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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Leviticus969 said: This is the equivalent to saying all weed strains are the same. They're not.... Some weed is literally bunk and some get you high as fuck.
It is nothing like saying that. Weed is a plant. There's all sorts of differences that make what you said possibly the worst example you could've picked to support your statement.
It's a bit like saying that because cats can't fly, they must be penguins.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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Re: Lab access [Re: Inocuole] 1
#23762189 - 10/22/16 08:38 PM (7 years, 3 months ago) |
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@OP, did you take a look at my thread? i have a running list of questions im trying to figure out, i think you might be interested in it Cubensis Lifecycle Questions
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AlCapone2k said:
For example I'd like to hear his or one of the other guys opinion on the inoculation of agar plates. Better to put the loop or swab in the middle of the agar plate (growth from middle to the edges) or to get rid of bacteria better to drag it on with the known Z-form?
I tested both and as expected: The Z-form show mycelium everywhere, I think it will be quite hard to isolate from that plate. The middle-inoculation show a great mycelium growth from the middle heading towards the edges, isolation will be easier, and also no contamns til now (4 days sine inoc).
it depends on what you are trying to do. if you are trying to get isolates, or isolate clean cultures, this is what i recommend, it has saved me a ton of time since i figured out how to streak properly instead of the "z-form" i did before

@Leviticus696 / Silent Knight / whatever:
look we are trying to keep things constructive, im amazed at the level of patience people are showing in dealing with you 
idk if you are just ignorant and insanely arrogant or just dumb as fuck with a ridiculous level of confidence. Inoc put it quite well:
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Inocuole said: OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about.
its really funny how often this happens: some go on to become great growers, others spread nonsense for years, getting further from solid techniques with every post. inoc, space, and a few others basically work a full time job here correcting the nonsense that some noob lurker might follow, just because it was stated like the person knew what they were talking about
as far as your horribly illogical ideas about "trips," you sound like you have not even a basic grasp of psychology, biology, much less pharmacology... also, you clearly dont know the definition of "subjective" vs "objective" (since your objective proof is "Myself" , what a stupid thing to say). i really dont think you can have this conversation until you learn what those two words mean.
a "trip" is by definition a subjective experience. an amount of alkaloids present in a fruit is an objective figure. amidogen is giving you WAAAAAY too much credit by asking you what ratio it would take, as if you even understand or had even thought about the pharmacology and psychology involved in psychedelic dosage enough to even be considering things like alkaloid ratios impact on perceived potency
its stupid to speculate about things that are established facts. set and setting are what controls good vs bad trips, the only thing. genetics might determine alkaloid ratios, and by consequence perceived potency, but the only reason people have bad or good trips is set and setting (which includes their psychological state). this is why 2 people on the exact same shrooms (or even synthetic psilocybin), same dose, can have two VERY different experiences, good trips, bad trips, etc., because a trip is by definition a SUBJECTIVE experience
you arrogant idiot
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
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I was asking about the autoclave because at the lab i work at they have a lab Companion ST-85G Autoclave. Its a beast most labs depending on there design. Have a must smaller one. If you have a big one like our but don't complete access to all equipment. I'd just sneak large amounts of grain jars in the autoclave with other items. As well as not knocking up the grain there but at home. Making the cultures there might be risky for you. Later I'll ask you on how you like to divide your cultures and how far the dision into cells you damage without killing them?
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Leviticus969



Registered: 07/01/12
Posts: 939
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c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
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Size has as much to do with moisture content and care than anything else, so it's not a good indicator of what to clone. In addition, with P cubensis, smaller fruits generally have more potency per gram of material than larger fruits.
You clone for the qualities you want in future flushes. Since clusters tend to produce more product in a given area, I prefer to clone a fruit from clusters. Of course there's other qualities that you also select for as well.
If growing a Psilocybe species, clone a fast growing, good pinning, potent fruit from a cluster. Since potency is largely subjective, sample a small part of your crop early, and if you like what you eat, clone a fruit from a large cluster the next morning. RR
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Leviticus969



Registered: 07/01/12
Posts: 939
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Here it is.... Quoted from the legend himself. Check yourself
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The biggest problem with multispore inoculation from syringes is the constant reports of zero potency. It's rare to actually get a total non-fruiting strain, but non-potent strains seem pretty common. A search here for low or no potency will return hundreds of threads on the subject.
When you isolate strains on agar, you grow each one to fruiting, carefully labeling each petri dish after you cut a wedge from it to inoculate grains. The petri dishes then go into the refrigerator for storage until you fruit each strain. When you do and determine the one superstrain that gives awesome yields and great potency, you go back to that dish, save it on a master culture slant, and grow it forever. The odds of getting a superstrain with syringes is very low and might take many years of trying. Then, when you actually get one of those, you realize you just ate them all and have nothing to clone with to keep going. This is why we use agar; you can do in a couple of months what it might take 20 years to find by accident using multispore inoculation. RR
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Leviticus969



