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Invisibleirequirechocolate
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Registered: 10/15/16
Posts: 132
Re: Lab access [Re: SloppyJoseph]
    #23759661 - 10/21/16 11:58 PM (7 years, 3 months ago)

Quote:

SloppyJoseph said:
Malted barley would probably get too starchy to be useful for this. Nutritional content doesn't really matter, actually the richer it is the slower the mycelium will colonize.




I see. I don't want to get too ahead of myself, but if I were to try using the spent stuff anyhow, maybe mixed w/ a less starchy grain, what in particular would you recommend reading up on? Some of you guys seem to have some of the better posts bookmarked and ready to share.

And btw thanks for the conversation. This is productive. As I try these things out I'll be sure to share results.


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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: irequirechocolate]
    #23759676 - 10/22/16 12:15 AM (7 years, 3 months ago)

Spent grain has been grown on but yield suffers greatly with cubes, unless of course you have a killer culture. Try it with oysters.


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Invisiblec10h12n2o
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Re: Lab access [Re: Pastywhyte]
    #23759853 - 10/22/16 02:22 AM (7 years, 3 months ago)

a couple of things:

#1 dial back the animosity a little bit, or you will never learn anything. dont look for things to get upset about, try to understand what people are saying. you need to unlearn a LOT of incorrect assumptions, which is where we all start. the sooner you realize what the community has to offer and show a little humility/respect (especially when people are answering your questions :rolleyes:) the sooner you will be up to speed

#2 you need to be careful. whoever is paying for that lab is smart enough to know what you are doing, and it is a bad idea to build a tower of assumptions about what you think people know and care about. you sound like you are itching to get fired/arrested. please be careful, for your sake, and for the sake of our hobby

#3 most of the equipment you mention is not needed for these types of projects. things like a fume hood, sharps disposal, reagent suppliers, etc are completely unrelated to this kind of mycology. any experienced cultivator will have better stuff at their house (a flow hood/SAB, agar making supplies -- dirt cheap btw, people are so funny complaining about the cost of agar, scalpel blades and handles, pressure cooker, parafilm, culture tubes, etc). most of us in Mush-Cult have home labs much better equipped for and suited to magic mushrooms than the lab you are describing

#4 if you want to see some questions that would be awesome for a mycologist, or a geneticist who studies fungi, this is a thread i made on cubensis lifecycle questions. this is the type of stuff that experienced growers wonder about; what you are talking about is just misguided, and any amateur grower could explain why.

#5 i think you will need to learn a lot more of the basics before you will have a good idea of what constitutes a question that is worth bothering a professional with

#6 if you work in a DNA lab, it would be awesome to work out a process for distinguishing strains from one another genetically, mapping where the genetic differences are, or even just being able to determine if samples are genetically identical (ie, if something is REALLY a monoculture). there has been very little published work done on cubensis DNA. but again, you are just going to confuse yourself and anyone listening to you by trying to talk about this stuff before you figure out the basics

warm regards


--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche


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OfflineMushierage
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Registered: 06/29/16
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Re: Lab access [Re: Leviticus969]
    #23759863 - 10/22/16 02:32 AM (7 years, 3 months ago)

Quote:

Leviticus969 said:
Whatever man no one needs that condescending know it all attitude around here get a life. :lol:





Um.. I thought Inocuole gave you a pretty succinct and level headed answer.  And the fact is, it isn't outdated information or incorrect either.  He's right.

I think you're the one with an attitude.  He didn't come in with an attitude but you sure responded with one.



--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Offlinetump
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Registered: 03/17/16
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Re: Lab access [Re: Mushierage]
    #23760008 - 10/22/16 05:09 AM (7 years, 3 months ago)

I have nothing new to add just want to be part of this thread. Congrats on your lab work. Everyone in here as attitude issues buts its ok. But lab workers are very judgemental because there pure agar cost 74 times what the food grade stuff does. Or reading a cultures gene scene while running a electrical current perfectly is the goal in a lab it isn't for normal people. The normal good people of the site are more caring about yeild there nothing with that. So just chill


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Invisibleblindingleaf
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Re: Lab access [Re: tump]
    #23760027 - 10/22/16 05:30 AM (7 years, 3 months ago)

I never tried cubes on spent grain just edibles (and not even a lot, bran easier).  Bw86 did a cool grow with them.  It was sterile bags tho.

