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InvisibleInocuole
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Re: Lab access [Re: Pastywhyte]
    #23760624 - 10/22/16 11:11 AM (7 years, 10 months ago)

Oh SHIT is this bad trip cube bonfire guy??  :rofl:  The dude who could stand to learn the most about genetics, basically?


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Coir-ca-cola
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Invisibleamidogen
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Re: Lab access [Re: Inocuole]
    #23760630 - 10/22/16 11:13 AM (7 years, 10 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (06/21/18 11:30 AM)

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InvisibleInocuole
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Re: Lab access [Re: amidogen] * 3
    #23760652 - 10/22/16 11:20 AM (7 years, 10 months ago)

Well this guy is 6Silent9Knight6 and that's why he's being shitty to me, because we've had run ins before.  Hadn't noticed til now. :shrug: Basically he believed that some mushrooms gave good trips and some gave bad trips, and he thought he could tell by looking at them, so he would allegedly burn piles of mushrooms that would have given bad trips.  Naturally many laughs were had.
https://www.shroomery.org/forums/showflat.php/Number/21897057#21897057

He's a charmer as you can see:

https://www.shroomery.org/forums/showflat.php/Number/21975841#21975841


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Invisibledankington
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Re: Lab access [Re: Inocuole]
    #23760659 - 10/22/16 11:24 AM (7 years, 10 months ago)

:loldongs:

I only knew because his title is still 'SPUN' :lol:

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InvisiblebodhisattaMDiscordReddit
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Re: Lab access [Re: dankington]
    #23760681 - 10/22/16 11:33 AM (7 years, 10 months ago)

Good catch.

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Invisibleirequirechocolate
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Re: Lab access [Re: bodhisatta]
    #23760734 - 10/22/16 11:53 AM (7 years, 10 months ago)

Thanks for all the info, guys. I think the reiteration of "agar agar agar" was good, if anything because it took me to reading a ton of stuff by SpitBallJedi and really has my gears turning, which 'feels good man.' I was actually dreaming about monotubs last night, no joke.

I hope no one on here thought I was carrying any animosity around, but if so, saaawryyy. Also, I aint trying to be like "I'm in science so I'm smarter." Not by a long stretch. Just introducing myself and setting up a background for the most part.

Aside from that, I'll be careful. Thanks for the concern, the accumulated knowledge, and the entertainment. Cheers.

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Invisiblec10h12n2o
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Re: Lab access [Re: Inocuole]
    #23761024 - 10/22/16 01:49 PM (7 years, 10 months ago)

Quote:

Inocuole said:
Quote:

amidogen said:

B) the only thing your lab really gives on the cult side is a good place to knock up agar plates or grain jars with your clean agar wedges...but you could do it at home for much less risk.



Gonna have to disagree.  A good place to inoculate agar and grain is a place where you won't have to move it afterwards.  Imagine having to drive your inoculated media back and forth from home and the lab.  Insanely risky, in more ways than one.

OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about.  Inevitably, it works out less than well for most.  No matter what background you have, even if you've grown other mushrooms, to understand what we're doing here you have to forget everything you know, even if you had to pay money to know it.  Yes that sucks when free information threatens to invalidate something you invested in, but large parts of life are just a joke in that way, unfortunately.

So to actually answer your question, I think the DNA sequencing and confirmation of certain features of isolates and clones, etc, like mentioned, would be a big help, but not if it means you're going to end up on the news.  We're more interested in just having members who can grow mushrooms well and teach others the way than people who are willing to risk their career, their freedom, and our obscurity.  I'm sure if you absorb the info here and then draw the connections from that to what you already know, after you've had some time to build up the fomer, at that time you'll be able to make some truly valuable contributions here.

I genuinely hope you make it that far.  People like me can be offputting but as soon as you've been around the block for a bit you'll understand exactly where I'm coming from, and may even re-evaluate your take on my approach.  I can tell you now I don't pay anyone off to respect any of my opinions.  :shrug:




lol holy shit inoc, have you been writing campaign speeches or something? very succinct, polite, and comprehensive reply, extremely well put, you sound like a lawyer or something haha

even in the depths of my ignorance, a post like that would be enough to make me take a long hard look at my assumptions. nice job buddy, and spot-fucking-on
:highfive1:

though i am only saying this becaus inoc pays me  :money:


LMFAO here is a quote from his thread:

Quote:

Leviticus969 said:
Quote:

lol... To answer your question... I burn all my grows in the fire pit in my back yard. I would not ever sell or give away bad shrooms with disgusting trips. I hate taking those trips myself and I would not want others to have those same trips. Then you get people on youtube posting their bad trips and disgracing the mushroom name

But yeah... I know for sure bad trips don't come from my head, set or setting. It's genetics.

