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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: SloppyJoseph] * 1
    #23763813 - 10/23/16 10:29 AM (7 years, 3 months ago)

I never needed to ban him, it's already been done. Besides I'm not a mush cult mod anyways.

To be honest when I left this disaster of a thread I still had some hope for him. But now I see he was really just trolling. No one is that obtuse. Just let it die.

A sad way to ruin the OP's thread. At any rate I'm sue he learned a thing or two so I guess it's not a total loss. Most of his lab access is not really super useful but if he ever gets a look at that spectrometer hopefully he has some isolates to use as samples. He best get growing like a boss so the real questions about potency can be answered.

I wonder if these look potent, probably bad trip shrooms tho. . .



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OfflinePortabellaFella 1
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Re: Lab access [Re: Pastywhyte]
    #23763903 - 10/23/16 10:54 AM (7 years, 3 months ago)

:ohboycomputer:


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Offlinetump
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Re: Lab access [Re: PortabellaFella 1]
    #23764178 - 10/23/16 12:39 PM (7 years, 3 months ago)

Pasty can i have them. They loook so pretty. I can't wait to see op penis envy grows


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Invisibleirequirechocolate
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Re: Lab access [Re: tump]
    #23771101 - 10/25/16 07:07 PM (7 years, 3 months ago)

BTW, the plates I inoculated w/ spores from a syringe on Sat are showing signs of growth. As an experiment I put two plates in a 30C incubator and have two at RT. Based on growth, 30C seems to be greatly preferred.


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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: irequirechocolate] * 1
    #23771120 - 10/25/16 07:12 PM (7 years, 3 months ago)

It's not that the higher temps won't be appreciated by the fungi (to a point) they grow in places like india and Brazil after all. The problem is that the contams prefer those temps as well. The cubes however can handle low 70's just fine but baccilus and trich will be greatly slowed in those temps.


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Invisibleirequirechocolate
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Re: Lab access [Re: Pastywhyte]
    #23771223 - 10/25/16 07:40 PM (7 years, 3 months ago)

Growing them at the higher temps seems like a good idea to me then, as if it's contaminated you can spot what's inevitably there faster. Then you can just transplant what you want to a fresh plate in order to isolate sooner.


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InvisiblebodhisattaMDiscordReddit
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Re: Lab access [Re: irequirechocolate]
    #23771230 - 10/25/16 07:42 PM (7 years, 3 months ago)

Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine


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InvisibleInocuole
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Re: Lab access [Re: bodhisatta] * 1
    #23771248 - 10/25/16 07:48 PM (7 years, 3 months ago)

There is always going to be contamination.  If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.


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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: Inocuole]
    #23771266 - 10/25/16 07:56 PM (7 years, 3 months ago)

Quote:

Inocuole said:
There is always going to be contamination.




This. We colonize a fruit in a window. Sooner this is understood the sooner you will see consistent success. Best way to see fast colonization is via smart expansion methods.


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Invisibleirequirechocolate
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Re: Lab access [Re: Inocuole]
    #23771270 - 10/25/16 07:56 PM (7 years, 3 months ago)

Quote:

bodhisatta said:
Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine



I'd wager the #1 reason is that they don't have a 30C incubator to even try out.

Quote:

Inocuole said:
There is always going to be contamination.  If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.



Yup. 100% willing to.


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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: irequirechocolate]
    #23771285 - 10/25/16 08:01 PM (7 years, 3 months ago)

Quote:

irequirechocolate said:
Quote:

bodhisatta said:
Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine



I'd wager the #1 reason is that they don't have a 30C incubator to even try out.




There are dozens of teks on how to build an incubator right on these very boards. There is a reason they don't get bumped much anymore. But you will do what you want to do despite our advice.


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InvisibleInocuole
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Re: Lab access [Re: Pastywhyte]
    #23771308 - 10/25/16 08:10 PM (7 years, 3 months ago)

Next


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisiblebodhisattaMDiscordReddit
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Re: Lab access [Re: Inocuole]
    #23771340 - 10/25/16 08:18 PM (7 years, 3 months ago)

It's easy as fuck to build a Tub in a tub incubator for 15-20 bucks


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Offlinetump
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Re: Lab access [Re: Inocuole]
    #23771513 - 10/25/16 09:11 PM (7 years, 3 months ago)

Its even easy to bluid a heat bomb and surrounded it by jars in a tote. The good old tit wasted a lot of enegry. With the same enegry used i run a small heater in my green house that holds 1356 qt jars at a solid 71f .


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InvisiblebodhisattaMDiscordReddit
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Re: Lab access [Re: tump] * 1
    #23771535 - 10/25/16 09:18 PM (7 years, 3 months ago)

1356 quart jars.

That's a lot of mold tump


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OfflineGreg
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Re: Lab access [Re: bodhisatta]
    #23771540 - 10/25/16 09:21 PM (7 years, 3 months ago)

I was going to make a snarky as fuck comment about government work in reply to tump, but I withheld it.
I live vicariously through you Bod.

Seriously though any noobs reading this, please don't use incubators unless you're in to sadomasochism.


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Offlinetump
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Re: Lab access [Re: bodhisatta]
    #23771545 - 10/25/16 09:23 PM (7 years, 3 months ago)

Yea not all of it is cubes. Edibles grow well in that temp too spawn run anyway. Ask pasty it gets out of hand if you lets it.


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Invisibleirequirechocolate
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Re: Lab access [Re: tump]
    #23771552 - 10/25/16 09:25 PM (7 years, 3 months ago)


Clearly not for personal use


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InvisiblebodhisattaMDiscordReddit
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Re: Lab access [Re: irequirechocolate]
    #23771575 - 10/25/16 09:39 PM (7 years, 3 months ago)

I don't care if I get banned some people are retarded. And I'll call it


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Invisiblec10h12n2o
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Re: Lab access [Re: irequirechocolate]
    #23771579 - 10/25/16 09:41 PM (7 years, 3 months ago)

why jars in a greenhouse? obviously a fruiting chamber is good for fruiting, but spawn run??

and i dont think anyone has ever done half that many jars without considering graduating to bags

@OP, again, you are making tons of assumptions. Inoc's point rings just as true now as when he said it:

Quote:

OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about.  Inevitably, it works out less than well for most.




i think you would probably be pretty amazed by the home labs some of us have, and some even work in labs similar to what you describe (some even more suited to mycology, like people in the brewing industry). its plain dumb to assume that people simply cant get access to an incubator, or any of the other out-of-context equipment/resources youve mentioned, thats just naive.

try to be less naive and more humble, you could learn something, these people know what they are talking about (most of them at least...:rolleyes:)


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Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures


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