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Offlinegekkogecko
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Liquid Culture from Master Culture Slant
    #23757686 - 10/21/16 11:05 AM (7 years, 4 months ago)

Howdy

I'm making my own liquid culture for the first time. I have several slants in my fridge. I was wondering anyone has just taken a piece of myc out of a MCS and put it in the LC jar.  I know there's one technique of putting water on a colonized agar dish, swirling it around, sucking up the slurry into a sterile syringe, and plunging into the LC. This seems like it would be fastest, But I'm a week away from having a colonized dish. I would like to skip a step and just go straight from the slant. Is there any reason why this wouldn't work out?

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Offlinetump
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Re: Liquid Culture from Master Culture Slant [Re: gekkogecko]
    #23757689 - 10/21/16 11:07 AM (7 years, 4 months ago)

If you cut it clean and fast i dont see why not

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Re: Liquid Culture from Master Culture Slant [Re: tump]
    #23757730 - 10/21/16 11:21 AM (7 years, 4 months ago)

I prefer to plate first for two reasons. One is that if the slant was compromised and is contamed you could at least see it before going to LC. The second is that after long storage the culture often benefits from a few transfers and media rotation to bring up the vigor. Going straight to expansion might hurt performance.

Cutting corners will not always result in fail but when it does you will regret it.

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