Registered: 07/01/12
Posts: 939
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Quote:
RogerRabbit said:
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passenger said: am i cloning just the characteristics of the mushroom or am i cloning the potency as well? thanks.
What makes potency not a characteristic? RR
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:01 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Leviticus969 said: c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
You cannot be this dense. In case you are, it's because potency is not the same as chemicals that can have different effects from each other. Potency is a number. It scales.. linearly. Like fucking numbers god damn you. You can't drink beer until you feel the effects of LSD.
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Srirachi
Mold Hand



Registered: 10/18/05
Posts: 11,411
Loc: Fare Thee Well.
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Reading gives you information, doing gives you knowledge. Sort of like the difference between reading the phone book and Walden.
RR probably also has some quotes out there that give what you posted context. There are no "super-potent cubes" but there are people fucked up enough that they can't even achieve normal potency. You don't clone weak shrooms is all RR says here. Cloning doesn't result in more potency over time; it leads to reduced potency.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Saying that psilocin psilocybin and the few other indoles that may cause psychoactive effects can be bad or good in some mushrooms and not others. Well it's by far the most fucking retarded thing ive seen posted here this month.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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doesnt even read the rules well enough to know he could get banned for triple posting, much less the basics of cultivation. just googles "site:shroomery.org potency genetic" , nevermind the people trying to explain the relationship between those two terms 
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Leviticus969 said: c10 again I ask you.... If set and setting are the important factors in determining a trip than why do people clone for potency.
you are still completely missing the point. yes, we clone for potency, among many other things. that is because there can be HUGE fluctuation in the levels of alkaloids between strains of cubensis.
genetics is only one part though, that only determines POTENTIAL. just like an NBA player's kid might have the potential to be great at basketball, in the right conditions he could also suck, and never reach that potential. A strain could be particularly potent (prone to high levels of alkaloids), but all that means is it has the potential to be potent, not that it will be potent. you are ignoring every single other part of the equation, and you dont even understand the genetics part
dosage of alkaloids certainly affects the intensity of the trip, and some fruits will have more alkaloids than others. but whether it is a "good trip" or "bad trip" is totally subjective, just like "good cheese" or "bad cheese"
i guarantee you, even with the mushrooms you think ensure a "good trip", if i were to chain you in a closet and abuse you every way i could think of for 3-4 months, then feed you some of those "good trip shrooms" and you would be miserable and terrified, the definition of bad set and setting, the definition of a bad trip
set and setting determines whether you will have a good or bad trip, nothing else. genetics AND environment/conditions determine the amount of alkaloids present, which is a broad indicator of perceived "potency"
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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So take his advice and isolate or clone for your consistent potency and good trip vibe mushrooms. Quit dicking around with cakes and ms if your taste is so refined.
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Leviticus969



Registered: 07/01/12
Posts: 939
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wow how about instead of asking me to provide proof, how about you provide proof and I can take a shit on it. It works both ways. You think my info is shit and I think yours is too. It's a win win situation.
if everyone were to jump off a bridge would you also follow? = sounds a lot like you guys.
The point I was trying to make is that there are A SHIT TON of zero magic, bunk shroom trip reports all over the psychedelic forum and youtube videos. This is probably cuz dumbasses grow shit and sell it to unaware people. Are you guys guilty of this or what? Is this why most of yall so stubborn to any different research and ideas? pssh must be. It's no wonder yall blame shit on set and setting when your shrooms are just bunk.
Edited by Leviticus969 (10/22/16 10:32 PM)
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 12:01 PM)
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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A bunk shroom isn't necessarily the growers fault. The potency of a shroom is like... Alcohol content. I guess cause I have to explain it to someone like you. A bunk shroom is like a can of beer. To achieve the results of let's say, half a fifth of tequila, you need to drink like 15 cans of beer. You will still get just as drunk though technically, because you had to consume the same amount of ethyl alcohol, but you just had to take a lot more to get there. But you will still be just as drunk.
And then have you ever had that 1 tequila drunk where you cried and or pissed yourself and or vommed and had a terrible night? Does that mean it was a bad batch of tequila?
 You just binged it irresponsibly and took it the wrong way, had a fight with your girlfriend, just had a shit night. But your friend who drank the same tequila was just fine.
-------------------- AMU Q&A
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
Leviticus969 said: wow how about instead of asking me to provide proof, how about you provide proof and I can take a shit on it. It works both ways. You think my info is shit and I think yours is too. It's a win win situation.
if everyone were to jump off a bridge would you also follow? = sounds a lot like you guys.
The point I was trying to make is that there are A SHIT TON of zero magic, bunk shroom trip reports all over the psychedelic forum and youtube videos. This is probably cuz dumbasses grow shit and sell it to unaware people. Are you guys guilty of this or what? Is this why most of yall so stubborn to any different research and ideas? pssh must be. It's no wonder yall blame shit on set and setting when your shrooms are just bunk.
Nobody here is a dealer and we are all well aware of how to avoid bunk fruits. If you would shut your hole and listen you could do it to. Them YouTube morons fail cause they do the same shit you are doing. I have grown bunk fruits. As soon as a culture came out bunk it hit the trash. When proven good it went in a slant. Then grow the good culture going forward. Grow like a noob get noob results. As long as your trying to grow "good trip shrooms" or "dealer grade" based on variety or looks, the possibility of fail will be strong.
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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this thread is hilarious yeah, me and pasty and inoc grow shitty shrooms, who are we? lmao
this guy is just an idiot, plain and simple.
as i and many others have explained, a basic grasp of psychology and biology is all it takes to prove that set and setting determines whether a trip will be good or bad. we have repeatedly explained that there is a variation in the levels of potential potency between strains, and the way dosage impacts the experience. most of all, the fact that trips are a subjective experience.
if this guy was anywhere north of retarded he would realize what is wrong with thinking there are "good trip shrooms" and "bad trip shrooms"
like i said before:
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i guarantee you, even with the mushrooms you think ensure a "good trip", if i were to chain you in a closet and abuse you every way i could think of for 3-4 months, then feed you some of those "good trip shrooms" and you would be miserable and terrified, the definition of bad set and setting, the definition of a bad trip
im just glad inoc bothered mentioning this guy's infamous burn pile, this shit is the funniest/dumbest thread ive seen in a while, even beats the guy with the shed and handgun the other day lol
we love diverse thought, debate, intelligent discussion, and real experimentation here, check out my lifecycle questions thread if you want to see some of the stuff we think about. this is certainly not a homogeneous group of opinions in general, we have different opinions on everything from grain soaking, spawning at fruit, getting isolates, and a thousand other things. but it seems like we can all agree on what an idiot this guy is. if he was going connect the dots he would have at least quit talking shit by now
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Leviticus969