Need to use them ASAP and really watch moisture content of ur final mix cause those things are sticky icky

Don't talk down to the regulars, they help way more than you know.  Not every teacher is the same...on here, in school, at jobs, etc. So man up and try to see the big picture of what this forum is about


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinemushroomnoob1981
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Registered: 06/15/15
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Re: Lab access [Re: blindingleaf]
    #23760050 - 10/22/16 05:49 AM (7 years, 3 months ago)

Is anyone in your lab doing mammalian cell culture?  If so,  they'll hate you if you bring anything fungus related in.


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InvisibleInocuole
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Re: Lab access [Re: tump] * 1
    #23760156 - 10/22/16 07:17 AM (7 years, 3 months ago)

Quote:

tump said:
Everyone in here as attitude issues buts its ok.




Some of us even claim to have government grants to grow cubes..


--------------------
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Coir-ca-cola
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InvisibleSrirachi
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Re: Lab access [Re: Leviticus969]
    #23760280 - 10/22/16 08:44 AM (7 years, 3 months ago)

Quote:

Leviticus969 said:
I don't have to be a geneticist to know this?





No, you don't. Ask Alan Rockefeller in the hunting forum if you doubt this; he's a geneticist and will tell you that you don't need him.

Quote:

Leviticus969 said:Did I ask you?





You asked the shroomery, a subset of which is Inocule. So yes, yes you did ask him.


Quote:

Leviticus969 said:Mr Know it all? Have you studied mushrooms all your life? Who are you?






1.) Mr. Knows more than you will do for now.
2.) It is only necessary to study mushrooms for a small part of your life to learn to answer YOUR questions.
3.) He's this guy on the Shroomery who knows more about mushrooms than you.


Quote:

Leviticus969 said:I'm not looking for the cliche status quo responses on here given by random growers that think they know everything when they just recycle outdated information on the shroomery so thanks and have a good day.




For this reason, I think I'll put you on ignore. I seldom put people on ignore. I mean, real assholes like people I don't think enough of to remember the names of right now aren't even on my list. But I'm going to skip the benefit of the doubt and assign you to that category of people who walk into a 20-year-old discussion and think that, with no knowledge of your own, you can just yell "Okay people LISTEN UP, now how the fuck do I do this?!?!" and assemble a roadmap to "super potent cubes" that no experienced cultivator before you has managed to glean from all their studies of this site and reality.

IOW, accept the help you asked for with a modicum of humility, or fuck off.

Best,
Some random guy who regurgitates info from others


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Invisibleblindingleaf
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Re: Lab access [Re: Srirachi] * 2
    #23760312 - 10/22/16 09:08 AM (7 years, 3 months ago)

I can Only really get on here in the AM and u can tell when inoc isn't on by the amount of vague, foolish, and wrong answers in threads that aren't checked :respect:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleInocuole
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Re: Lab access [Re: blindingleaf]
    #23760326 - 10/22/16 09:15 AM (7 years, 3 months ago)

Sorry man, definitely been slackin, other shit picked up a bit.  My posts per day graph is gonna take a hit for sure.  It's been getting weird around here though, it's like even relatively normal people are catching the crazies and saying/doing dumb shit.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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Invisibleamidogen
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Registered: 05/07/16
Posts: 1,782
Re: Lab access [Re: Srirachi] * 1
    #23760327 - 10/22/16 09:16 AM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:00 PM)


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InvisibleInocuole
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Re: Lab access [Re: amidogen] * 1
    #23760347 - 10/22/16 09:28 AM (7 years, 3 months ago)

Quote:

amidogen said:

B) the only thing your lab really gives on the cult side is a good place to knock up agar plates or grain jars with your clean agar wedges...but you could do it at home for much less risk.



Gonna have to disagree.  A good place to inoculate agar and grain is a place where you won't have to move it afterwards.  Imagine having to drive your inoculated media back and forth from home and the lab.  Insanely risky, in more ways than one.

