botany of Desire
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Quote:
Inocuole said: Well this guy is 6Silent9Knight6 and that's why he's being shitty to me, because we've had run ins before. Hadn't noticed til now. Basically he believed that some mushrooms gave good trips and some gave bad trips, and he thought he could tell by looking at them, so he would allegedly burn piles of mushrooms that would have given bad trips. Naturally many laughs were had. https://www.shroomery.org/forums/showflat.php/Number/21897057#21897057
He's a charmer as you can see:
https://www.shroomery.org/forums/showflat.php/Number/21975841#21975841
OMG it's this guy!? The dunce that can rate what level of trip a shroom will give by looking at it!! But, at the same time cannot ID the cubes they grew or Cyans.
Man, I haven't seen someone fail so hard in a while. Thanks for the LuLz!
Stay in school kids!!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: Juiceh]
#23763557 - 10/23/16 08:43 AM (7 years, 3 months ago) |
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Quit trying to regress the community here back into your stone age ponderings and just leave, seriously. You're in a thread occupied by mods and TCs calling people degenerate homos. You know, I think that's bannable. I sure hope they do.

Would sure be nice if OPs thread didn't get completely shat on by your appearance. I'd say lock it, but they don't deserve that.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:02 PM)
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Re: Lab access [Re: amidogen] 2
#23763598 - 10/23/16 09:11 AM (7 years, 3 months ago) |
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When people quote RR nonstop, it is usually a sign that they have zero actual experience.
I don't say this as any sort of comment on RR's knowledge or contributions, but rather I compare their thought patterns to those of cave people in sci fi movies, who build a religion around some completely misconstrued artifact from a more advanced culture.
Their endless copy-paste posts make me grin, as I imagine them emerging from their cave into their certain destruction, all the while holding up the laserdisc from the Voyager probe above their heads as if it makes them invincible, completely unaware that they are about to be squished.
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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Sorry OP, your thread got invaded by a ...... I wish there was an image of shroom cluster that looked like a hand giving the middle finger.....bad trips to be had.
-------------------- I would like to acquire anything I don’t have
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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Quote:
Leviticus969 said: lol i'm here all day. You guys are ass backwards as fuck. And yes, I am calling you out on your stupid shit. You guys grow bunk shit deal with it LOLOLOLOL
Quote:
SloppyJoseph said: A bunk shroom isn't necessarily the growers fault. The potency of a shroom is like... Alcohol content. I guess cause I have to explain it to someone like you. A bunk shroom is like a can of beer. To achieve the results of let's say, half a fifth of tequila, you need to drink like 15 cans of beer. You will still get just as drunk though technically, because you had to consume the same amount of ethyl alcohol, but you just had to take a lot more to get there. But you will still be just as drunk.
And then have you ever had that 1 tequila drunk where you cried and or pissed yourself and or vommed and had a terrible night? Does that mean it was a bad batch of tequila?
 You just binged it irresponsibly and took it the wrong way, had a fight with your girlfriend, just had a shit night. But your friend who drank the same tequila was just fine.
Ok so tequila is on the same level as cubes now? Bruh you're dumb as fuck. Where's your scientific evidence bruh? IS heroin on the same level as cubes also? durrrrrrrrrrhhhhh
You're a fucking idiot. It's called an analogy. We all learned what that was in 3rd grade. I tried to put it in lame terms so you could wrap your tiny stubborn brain around it, but that proves too difficult as well. GTFO troll. I'm waiting for you to slip up so Pasty can ban your ass because you are turning this into a fucking circus.
-------------------- AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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I never needed to ban him, it's already been done. Besides I'm not a mush cult mod anyways.
To be honest when I left this disaster of a thread I still had some hope for him. But now I see he was really just trolling. No one is that obtuse. Just let it die.
A sad way to ruin the OP's thread. At any rate I'm sue he learned a thing or two so I guess it's not a total loss. Most of his lab access is not really super useful but if he ever gets a look at that spectrometer hopefully he has some isolates to use as samples. He best get growing like a boss so the real questions about potency can be answered.
I wonder if these look potent, probably bad trip shrooms tho. . .
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PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
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-------------------- I would like to acquire anything I don’t have
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Pasty can i have them. They loook so pretty. I can't wait to see op penis envy grows
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Re: Lab access [Re: tump]
#23771101 - 10/25/16 07:07 PM (7 years, 3 months ago) |
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BTW, the plates I inoculated w/ spores from a syringe on Sat are showing signs of growth. As an experiment I put two plates in a 30C incubator and have two at RT. Based on growth, 30C seems to be greatly preferred.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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It's not that the higher temps won't be appreciated by the fungi (to a point) they grow in places like india and Brazil after all. The problem is that the contams prefer those temps as well. The cubes however can handle low 70's just fine but baccilus and trich will be greatly slowed in those temps.
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Growing them at the higher temps seems like a good idea to me then, as if it's contaminated you can spot what's inevitably there faster. Then you can just transplant what you want to a fresh plate in order to isolate sooner.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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There is always going to be contamination. If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Inocuole said: There is always going to be contamination.
This. We colonize a fruit in a window. Sooner this is understood the sooner you will see consistent success. Best way to see fast colonization is via smart expansion methods.