Registered: 07/01/12
Posts: 939
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lol i'm here all day. You guys are ass backwards as fuck. And yes, I am calling you out on your stupid shit. You guys grow bunk shit deal with it LOLOLOLOL
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SloppyJoseph said: A bunk shroom isn't necessarily the growers fault. The potency of a shroom is like... Alcohol content. I guess cause I have to explain it to someone like you. A bunk shroom is like a can of beer. To achieve the results of let's say, half a fifth of tequila, you need to drink like 15 cans of beer. You will still get just as drunk though technically, because you had to consume the same amount of ethyl alcohol, but you just had to take a lot more to get there. But you will still be just as drunk.
And then have you ever had that 1 tequila drunk where you cried and or pissed yourself and or vommed and had a terrible night? Does that mean it was a bad batch of tequila?
 You just binged it irresponsibly and took it the wrong way, had a fight with your girlfriend, just had a shit night. But your friend who drank the same tequila was just fine.
Ok so tequila is on the same level as cubes now? Bruh you're dumb as fuck. Where's your scientific evidence bruh? IS heroin on the same level as cubes also? durrrrrrrrrrhhhhh
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Nobody here is a dealer and we are all well aware of how to avoid bunk fruits. If you would shut your hole and listen you could do it to. Them YouTube morons fail cause they do the same shit you are doing. I have grown bunk fruits. As soon as a culture came out bunk it hit the trash. When proven good it went in a slant. Then grow the good culture going forward. Grow like a noob get noob results. As long as your trying to grow "good trip shrooms" or "dealer grade" based on variety or looks, the possibility of fail will be strong.
I don't think no one here is aware of how to avoid bunk fruits let alone be able to tell what bunk is. This whole thread proves it. And yeah, i'm sure all yall grow tubs upon tubs for personal use. Who the fuck do you guys think ya tryna fool.
It's better talking to a brick wall than talking to stubborn narrowminded idiots. Mush cult has become such a joke since RR and RK left. How pathetic.
No wonder why people leave this hobby after a grow or 2. Dealing with you hateful misinformed fucktards on here is sad. Just sad.
Edited by Leviticus969 (10/23/16 01:16 AM)
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c10h12n2o
serial dilutor



Registered: 01/21/15
Posts: 3,200
Loc: the abyss
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yeah, me, pasty, and inoc grow the bunkest shrooms around, we are trying to deal with it
check out some of my bunk ass shrooms from this month:
 
thats 1 flush from a 66qt tub, 3498 grams from 1 flush from 1 tub. 1 fruit weighed 295 grams. and the fuckin guy who is still doing cakes is gonna tell me whats bunk
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  C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide "Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing." "Convictions are more dangerous enemies of truth than lies" ― Friedrich Nietzsche
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Leviticus969



Registered: 07/01/12
Posts: 939
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Those are nasty ass looking shrooms and i can tell they suck like shit just from looking at it. Look at all those nasty as holes in em. It's deformed like your brain. You act like I've never grown tubs or grown huge fruits just cuz I dont post all my grows. Wow you are desperate. Also, fyi mr smarty pants, it doesn't fucking matter what substrate you grow shrooms on. lmao but you believe so since that's what everybody else believes. If you want me to find the rr quote on that i'll be glad to.
And what's up with that dick slapping pic? Are you a raging homo or what? That's disgusting. Wow there are such degenerates on here.
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