|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
I never needed to ban him, it's already been done. Besides I'm not a mush cult mod anyways.
To be honest when I left this disaster of a thread I still had some hope for him. But now I see he was really just trolling. No one is that obtuse. Just let it die.
A sad way to ruin the OP's thread. At any rate I'm sue he learned a thing or two so I guess it's not a total loss. Most of his lab access is not really super useful but if he ever gets a look at that spectrometer hopefully he has some isolates to use as samples. He best get growing like a boss so the real questions about potency can be answered.
I wonder if these look potent, probably bad trip shrooms tho. . .
|
PortabellaFella 1
Enthusiastic



Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
|
|
-------------------- I would like to acquire anything I don’t have
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
|
Pasty can i have them. They loook so pretty. I can't wait to see op penis envy grows
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
Re: Lab access [Re: tump]
#23771101 - 10/25/16 07:07 PM (7 years, 3 months ago) |
|
|
BTW, the plates I inoculated w/ spores from a syringe on Sat are showing signs of growth. As an experiment I put two plates in a 30C incubator and have two at RT. Based on growth, 30C seems to be greatly preferred.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
It's not that the higher temps won't be appreciated by the fungi (to a point) they grow in places like india and Brazil after all. The problem is that the contams prefer those temps as well. The cubes however can handle low 70's just fine but baccilus and trich will be greatly slowed in those temps.
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
|
Growing them at the higher temps seems like a good idea to me then, as if it's contaminated you can spot what's inevitably there faster. Then you can just transplant what you want to a fresh plate in order to isolate sooner.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
There is always going to be contamination. If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
Quote:
Inocuole said: There is always going to be contamination.
This. We colonize a fruit in a window. Sooner this is understood the sooner you will see consistent success. Best way to see fast colonization is via smart expansion methods.
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
|
Quote:
bodhisatta said: Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
I'd wager the #1 reason is that they don't have a 30C incubator to even try out.
Quote:
Inocuole said: There is always going to be contamination. If you're going to willfully give them an edge because you think your technique will compensate, well... best of luck.
Yup. 100% willing to.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
|
Quote:
irequirechocolate said:
Quote:
bodhisatta said: Do what you will but there's a reason everyone worth their salt uses room temperature 20-22C or so with 18-22C being fine
I'd wager the #1 reason is that they don't have a 30C incubator to even try out.
There are dozens of teks on how to build an incubator right on these very boards. There is a reason they don't get bumped much anymore. But you will do what you want to do despite our advice.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Next
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
It's easy as fuck to build a Tub in a tub incubator for 15-20 bucks
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
|
Its even easy to bluid a heat bomb and surrounded it by jars in a tote. The good old tit wasted a lot of enegry. With the same enegry used i run a small heater in my green house that holds 1356 qt jars at a solid 71f .
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: Lab access [Re: tump] 1
#23771535 - 10/25/16 09:18 PM (7 years, 3 months ago) |
|
|
1356 quart jars.
That's a lot of mold tump
|
Greg
always learning




Registered: 10/28/15
Posts: 1,536
Loc: an autoclave
Last seen: 3 months, 3 days
|
|
I was going to make a snarky as fuck comment about government work in reply to tump, but I withheld it. I live vicariously through you Bod.
Seriously though any noobs reading this, please don't use incubators unless you're in to sadomasochism.
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
|
Yea not all of it is cubes. Edibles grow well in that temp too spawn run anyway. Ask pasty it gets out of hand if you lets it.
|
irequirechocolate
Stranger

Registered: 10/15/16
Posts: 132
|
Re: Lab access [Re: tump]
#23771552 - 10/25/16 09:25 PM (7 years, 3 months ago) |
|
|
 Clearly not for personal use
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
|
I don't care if I get banned some people are retarded. And I'll call it
|
c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
|
|
why jars in a greenhouse? obviously a fruiting chamber is good for fruiting, but spawn run??
and i dont think anyone has ever done half that many jars without considering graduating to bags
@OP, again, you are making tons of assumptions. Inoc's point rings just as true now as when he said it:
Quote:
OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about. Inevitably, it works out less than well for most.
i think you would probably be pretty amazed by the home labs some of us have, and some even work in labs similar to what you describe (some even more suited to mycology, like people in the brewing industry). its plain dumb to assume that people simply cant get access to an incubator, or any of the other out-of-context equipment/resources youve mentioned, thats just naive.
try to be less naive and more humble, you could learn something, these people know what they are talking about (most of them at least... )
--------------------
  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
|
|