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Quote:
bodhisatta said: Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
I'd wager the #1 reason is that they don't have a 30C incubator to even try out.
Quote:
Inocuole said: There is always going to be contamination. If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.
Yup. 100% willing to.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
irequirechocolate said:
Quote:
bodhisatta said: Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
I'd wager the #1 reason is that they don't have a 30C incubator to even try out.
There are dozens of teks on how to build an incubator right on these very boards. There is a reason they don't get bumped much anymore. But you will do what you want to do despite our advice.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Next
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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It's easy as fuck to build a Tub in a tub incubator for 15-20 bucks
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Its even easy to bluid a heat bomb and surrounded it by jars in a tote. The good old tit wasted a lot of enegry. With the same enegry used i run a small heater in my green house that holds 1356 qt jars at a solid 71f .
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Lab access [Re: tump] 1
#23771535 - 10/25/16 09:18 PM (7 years, 3 months ago) |
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1356 quart jars.
That's a lot of mold tump
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Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
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I was going to make a snarky as fuck comment about government work in reply to tump, but I withheld it. I live vicariously through you Bod.
Seriously though any noobs reading this, please don't use incubators unless you're in to sadomasochism.
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Yea not all of it is cubes. Edibles grow well in that temp too spawn run anyway. Ask pasty it gets out of hand if you lets it.
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irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
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Re: Lab access [Re: tump]
#23771552 - 10/25/16 09:25 PM (7 years, 3 months ago) |
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 Clearly not for personal use
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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I don't care if I get banned some people are retarded. And I'll call it
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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why jars in a greenhouse? obviously a fruiting chamber is good for fruiting, but spawn run??
and i dont think anyone has ever done half that many jars without considering graduating to bags
@OP, again, you are making tons of assumptions. Inoc's point rings just as true now as when he said it:
Quote:
OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about. Inevitably, it works out less than well for most.
i think you would probably be pretty amazed by the home labs some of us have, and some even work in labs similar to what you describe (some even more suited to mycology, like people in the brewing industry). its plain dumb to assume that people simply cant get access to an incubator, or any of the other out-of-context equipment/resources youve mentioned, thats just naive.
try to be less naive and more humble, you could learn something, these people know what they are talking about (most of them at least... )
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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Green houses because they are designed to hold heat and gas are perfect for temp control. Most of jars go in there, all my spawn runs plus fruiting happens in my basement where its very cold all year around. As for bags they are great for two time use max so not worth it most of the time. Like i said most are edibles strains so i have to protect the jars form the heavy oyster spore load.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
irequirechocolate said:
 Clearly not for personal use
Maybe that other locked thread didn't drive the point home, stop fucking talking about any use other than personal.
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mushroomnoob1981
Stranger


Registered: 06/15/15
Posts: 137
Last seen: 2 years, 7 months
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I said it once and I'll say it again– DO NOT BRING MUSHROOMS INTO THE LAB.
Fungi are a MAJOR contaminant do anything that involves agar or nutrient broth.
It doesn't matter if your lab doesn't use these things. In grad school, a guy contaminated the tissue culture room from halfway across the building.
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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This thread is sad. It seems op had a lot to bring to the table, but you guys all thought it would be more fun to just fuck with other people. You ALL need to check yourselves. If I could negative rate everyone in this thread with the click of one button, I would. But I can't so I'm not going to waste my time.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Lab access [Re: azur]
#23803438 - 11/05/16 01:39 PM (7 years, 2 months ago) |
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this post had it all! butthurts and rantings.
that dood called inoc and the others 'commie nazi' like how is that possible. opposite political ideologies!!
and tumps 1300+ jars going.. not to mention he did 116g of dried pan cyan(other thread)..
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Lab access [Re: azur] 1
#23803443 - 11/05/16 01:41 PM (7 years, 2 months ago) |
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Quote:
azur said: This thread is sad. It seems op had a lot to bring to the table, but you guys all thought it would be more fun to just fuck with other people. You ALL need to check yourselves. If I could negative rate everyone in this thread with the click of one button, I would. But I can't so I'm not going to waste my time.
Advocate the devil some more azur..
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Lab access [Re: azur]
#23803470 - 11/05/16 01:52 PM (7 years, 2 months ago) |
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Quote:
azur said: This thread is sad. It seems op had a lot to bring to the table
Sorry I missed that part. Care to enlighten me please. I thought that he wouldn't be able to use the GS.
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Lab access [Re: azur]
#23807279 - 11/06/16 04:20 PM (7 years, 2 months ago) |
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It's too bad leviticus got banned I wanted to know how this dude would rate PE blobs by looking at them or sclerotia?
Quote:
azur said: This thread is sad. It seems op had a lot to bring to the table, but you guys all thought it would be more fun to just fuck with other people. You ALL need to check yourselves. If I could negative rate everyone in this thread with the click of one button, I would. But I can't so I'm not going to waste my time.
Most of the lab equipment op mentioned really wasn't applicable. Fume hood & sharps disposal container?? Sweet... Plus, him risking doing illicit mycology in a school lab is just a really bad idea. I'm sure more professors than you realize would know exactly what he was doing very quickly. There was potential for an interesting genetics discussion though.
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