If you look at the pictures of the potent strain i posted above... flush after flush after flush the potency was bomb as fuck and IDENTICAL. No matter where i ate them or how i was feeling. It was really out of this world magic.









he knows FOR SURE that set and setting have nothing to do with the way his brain interprets those weird shroomy neurotransmitter-like alkaloids: its definitely the genetics of the organism that produced them which modulates his experience. 

LOL, such confidence. this is why the USA scores in the bottom of the 25 most developed nations for science, math, and language skills, but we score #1 on the question "how well do you think you did on the test?" :rofl:  funny but sad


--------------------

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"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Offlinetump
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Re: Lab access [Re: c10h12n2o] * 1
    #23761058 - 10/22/16 02:04 PM (7 years, 10 months ago)

Idk that setting is most important for tripping. But i can tell a bad trip shrooms are too by looking at them if they aren't stored proper , black molded growing them always a bad time. Other then that i think its impossible to know.

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InvisibleJosex
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Re: Lab access [Re: tump] * 2
    #23761148 - 10/22/16 02:39 PM (7 years, 10 months ago)

Quote:

tump said:
Idk that setting is most important for tripping. But i can tell a bad trip shrooms are too by looking at them if they aren't stored proper , black molded growing them always a bad time. Other then that i think its impossible to know.



:knowwhatnevermind:
Another invaluable insight brought to you by tump...

Seriously, how in the name of fuck do you get black mold on your stored shrooms? Better yet, how come you have the balls to eat something in that state?
And you say bad trips? It's strange you don't end up having a trip to the emergency room one of these days.

How do you dry your mushrooms?

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InvisibleLeviticus969
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Re: Lab access [Re: c10h12n2o]
    #23761201 - 10/22/16 03:01 PM (7 years, 10 months ago)

Quote:

he knows FOR SURE that set and setting have nothing to do with the way his brain interprets those weird shroomy neurotransmitter-like alkaloids: its definitely the genetics of the organism that produced them which modulates his experience.

LOL, such confidence. this is why the USA scores in the bottom of the 25 most developed nations for science, math, and language skills, but we score #1 on the question "how well do you think you did on the test?" :rofl:  funny but sad




If shroom trips are based off of set and setting than we wouldn't be cloning for potency.  :facepalm:

This is the equivalent to saying all weed strains are the same. They're not.... Some weed is literally bunk and some get you high as fuck.

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Invisibleamidogen
see you on the other side

Registered: 05/07/16
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Re: Lab access [Re: Leviticus969]
    #23761296 - 10/22/16 03:40 PM (7 years, 10 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.

Edited by amidogen (04/19/18 12:01 PM)

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OfflineAlCapone2k
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Re: Lab access [Re: amidogen]
    #23761402 - 10/22/16 04:35 PM (7 years, 10 months ago)

Common guys, all of you are respected members here with a lot of knowledge and it seems there was some misunderstanding, no reason to let the thread crash.

The intention of the OP was very noble, since he just wanted to "help" the community with information he thought he can gain because of his equipment. What we found out is, that his equipment is not really necessary for cubensis growth. Nonetheless it was well-intentioned.

Maybe there is some question he could answer with his knowledge, or maybe another one will answer it whith his knowledge. As long as the question is answered, everyone is happy I think :smile:

For example I'd like to hear his or one of the other guys opinion on the inoculation of agar plates. Better to put the loop or swab in the middle of the agar plate (growth from middle to the edges) or to get rid of bacteria better to drag it on with the known Z-form?

I tested both and as expected: The Z-form show mycelium everywhere, I think it will be quite hard to isolate from that plate. The middle-inoculation show a great mycelium growth from the middle heading towards the edges, isolation will be easier, and also no contamns til now (4 days sine inoc).


--------------------
Since I am from Germany, my english is not perfect but I try my best :smile:




My small Agar to Oyster Open Air Grow Trek

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InvisibleLeviticus969
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Re: Lab access [Re: amidogen]
    #23761408 - 10/22/16 04:38 PM (7 years, 10 months ago)

Quote:

amidogen said:
Ok. So following your reasoning it's all about the alkaloid content. You've got three possibilities: psilocybin, psilocin, baeocystin. My questions for you are:

A) what ratio of alkaloids do you think automatically gives you a good trip? Like if I eat shrooms with this alkaloid content and then go watch House on Haunted Hill with a bunch of cops, I'm still gonna be seeing rainbows and be in a blissful state of mind?

B) what the fuck sort of objective evidence do you have for this?

C) how exactly do you quantitatively (or even qualitatively) looking at some mushrooms you've grown and decide by eye the alkaloid content and then determine which you should burn?

:popcorn:





A) Ratio's for the alkaloids for a good trip? Personally, i say fuck it to the ratios and scientific terms for potency. I've never been one to take the institutions status quo too seriously as they are prone to mistakes and unless I'm able to test it out for myself to see it's validity. Psilocybin, psilocin, baeocystin are terms used to gauge potency but here on the erowid site it says itself:

Quote:

Please note that these do not represent average values for widely sampled populations. Just because a mushroom species has some samples that show high or low concentrations does not mean that all mushrooms of that type have levels matching those in this chart.