OP I'm not trying to give you a hard time in particular but you have to understand we get people from all kinds of biology and engineering backgrounds who come in here and they really feel like they can bring something to the community by applying what they know to a thing they know nothing about.  Inevitably, it works out less than well for most.  No matter what background you have, even if you've grown other mushrooms, to understand what we're doing here you have to forget everything you know, even if you had to pay money to know it.  Yes that sucks when free information threatens to invalidate something you invested in, but large parts of life are just a joke in that way, unfortunately.

So to actually answer your question, I think the DNA sequencing and confirmation of certain features of isolates and clones, etc, like mentioned, would be a big help, but not if it means you're going to end up on the news.  We're more interested in just having members who can grow mushrooms well and teach others the way than people who are willing to risk their career, their freedom, and our obscurity.  I'm sure if you absorb the info here and then draw the connections from that to what you already know, after you've had some time to build up the fomer, at that time you'll be able to make some truly valuable contributions here.

I genuinely hope you make it that far.  People like me can be offputting but as soon as you've been around the block for a bit you'll understand exactly where I'm coming from, and may even re-evaluate your take on my approach.  I can tell you now I don't pay anyone off to respect any of my opinions.  :shrug:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
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InvisibleLeviticus969
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Re: Lab access [Re: Srirachi]
    #23760432 - 10/22/16 10:03 AM (7 years, 3 months ago)

lol you guys can bitch about it all you want but at the end of the day it's like... just your opinion dude.  :facepalm:

Quote:

Don't talk down to the regulars, they help way more than you know.  Not every teacher is the same...on here, in school, at jobs, etc. So man up and try to see the big picture of what this forum is about




Just because I don't post on here regularly doesn't mean i'm some stupid newb that needs to be talked to in a condescending manner. Get over yourselves. And I certainly don't need a bunch of people with their "opinions" telling me I know nothing just because they don't even know me. That's pretty ignorant and arrogant. what a buncha chumps

I asked the OP, who happened to have a shit ton of genetic information piled up, a simple question for shits and giggles. It wasn't like it's some mad scientist golden information no one could ever find with the search engine.


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OfflinePortabellaFella 1
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Re: Lab access [Re: Leviticus969] * 1
    #23760449 - 10/22/16 10:06 AM (7 years, 3 months ago)

You should have used the search engine then. I know, I know fuck me


--------------------
I would like to acquire anything I don’t have


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Lab access [Re: PortabellaFella 1]
    #23760471 - 10/22/16 10:19 AM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:00 PM)


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InvisibleLeviticus969
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Re: Lab access [Re: amidogen]
    #23760484 - 10/22/16 10:25 AM (7 years, 3 months ago)

That's not the point. The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas. Nothing is ever set in stone and things can always be improved upon.

I asked the question for 2 reasons.... to first let the OP know about 1 of the things he can easily test for himself since he has access to all that lab gear. and 2, to let me, or anyone else on here, know the results of his experiments so that the whole community "might" learn something new.


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: Lab access [Re: Leviticus969] * 1
    #23760500 - 10/22/16 10:31 AM (7 years, 3 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 12:00 PM)


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InvisibleInocuole
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Re: Lab access [Re: Leviticus969]
    #23760504 - 10/22/16 10:32 AM (7 years, 3 months ago)

Quote:

Leviticus969 said:
The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas.





This is pretty ironic considering we're talking about readily accepting wrong information while simultaneously denying correct information.  Great! The system works!  If you understood what appeal to authority meant you'd understand the logical fallacy in the very first interaction.  Things just went downhill from there due to what is undoubtedly the result of a defensive and fragile ego.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisiblePastywhyteMDiscord
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Re: Lab access [Re: Leviticus969]
    #23760613 - 10/22/16 11:06 AM (7 years, 3 months ago)

Quote:

Leviticus969 said:
That's not the point. The point is to be readily acceptable of any information from anybody, expert or newb in order to either confirm or test new ideas. Nothing is ever set in stone and things can always be improved upon.

I asked the question for 2 reasons.... to first let the OP know about 1 of the things he can easily test for himself since he has access to all that lab gear. and 2, to let me, or anyone else on here, know the results of his experiments so that the whole community "might" learn something new.




So it has nothing to do with your mission to avoid growing "bad trip" mushrooms? Wasn't that always your bag? To find some variety that always grew "good trip" cubes instead of the "bad trip" ones.


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