You can eat a cube off a grow and it'll make you want to commit suicide and you can eat a different cube grow and feel like heaven on earth. How does one measure the Psilocybin, psilocin, baeocystin contents? I don't have lab gear so I don't know. The only way to test it is to eat it (and in my case "sometimes" looks can help since I've grown/eaten a shit ton of cubes and have been able to distinguish somewhat by appearance)

B) Where is my objective proof? Myself. And you're probably going: Well who the hell is this guy?? Well, considering this mycology field is an illegal activity for most places in the world where can you really get solid information other than random growers on the shroomery site... Like I said, nothings set in stone and there's still a LOT more to be known about the mushrooms. But here are a few quotes from RogerRabbit, a well renowned and respectable member of the community even though he's barely here anymore.

Quote:

Good luck.  Science is never settled by popularity contests.

Potency is genetic, and that means that whatever genetics combine into the final mycelial network will determine the potency, provided you provide a medium on which it can grow and be healthy.  After many years of growing on hundreds of different substrates, I can say from personal experience that there's not a bit of difference in potency when growing isolated strains on various substrates.

If one so-called 'strain' was always more potent, and one substrate was always producing fruits with more potency, don't you think we'd only be using those?
RR




Quote:

Cubes are cubes.  With multispore inoculation it's a crap shoot what you're going to get, just like with children.  Saying that PE is always more potent is like saying black people are always better basketball players or that white people are always better golfers.  Genetics fall where they might.  Every family with ten kids has a genius, a retard, a good kid, a hoodlum, and the rest fall in the middle.  It's the same way with 'strains'.  Pick one and grow it.  No cube is always more potent than any other.
RR




And his information is not all correct also as I've had conflicting observations. But for the most part that's what the community is about... Chiming in our experiences and OPINIONS to come to a conclusion.


C) Quantatively/qualitively how do I determine which shrooms are bunk and which are bomb based off of looks?

Well that takes a shit ton of years of growing many different strains. But in my personal opinion, if you want something beautiful, rare, out of the ordinary magic kinda shit than first you'll have to "look" for what stands out before eating.

Here I'll throw in some pictures and let you decide which ones might be the potent boomers ( i just ripped this off of my potency thread so no cheating and if you already saw it than well....)

4 of these pictures are what I consider the mews of pokemon



So yeah to briefly sum it up looks definitely do help but the final most important step is to eat it. And quite frankly, I hate eating shrooms that make me want to commit suicide and believe me I've eaten quite a lot and endured a lot of those negative evil trips. It's a waste of my my life.

A truly euphoric magical strain would have you wanting to shroom out at least once a week. Not once every year.

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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: Leviticus969]
    #23761610 - 10/22/16 05:31 PM (7 years, 10 months ago)

:lolwut:

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InvisibleLeviticus969
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Re: Lab access [Re: Pastywhyte]
    #23761616 - 10/22/16 05:33 PM (7 years, 10 months ago)

:tongue::peace::heart:

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Offlinetump
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Re: Lab access [Re: Leviticus969]
    #23761865 - 10/22/16 06:47 PM (7 years, 10 months ago)

Picture 1,4,5,7 all have high celler mass which means they have higher psilocybin. Do i win? I like pokemon

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InvisibleInocuole
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Re: Lab access [Re: tump]
    #23761957 - 10/22/16 07:16 PM (7 years, 10 months ago)

Shitposts... shitposts everywhere.  Sorry OP.  :shrug:


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Invisibleirequirechocolate
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Re: Lab access [Re: Inocuole]
    #23762035 - 10/22/16 07:41 PM (7 years, 10 months ago)

Quote:

Inocuole said:
Shitposts... shitposts everywhere.  Sorry OP.  :shrug:




No worries. I might turn notifications off for a while though, hah

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Offlinetump
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Re: Lab access [Re: irequirechocolate]
    #23762087 - 10/22/16 08:01 PM (7 years, 10 months ago)

I mean to ask op what project is your lab working on? Also what are your spects on the auto clave you have?

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Invisibleirequirechocolate
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Re: Lab access [Re: tump]
    #23762105 - 10/22/16 08:08 PM (7 years, 10 months ago)

Quote:

tump said:
I mean to ask op what project is your lab working on? Also what are your spects on the auto clave you have?




I'd rather not start calling more attention to my workplace by mentioning the specifics.
The autoclaves are very versatile as to what their setting can be - times/temp/psi/etc. TBH I can't remember what I had it at exactly, but I used a default liquid cycle setting, which is one that prevents sterilized liquids from boiling. I figured I didn't want to mess w/ the moisture within the substrate by over cooking it. The whole process takes ~90min, and then I let the jars cool for 5hrs to prevent scorching the spores to hell during inoculation.

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